DNASU Plasmid | NEDD4L (H. sapiens )<br>In pCAG.OSF (Flag-tagged mammalian expression vector)

Explanation of Terms

Gene:

Gene Symbol: NEDD4L

Gene Name: neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase

Original Clone ID:

WISP12-268

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pCAG.OSF

Format:

FUSION

Source:

University of Utah

Description:

None

Comments:

"Mutation:t2169c; t298g; g299c; g300t; t874g; g875c; g876c; t1210g; g1211c; g1212c; t1363g; g1364c; g1365c, Mutation:w100a; w292a; w404a; w455a, OSF-NEDD4L(DeltaWW) Sundquist Lab Internal ID corresponding to this clone = WISP12-268, NEDD4L-DeltaWW"

Mutation / Discrepancy:

Yes / No

Publications:

None

Authors:

University of Utah
Wesley I. Sundquist
Jun Arii

HsCD00671540

Price:

AVAILABLE

No restriction
Special MTA: SPTA

Insert Sequence
Protein Sequence

Insert sequence: 2565nts

Open reading frame: 1 to 2565

ATGGAGCGACCCTATACATTTAAGGACTTTCTCCTCAGACCAAGAAGTCATAAGTCTCGA
GTTAAGGGATTTTTGCGATTGAAAATGGCCTATATGCCAAAAAATGGAGGTCAAGATGAA
GAAAACAGTGACCAGAGGGATGACATGGAGCATGGATGGGAAGTTGTTGACTCAAATGAC
TCGGCTTCTCAGCACCAAGAGGAACTTCCTCCTCCTCCTCTGCCTCCCGGGTGGGAAGAA
AAAGTGGACAATTTAGGCCGAACTTACTATGTCAACCACAACAACCGGACCACTCAGGCT
CACAGACCAAGCCTGATGGACGTGTCCTCGGAGTCGGACAATAACATCAGACAGATCAAC
CAGGAGGCAGCACACCGGCGCTTCCGCTCCCGCAGGCACATCAGCGAAGACTTGGAGCCC
GAGCCCTCGGAGGGCGGGGATGTCCCCGAGCCTTGGGAGACCATTTCAGAGGAAGTGAAT
ATCGCTGGAGACTCTCTCGGTCTGGCTCTGCCCCCACCACCGGCCTCCCCAGGATCTCGG
ACCAGCCCTCAGGAGCTGTCAGAGGAACTAAGCAGAAGGCTTCAGATCACTCCAGACTCC
AATGGGGAACAGTTCAGCTCTTTGATTCAAAGAGAACCCTCCTCAAGGTTGAGGTCATGC
AGTGTCACCGACGCAGTTGCAGAACAGGGCCATCTACCACCGCCCAGTGCCCCAGCTGGG
AGAGCGCGTTCATCAACTGTCACGGGTGGTGAGGAACCAACGCCATCAGTGGCCTATGTA
CATACCACGCCGGGTCTGCCTTCAGGCTGGGAAGAAAGAAAAGATGCTAAGGGGCGCACA
TACTATGTCAATCATAACAATCGAACCACAACTGCCACTCGACCTATCATGCAGCTTGCA
GAAGATGGTGCGTCCGGATCAGCCACAAACAGTAACAACCATCTAATCGAGCCTCAGATC
CGCCGGCCTCGTAGCCTCAGCTCGCCAACAGTAACTTTATCTGCCCCGCTGGAGGGTGCC
AAGGACTCACCCGTACGTCGGGCTGTGAAAGACACCCTTTCCAACCCACAGTCCCCACAG
CCATCACCTTACAACTCCCCCAAACCACAACACAAAGTCACACAGAGCTTCTTGCCACCC
GGCTGGGAAATGAGGATAGCGCCAAACGGCCGGCCCTTCTTCATTGATCATAACACAAAG
ACTACAACCGCCGAAGATCCACGTTTGAAATTTCCAGTACATATGCGGTCAAAGACATCT
TTAAACCCCAATGACCTTGGCCCCCTTCCTCCTGGCTGGGAAGAAAGAATTCACTTGGAT
GGCCGAACGTTTTATATTGATCATAATAGCAAAATTACTCAGGCCGAAGACCCAAGACTG
CAGAACCCAGCTATTACTGGTCCGGCTGTCCCTTACTCCAGAGAATTTAAGCAGAAATAT
GACTACTTCAGGAAGAAATTAAAGAAACCTGCTGATATCCCCAATAGGTTTGAAATGAAA
CTTCACAGAAATAACATATTTGAAGAGTCCTATCGGAGAATTATGTCCGTGAAAAGACCA
GATGTCCTAAAAGCTAGACTGTGGATTGAGTTTGAATCAGAGAAAGGTCTTGACTATGGG
GGTGTGGCCAGAGAATGGTTCTTCTTACTGTCCAAAGAGATGTTCAACCCCTACTACGGC
CTCTTTGAGTACTCTGCCACGGACAACTACACCCTTCAGATCAACCCTAATTCAGGCCTC
TGTAATGAGGATCATTTGTCCTACTTCACTTTTATTGGAAGAGTTGCTGGTCTGGCCGTA
TTTCATGGGAAGCTCTTAGATGGTTTCTTCATTAGACCATTTTACAAGATGATGTTGGGA
AAGCAGATAACCCTGAATGACATGGAATCTGTGGATAGTGAATATTACAACTCTTTGAAA
TGGATCCTGGAGAATGACCCTACTGAGCTGGACCTCATGTTCTGCATAGACGAAGAAAAC
TTTGGACAGACATATCAAGTGGATTTGAAGCCCAATGGGTCAGAAATAATGGTCACAAAT
GAAAACAAAAGGGAATATATCGACTTAGTCATCCAGTGGAGATTTGTGAACAGGGTCCAG
AAGCAGATGAACGCCTTCTTGGAGGGATTCACAGAACTACTTCCTATTGATTTGATTAAA
ATTTTTGACGAAAATGAGCTGGAGTTGCTCATGTGCGGCCTCGGTGATGTGGATGTGAAT
GACTGGAGACAGCATTCTATTTACAAGAACGGCTACTGCCCAAACCACCCCGTCATTCAG
TGGTTCTGGAAGGCTGTGCTACTCATGGACGCCGAAAAGCGTATCCGGTTACTGCAGTTT
GTCACAGGGACATCGCGAGTACCTATGAATGGATTTGCCGAACTTTATGGTTCCAATGGT
CCTCAGCTGTTTACAATAGAGCAATGGGGCAGTCCTGAGAAACTGCCCAGAGCTCACACA
TGCTTTAATCGCCTTGACTTACCTCCATATGAAACCTTTGAAGATTTACGAGAGAAACTT
CTCATGGCCGTGGAAAATGCTCAAGGATTTGAAGGGGTGGATTAA

