Gene:
Gene Symbol:
LTBP2
Gene Name:
latent transforming growth factor beta binding protein 2
Original Clone ID:
ccsbBroadEn_06541
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00623098
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGAGGCCGCGGACCAAAGCCCGCAGCCCGGGGCGCGCCCTGCGGAACCCCTGGAGAGGC
TTCCTGCCGCTCACCCTGGCTCTCTTCGTGGGCGCGGGTCATGCCCAAAGGGACCCCGTA
GGGAGATACGAGCCGGCTGGTGGAGACGCGAATCGACTGCGGCGCCCTGGGGGCAGCTAC
CCGGCAGCGGCTGCAGCCAAGGTGTACAGTCTGTTCCGGGAGCAGGACGCGCCTGTCGCG
GGCTTGCAGCCCGTGGAGCGGGCCCAGCCGGGCTGGGGGAGCCCCAGGAGGCCCACCGAG
GCGGAGGCCAGGAGGCCGTCCCGCGCGCAGCAGTCGCGGCGTGTCCAGCCACCTGCGCAG
ACCCGGAGAAGCACTCCCCTGGGCCAGCAGCAACCAGCACCCCGGACCCGGGCCGCGCCG
GCTCTCCCACGCCTGGGGACCCCACAGCGGTCTGGGGCTGCGCCCCCAACCCCGCCGCGA
GGGCGACTCACGGGGAGGAACGTCTGCGGGGGACAGTGCTGCCCAGGATGGACAACAGCA
AACAGCACCAACCACTGTATCAAACCCGTTTGCGAGCCGCCGTGCCAGAACCGGGGCTCC
TGCAGCCGCCCGCAGCTCTGTGTCTGCCGCTCTGGTTTCCGTGGAGCCCGCTGCGAGGAG
GTCATTCCCGATGAGGAATTTGACCCCCAGAACTCCAGGCTGGCACCTCGACGCTGGGCC
GAGCGTTCACCCAACCTGCGCAGGAGCAGTGCGGCTGGAGAGGGCACCTTGGCCAGAGCA
CAGCCGCCAGCACCACAGTCGCCGCCCGCACCACAGTCGCCACCAGCTGGGACCCTGAGT
GGCCTCAGCCAGACCCACCCTTCCCAGCAGCACGTGGGGTTGTCCCGCACTGTCCGACTT
CACCCGACTGCCACGGCCAGTAGCCAGCTCTCTTCCAACGCCCTGCCCCCGGGACCAGGC
CTTGAGCAGAGAGATGGCACCCAACAGGCGGTACCTCTGGAGCACCCCTCATCCCCCTGG
GGGCTGAACCTCACGGAGAAAATCAAGAAGATCAAGATCGTCTTCACTCCCACCATCTGC
AAGCAGACCTGTGCCCGTGGACACTGTGCCAACAGCTGTGAGAGGGGCGACACCACCACC
CTGTACAGCCAGGGCGGCCATGGGCACGATCCCAAGTCTGGCTTCCGCATCTATTTCTGC
CAGATCCCCTGCCTGAACGGAGGCCGCTGCATCGGCAGGGACGAATGCTGGTGCCCCGCC
AACTCCACCGGGAAGTTCTGCCACCTGCCTATCCCGCAGCCGGACAGGGAGCCTCCAGGG
AGGGGGTCCCGCCCCAGGGCCTTGCTGGAAGCCCCACTGAAGCAGTCCACTTTCACACTG
CCGCTCTCCAACCAGCTGGCCTCCGTGAACCCCTCCCTGGTGAAGGTGCACATTCACCAC
CCACCCGAGGCCTCAGTGCAGATCCACCAGGTGGCCCAGGTGCGGGGCGGGGTGGAGGAG
GCCCTAGTGGAGAACAGCGTGGAGACCAGACCCCCGCCCTGGCTGCCTGCCAGCCCTGGC
CACAGCCTCTGGGACAGCAACAACATCCCTGCTCGGTCTGGAGAGCCCCCTCGGCCACTG
CCCCCAGCAGCACCCAGGCCTCGAGGACTGCTGGGCCGGTGTTACCTGAACACTGTGAAC
GGACAGTGTGCCAACCCTCTGCTGGAGCTGACTACCCAGGAGGACTGCTGTGGCAGTGTG
GGAGCCTTCTGGGGGGTGACTTTGTGTGCCCCATGCCCACCCAGACCAGCCTCCCCGGTG
