Gene:
Gene Symbol:
HNF4A
Gene Name:
hepatocyte nuclear factor 4, alpha
Original Clone ID:
HR7522B-142-378-TEV.4
Keyword:
targeted domain
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pET15Nano6HT_NESG
Format:
CLOSED
Source:
Northeast Structural Genomics Consortium
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
Rutgers University
PSI:Biology
Northeast Structural Genomics Consortium
PSI:Biology
Northeast Structural Genomics Consortium
HsCD00663617
Price:
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AVAILABLE
No restriction
Special MTA: PSI/NESG
Special MTA: PSI/NESG
Insert sequence: 714nts
Open reading frame: 10 to 723
AGCCATATGTCAAGCTATGAGGACAGCAGCCTGCCCTCCATCAATGCGCTCCTGCAGGCG
GAGGTCCTGTCCCAACAGATCACCTCCCCCGTCTCCGGGATCAACGGCGACATTCGGGCG
AAGAAGATTGCCAGCATCGCAGATGTGTGTGAGTCCATGAAGGAGCAGCTGCTGGTTCTC
GTTGAGTGGGCCAAGTACATCCCAGCTTTCTGCGAGCTCCCCCTGGACGACCAGGTGGCC
CTGCTCAGAGCCCATGCTGGCGAGCACCTGCTGCTCGGAGCCACCAAGAGATCCATGGTG
TTCAAGGACGTGCTGCTCCTAGGCAATGACTACATTGTCCCTCGGCACTGCCCGGAGCTG
GCGGAGATGAGCCGGGTGTCCATACGCATCCTTGACGAGCTGGTGCTGCCCTTCCAGGAG
CTGCAGATCGATGACAATGAGTATGCCTACCTCAAAGCCATCATCTTCTTTGACCCAGAT
GCCAAGGGGCTGAGCGATCCAGGGAAGATCAAGCGGCTGCGTTCCCAGGTGCAGGTGAGC
TTGGAGGACTACACCAACGACCGCCAGTACGACTCGCGTGGCCGCTTTGGAGAGCTGCTG
CTGCTGCTGCCCACCTTGCAGAGCATCACCTGGCAGATGATCGAGCAGATCCAGTTCATC
AAGCTCTTTGGCATGGCCAAGATTGACAACCTGTTGCAGGAGATGCTGCTGGGAGGGTCC
TAG
Protein sequence predicted based on nucleotide sequence
SSYEDSSLPSINALLQAEVLSQQITSPVSGINGDIRAKKIASIADVCESMKEQLLVLVEW AKYIPAFCELPLDDQVALLRAHAGEHLLLGATKRSMVFKDVLLLGNDYIVPRHCPELAEM SRVSIRILDELVLPFQELQIDDNEYAYLKAIIFFDPDAKGLSDPGKIKRLRSQVQVSLED YTNDRQYDSRGRFGELLLLLPTLQSITWQMIEQIQFIKLFGMAKIDNLLQEMLLGGS*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
g65a,R22Q;t545c,I182T;
t561c,Y187Y;c660t,F220F
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in HR7522B
Recommended expression in:
Not Applicable
Vector Name:
pET15Nano6HT_NESG
Synonyms:
''
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Bacterial expression withT7 lac promoter, N-terminal Nano-tag, 6XHis-tag, TEV-cleavable; ampicillin resistance in bacteria; restriction enzyme cloning
Comments:
None
Size (bp):
5783
Parent Vector:
None
Properties:
bacterial expression, multiple cloning site, with tag/fusion/marker
Author Name:
Rutgers University
Gaetano T. Montleione
Thomas B. Acton
Stephen Anderson
Northeast Structural Genomics Consortium
PSI:Biology
Gaetano T. Montleione
Thomas B. Acton
Stephen Anderson
Northeast Structural Genomics Consortium
PSI:Biology
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3938
4620
promoter
T7
T7 promoter
535
554
protease cleavage site
TEV
TEV protease cleavage site
384
401
repressor binding site
lac operator
lac operator
513
535
repressor protein gene
LacI
LacI repressor
944
2023
selectable marker
AmpR
Ampicillin resistance
4718
5377
tag
His
N-terminal 6xHis tag
402
419
tag
Nano
N-termianl streptavidin-binding Nano tag
420
464
