Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
FLH233379.01X
Keyword:
None
Species:
Francisella tularensis subsp. tularensis SCHU S4
Type:
cDNA
Vector Name:
pDEST17
Format:
CLOSED
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
FtCD00063473
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 987nts
Open reading frame: 1 to 987
ATGCTATTATGGCCGGCTGAATGGGAGGAGCATAGTGCAACTTGGATGATCTGGCCAGCA
CGTATAGATATGTGGCCAAATATAACAAAAGCCTATGAGATATATGCTAAAGTTGCTAAC
ACTATCGCTAAATATGAGCCTGTAAATATGGTAGTAAATCAGCATCAGTTAGATATTGCA
AAAAAATATCTTGGAAAAAACATGACCCTGATAAGTGAAGCAGTAGATGATAGTTGGGCT
AGAGATATTATGCCAATTTTTTCATTTAAAGCAGATAAACTCATTGCAAATAATTTTGAT
TTTAACTGCTGGGGTAATAAGTTTTCACCTTTTGATAACGATAGAAGACTTAAAAATGAT
ATTGCAAAACAACAAAAATGGCAGGTTAATTCTTCTAAAATGATTTTAGAGGGTGGAGCA
GTACATTCAAATGGTCAAGGAGTTTTATTAACAACCAAGGAATGCTTACTAAATTTAAAC
CGTAATCCAAATATGCAAAAAGAGCAAATTGAAAGTGAGTTAATCAGTATTTTGGGAGTT
AAAAAAATTCTTTGGCTACCATATGGTGTAGCAGGTGATTTTGATACAGATGGTCGTGTT
GATAACGTTGCTTGCTTTGCCAATAAAAATACGATAATTATTCAAAGTTGTTATGATGAA
AATGATGAGAACTTTGCACGCCATCAAGCGAATATGACATATTTAGACAAATATGCCAGT
GAGTTTAATATAGTCGAAATACCTCAGCCTCGAGCACAATATTTCGCTGGTGAACGATTG
GCTTTATCTTACCTTAATTTTTATATTGTCAATAACGCTATAATTATGCCAGCATTTGGC
GATCCAAATGATGTAATAGCTTTTGAAATTCTACAGAAATGTTTTAAGGATCGTAGTATT
GAACAGCTTAATATTATTGACCTTGTAATTGGTGGAGGAGGAATTCACTGTATTACTATG
CAACAACCGGCTACAAAGGAGACTTAG
Protein sequence predicted based on nucleotide sequence
MLLWPAEWEEHSATWMIWPARIDMWPNITKAYEIYAKVANTIAKYEPVNMVVNQHQLDIA KKYLGKNMTLISEAVDDSWARDIMPIFSFKADKLIANNFDFNCWGNKFSPFDNDRRLKND IAKQQKWQVNSSKMILEGGAVHSNGQGVLLTTKECLLNLNRNPNMQKEQIESELISILGV KKILWLPYGVAGDFDTDGRVDNVACFANKNTIIIQSCYDENDENFARHQANMTYLDKYAS EFNIVEIPQPRAQYFAGERLALSYLNFYIVNNAIIMPAFGDPNDVIAFEILQKCFKDRSI EQLNIIDLVIGGGGIHCITMQQPATKET*
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Insert was fully sequenced in parent vector
Reference Sequence Annotations:
Start on reference sequence
1
End on reference sequence
987
Mutations:
None
Discrepancy:
t974c,I325T; t983c,I328T
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDEST17
Synonyms:
pDEST-17
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Bacterial expression vector with T7 promoter and ribosome binding site (RBS), adds N-term 6xHis tag; amp resistance; recombinational cloning.
Comments:
None
Size (bp):
6354
Parent Vector:
None
Properties:
Gateway, acceptor (destination), bacterial expression, in vitro transcription, recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
ATG start
ATG
initiation ATG (before 6xHis tag)
101
103
bacterial origin
pBR322 ori
pBR322 origin of replication
3575
4248
gene fragment
ROP
ROP ORF
4619
4810
negative selection marker
ccdB
ccdB death cassette ONLY in Non-Recombined (NO INSERT) form
1374
1679
promoter
T7
T7 promoter
21
40
promoter
bla
bla promoter (prokaryotic promoter from bla gene)
2471
2569
recombination site
attR1
attR1 recombination site
140
264
recombination site
attR2
attR2 recombination site
1720
1844
ribosome binding site
RBS
Ribosome binding site
86
92
selectable marker
CmR
chlorampenicol resistance gene ONLY In Non-Recombined (NO INSERT) form
373
1032
selectable marker
AmpR
ampicillin resistance gene (bla)
2570
3430
tag
His
6xHis tag (N-terminal to insert)
113
130
trxn termination sequence
T7 term
T7 transcription termination sequence
1855
1983