ATTGAGAATGGCCAGCTGGAGTGTCCTCAGGGGTACAAGAGACTGAACCTCACTCACTGC
CAAGATATCAACGAGTGCTTGACCCTGGGCCTGTGCAAGGACGCGGAGTGTGTGAATACC
AGGGGCAGCTACCTGTGCACATGCAGACCTGGCCTCATGCTGGATCCATCGCGGAGCCGC
TGTGTGTCGGACAAGGCAATCTCCATGCTGCAGGGACTGTGCTACCGGTCGCTGGGGCCC
GGCACCTGCACCCTGCCTTTGGCCCAGCGGATCACCAAGCAGATATGCTGCTGCAGCCGC
GTGGGCAAAGCATGGGGCAGCGAGTGTGAGAAATGCCCTCTGCCTGGCACAGAGGCCTTC
AGAGAGATCTGCCCTGCCGGCCACGGCTACACCTACGCGAGCTCCGACATCCGCCTGTCC
ATGAGGAAAGCCGAGGAGGAGGAACTGGCAAGGCCCCCAAGGGAGCAAGGGCAGAGGAGC
AGCGGGGCACTGCCCGGGCCAGCAGAGAGGCAGCCCCTCCGGGTCGTCACGGACACCTGG
CTTGAGGCCGGGACCATCCCTGACAAGGGTGACTCTCAGGCTGGCCAGGTCACGACCAGT
GTCACTCATGCACCTGCCTGGGTCACAGGGAATGCCACAACCCCACCAATGCCTGAACAG
GGGATTGCAGAGATACAGGAAGAACAAGTGACCCCCTCCACCGATGTGCTGGTGACCCTG
AGCACCCCAGGCATTGACAGATGCGCTGCTGGAGCCACCAACGTCTGTGGCCCTGGAACC
TGCGTGAACCTCCCCGATGGATACAGATGTGTCTGCAGCCCTGGCTACCAGCTGCACCCC
AGCCAGGCCTACTGCACAGATGACAACGAGTGTCTGAGGGACCCCTGCAAGGGAAAAGGG
CGCTGCATCAACCGCGTGGGGTCCTACTCCTGCTTCTGCTACCCTGGCTACACTCTGGCC
ACCTCAGGGGCGACACAGGAGTGTCAAGATATCAATGAGTGTGAGCAGCCAGGGGTGTGC
AGCGGGGGGCAGTGCACCAACACCGAGGGCTCGTACCACTGCGAGTGTGATCAGGGCTAC
ATCATGGTCAGGAAAGGACACTGCCAAGATATCAACGAATGCCGTCACCCCGGTACCTGC
CCTGATGGGAGATGCGTCAATTCCCCTGGCTCCTACACTTGTCTGGCCTGTGAGGAGGGC
TACCGGGGCCAGAGTGGGAGCTGTGTAGATGTGAATGAGTGTCTGACTCCCGGGGTCTGT
GCCCATGGAAAGTGCACCAACCTAGAAGGCTCCTTCAGATGCTCTTGTGAGCAGGGCTAT
GAGGTCACCTCAGATGAGAAGGGCTGCCAAGATGTGGATGAGTGTGCCAGCCGGGCCTCA
TGCCCCACAGGCCTCTGCCTCAACACGGAGGGCTCCTTCGCCTGCTCTGCCTGTGAGAAC
GGGTACTGGGTGAATGAAGACGGCACTGCCTGTGAAGACCTAGATGAGTGTGCCTTCCCG
GGAGTCTGCCCCTCCGGAGTCTGCACCAACACGGCTGGCTCCTTCTCCTGCAAGGACTGC
GATGGGGGCTACCGGCCCAGCCCCCTGGGTGACTCCTGTGAAGATGTGGATGAATGTGAA
GACCCCCAGAGCAGCTGCCTGGGAGGCGAGTGCAAGAACACTGTGGGCTCCTACCAGTGC
CTCTGTCCCCAGGGCTTCCAGCTGGCCAATGGCACCGTGTGTGAGGATGTGAATGAGTGC
ATGGGGGAGGAGCACTGCGCACCACACGGCGAGTGCCTCAACAGCCACGGGTCTTTCTTC
TGTCTGTGCGCGCCTGGCTTCGTCAGCGCAGAGGGGGGCACCAGCTGCCAGGATGTGGAC
GAGTGTGCCACCACAGACCCGTGTGTGGGAGGGCACTGTGTCAACACCGAGGGCTCCTTC
AACTGTCTATGTGAGACTGGCTTCCAGCCCTCCCCAGAGAGTGGAGAGTGTGTGGATATT
GACGAGTGTGAGGACTATGGAGACCCGGTGTGTGGCACCTGGAAGTGTGAAAACAGCCCT
GGCTCCTACCGCTGTGTTCTGGGCTGCCAGCCTGGCTTCCACATGGCCCCGAACGGAGAC