Protein sequence predicted based on nucleotide sequence

MERPYTFKDFLLRPRSHKSRVKGFLRLKMAYMPKNGGQDEENSDQRDDMEHGWEVVDSND
SASQHQEELPPPPLPPGWEEKVDNLGRTYYVNHNNRTTQAHRPSLMDVSSESDNNIRQIN
QEAAHRRFRSRRHISEDLEPEPSEGGDVPEPWETISEEVNIAGDSLGLALPPPPASPGSR
TSPQELSEELSRRLQITPDSNGEQFSSLIQREPSSRLRSCSVTDAVAEQGHLPPPSAPAG
RARSSTVTGGEEPTPSVAYVHTTPGLPSGWEERKDAKGRTYYVNHNNRTTTATRPIMQLA
EDGASGSATNSNNHLIEPQIRRPRSLSSPTVTLSAPLEGAKDSPVRRAVKDTLSNPQSPQ
PSPYNSPKPQHKVTQSFLPPGWEMRIAPNGRPFFIDHNTKTTTAEDPRLKFPVHMRSKTS
LNPNDLGPLPPGWEERIHLDGRTFYIDHNSKITQAEDPRLQNPAITGPAVPYSREFKQKY
DYFRKKLKKPADIPNRFEMKLHRNNIFEESYRRIMSVKRPDVLKARLWIEFESEKGLDYG
GVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFTFIGRVAGLAV
FHGKLLDGFFIRPFYKMMLGKQITLNDMESVDSEYYNSLKWILENDPTELDLMFCIDEEN
FGQTYQVDLKPNGSEIMVTNENKREYIDLVIQWRFVNRVQKQMNAFLEGFTELLPIDLIK
IFDENELELLMCGLGDVDVNDWRQHSIYKNGYCPNHPVIQWFWKAVLLMDAEKRIRLLQF
VTGTSRVPMNGFAELYGSNGPQLFTIEQWGSPEKLPRAHTCFNRLDLPPYETFEDLREKL
LMAVENAQGFEGVD*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pCAG.OSF

Synonyms:

Sequencing Primer:

Forward: CAGF

Reverse: CAGR

Description:

Mammalian expression vector with a CBA promoter and N-terminal Flag tag; ampicillin resistance in bacteria; restriction enzyme cloning (Cla1 and Kpn1)

Comments:

Empty vector Sundquist Lab internal database number = WISP 06-65. Myc tag sequence of pCAG.Myc was substituted with One-STrEP-FLAG tag sequence to create this vector. Restriction enzyme cloning can be used with this vector - e.g. Cla1, Kpn1 works well

Size (bp):

4980

Parent Vector:

None

Properties:

mammalian expression, multiple cloning site, with tag/fusion/marker

Author Name:

University of Utah
Center for Membrane Proteins in Infectious Diseases
Kathy Sykes
Brenda Hogue

Publications:

PMID: 19525971

Title: Structural basis for ESCRT-III protein autoinhibition.

