Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nGST_DC
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
Insert size was verirified by PCR
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
Arizona State University
Xiaobo Yu
Andrea Throop
Lerys del Moral
Joshua LaBaer
Arizona State University
Xiaobo Yu
Andrea Throop
Lerys del Moral
Joshua LaBaer
HsCD00632801
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 510nts
Open reading frame: 1 to 510
ATGACGGAATATAAGCTGGTGGTGGTGGGCGCCGGCGGTGTGGGCAAGAGTGCGCTGACC
ATCCAGCTGATCCAGAACCATTTTGTGGACGAATACGACCCCACTATAGAGGATTCCTAC
CGGAAGCAGGTGGTCATTGATGGGGAGACGTGCCTGTTGGACATCCTGGATACCGCCGGC
CAGGAGGAGTACAGCGCCATGCGGGACCAGTACATGCGCACCGGGGAGGGCTTCCTGTGT
GTGTTTGCCATCAACAACACCAAGTCTTTTGAGGACATCCACCAGTACAGGGAGCAGATC
AAACGGGTGAAGGACTCGGATGACGTGCCCATGGTGCTGGTGGGGAACAAGTGTGACCTG
GCTGCACGCACTGTGGAATCTCGGCAGGCTCAGGACCTCGCCCGAAGCTACGGCATCCCC
TACATCGAGACCTCGGCCAAGACCCGGCAGGGCAGCCGCTCTGGCTCTAGCTCCAGCTCC
GGGACCCTCTGGGACCCCCCGGGACCCATG
Protein sequence predicted based on nucleotide sequence
MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAG QEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHQYREQIKRVKDSDDVPMVLVGNKCDL AARTVESRQAQDLARSYGIPYIETSAKTRQGSRSGSSSSSGTLWDPPGPM
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nGST_DC
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable GST tag; ampicillin resistance; Gateway or Flexi cloning compatible
Comments:
Vector has tendency to recombine between repeat sequences that flank the att sites resulting in decreased LR efficiency
Size (bp):
5998
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Joshua LaBaer
Justin Saul
Ji Qiu
Fernanda Festa
Justin Saul
Ji Qiu
Fernanda Festa
Publications:
None
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 bacterial origin
3283
3965
bacterial origin
M13 Ori
M13 origin
5359
5814
death cassette
ccdB
ccdB death cassette (removed in clones containing an insert)
2479
2784
enhancer
CITE
CITE enhancer
17
515
primer
forward primer
GSTseqF forward sequencing primer gcaagtatatagcatggcct
1105
1124
promoter
T7
T7 promoter
2982
5998
protease cleavage site
TEV
TEV protease cleavage site (EDLYFQS)
1209
1229
recombination site
attR1
attR1 recombinational cloning site
1252
1269
recombination site
attR2
attR2 recombinational cloning site
2925
2942
reporter gene
LacZ
LacZ alpha
5819
5887
selectable marker
CmR
Chloramphenicol resistance (removed in plasmids containing an insert)
1478
2134
selectable marker
AmpR
Ampicillin resistance marker
4063
4772
ssDNA origin
f1
f1 origin
5491
5797
tag
GST
N-terminal TEV-cleavable GST tag
525
1190
trxn termination sequence
T7 term
T7 terminator
3054
3101
Gene
Gene Symbol :
HRAS
Symbol Nomenclature :
HRAS
SYNONYM :
C-BAS/HAS; C-H-RAS; C-HA-RAS1; CTLO; H-RASIDX; HAMSV; HRAS1; K-RAS; N-RAS; RASH1
Designation :
GTP- and GDP-binding peptide B|GTPase HRas|H-Ras-1|Ha-Ras1 proto-oncoprotein|Ras family small GTP binding protein H-Ras|c-has/bas p21 protein|c-ras-Ki-2 activated oncogene|p19 H-RasIDX protein|p21ras|transformation gene: oncogene HAMSV|transforming protein p21|v-Ha-ras Harvey rat sarcoma viral oncogene homolog
Full Nomenclature :
Harvey rat sarcoma viral oncogene homolog
GENEID :
HGNC :
MIM :
Vega :
Target GenBank:
None
Pathways
NCI :
a6b1 and a6b4 Integrin signaling
NCI :
BCR signaling pathway
NCI :
C-MYB transcription factor network
NCI :
CDC42 signaling events
NCI :
Class I PI3K signaling events
NCI :
CXCR3-mediated signaling events
NCI :
Downstream signaling in naïve CD8+ T cells
NCI :
EGF receptor (ErbB1) signaling pathway
NCI :
EGFR-dependent Endothelin signaling events
NCI :
Endothelins
NCI :
EPHB forward signaling
NCI :
EPO signaling pathway
NCI :
ErbB1 downstream signaling
NCI :
ErbB2/ErbB3 signaling events
NCI :
Fc-epsilon receptor I signaling in mast cells
NCI :
GMCSF-mediated signaling events
NCI :
IGF1 pathway
NCI :
IL2-mediated signaling events
NCI :
Insulin Pathway
NCI :
Internalization of ErbB1
NCI :
LPA receptor mediated events
NCI :
mTOR signaling pathway
NCI :
Neurotrophic factor-mediated Trk receptor signaling
NCI :
Nongenotropic Androgen signaling
NCI :
PDGFR-beta signaling pathway
NCI :
Plasma membrane estrogen receptor signaling
NCI :
Posttranslational regulation of adherens junction stability and dissassembly
NCI :
Ras signaling in the CD4+ TCR pathway
NCI :
Regulation of Ras family activation
NCI :
SHP2 signaling
NCI :
Signaling events mediated by Hepatocyte Growth Factor Receptor (c-Met)
NCI :
Signaling events mediated by Stem cell factor receptor (c-Kit)
NCI :