TGCATTGACATAGACGAGTGCGCCAACGACACCATGTGTGGCAGCCACGGCTTCTGTGAC
AACACTGATGGCTCCTTCCGCTGCCTCTGTGACCAGGGCTTCGAGATCTCTCCCTCAGGC
TGGGACTGTGTGGATGTGAACGAGTGTGAGCTTATGCTGGCGGTATGTGGGGCCGCGCTC
TGTGAGAACGTGGAGGGCTCCTTCCTGTGCCTCTGTGCCAGTGACCTGGAGGAGTACGAT
GCCCAGGAGGGGCACTGCCGCCCACGGGGGGCTGGAGGTCAGAGTATGTCTGAGGCCCCA
ACGGGGGACCATGCCCCGGCCCCCACCCGCATGGACTGCTACTCCGGGCAGAAGGGCCAT
GCGCCCTGCTCCAGTGTCCTGGGCCGGAACACCACACAGGCTGAATGCTGCTGCACCCAG
GGCGCTAGCTGGGGAGATGCCTGTGACCTCTGCCCGTCTGAGGACTCAGCTGAATTCAGC
GAGATCTGCCCTAGTGGAAAAGGCTACATTCCTGTGGAAGGAGCCTGGACGTTTGGACAG
ACCATGTACACAGATGCGGATGAGTGTGTGATATTCGGGCCTGGTCTCTGCCCGAACGGC
CGGTGCCTCAACACCGTGCCTGGTTATGTCTGCCTGTGCAATCCCGGCTTCCACTACGAT
GCTTCCCACAAGAAGTGTGAGGATCACGATGAGTGCCAGGACCTGGCCTGTGAGAATGGC
GAGTGCGTCAACACGGAGGGCTCCTTCCACTGCTTCTGCAGCCCCCCGCTCACCCTGGAC
CTCAGCCAGCAGCGCTGCATGAACAGCACCAGCAGCACGGAGGACCTCCCTGACCACGAC
ATCCACATGGACATCTGCTGGAAAAAAGTCACCAATGATGTGTGCAGCGAACCCCTGCGT
GGGCACCGCACCACCTACACGGAATGCTGCTGCCAGGACGGCGAGGCCTGGAGCCAGCAG
TGTGCTCTGTGTCCCCCGAGGAGCTCTGAGGTCTATGCTCAGCTGTGCAACGTGGCTCGC
ATTGAGGCAGAGCGGGAGGCCGGGGTCCACTTCCGGCCAGGCTATGAGTATGGCCCCGGG
CCCGATGACCTGCACTACAGCATCTATGGCCCAGATGGGGCCCCCTTCTACAACTACCTG
GGCCCCGAGGACACCGTCCCTGATCCTGCCTTCCCCAACACAGCCGGTCACTCAGCGGAC
CGCACACCCATCCTTGAGTCTCCTTTGCAGCCCTCAGAACTCCAGCCCCACTACGTGGCC
AGCCATCCAGAGCCCCCAGCCGGCTTCGAAGGGCTTCAGGCGGAGGAGTGCGGCATCCTG
AACGGCTGTGAGAATGGCCGCTGTGTGCGCGTGCGGGAGGGCTACACCTGTGACTGTTTT
GAGGGCTTCCAGCTGGATGCGGCCCACATGGCCTGCGTAGATGTGAATGAGTGTGATGAC
TTGAACGGGCCTGCTGTGCTCTGTGTCCATGGTTACTGCGAGAACACAGAGGGCTCCTAC
CGCTGCCACTGCTCCCCGGGATATGTGGCTGAGGCAGGGCCCCCCCACTGCACTGCCAAG
GAG
Protein sequence predicted based on nucleotide sequence
MRPRTKARSPGRALRNPWRGFLPLTLALFVGAGHAQRDPVGRYEPAGGDANRLRRPGGSY PAAAAAKVYSLFREQDAPVAGLQPVERAQPGWGSPRRPTEAEARRPSRAQQSRRVQPPAQ TRRSTPLGQQQPAPRTRAAPALPRLGTPQRSGAAPPTPPRGRLTGRNVCGGQCCPGWTTA NSTNHCIKPVCEPPCQNRGSCSRPQLCVCRSGFRGARCEEVIPDEEFDPQNSRLAPRRWA ERSPNLRRSSAAGEGTLARAQPPAPQSPPAPQSPPAGTLSGLSQTHPSQQHVGLSRTVRL HPTATASSQLSSNALPPGPGLEQRDGTQQAVPLEHPSSPWGLNLTEKIKKIKIVFTPTIC KQTCARGHCANSCERGDTTTLYSQGGHGHDPKSGFRIYFCQIPCLNGGRCIGRDECWCPA