Gene:
Gene Symbol:
NRIP1
Gene Name:
nuclear receptor interacting protein 1
Original Clone ID:
ccsbBroadEn_07208
Keyword:
PERFECT_MATCH
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00623071
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGACTCATGGAGAAGAGCTTGGCTCTGATGTGCACCAGGATTCTATTGTTTTAACTTAC
CTAGAAGGATTACTAATGCATCAGGCAGCAGGGGGATCAGGTACTGCCGTTGACAAAAAG
TCTGCTGGGCATAATGAAGAGGATCAGAACTTTAACATTTCTGGCAGTGCATTTCCCACC
TGTCAAAGTAATGGTCCAGTTCTCAATACACATACATATCAGGGGTCTGGCATGCTGCAC
CTCAAAAAAGCCAGACTGTTGCAGTCTTCTGAGGACTGGAATGCAGCAAAGCGGAAGAGG
CTGTCTGATTCTATCATGAATTTAAACGTAAAGAAGGAAGCTTTGCTAGCTGGCATGGTT
GACAGTGTGCCTAAAGGCAAACAGGATAGCACATTACTGGCCTCTTTGCTTCAGTCATTC
AGCTCTAGGCTGCAGACTGTTGCTCTGTCACAACAAATCAGGCAGAGCCTCAAGGAGCAA
GGATATGCCCTCAGTCATGATTCTTTAAAAGTGGAGAAGGATTTAAGGTGCTATGGTGTT
GCATCAAGTCACTTAAAAACTTTGTTGAAGAAAAGTAAAGTTAAAGATCAAAAGCCTGAT
ACGAATCTTCCTGATGTGACTAAAAACCTCATCAGAGATAGGTTTGCAGAGTCTCCTCAT
CATGTTGGACAAAGTGGAACAAAGGTCATGAGTGAACCGTTGTCATGTGCTGCAAGATTA
CAGGCTGTTGCAAGCATGGTGGAAAAAAGGGCTAGTCCTGCCACCTCACCTAAACCTAGT
GTTGCTTGTAGCCAGTTAGCATTACTTCTGTCAAGCGAAGCCCATTTGCAGCAGTATTCT
CGAGAACACGCTTTAAAAACGCAAAATGCAAATCAAGCAGCAAGTGAAAGACTTGCTGCT
ATGGCCAGATTGCAAGAAAATGGCCAGAAGGATGTTGGCAGTTACCAGCTCCCAAAAGGA
ATGTCAAGCCATCTTAATGGTCAGGCAAGAACATCATCAAGCAAACTGATGGCTAGCAAA
AGTAGTGCTACAGTGTTTCAAAATCCAATGGGTATCATTCCTTCTTCCCCTAAAAATGCA
GGTTATAAGAACTCACTGGAAAGAAACAATATAAAACAAGCTGCTAACAATAGTTTGCTT
TTACATCTTCTTAAAAGCCAGACTATACCTAAGCCAATGAATGGACACAGTCACAGTGAG
AGAGGAAGCATTTTTGAGGAAAGTAGTACACCTACAACTATTGATGAATATTCAGATAAC
AATCCTAGTTTTACAGATGACAGCAGTGGTGATGAAAGTTCTTATTCCAACTGTGTTCCC
ATAGACTTGTCTTGCAAACACCGAACTGAAAAATCAGAATCTGACCAACCTGTTTCCCTG
GATAACTTCACTCAATCCTTGCTAAACACTTGGGATCCAAAAGTCCCAGATGTAGATATC
AAAGAAGATCAAGATACCTCAAAGAATTCTAAGCTAAACTCACACCAGAAAGTAACACTT
CTTCAATTGCTACTTGGCCATAAGAATGAAGAAAATGTAGAAAAAAACACCAGCCCTCAG
GGAGTACACAATGATGTGAGCAAGTTCAATACACAAAATTATGCAAGGACTTCTGTGATA
GAAAGCCCCAGTACAAATCGGACTACTCCAGTGAGCACTCCACCTTTACTTACATCAAGC
AAAGCAGGGTCTCCCATCAATCTCTCTCAACACTCTCTGGTCATCAAATGGAATTCCCCA
CCATATGTCTGCAGTACTCAGTCTGAAAAGCTAACAAATACTGCATCTAACCACTCAATG
GACCTTACAAAAAGCAAAGACCCACCAGGAGAGAAACCAGCCCAAAATGAAGGTGCACAG
AACTCTGCAACGTTTAGTGCCAGTAAGCTGTTACAAAATTTAGCACAATGTGGAATGCAG
TCATCCATGTCAGTGGAAGAGCAGAGACCCAGCAAACAGCTGTTAACTGGAAACACAGAT
AAACCGATAGGTATGATTGATAGATTAAATAGCCCTTTGCTCTCAAATAAAACAAATGCA
GTTGAAGAAAATAAAGCATTTAGTAGTCAACCAACAGGTCCTGAACCAGGGCTTTCTGGT
TCTGAAATAGAAAATCTGCTTGAAAGACGTACTGTCCTCCAGTTGCTCCTGGGGAACCCC
AACAAAGGGAAGAGTGAAAAAAAAGAGAAAACTCCCTTAAGAGATGAAAGTACTCAGGAA
CACTCAGAGAGAGCTTTAAGTGAACAAATACTGATGGTGAAAATAAAATCTGAGCCTTGT
GATGACTTACAAATTCCTAACACAAATGTGCACTTGAGCCATGATGCTAAGAGTGCCCCA
TTCTTGGGTATGGCTCCTGCTGTGCAGAGAAGCGCACCTGCCTTACCAGTGTCCGAAGAC
TTTAAATCGGAGCCTGTTTCACCTCAGGATTTTTCTTTCTCCAAGAATGGTCTGCTAAGT
CGATTGCTAAGACAAAATCAAGATAGTTACCTGGCAGATGATTCAGACAGGAGTCACAGA
AATAATGAAATGGCACTTCTAGAATCAAAGAATCTTTGCATGGTCCCTAAGAAAAGGAAG
