DNASU Plasmid | PIP5K1C (H. sapiens )<br>In pET15_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:

Gene Symbol: PIP5K1C

Gene Name: phosphatidylinositol-4-phosphate 5-kinase, type I, gamma

Original Clone ID:

HR3657-66-658-14.9

Keyword:

targeted domain

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pET15_NESG

Format:

CLOSED

Source:

Northeast Structural Genomics Consortium

Description:

None

Comments:

None

Mutation / Discrepancy:

No / Yes

Publications:

None

Authors:

Rutgers University
PSI:Biology
Northeast Structural Genomics Consortium

HsCD00670499

Price:

AVAILABLE

No restriction
Special MTA: PSI/NESG

Insert Sequence
Protein Sequence

Insert sequence: 1785nts

Open reading frame: 51 to 1835

ACTTTAGAAGGAGATATACCATGGGCCATCACCATCACCATCACAGCCATATGTCCGGCG
AAACCACCTACAAGAAGACCACCTCCTCCACCCTGAAGGGTGCCATCCAGCTGGGCATCG
GCTACACCGTGGGCCACCTGAGCTCCAAGCCCGAACGTGACGTACTCATGCAGGACTTCT
ACGTGGTGGAGAGCATCTTCTTCCCCAGCGAAGGCAGCAACTTCACCCCCGCCCACCACT
TCCAGGACTTCCGCTTCAAGACCTATGCACCTGTCGCCTTCCGCTACTTCCGGGAGCTCT
TTGGGATCCGGCCAGATGATTACTTGTACTCCCTGTGCAATGAGCCGCTGATCGAGCTGT
CCAACCCGGGCGCCAGTGGCTCCCTCTTCAACGTCACCAGCGACGACGAGTTCATCATCA
AGACCGTCATGCACAAGGAGGCCGAGTTCCTGCAGAAGCTGCTCCCTGGCTACTACATGA
ACCTCAACCAGAACCCGCGGACGCTGCTGCCCAAGATCTATGGGCTGTACTGCGTGCAGT
CGGGGGGCAAGAACATCCGCGTCGTGGTCATGAACAACATCCTGCCCCGCGTGGTCAAGA
TGCACCTCAAGTTCGACCTCAAGGGCTCCACCTACAAGCGGCGCGCCAGCAAGAAGGAGA
AGGAGAAGAGCTTCCCCACCTACAAGGACCTGGACTTCATGCAGGACATGCCCGAGGGGC
TCCTGCTGGACGCCGACACCTTCAGCGCCCTGGTCAAGACGCTGCAGCGGGACTGCCTGG
TCCTGGAAAGTTTCAAGATCATGGACTACTGTCTGCTGCTGGGTGTGCACAACATCGACC
AGCACGAGCGCGAGCGGCAGGCGCAGGGCGCCCAGAGCACCTCAGATGAGAAGCGGCCTG
TGGGCCAGAAGGCGCTCTACTCCACGGCCATGGAGTCCATCCAGGGTGGCGCCGCGCGCG
GGGAGGCCATCGAATCGGATGACACGATGGGCGGGATCCCCGCTGTGAACGGCCGCGGGG
AGCGGCTGCTGCTGCACATTGGCATCATCGACATCCTGCAGTCCTACAGGTTCATCAAGA
AACTGGAGCACACCTGGAAGGCCCTCGTCCACGATGGGGACACGGTGTCTGTCCACCGCC
CCAGCTTCTATGCCGAGCGCTTTTTCAAGTTCATGAGCAACACGGTCTTTCGGAAGAACT
CCTCCCTGAAGTCCTCGCCCTCCAAGAAGGGGCGCGGCGGAGCCTTGCTAGCTGTGAAAC
CGCTGGGGCCCACCGCTGCCTTCTCGGCCAGCCAGATCCCTAGCGAGCGGGAGGAGGCCC
AGTACGACCTGCGGGGGGCCCGCAGCTACCCCACGCTGGAGGACGAAGGCCGGCCCGACC
TCCTGCCCTGCACGCCACCTTCTTTCGAAGAAGCCACTACAGCCTCCATTGCCACGACTC
TGTCATCCACATCCCTCTCCATTCCTGAGCGGTCCCCCTCGGAGACGTCGGAGCAGCCGC
GGTACAGGCGGCGCACACAGTCGTCTGGACAGGGTGGCAGGCCGCAGGAGGAGCCACCCG
CGGAAGAGGATCTGCAGCAGATTACAGTGCAGGTGGAGCCTGCGTGCAGCGTGGAGATTG
TGGTCCCCAAAGAGGAGGACGCAGGGGTGGAGGCTTCTCCGGCCGGTGCCTCTGCTGCTG
TTGAAGTAGAAACTGCCAGCCAGGCCTCAGACGAGGAGGGCGCACCTGCCAGCCAGGCCT
CGGACGAGGAGGACGCGCCCGCCACCGACATCTACTTTCCCACCGATGAGAGGAGCTGGG
TGTACTCCCCGCTCCACTATAGCGCCCAGGCCTAG