NSTGKFCHLPIPQPDREPPGRGSRPRALLEAPLKQSTFTLPLSNQLASVNPSLVKVHIHH PPEASVQIHQVAQVRGGVEEALVENSVETRPPPWLPASPGHSLWDSNNIPARSGEPPRPL PPAAPRPRGLLGRCYLNTVNGQCANPLLELTTQEDCCGSVGAFWGVTLCAPCPPRPASPV IENGQLECPQGYKRLNLTHCQDINECLTLGLCKDAECVNTRGSYLCTCRPGLMLDPSRSR CVSDKAISMLQGLCYRSLGPGTCTLPLAQRITKQICCCSRVGKAWGSECEKCPLPGTEAF REICPAGHGYTYASSDIRLSMRKAEEEELARPPREQGQRSSGALPGPAERQPLRVVTDTW LEAGTIPDKGDSQAGQVTTSVTHAPAWVTGNATTPPMPEQGIAEIQEEQVTPSTDVLVTL STPGIDRCAAGATNVCGPGTCVNLPDGYRCVCSPGYQLHPSQAYCTDDNECLRDPCKGKG RCINRVGSYSCFCYPGYTLATSGATQECQDINECEQPGVCSGGQCTNTEGSYHCECDQGY IMVRKGHCQDINECRHPGTCPDGRCVNSPGSYTCLACEEGYRGQSGSCVDVNECLTPGVC AHGKCTNLEGSFRCSCEQGYEVTSDEKGCQDVDECASRASCPTGLCLNTEGSFACSACEN GYWVNEDGTACEDLDECAFPGVCPSGVCTNTAGSFSCKDCDGGYRPSPLGDSCEDVDECE DPQSSCLGGECKNTVGSYQCLCPQGFQLANGTVCEDVNECMGEEHCAPHGECLNSHGSFF CLCAPGFVSAEGGTSCQDVDECATTDPCVGGHCVNTEGSFNCLCETGFQPSPESGECVDI DECEDYGDPVCGTWKCENSPGSYRCVLGCQPGFHMAPNGDCIDIDECANDTMCGSHGFCD NTDGSFRCLCDQGFEISPSGWDCVDVNECELMLAVCGAALCENVEGSFLCLCASDLEEYD AQEGHCRPRGAGGQSMSEAPTGDHAPAPTRMDCYSGQKGHAPCSSVLGRNTTQAECCCTQ GASWGDACDLCPSEDSAEFSEICPSGKGYIPVEGAWTFGQTMYTDADECVIFGPGLCPNG RCLNTVPGYVCLCNPGFHYDASHKKCEDHDECQDLACENGECVNTEGSFHCFCSPPLTLD LSQQRCMNSTSSTEDLPDHDIHMDICWKKVTNDVCSEPLRGHRTTYTECCCQDGEAWSQQ CALCPPRSSEVYAQLCNVARIEAEREAGVHFRPGYEYGPGPDDLHYSIYGPDGAPFYNYL GPEDTVPDPAFPNTAGHSADRTPILESPLQPSELQPHYVASHPEPPAGFEGLQAEECGIL NGCENGRCVRVREGYTCDCFEGFQLDAAHMACVDVNECDDLNGPAVLCVHGYCENTEGSY RCHCSPGYVAEAGPPHCTAKE
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223
Synonyms:
pDONR223, pENTR223-XnCDSYn
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp):
2815
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Mark Vidal
Publications:
PMID:
15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
bacterial origin of replication
2220
2753
gene fragment
pas
5p stem of pBR primose assembly site
63
73
gene fragment
pas prime
3p stem of pBR pas(L)
105
116
recombination site
attL1
attL1 recombination site
570
665
recombination site
attL2
attL2 recombination site
793
696
selectable marker
SpnR
spectinomycin resistance gene
1062
2072
trxn termination sequence
T1-T2
rrnBT1 and T2 terminators
510
224
