DNASU Plasmid | TLE4 (Homo sapiens) in pSpeedET (bacterial expression vector)

Explanation of Terms

Gene:

Gene Symbol: TLE4

Gene Name: transducin-like enhancer of split 4 (E(sp1) homolog, Drosophila)

Original Clone ID:

85498

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pSpeedET

Format:

FUSION

Source:

Joint Center for Structural Genomics

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

PSI:Biology
Joint Center for Structural Genomics

HsCD00627124

Price:

AVAILABLE

No restriction
Special MTA: PSI

Insert Sequence
Protein Sequence

Insert sequence: 2415nts

Open reading frame: 1 to 2415

ATGATTCGCGACCTGAGCAAGATGTACCCGCAGACCAGACACCCGGCACCGCATCAGCCT
GCTCAACCCTTTAAATTTACAATTTCCGAATCCTGTGATCGGATTAAGGAAGAGTTTCAG
TTTTTACAGGCCCAATACCACAGTCTGAAGCTGGAATGTGAGAAACTCGCCAGTGAGAAG
ACAGAGATGCAGCGGCATTATGTCATGTATTATGAAATGTCCTATGGGTTGAATATAGAA
ATGCACAAGCAGGCAGAGATTGTCAAGAGGCTGAATGCTATCTGTGCACAAGTCATTCCT
TTCCTGTCCCAAGAGCACCAGCAACAAGTGGTGCAGGCTGTGGAACGGGCCAAGCAGGTG
ACCATGGCAGAACTGAACGCCATCATTGGGCAACAACTCCAGGCCCAGCATTTATCACAT
GGACATGGTCTCCCCGTACCTCTGACTCCACACCCTTCAGGGCTCCAGCCCCCTGCCATT
CCACCCATCGGTAGCAGTGCCGGGCTTCTGGCCCTCTCCAGTGCTCTAGGAGGTCAGTCC
CATCTTCCAATTAAAGATGAGAAGAAGCACCATGACAATGATCACCAAAGAGACAGAGAC
TCCATCAAGAGCTCTTCAGTATCCCCATCAGCCAGTTTCCGAGGTGCTGAGAAGCACAGA
AACTCCGCAGACTACTCCTCAGAGAGCAAAAAGCAGAAAACTGAAGAAAAGGAAATTGCA
GCTCGTTATGACAGCGATGGTGAGAAAAGTGATGACAACTTGGTGGTTGACGTTTCCAAT
GAGGATCCATCTTCCCCTCGAGGGAGCCCAGCACATTCCCCCAGAGAGAATGGCCTAGAC
AAGACACGCCTACTCAAGAAAGATGCCCCGATTAGTCCAGCCTCTATTGCATCTTCCAGC
AGTACTCCCTCCTCCAAATCCAAAGAACTTAGCCTTAAGAGGGATATGGGGAAATTGAGT
GAAACACGTCTTAGCGAAGATGAACAATGCACATTGGGGTTACAGAGATGGTTTTGTCGC
CTGTGGTTTATGAATGAAAAATCTACTACTCCCGTCTCAAAGTCCAATACCCCTACTCCA
CGAACTGATGCGCCCACCCCAGGCAGTAACTCTACTCCCGGATTGAGGCCTGTACCTGGA
AAACCACCAGGAGTTGACCCTTTGGCCTCAAGCCTAAGGACCCCAATGGCAGTACCTTGT
CCATATCCAACTCCATTTGGGATTGTGCCCCATGCTGGAATGAACGGAGAGCTGACCAGC
CCCGGAGCGGCCTACGCTGGGCTCCACAACATCTCCCCTCAGATGAGCGCAGCTGCTGCC
GCCGCCGCTGCTGCTGCTGCCTATGGGAGATCACCAGTGGTGGGATTTGATCCACACCAT
CACATGCGTGTGCCAGCAATACCTCCAAACCTGACAGGCATTCCAGGAGGAAAACCAGCA
TACTCCTTCCATGTTAGCGCAGATGGTCAGATGCAGCCTGTCCCTTTTCCACCCGACGCC
CTCATCGGACCTGGAATCCCCCGGCATGCTCGCCAGATCAACACCCTCAACCACGGGGAG
GTGGTGTGCGCGGTGACCATCAGCAACCCCACGAGACACGTGTACACGGGTGGGAAGGGC
TGCGTCAAGGTCTGGGACATCAGCCACCCAGGCAATAAGAGTCCTGTCTCCCAGCTCGAC
TGTCTGAACAGGGATAACTACATCCGTTCCTGCAGATTGCTCCCTGATGGTCGCACCCTA
ATTGTTGGAGGGGAAGCCAGTACTTTGTCCATTTGGGACCTGGCGGCTCCAACCCCACGC
ATCAAGGCAGAGCTGACATCCTCGGCCCCCGCCTGCTATGCCCTGGCCATCAGCCCCGAT
TCCAAGGTCTGCTTCTCATGCTGCAGCGACGGCAACATCGCTGTGTGGGATCTGCACAAC
CAGACCTTGGTGAGGCAATTCCAGGGCCACACAGATGGAGCCAGCTGTATTGACATTTCT
AATGATGGCACCAAGCTCTGGACAGGTGGTTTGGACAACACGGTCAGGTCCTGGGACCTG
CGCGAGGGGCGGCAGCTGCAGCAGCACGACTTCACCTCCCAGATCTTTTCTCTGGGCTAC
TGCCCAACTGGAGAGTGGCTTGCAGTGGGGATGGAGAACAGCAATGTGGAAGTTTTGCAT
GTCACCAAGCCAGACAAATACCAACTACATCTTCATGAGAGCTGTGTGCTGTCGCTCAAG
TTTGCCCATTGTGGCAAATGGTTTGTAAGCACTGGAAAGGACAACCTTCTGAATGCCTGG
AGAACACCTTATGGGGCCAGTATATTCCAGTCCAAAGAATCCTCATCGGTGCTTAGCTGT
GACATCTCCGTGGACGACAAATACATTGTCACTGGCTCTGGGGATAAGAAGGCCACAGTT
TATGAAGTTATTTAT