CTTTATACTGAGCCATTAGAAAATCCATTTAAAAAGATGAAAAACAACATTGTTGATGCT
GCAAACAATCACAGTGCCCCAGAAGTACTGTATGGGTCCTTGCTTAACCAGGAAGAGCTG
AAATTTAGCAGAAATGATCTTGAATTTAAATATCCTGCTGGTCATGGCTCAGCCAGCGAA
AGTGAACACAGGAGTTGGGCCAGAGAGAGCAAAAGCTTTAATGTTCTGAAACAGCTGCTT
CTCTCAGAAAACTGTGTGCGAGATTTGTCCCCGCACAGAATTAACTCTGTGGCTGACAGT
AAAAAGAAAGGACACAAAAATAATGTGACCAACAGCAAACCTGAATTTAGCATTTCTTCT
TTAAATGGACTGATGTACAGTTCCACTCAGCCCAGCAGTTGCATGGATAACAGGACATTT
TCATACCCAGGTGTAGTAAAAACTCCTGTGAGTCCTACTTTCCCTGAGCACTTGGGCTGT
GCAGGGTCTAGACCAGAATCTGGGCTTTTGAATGGGTGTTCCATGCCCAGTGAGAAAGGA
CCCATTAAGTGGGTTATCACTGATGCGGAGAAGAATGAGTATGAAAAAGACTCTCCAAGA
TTGACCAAAACCAACCCAATACTATATTACATGCTTCAAAAAGGAGGCAATTCTGTTACC
AGTCGAGAAACACAAGACAAGGACATTTGGAGGGAGGCTTCATCTGCTGAAAGTGTCTCA
CAGGTCACAGCCAAAGAAGAGTTACTTCCTACTGCAGAAACGAAAGCTTCTTTCTTTAAT
TTAAGAAGCCCTTACAATAGCCATATGGGAAATAATGCTTCTCGCCCACACAGCGCAAAT
GGAGAAGTTTATGGACTTCTGGGAAGCGTGCTAACGATAAAGAAAGAATCAGAATAA
Protein sequence predicted based on nucleotide sequence
MTHGEELGSDVHQDSIVLTYLEGLLMHQAAGGSGTAVDKKSAGHNEEDQNFNISGSAFPT CQSNGPVLNTHTYQGSGMLHLKKARLLQSSEDWNAAKRKRLSDSIMNLNVKKEALLAGMV DSVPKGKQDSTLLASLLQSFSSRLQTVALSQQIRQSLKEQGYALSHDSLKVEKDLRCYGV ASSHLKTLLKKSKVKDQKPDTNLPDVTKNLIRDRFAESPHHVGQSGTKVMSEPLSCAARL QAVASMVEKRASPATSPKPSVACSQLALLLSSEAHLQQYSREHALKTQNANQAASERLAA MARLQENGQKDVGSYQLPKGMSSHLNGQARTSSSKLMASKSSATVFQNPMGIIPSSPKNA GYKNSLERNNIKQAANNSLLLHLLKSQTIPKPMNGHSHSERGSIFEESSTPTTIDEYSDN NPSFTDDSSGDESSYSNCVPIDLSCKHRTEKSESDQPVSLDNFTQSLLNTWDPKVPDVDI KEDQDTSKNSKLNSHQKVTLLQLLLGHKNEENVEKNTSPQGVHNDVSKFNTQNYARTSVI ESPSTNRTTPVSTPPLLTSSKAGSPINLSQHSLVIKWNSPPYVCSTQSEKLTNTASNHSM DLTKSKDPPGEKPAQNEGAQNSATFSASKLLQNLAQCGMQSSMSVEEQRPSKQLLTGNTD KPIGMIDRLNSPLLSNKTNAVEENKAFSSQPTGPEPGLSGSEIENLLERRTVLQLLLGNP NKGKSEKKEKTPLRDESTQEHSERALSEQILMVKIKSEPCDDLQIPNTNVHLSHDAKSAP FLGMAPAVQRSAPALPVSEDFKSEPVSPQDFSFSKNGLLSRLLRQNQDSYLADDSDRSHR NNEMALLESKNLCMVPKKRKLYTEPLENPFKKMKNNIVDAANNHSAPEVLYGSLLNQEEL KFSRNDLEFKYPAGHGSASESEHRSWARESKSFNVLKQLLLSENCVRDLSPHRINSVADS KKKGHKNNVTNSKPEFSISSLNGLMYSSTQPSSCMDNRTFSYPGVVKTPVSPTFPEHLGC AGSRPESGLLNGCSMPSEKGPIKWVITDAEKNEYEKDSPRLTKTNPILYYMLQKGGNSVT SRETQDKDIWREASSAESVSQVTAKEELLPTAETKASFFNLRSPYNSHMGNNASRPHSAN GEVYGLLGSVLTIKKESE*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223
Synonyms:
pDONR223, pENTR223-XnCDSYn
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp):
2815
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Mark Vidal
Publications:
PMID:
15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
bacterial origin of replication
2220
2753
gene fragment
pas
5p stem of pBR primose assembly site
63
73
gene fragment
pas prime
3p stem of pBR pas(L)
105
116
recombination site
attL1
attL1 recombination site
570
665
recombination site
attL2
attL2 recombination site
793
696
selectable marker
SpnR
spectinomycin resistance gene
1062
2072
trxn termination sequence
T1-T2
rrnBT1 and T2 terminators
510
224
