Gene:
Gene Symbol:
UL19
Gene Name:
6 copies form hexons, 5 copies form pentons
Original Clone ID:
UL19
Keyword:
Full length: Yes; NT position: 1--4125; aa position: 1-1375
Species:
Human herpesvirus 1
Type:
cDNA
Vector Name:
pANT7_cGST
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
PMID: 26450993
Title: Immunoproteomic Profiling of Antiviral Antibodies in New-Onset Type 1 Diabetes Using Protein Arrays
Title: Immunoproteomic Profiling of Antiviral Antibodies in New-Onset Type 1 Diabetes Using Protein Arrays
Authors:
Center for Personalized Diagnostics
Arizona State University
Xiaofang Bian
Arizona State University
Xiaofang Bian
HhCD00631715
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 4122nts
Open reading frame: 1 to 4119
ATGGCCGCTCCCAACCGCGACCCTCCGGGATACCGGTATGCCGCGGCCATGGTGCCGACC
GGGTCCCTCCTTAGCACGATCGAGGTGGCGTCGCATCGACGCCTGTTTGATTTTTTTTCC
CGCGTGCGCTCCGATGCAAACAGCCTGTACGACGTCGAGTTCGACGCCCTGCTGGGGTCG
TATTGCAACACCCTGTCGCTCGTGCGCTTTCTGGAGCTCGGGTTGTCGGTGGCGTGCGTG
TGTACCAAGTTTCCGGAGCTGGCCTACATGAACGAGGGGCGCGTACAGTTCGAGGTCCAC
CAGCCGCTGATTGCCCGCGACGGCCCGCACCCCATCGAGCAACCCACACACAATTACATG
ACCAAGATTATCGACCGCCGGGCCCTGAACGCCGCCTTCAGCCTGGCCACCGAGGCCATC
GCCCTGCTCACGGGGGAGGCCCTGGACGGGACGGGTATCGGCGCGCATCGCCAGTTGCGC
GCCATCCAACAGCTCGCTCGCAACGTCCAGGCCGTCCTCGGGGCGTTTGAGCGCGGCACG
GCCGACCAGATGCTGCACGTGCTGCTGGAAAAGGCGCCGCCCCTGGCTTTGTTGTTGCCG
ATGCAACGATACCTGGACAACGGCCGCCTGGCCACCAGGGTGGCCCGGGCGACCCTGGTC
GCCGAGCTAAAGCGAAGCTTCTGCGAGACGAGCTTTTTCCTGGGCAAGGCGGGCCACCGC
CGGGAGGCCGTCGAGGCCTGGCTCGTGGACCTCACCACGGCCACGCAGCCCTCCGTGGCC
GTGCCCCGTCTGACGCATGCCGACACGCGCGGGCGGCCGGTCGACGGGGTGCTCGTCACC
ACCGCCCCGATCAAACAGCGCCTCCTGCAGTCCTTCCTGAAAGTGGAGGACACCGAAGCC
GACGTGCCGGTGACGTACGGCGAGATGGTCCTGAACGGGGCCAACCTGGTCACGGCGCTC
GTGATGGGCAAGGCCGTGCGAAGTCTGGACGACGTGGGCCGCCACCTGCTGGAGATGCAG
GAGGAGCAGCTCGACCTGAACCGGCAGACGCTGGACGAGCTCGAGAGCGCCCCCCAGACG
ACGCGCGTGCGCGCGGATCTGGTGTCCATCGGCGAGAAGCTGGTCTTTCTGGAGGCCCTG
GAGAAGCGCATCTACGCCGCCACCAACGTTCCCTACCCCCTGGTGGGCGCCATGGACCTG
ACGTTCGTCCTGCCCCTGGGCCTGTTCAATCCGGTCATGGAACGGTTTGCCGCGCACGCC
GGGGACCTAGTCCCCGCCCCCGGCCACCCGGATCCCCGCGCCTTCCCGCCCCGCCAGCTG
TTTTTTTGGGGGAAGGACCGCCAGGTGCTGCGCCTGTCTCTGGAACACGCGATCGGGACC
GTGTGCCACCCTTCGCTGATGAACGTTGACGCGGCGGTCGGGGGCCTTAACCGCGACCCC
GTCGAAGCCGCCAATCCGTACGGGGCGTACGTGGCGGCCCCGGCCGGCCCCGCCGCAGAC
ATGCAGCAGCTGTTTTTGAACGCCTGGGGGCAGCGCCTGGCCCACGGGCGGGTCCGATGG
GTCGCGGAAGGCCAGATGACCCCGGAGCAGTTTATGCAGCCCGACAACGCCAACCTGGCT
CTTGAGCTGCACCCCGCGTTCGACTTCTTTGTGGGGGTGGCCGACGTGGAGCTGCCGGGG
GGGGACGTTCCCCCGGCCGGCCCGGGGGAGATCCAGGCCACCTGGCGCGTGGTGAACGGC