Protein sequence predicted based on nucleotide sequence

MIRDLSKMYPQTRHPAPHQPAQPFKFTISESCDRIKEEFQFLQAQYHSLKLECEKLASEK
TEMQRHYVMYYEMSYGLNIEMHKQAEIVKRLNAICAQVIPFLSQEHQQQVVQAVERAKQV
TMAELNAIIGQQLQAQHLSHGHGLPVPLTPHPSGLQPPAIPPIGSSAGLLALSSALGGQS
HLPIKDEKKHHDNDHQRDRDSIKSSSVSPSASFRGAEKHRNSADYSSESKKQKTEEKEIA
ARYDSDGEKSDDNLVVDVSNEDPSSPRGSPAHSPRENGLDKTRLLKKDAPISPASIASSS
STPSSKSKELSLKRDMGKLSETRLSEDEQCTLGLQRWFCRLWFMNEKSTTPVSKSNTPTP
RTDAPTPGSNSTPGLRPVPGKPPGVDPLASSLRTPMAVPCPYPTPFGIVPHAGMNGELTS
PGAAYAGLHNISPQMSAAAAAAAAAAAYGRSPVVGFDPHHHMRVPAIPPNLTGIPGGKPA
YSFHVSADGQMQPVPFPPDALIGPGIPRHARQINTLNHGEVVCAVTISNPTRHVYTGGKG
CVKVWDISHPGNKSPVSQLDCLNRDNYIRSCRLLPDGRTLIVGGEASTLSIWDLAAPTPR
IKAELTSSAPACYALAISPDSKVCFSCCSDGNIAVWDLHNQTLVRQFQGHTDGASCIDIS
NDGTKLWTGGLDNTVRSWDLREGRQLQQHDFTSQIFSLGYCPTGEWLAVGMENSNVEVLH
VTKPDKYQLHLHESCVLSLKFAHCGKWFVSTGKDNLLNAWRTPYGASIFQSKESSSVLSC
DISVDDKYIVTGSGDKKATVYEVIY

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

NGS Verification

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: kanamycin

Bacterial Selection Condition: 50ug/mL kanamycin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.

Protein Expression Results:

ProteinExpressed: Not_Applicable

ProteinPurified: Not_Applicable

SolubleProtein: Not_Applicable

Find experimental protocols in 421222

Recommended expression in:

Not Applicable

Vector Name:

pSpeedET

Synonyms:

pSpeed-ET

Sequencing Primer:

Forward: pBAD forward

Reverse: alt_bad_rev

Description:

Bacterial expression vector, arabinose or T7 induced expression, adds N-terminal MGSDKIHHHHHHENLYFQG tag; kanamycin resistance; PIPE cloning.

