Gene:
Gene Symbol:
UBE2D3
Gene Name:
ubiquitin-conjugating enzyme E2D 3
Original Clone ID:
WISP14-34
Keyword:
Ube2D3
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pET11HisPP
Format:
CLOSED
Source:
University of Utah
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
University of Utah
Wesley I. Sundquist
Devin Christensen
Wesley I. Sundquist
Devin Christensen
HsCD00671580
Price:
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AVAILABLE
No restriction
Special MTA: SPTA
Special MTA: SPTA
Insert sequence: 438nts
Open reading frame: 1 to 438
ATGGCGCTGAAACGGATTAATAAGGAACTTAGTGATTTGGCCCGTGACCCTCCAGCACAA
TGTTCTGCAGGTCCAGTTGGGGATGATATGTTTCATTGGCAAGCCACAATTATGGGACCT
AATGACAGCCCATATCAAGGCGGTGTATTCTTTTTGACAATTCATTTTCCTACAGACTAC
CCCTTCAAACCACCTAAGGTTGCATTTACAACAAGAATTTATCATCCAAATATTAACAGT
AATGGCAGCATTTGTCTCGATATTCTAAGATCACAGTGGTCGCCTGCTTTAACAATTTCT
AAAGTTCTTTTATCCATTTGTTCACTGCTATGTGATCCAAACCCAGATGACCCCCTAGTG
CCAGAGATTGCACGGATCTATAAAACAGACAGAGATAAGTACAACAGAATATCTCGGGAA
TGGACTCAGAAGTATGCCATGTGA
Protein sequence predicted based on nucleotide sequence
MALKRINKELSDLARDPPAQCSAGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTDY PFKPPKVAFTTRIYHPNINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLV PEIARIYKTDRDKYNRISREWTQKYA
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pET11HisPP
Synonyms:
WISP11-262
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Bacterial expression vector with T7 promoter and N-terminal precision protease-cleavable 7xHis tag; ampicillin resistance in bacteria; NheI, XhoI restriction enzyme cloning
Comments:
Empty vector Sundquist Lab internal database number = WISP11-262
Size (bp):
5728
Parent Vector:
None
Properties:
bacterial expression, multiple cloning site, with tag/fusion/marker
Author Name:
University of Utah
Wesley I. Sundquist
Devin Christensen
Wesley I. Sundquist
Devin Christensen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 bacterial origin
1164
1846
promoter
T7
T7 promoter
5230
5249
protease cleavage site
PP
Precision protease cleavage site
5348
5371
repressor binding site
lac operator
lac operator
5249
5271
repressor protein gene
LacI
LacI
3761
4840
selectable marker
AmpR
ampicillin resistance
407
1066
tag
His
N-terminal 7xHis tag
5321
5341
trxn termination sequence
T7 term
T7 terminator
5470
5516