AACTTGCCCCTGGCGCTATGTCCGGCGGCGTTCCGGGACGCCCGGGGCCTGGAGCTGGGG
GTGGGACGCCACGCCATGGCCCCCGCCACCATCGCCGCCGTTCGCGGGGCGTTCGACGAC
CGCAACTACCCGGCGGTGTTTTACCTGCTGCAGGCCGCCATACACGGCAGCGAGCACGTC
TTCTGCGCCCTGGCCCGGCTCGTGGTCCAGTGCATCACCAGCTACTGGAACAACACGCGG
TGCGCGGCGTTCGTGAACGACTACTCGCTGGTCTCGTACGTCGTGACCTACCTCGGGGGA
GACCTTCCCGAGGAGTGCATGGCCGTGTACCGGGACCTGGTGGCGCACGTCGAGGCCCTG
GCCCAGCTGGTTGATGACTTTACCCTGACCGGCCCGGAGCTGGGCGGGCAGGCGCAAGCC
GAGCTGAATCACCTAATGCGAGACCCGGCGCTGCTGCCACCCCTCGTGTGGGACTGTGAC
GCCCTGATGCGGCGCGCGGCCCTGGACCGCCATCGCGACTGCCGGGTTAGCGCGGGGGGC
CACGACCCCGTGTACGCGGCGGCATGTAACGTGGCGACCGCGGACTTCAACCGCAACGAC
GGCCAGCTGCTGCACAACACCCAGGCCCGAGCCGCGGACGCCGCGGATGACCGGCCGCAC
CGGGGGGCGGACTGGACCGTGCACCACAAGATTTACTACTACGTGATGGTGCCCGCCTTC
TCGCGGGGCCGCTGCTGCACCGCGGGGGTTCGCTTCGACCGCGTATACGCCACCCTGCAG
AACATGGTGGTCCCGGAGATCGCCCCCGGCGAGGAGTGCCCCAGCGACCCCGTGACGGAC
CCCGCGCACCCCCTGCACCCGGCCAATCTGGTGGCCAACACGGTCAACGCCATGTTTCAC
AACGGGCGCGTGGTAGTGGACGGGCCCGCCATGCTCACGCTGCAGGTGCTGGCCCACAAC
ATGGCCGAACGCACAACGGCGCTGCTCTGCTCGGCGGCGCCCGACGCGGGCGCCAACACC
GCGTCCACCACCAACATGCGCATATTCGACGGGGCGTTGCACGCCGGAATCCTGCTGATG
GCCCCCCAGCATCTGGACCATACCATCCAAAATGGCGACTATTTTTACCCCCTCCCCGTC
CACGCGCTGTTCGCCGGGGCCGACCACGTGGCGAACGCGCCCAATTTCCCCCCGGCCCTG
CGCGACCTGTCGCGGCAGGTCCCCCTGGTCCCCCCGGCTCTGGGGGCCAACTACTTTTCG
TCGATCCGACAGCCCGTCGTGCAGCACGTCCGCGAGAGCGCGGCCGGGGAGAACGCGCTG
ACCTACGCGCTCATGGCGGGGTACTTCAAGATCAGTCCCGTGGCCTTGCATCATCAGCTC
AAGACGGGCCTCCATCCCGGGTTTGGGTTCACCGTCGTCCGACAGGACCGCTTTGTGACT
GAGAACGTGCTGTTCTCGGAGCGCGCGTCGGAGGCGTACTTCCTGGGCCAGCTCCAGGTG
GCCCGGCACGAAACTGGCGGGGGGGTAAACTTCACGCTCACCCAGCCGCGCGCGAACGTG
GACCTGGGCGTGGGCTACACCGCCGTCGTGGCCACGGCAACCGTCCGCAACCCCGTCACC
GACATGGGCAACCTTCCCCAAAACTTTTACCTGGGCCGCGGGGCTCCCCCTCTCCTGGAC
AACGCGGCAGCCGTGTACCTGCGGAACGCGGTCGTGGCGGGAAACCGCCTGGGGCCGGCC
CAGCCCGTCCCCGTGTTCGGGTGCGCCCAGGTGCCGCGGCGCGCAGGGATGGACCACGGC
CAGGACGCCGTGTGTGAGTTTATCGCCACCCCCGTGTCGACCGACGTCAACTACTTCCGC
CGGCCCTGCAACCCCCGGGGACGCGCCGCCGGGGGCGTTTACGCGGGGGACAAGGAGGGG
GATGTCACCGCCCTCATGTACGACCACGGCCAGAGCGACCCGTCCCGGGCCTTCGCGGCC
ACGGCCAACCCGTGGGCGTCGCAGCGATTTTCGTACGGGGACCTGCTCTATAACGGGGCC
TACCACCTCAACGGGGCCTCGCCGGTGCTCAGCCCCTGCTTTAAGTTCTTTACGTCGGCC
GACATCGCCGCCAAACATCGCTGCCTGGAGCGCCTGATCGTGGAGACGGGTTCGGCGGTG
TCCACGGCCACCGCCGCCAGCGACGTACAGTTTAAGCGCCCCCCGGGGTGCCGCGAACTC
GTGGAGGACCCGTGTGGCCTGTTTCAGGAGGCCTACCCGCTCACCTGCGCCAGCGACCCC
GCCCTCCTCCGCAGTGCCCGCAACGGGGAAGCCCACGCGCGGGAGACCCACTTCGCGCAG
TATCTCGTCTATGACGCATCCCCGCTCAAGGGACTGGCTCTG
Protein sequence predicted based on nucleotide sequence