Comments:

If this vector contains an insert, the ccdB death cassette will not be present.

Size (bp):

5464

Parent Vector:

None

Properties:

PIPE cloning, bacterial expression, in vitro transcription, inducible expression, with tag/fusion/marker

Author Name:

Joint Center for Structural Genomics

Publications:

PMID: 18004753

Title: Combining the polymerase incomplete primer extension method for cloning and mutagenesis with microscreening to accelerate structural genomics efforts.

Vector Map:

Vector Sequence:

Sequence:

AAGAAACCAATTGTCCATATTGCATCAGACATTGCCGTCACTGCGTCTTTTACTGGCTCTTCTCGCTAACCAAACCGGTAACCCCGCTTATTAAAAGCATTCTGTAACAAAGCGGGACCAAAGCCATGACAAAAACGCGTAACAAAAGTGTCTATAATCACGGCAGAAAAGTCCACATTGATTATTTGCACGGCGTCACACTTTGCTATGCCATAGCATTTTTATCCATAAGATTAGCGGATCCTACCTGACGCTTTTTATCGCAACTCTCTACTGTTTCTCCATACCCGTAATACGACTCACTATAGGGTTTTGTTTAACTTTAAGAAGGAGATATACATACCCATGGGATCTGATAAAATTCATCATCATCATCATCACGAAAACCTGTACTTCCAGGGCGCCGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATAATGTGTGGATTTTGAGTTAGGATCCGGCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGGAGAAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTGGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCCGGAATTCCGTATGGCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAACTGAAACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTACACATATATTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAAGGGTTTATTGAGAATATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGTTTCACCAGTTTTGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTTCACCATGGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATGCCGTTTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGATGAGTGGCAGGGCGGGGCGTAAACGCGTGGATCCGGCTTACTAAAAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGAATATATACTGATATGTATACCCGAAGTATGTCAAAAAGAGGTGTGCTATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCAATATCTCCGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGGCTGGTGAAATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACACGCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGGTGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTGGCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAACTTAATTAACTCTAGATAACTGAGAAGGAGATATACATACCCGGGTAAGTTTAAACGGTCTCCAGCTTGGCTGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTTTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGCCATATTCAACGGGAAACGTCTTGCTCTAGGCCGCGATTAAATTCCAACATGGATGCTGATTTATATGGGTATAAATGGGCTCGCGATAATGTCGGGCAATCAGGTGCGACAATCTATCGATTGTATGGGAAGCCCGATGCGCCAGAGTTGTTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGATGGTCAGACTAAACTGGCTGACGGAATTTATGCCTCTTCCGACCATCAAGCATTTTATCCGTACTCCTGATGATGCATGGTTACTCACCACTGCGATCCCCGGGAAAACAGCATTCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAATATTGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTTGCATTCGATTCCTGTTTGTAATTGTCCTTTTAACAGCGATCGCGTATTTCGTCTCGCTCAGGCGCAATCACGAATGAATAACGGTTTGGTTGATGCGAGTGATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCATAAACTTTTGCCATTCTCACCGGATTCAGTCGTCACTCATGGTGATTTCTCACTTGATAACCTTATTTTTGACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACGAGTCGGAATCGCAGACCGATACCAGGATCTTGCCATCCTATGGAACTGCCTCGGTGAGTTTTCTCCTTCATTACAGAAACGGCTTTTTCAAAAATATGGTATTGATAATCCTGATATGAATAAATTGCAGTTTCATTTGATGCTCGATGAGTTTTTCTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTGGGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGGCAGCAGATCAATTCGCGCGCGAAGGCGAAGCGGCATGCATAATGTGCCTGTCAAATGGACGAAGCAGGGATTCTGCAAACCCTATGCTACTCCGTCAAGCCGTCAATTGTCTGATTCGTTACCAATTATGACAACTTGACGGCTACATCATTCACTTTTTCTTCACAACCGGCACGGAACTCGCTCGGGCTGGCCCCGGTGCATTTTTTAAATACCCGCGAGAAATAGAGTTGATCGTCAAAACCAACATTGCGACCGACGGTGGCGATAGGCATCCGGGTGGTGCTCAAAAGCAGCTTCGCCTGGCTGATACGTTGGTCCTCGCGCCAGCTTAAGACGCTAATCCCTAACTGCTGGCGGAAAAGATGTGACAGACGCGACGGCGACAAGCAAACATGCTGTGCGACGCTGGCGATATCAAAATTGCTGTCTGCCAGGTGATCGCTGATGTACTGACAAGCCTCGCGTACCCGATTATCCATCGGTGGATGGAGCGACTCGTTAATCGCTTCCATGCGCCGCAGTAACAATTGCTCAAGCAGATTTATCGCCAGCAGCTCCGAATAGCGCCCTTCCCCTTGCCCGGCGTTAATGATTTGCCCAAACAGGTCGCTGAAATGCGGCTGGTGCGCTTCATCCGGGCGAAAGAACCCCGTATTGGCAAATATTGACGGCCAGTTAAGCCATTCATGCCAGTAGGCGCGCGGACGAAAGTAAACCCACTGGTGATACCATTCGCGAGCCTCCGGATGACGACCGTAGTGATGAATCTCTCCTGGCGGGAACAGCAAAATATCACCCGGTCGGCAAACAAATTCTCGTCCCTGATTTTTCACCACCCCCTGACCGCGAATGGTGAGATTGAGAATATAACCTTTCATTCCCAGCGGTCGGTCGATAAAAAAATCGAGATAACCGTTGGCCTCAATCGGCGTTAAACCCGCCACCAGATGGGCATTAAACGAGTATCCCGGCAGCAGGGGATCATTTTGCGCTTCAGCCATACTTTTCATACTCCCGCCATTCAGAG