Signaling events regulated by Ret tyrosine kinase
NCI :
Syndecan-2-mediated signaling events
NCI :
TCR signaling in naïve CD4+ T cells
NCI :
TCR signaling in naïve CD8+ T cells
NCI :
Trk receptor signaling mediated by PI3K and PLC-gamma
NCI :
Trk receptor signaling mediated by the MAPK pathway
Panther :
Angiogenesis
Panther :
B cell activation
Panther :
EGF receptor signaling pathway
Panther :
FGF signaling pathway
Panther :
Integrin signalling pathway
Panther :
p53 pathway feedback loops 2
Panther :
PDGF signaling pathway
Panther :
Ras Pathway
Panther :
T cell activation
Panther :
TGF-beta signaling pathway
Panther :
VEGF signaling pathway
Reactome :
Activation of NMDA receptor upon glutamate binding and postsynaptic events
Reactome :
Activation of RAS in B cells
Reactome :
Adaptive Immune System
Reactome :
ARMS-mediated activation
Reactome :
Axon guidance
Reactome :
Cell surface interactions at the vascular wall
Reactome :
CREB phosphorylation through the activation of Ras
Reactome :
Cytokine Signaling in Immune system
Reactome :
DAP12 interactions
Reactome :
DAP12 signaling
Reactome :
Developmental Biology
Reactome :
Disease
Reactome :
Downstream signal transduction
Reactome :
Downstream signaling events of B Cell Receptor (BCR)
Reactome :
Downstream signaling of activated FGFR
Reactome :
EGFR Transactivation by Gastrin
Reactome :
Fc epsilon receptor (FCERI) signaling
Reactome :
FCERI mediated MAPK activation
Reactome :
Frs2-mediated activation
Reactome :
FRS2-mediated cascade
Reactome :
Gastrin-CREB signalling pathway via PKC and MAPK
Reactome :
GRB2 events in EGFR signaling
Reactome :
GRB2 events in ERBB2 signaling
Reactome :
Hemostasis
Reactome :
IGF1R signaling cascade
Reactome :
Immune System
Reactome :
Innate Immune System
Reactome :
Insulin receptor signalling cascade
Reactome :
Interleukin receptor SHC signaling
Reactome :
Interleukin-2 signaling
Reactome :
Interleukin-3, 5 and GM-CSF signaling
Reactome :
IRS-mediated signalling
Reactome :
IRS-related events
Reactome :
IRS-related events triggered by IGF1R
Reactome :
MEK activation
Reactome :
NCAM signaling for neurite out-growth
Reactome :
Neuronal System
Reactome :
Neurotransmitter Receptor Binding And Downstream Transmission In The Postsynaptic Cell
Reactome :
NGF signalling via TRKA from the plasma membrane
Reactome :
p38MAPK events
Reactome :
Post NMDA receptor activation events
Reactome :
Prolonged ERK activation events
Reactome :
RAF activation
Reactome :
RAF phosphorylates MEK
Reactome :
RAF/MAP kinase cascade
Reactome :
Ras activation uopn Ca2+ infux through NMDA receptor
Reactome :
SHC-mediated cascade
Reactome :
SHC-mediated signalling
Reactome :
SHC-related events
Reactome :
SHC-related events triggered by IGF1R
Reactome :
SHC1 events in EGFR signaling
Reactome :
SHC1 events in ERBB2 signaling
Reactome :
SHC1 events in ERBB4 signaling
Reactome :
Signal Transduction
Reactome :
Signaling by constitutively active EGFR
Reactome :
Signaling by EGFR
Reactome :
Signaling by EGFR in Cancer
Reactome :
Signaling by ERBB2
Reactome :
Signaling by ERBB4
Reactome :
Signaling by FGFR
Reactome :
Signaling by FGFR in disease
Reactome :
Signaling by FGFR mutants
Reactome :
Signaling by GPCR
Reactome :
Signaling by Insulin receptor
Reactome :
Signaling by Interleukins
Reactome :
Signaling by Leptin
Reactome :
Signaling by PDGF
Reactome :
Signaling by SCF-KIT
Reactome :
Signaling by the B Cell Receptor (BCR)
Reactome :
Signaling by Type 1 Insulin-like Growth Factor 1 Receptor (IGF1R)
Reactome :
Signalling by NGF
Reactome :
Signalling to ERKs
Reactome :
Signalling to p38 via RIT and RIN
Reactome :
Signalling to RAS
Reactome :
SOS-mediated signalling
Reactome :
Tie2 Signaling
Reactome :
Transmission across Chemical Synapses
WikiPathway :
Alpha 6 Beta 4 signaling pathway
WikiPathway :
B Cell Receptor Signaling Pathway
WikiPathway :
ErbB signaling pathway
WikiPathway :
G Protein Signaling Pathways
WikiPathway :
IL-2 Signaling pathway
WikiPathway :
IL-3 Signaling Pathway
WikiPathway :
Insulin Signaling
WikiPathway :
Integrin-mediated cell adhesion
WikiPathway :
Kit receptor signaling pathway
WikiPathway :
Leptin signaling pathway
WikiPathway :
MAPK Cascade
WikiPathway :
p38 MAPK Signaling Pathway
WikiPathway :
Prolactin Signaling Pathway
WikiPathway :
Senescence and Autophagy
WikiPathway :
Signaling of Hepatocyte Growth Factor Receptor
WikiPathway :
TCR Signaling Pathway
WikiPathway :
TGF Beta Signaling Pathway
WikiPathway :
TSH signaling pathway
Protein
HPRD :
01813
Clone
Cloning Information :
Organism
Species Specific ID:
Genome