MAAPNRDPPGYRYAAAMVPTGSLLSTIEVASHRRLFDFFSRVRSDANSLYDVEFDALLGS YCNTLSLVRFLELGLSVACVCTKFPELAYMNEGRVQFEVHQPLIARDGPHPIEQPTHNYM TKIIDRRALNAAFSLATEAIALLTGEALDGTGIGAHRQLRAIQQLARNVQAVLGAFERGT ADQMLHVLLEKAPPLALLLPMQRYLDNGRLATRVARATLVAELKRSFCETSFFLGKAGHR REAVEAWLVDLTTATQPSVAVPRLTHADTRGRPVDGVLVTTAPIKQRLLQSFLKVEDTEA DVPVTYGEMVLNGANLVTALVMGKAVRSLDDVGRHLLEMQEEQLDLNRQTLDELESAPQT TRVRADLVSIGEKLVFLEALEKRIYAATNVPYPLVGAMDLTFVLPLGLFNPVMERFAAHA GDLVPAPGHPDPRAFPPRQLFFWGKDRQVLRLSLEHAIGTVCHPSLMNVDAAVGGLNRDP VEAANPYGAYVAAPAGPAADMQQLFLNAWGQRLAHGRVRWVAEGQMTPEQFMQPDNANLA LELHPAFDFFVGVADVELPGGDVPPAGPGEIQATWRVVNGNLPLALCPAAFRDARGLELG VGRHAMAPATIAAVRGAFDDRNYPAVFYLLQAAIHGSEHVFCALARLVVQCITSYWNNTR CAAFVNDYSLVSYVVTYLGGDLPEECMAVYRDLVAHVEALAQLVDDFTLTGPELGGQAQA ELNHLMRDPALLPPLVWDCDALMRRAALDRHRDCRVSAGGHDPVYAAACNVATADFNRND GQLLHNTQARAADAADDRPHRGADWTVHHKIYYYVMVPAFSRGRCCTAGVRFDRVYATLQ NMVVPEIAPGEECPSDPVTDPAHPLHPANLVANTVNAMFHNGRVVVDGPAMLTLQVLAHN MAERTTALLCSAAPDAGANTASTTNMRIFDGALHAGILLMAPQHLDHTIQNGDYFYPLPV HALFAGADHVANAPNFPPALRDLSRQVPLVPPALGANYFSSIRQPVVQHVRESAAGENAL TYALMAGYFKISPVALHHQLKTGLHPGFGFTVVRQDRFVTENVLFSERASEAYFLGQLQV ARHETGGGVNFTLTQPRANVDLGVGYTAVVATATVRNPVTDMGNLPQNFYLGRGAPPLLD NAAAVYLRNAVVAGNRLGPAQPVPVFGCAQVPRRAGMDHGQDAVCEFIATPVSTDVNYFR RPCNPRGRAAGGVYAGDKEGDVTALMYDHGQSDPSRAFAATANPWASQRFSYGDLLYNGA YHLNGASPVLSPCFKFFTSADIAAKHRCLERLIVETGSAVSTATAASDVQFKRPPGCREL VEDPCGLFQEAYPLTCASDPALLRSARNGEAHARETHFAQYLVYDASPLKGLA
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pANT7_cGST
Synonyms:
pANT7-cGST
Description:
with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments:
One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp):
5964
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Joshua LaBaer
Al Lau
Niroshan Ramachandran
Al Lau
Niroshan Ramachandran
Publications:
PMID:
15232106
Title: Self-assembling protein microarrays
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 bacterial origin of replication
3931
3249
death cassette
ccdB
ccdB death cassette present in 'empty vector' form only (replace with gene of interest)
1876
2085
primer
NAP138
NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3?
2405
2384
primer
NAP150
Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3?
388
408
promoter
T7
T7 transcriptional start sequence
5940
5964
recombination site
attR1
AttR1 site for recombination in empty vector
549
565
recombination site
attR2
AttR2 site for recombination in empty vector
2236
2251
selectable marker
CmR
chloramphenicol resistance
782
1438
selectable marker
AmpR
ampicillin resistance gene
4688
4029
tag
GST
Glutathione transferase (GST) tag
2282
2950