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

ColE1-type origin of replication

3306

3988

inducible trxn activator protein gene

araC

araC coding sequence

4563

5438

negative selection marker

ccdB

ccdB death cassette gene (lost in with-insert form)

1596

1805

promoter

T7 promoter

290

310

promoter

araC

araC promoter

256

285

protease cleavage site

TEV

TEV protease cleavage site

382

402

selectable marker

CmR

chloramphenicol resistance gene (CmR) (lost in with-insert form)

502

1158

selectable marker

KanR

kanamycin resistance gene

2396

3208

tag

His

N-terminal 6xHis tag

364

381

trxn regulatory element

arabinose O1 operator

161

182

trxn regulatory element

arabinose O2 operator

trxn termination sequence

rrnB

rrnB T2 transcription terminator

2258

2284

Gene

Gene Symbol :

TLE4

Symbol Nomenclature :

TLE4

SYNONYM :

BCE-1; BCE1; E(spI); ESG; ESG4; GRG4

Designation :

B lymphocyte gene 1|enhancer of split groucho 4|transducin-like enhancer of split 4, homolog of Drosophila E(sp1)|transducin-like enhancer protein 4

Full Nomenclature :

transducin-like enhancer of split 4 (E(sp1) homolog, Drosophila)

GENEID :

7091

Locus Tag :

RP11-79D8.3

GI :

71052126

GenBank Accession :

BC059405

HGNC :

11840

MIM :

605132

Target GenBank:

BC059405

Pathways

NCI :

Regulation of nuclear beta catenin signaling and target gene transcription

Panther :

Wnt signaling pathway

Reactome :

deactivation of the beta-catenin transactivating complex

Reactome :

Disease

Reactome :

FBXW7 Mutants and NOTCH1 in Cancer

Reactome :

formation of the beta-catenin:TCF transactivating complex

Reactome :

NOTCH1 Intracellular Domain Regulates Transcription

Reactome :

Signal Transduction

Reactome :

Signaling by NOTCH

Reactome :

Signaling by NOTCH1

Reactome :

Signaling by NOTCH1 HD Domain Mutants in Cancer

Reactome :

Signaling by NOTCH1 HD+PEST Domain Mutants in Cancer

Reactome :

Signaling by NOTCH1 in Cancer

Reactome :

Signaling by NOTCH1 PEST Domain Mutants in Cancer

Reactome :

Signaling by NOTCH1 t(7;9)(NOTCH1:M1580_K2555) Translocation Mutant

Reactome :

Signaling by Wnt

Reactome :

TCF dependent signaling in response to WNT

Protein

Structural Annotation Wiki :

421222

Clone

Cloning Information :

622400

Original Clone ID :

85498

Experimental

TargetTrack - Experimental data :

421222

Organism

Species Specific ID:

7091

Genome

Chromosome :

Map Location :

9q21.31

Ensembl :

ENSG00000106829