Protein sequence predicted based on nucleotide sequence

MSGETTYKKTTSSTLKGAIQLGIGYTVGHLSSKPERDVLMQDFYVVESIFFPSEGSNFTP
AHHFQDFRFKTYAPVAFRYFRELFGIRPDDYLYSLCNEPLIELSNPGASGSLFNVTSDDE
FIIKTVMHKEAEFLQKLLPGYYMNLNQNPRTLLPKIYGLYCVQSGGKNIRVVVMNNILPR
VVKMHLKFDLKGSTYKRRASKKEKEKSFPTYKDLDFMQDMPEGLLLDADTFSALVKTLQR
DCLVLESFKIMDYCLLLGVHNIDQHERERQAQGAQSTSDEKRPVGQKALYSTAMESIQGG
AARGEAIESDDTMGGIPAVNGRGERLLLHIGIIDILQSYRFIKKLEHTWKALVHDGDTVS
VHRPSFYAERFFKFMSNTVFRKNSSLKSSPSKKGRGGALLAVKPLGPTAAFSASQIPSER
EEAQYDLRGARSYPTLEDEGRPDLLPCTPPSFEEATTASIATTLSSTSLSIPERSPSETS
EQPRYRRRTQSSGQGGRPQEEPPAEEDLQQITVQVEPACSVEIVVPKEEDAGVEASPAGA
SAAVEVETASQASDEEGAPASQASDEEDAPATDIYFPTDERSWVYSPLHYSAQA*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

c108t,R36R;g114a,V38V;

c172t,L58F;t340a,Y114N;

t466a,F156I;a760t,c762t,S254C;

c774t,G258G;c1080t,S360S;

a1484g,D495G;c1608t,S536S

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

ProteinPurified: Not_Tested

ProteinExpressed: Protein_Confirmed

SolubleProtein: Protein_Soluble

Find experimental protocols in HR3657

Recommended expression in:

Not Applicable

Vector Name:

pET15_NESG

Synonyms:

pET15, pET15-modified, pET15Cc, pET15_NESG

Sequencing Primer:

Forward: T7

Reverse: T7 terminator

Description:

Bacterial (E. coli) expression vector with T7 lac promoter, adds N-terminal 6x His tag; amp resistance; restriction enzyme cloning.

Comments:

Modified Novagen pET vector-common MCS

Size (bp):

5723

Parent Vector:

None

Properties:

bacterial expression, in vitro transcription, multiple cloning site, with tag/fusion/marker

Author Name:

Gaetano T. Montleione
Jessica Bearden
Northeast Structural Genomics Consortium

Publications:

PMID: 15808222

Title: Robotic cloning and Protein Production Platform of the Northeast Structural Genomics Consortium

Vector Map:

Vector Sequence:

Sequence:

TTCTCATGTTTGACAGCTTATCATCGATAAGCTAGCTTTAATGCGGTAGTTTATCACAGTTAAATTGCTAACGCAGTCAGGCACCGTGTATGAAATCTAACAATGCGCTCATCGTCATCCTCGGCACCGTCACCCTGGATGCTGTAGGCATAGGCTTGGTTATGCCGGTACTGCCGGGCCTCTTGCGGGATATCCGGATATAGTTCCTCCTTTCAGCAAAAAACCCCTCAAGACCCGTTTAGAGGCCCCAAGGGGTTATGCTAGTTATTGCTCAGCGGTGGCAGCAGCCAACTCAGCTTCCTTTCGGGCTTTGTTAGCAGCCGGATCTCGAGAAGCTTTGCGTCGACAGAGCTCGCAGGATCCGCAGAATTCGCCATATGGCTGTGATGGTGATGGTGATGGCCCATGGTATATCTCCTTCTTAAAGTTAAACAAAATTATTTCTAGAGGGGAATTGTTATCCGCTCACAATTCCCCTATAGTGAGTCGTATTAATTTCGCGGGATCGAGATCTCGATCCTCTACGCCGGACGCATCGTGGCCGGCATCACCGGCGCCACAGGTGCGGTTGCTGGCGCCTATATCGCCGACATCACCGATGGGGAAGATCGGGCTCGCCACTTCGGGCTCATGAGCGCTTGTTTCGGCGTGGGTATGGTGGCAGGCCCCGTGGCCGGGGGACTGTTGGGCGCCATCTCCTTGCATGCACCATTCCTTGCGGCGGCGGTGCTCAACGGCCTCAACCTACTACTGGGCTGCTTCCTAATGCAGGAGTCGCATAAGGGAGAGCGTCGAGATCCCGGACACCATCGAATGGCGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAATGTGAAACCAGTAACGTTATACGATGTCGCAGAGTATGCCGGTGTCTCTTATCAGACCGTTTCCCGCGTGGTGAACCAGGCCAGCCACGTTTCTGCGAAAACGCGGGAAAAAGTGGAAGCGGCGATGGCGGAGCTGAATTACATTCCCAACCGCGTGGCACAACAACTGGCGGGCAAACAGTCGTTGCTGATTGGCGTTGCCACCTCCAGTCTGGCCCTGCACGCGCCGTCGCAAATTGTCGCGGCGATTAAATCTCGCGCCGATCAACTGGGTGCCAGCGTGGTGGTGTCGATGGTAGAACGAAGCGGCGTCGAAGCCTGTAAAGCGGCGGTGCACAATCTTCTCGCGCAACGCGTCAGTGGGCTGATCATTAACTATCCGCTGGATGACCAGGATGCCATTGCTGTGGAAGCTGCCTGCACTAATGTTCCGGCGTTATTTCTTGATGTCTCTGACCAGACACCCATCAACAGTATTATTTTCTCCCATGAAGACGGTACGCGACTGGGCGTGGAGCATCTGGTCGCATTGGGTCACCAGCAAATCGCGCTGTTAGCGGGCCCATTAAGTTCTGTCTCGGCGCGTCTGCGTCTGGCTGGCTGGCATAAATATCTCACTCGCAATCAAATTCAGCCGATAGCGGAACGGGAAGGCGACTGGAGTGCCATGTCCGGTTTTCAACAAACCATGCAAATGCTGAATGAGGGCATCGTTCCCACTGCGATGCTGGTTGCCAACGATCAGATGGCGCTGGGCGCAATGCGCGCCATTACCGAGTCCGGGCTGCGCGTTGGTGCGGATATCTCGGTAGTGGGATACGACGATACCGAAGACAGCTCATGTTATATCCCGCCGTTAACCACCATCAAACAGGATTTTCGCCTGCTGGGGCAAACCAGCGTGGACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGGTGAAGGGCAATCAGCTGTTGCCCGTCTCACTGGTGAAAAGAAAAACCACCCTGGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTAAGTTAGCTCACTCATTAGGCACCGGGATCTCGACCGATGCCCTTGAGAGCCTTCAACCCAGTCAGCTCCTTCCGGTGGGCGCGGGGCATGACTATCGTCGCCGCACTTATGACTGTCTTCTTTATCATGCAACTCGTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGCGCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCCTTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATGGCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTCCCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTGTCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACCAGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGCCTCGGCGAGCACATGGAACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGTCGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACGGATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAACCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCATCTCGGGCAGCGTTGGGTCCTGGCCACGGGTGCGCATGATCGTGCTCCTGTCGTTGAGGACCCGGCTAGGCTGGCGGGGTTGCCTTACTGGTTAGCAGAATGAATCACCGATACGCGAGCGAACGTGAAGCGACTGCTGCTGCAAAACGTCTGCGACCTGAGCAACAACATGAATGGTCTTCGGTTTCCGTGTTTCGTAAAGTCTGGAAACGCGGAAGTCAGCGCCCTGCACCATTATGTTCCGGATCTGCATCGCAGGATGCTGCTGGCTACCCTGTGGAACACCTACATCTGTATTAACGAAGCGCTGGCATTGACCCTGAGTGATTTTTCTCTGGTCCCGCCGCATCCATACCGCCAGTTGTTTACCCTCACAACGTTCCAGTAACCGGGCATGTTCATCATCAGTAACCCGTATCGTGAGCATCCTCTCTCGTTTCATCGGTATCATTACCCCCATGAACAGAAATCCCCCTTACACGGAGGCATCAGTGACCAAACAGGAAAAAACCGCCCTTAACATGGCCCGCTTTATCAGAAGCCAGACATTAACGCTTCTGGAGAAACTCAACGAGCTGGACGCGGATGAACAGGCAGACATCTGTGAATCGCTTCACGACCACGCTGATGAGCTTTACCGCAGCTGCCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTGCAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAACACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTTCAAGAA

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (includes SphI, XbaI, EcoRI, BamHI, XhoI)

327

452

bacterial origin

ori

ColE1 origin of replication

3881

4563

gene fragment

ROP

repressor of primer ROP sequence

3281

3457

primer

T7 forward

T7 forward sequencing primer

481

496

primer

T7 reverse

T7 reverse sequencing primer

257

275

promoter

T7 promoter

478

497

repressor binding site

lacO

lacO regulatory sequence

456

478

selectable marker

AmpR

ampicillin resistance gene

4661

5230

tag

His

N-terminal 6x His tag

387

404

trxn termination sequence

T7 term

T7 termination sequence

257

275

Gene

Gene Symbol :

PIP5K1C

Symbol Nomenclature :

PIP5K1C

SYNONYM :

LCCS3; PIP5K-GAMMA; PIP5Kgamma; PIPKIg_v4

Designation :

Full Nomenclature :

phosphatidylinositol-4-phosphate 5-kinase, type I, gamma

GENEID :

23396

GI :

31317309

GenBank Accession :

NM_012398

HGNC :

8996

MIM :

606102

Vega :

OTTHUMG00000180870

Target GenBank:

None

Pathways

NCI :

Arf6 trafficking events

NCI :

E-cadherin signaling in the nascent adherens junction

NCI :

EGF receptor (ErbB1) signaling pathway

NCI :

N-cadherin signaling events

NCI :

Nectin adhesion pathway

NCI :

RAC1 signaling pathway

NCI :

RhoA signaling pathway

NCI :

Stabilization and expansion of the E-cadherin adherens junction

Reactome :

Axon guidance

Reactome :

Developmental Biology

Reactome :

Metabolism

Reactome :

Metabolism of lipids and lipoproteins

Reactome :

Phospholipid metabolism

Reactome :

PI Metabolism

Reactome :

SEMA3A-Plexin repulsion signaling by inhibiting Integrin adhesion

Reactome :

Semaphorin interactions

Reactome :

Synthesis of PIPs at the plasma membrane

WikiPathway :

B Cell Receptor Signaling Pathway

WikiPathway :

Focal Adhesion

WikiPathway :

Regulation of Actin Cytoskeleton

Protein

Structural Annotation Wiki :

HR3657

UniProt :

O60331

HPRD :

05834

Clone

Cloning Information :

628393

Original Clone ID :

HR3657-66-658-14.9

Experimental

TargetTrack - Experimental data :

HR3657

Organism

Species Specific ID:

23396

Genome

Chromosome :

Map Location :

19p13.3

Ensembl :

ENSG00000186111

PIP5K1C (H. sapiens )In pET15_NESG (His-tagged bacterial expression vector)

Coding Sequence Details

Recommended Growth Condition:

Vector Features:

Gene

Pathways

Protein

Clone

Experimental

Organism

Genome

PIP5K1C (H. sapiens )
In pET15_NESG (His-tagged bacterial expression vector)