Vector Map:

Vector Sequence:

Sequence:

Not Available

Vector Features:

Type

Name

Description

Start

End

bacterial origin

pBR322 origin

primer

CAGF

forward sequencing primer (5' TCGGCTTCTGGCGTGTGACC 3' )

1606

1625

primer

CAGR

reverse sequencing primer (5' TGTCCCCATAATTTTTGGCAGAGG 3')

2010

2033

promoter

CBA

Chicken beta-actin (CBA) promoter

protease cleavage site

Enterokinase

enterokinase (EK) cleavage recognition sequence

selectable marker

AmpR

ampicillin resistance marker

3992

4651

tag

Flag

N-terminal Flag tag

1858

1881

Gene

Gene Symbol :

NEDD4L

Symbol Nomenclature :

NEDD4L

SYNONYM :

NEDD4-2; NEDD4.2; RSP5; hNEDD4-2

Designation :

E3 ubiquitin-protein ligase NEDD4-like|ubiquitin-protein ligase Rsp5

Full Nomenclature :

neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase

GENEID :

23327

GenBank Accession :

NP_001138436

HGNC :

7728

MIM :

606384

Vega :

OTTHUMG00000179875

Target GenBank:

NP_001138436

Pathways

NCI :

Neurotrophic factor-mediated Trk receptor signaling

NCI :

p73 transcription factor network

NCI :

TGF-beta receptor signaling

Reactome :

Adaptive Immune System

Reactome :

Antigen processing: Ubiquitination & Proteasome degradation

Reactome :

Budding and maturation of HIV virion

Reactome :

Class I MHC mediated antigen processing & presentation

Reactome :

Disease

Reactome :

Downregulation of SMAD2/3:SMAD4 transcriptional activity

Reactome :

Downregulation of TGF-beta receptor signaling

Reactome :

Gene Expression

Reactome :

Generic Transcription Pathway

Reactome :

HIV Infection

Reactome :

HIV Life Cycle

Reactome :

Immune System

Reactome :

Ion channel transport

Reactome :

Late Phase of HIV Life Cycle

Reactome :

Loss of Function of SMAD2/3 in Cancer

Reactome :

Loss of Function of SMAD4 in Cancer

Reactome :

Loss of Function of TGFBR1 in Cancer

Reactome :

Loss of Function of TGFBR2 in Cancer

Reactome :

Signal Transduction

Reactome :

Signaling by TGF-beta Receptor Complex

Reactome :

Signaling by TGF-beta Receptor Complex in Cancer

Reactome :

SMAD2/3 MH2 Domain Mutants in Cancer

Reactome :

SMAD2/3 Phosphorylation Motif Mutants in Cancer

Reactome :

SMAD4 MH2 Domain Mutants in Cancer

Reactome :

Stimuli-sensing channels

Reactome :

TGF-beta receptor signaling activates SMADs

Reactome :

TGFBR1 KD Mutants in Cancer

Reactome :

TGFBR1 LBD Mutants in Cancer

Reactome :

TGFBR2 Kinase Domain Mutants in Cancer

Reactome :

TGFBR2 MSI Frameshift Mutants in Cancer

Reactome :

Transcriptional activity of SMAD2/SMAD3:SMAD4 heterotrimer

Reactome :

Transmembrane transport of small molecules

WikiPathway :

TGF beta Signaling Pathway

Protein

HPRD :

05903

Clone

Cloning Information :

691376

Original Clone ID :

WISP12-268

Organism

Species Specific ID:

23327

Genome

Chromosome :

Map Location :

18q21

Ensembl :

ENSG00000049759

NEDD4L (H. sapiens )In pCAG.OSF (Flag-tagged mammalian expression vector)

Coding Sequence Details

Recommended Growth Condition:

Vector Features:

Gene

Pathways

Protein

Clone

Organism

Genome

NEDD4L (H. sapiens )
In pCAG.OSF (Flag-tagged mammalian expression vector)