Gene:
Gene Symbol:
YCH1
Gene Name:
Ych1p
Original Clone ID:
YST4096_1_148
Keyword:
None
Species:
Saccharomyces cerevisiae
Type:
cDNA
Vector Name:
p15Tv-LIC
Format:
FUSION
Source:
Midwest Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
University of Toronto
PSI:Biology
Midwest Center for Structural Genomics
PSI:Biology
Midwest Center for Structural Genomics
ScCD00651250
Price:
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Insert sequence: 444nts
Open reading frame: 1 to 444
ATGGACTCGTACTCAATAACAAACGTAAAATACCTGGATCCGACTGAATTGCATCGTTGG
ATGCAAGAAGGACACACTACTACGCTGAGGGAGCCTTTCCAGGTAGTGGATGTGCGAGGC
TCAGATTATATGGGGGGCCATATCAAGGACGGATGGCACTACGCCTATTCGCGCCTTAAG
CAGGATCCGGAGTACTTACGTGAGCTAAAGCACAGATTGTTGGAAAAGCAAGCGGACGGC
CGCGGAGCGCTGAACGTGATTTTCCATTGTATGTTATCGCAGCAGCGCGGACCGTCTGCA
GCGATGCTGCTGCTTCGGTCGCTTGACACGGCGGAACTTTCTCGCTGTCGTCTATGGGTG
TTGCGTGGGGGGTTCTCGCGCTGGCAATCCGTATACGGTGACGACGAGAGCGTTACGGCG
GGTTACCTACCCGATCTGTGGCGT
Protein sequence predicted based on nucleotide sequence
MDSYSITNVKYLDPTELHRWMQEGHTTTLREPFQVVDVRGSDYMGGHIKDGWHYAYSRLK QDPEYLRELKHRLLEKQADGRGALNVIFHCMLSQQRGPSAAMLLLRSLDTAELSRCRLWV LRGGFSRWQSVYGDDESVTAGYLPDLWR
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in MCSG-APC7727
Recommended expression in:
Not Applicable
Vector Name:
p15Tv-LIC
Synonyms:
''
Description:
Bacterial (E. coli) expression vector, adds His tag, TEV cleavage site; ampicillin resistance; recombinational cloning (InFusion, BseRI).
Comments:
None
Size (bp):
7746
Parent Vector:
None
Properties:
acceptor (destination), bacterial expression, recombinational cloning, with tag/fusion/marker
Author Name:
PSI
Midwest Center for Structural Genomics
Midwest Center for Structural Genomics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
bacterial origin of replication
1164
1846
negative selection marker
sacB
sacB cds
5901
7319
promoter
T7
T7 promoter
5230
5249
recombination site
LoxP
LoxP sequence
5407
5440
repressor binding site
LacO
LacO sequence region
5249
5271
repressor protein gene
lacI
lacI cds
3764
4843
selectable marker
AmpR
ampicillin resistance gene
407
1066
trxn termination sequence
T7 term
T7 terminator sequence
7488
7534
