Gene:
Gene Symbol:
VPS4B
Gene Name:
vacuolar protein sorting 4 homolog B (S. cerevisiae)
Original Clone ID:
None
Keyword:
"Mutation:c6a;c12t;g18c;c426t;a429g;a432g;a435c, Vacuolar protein sorting-associated protein 4 (Vps4), C terminally Myc-His tagged resistant to siRNA siVPS4B425; Internal ID WISP08-64"
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pcDNA3.1(-)/myc-His B
Format:
FUSION
Source:
University of Utah
Description:
None
Comments:
None
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
University of Utah
Wesley I. Sundquist
Christopher P. Hill
Collin Kieffer
Wesley I. Sundquist
Christopher P. Hill
Collin Kieffer
HsCD00671620
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AVAILABLE
No restriction
Special MTA: SPTA
Special MTA: SPTA
Insert sequence: 1332nts
Open reading frame: 2 to 1333
TATGTCATCCACTTCGCCCAACCTCCAGAAAGCGATAGATCTGGCTAGCAAAGCAGCGCA
AGAAGACAAGGCTGGGAACTACGAAGAAGCCCTTCAGCTCTATCAGCATGCTGTGCAGTA
TTTTCTTCATGTCGTTAAATATGAAGCACAGGGTGATAAAGCCAAGCAAAGTATCAGGGC
AAAGTGTACAGAATATCTTGATAGAGCAGAAAAACTAAAGGAGTACCTGAAAAATAAAGA
GAAAAAAGCACAGAAGCCAGTGAAAGAAGGACAGCCGAGTCCAGCAGATGAGAAGGGGAA
TGACAGTGATGGGGAAGGAGAATCTGATGATCCTGAAAAAAAGAAACTACAGAATCAACT
TCAAGGTGCCATTGTTATAGAACGACCAAATGTGAAATGGAGTGACGTTGCTGGACTTGA
AGGAGCTAAGGAGGCCCTGAAAGAGGCTGTGATACTGCCTATTAAATTTCCTCATCTTTT
TACAGGCAAGAGAACACCTTGGAGGGGAATCCTATTATTTGGGCCGCCTGGAACAGGAAA
GTCCTACTTAGCCAAAGCTGTAGCAACAGAAGCCAACAACTCAACATTTTTTTCAATATC
TTCCTCTGATCTTGTTTCTAAGTGGCTAGGTGAAAGTGAAAAACTGGTTAAGAATTTATT
CCAACTTGCCAGAGAGAACAAGCCCTCCATTATCTTCATTGATGAAATTGATTCTCTCTG
TGGTTCAAGAAGTGAAAATGAAAGTGAAGCCGCACGTAGAATTAAGACGGAGTTCCTAGT
GCAAATGCAAGGGGTTGGTGTAGACAATGATGGAATTTTGGTTCTGGGAGCTACAAATAT
ACCCTGGGTTCTGGATTCTGCCATTAGGCGAAGATTTGAGAAACGAATTTATATTCCCTT
GCCGGAACCCCATGCCCGAGCAGCAATGTTTAAACTGCACCTAGGGACCACTCAGAACAG
TCTCACGGAAGCAGACTTTCGGGAACTTGGGAGGAAAACAGATGGTTATTCAGGGGCAGA
TATAAGTATCATTGTACGTGATGCCCTTATGCAGCCTGTTAGGAAAGTACAGTCAGCTAC
TCATTTTAAAAAGGTTCGCGGACCTTCCCGAGCTGATCCTAACCATCTTGTAGATGATCT
GCTAACACCTTGCTCTCCAGGTGACCCTGGTGCCATTGAAATGACATGGATGGATGTCCC
TGGAGATAAACTTTTGGAGCCAGTTGTTTCCATGTCGGATATGTTGCGGTCACTATCTAA
CACAAAACCTACAGTCAATGAACATGACTTGTTGAAATTAAAGAAGTTTACAGAAGATTT
TGGTCAAGAAGGCGG
Protein sequence predicted based on nucleotide sequence
MSSTSPNLQKAIDLASKAAQEDKAGNYEEALQLYQHAVQYFLHVVKYEAQGDKAKQSIRA KCTEYLDRAEKLKEYLKNKEKKAQKPVKEGQPSPADEKGNDSDGEGESDDPEKKKLQNQL QGAIVIERPNVKWSDVAGLEGAKEALKEAVILPIKFPHLFTGKRTPWRGILLFGPPGTGK SYLAKAVATEANNSTFFSISSSDLVSKWLGESEKLVKNLFQLARENKPSIIFIDEIDSLC GSRSENESEAARRIKTEFLVQMQGVGVDNDGILVLGATNIPWVLDSAIRRRFEKRIYIPL PEPHARAAMFKLHLGTTQNSLTEADFRELGRKTDGYSGADISIIVRDALMQPVRKVQSAT HFKKVRGPSRADPNHLVDDLLTPCSPGDPGAIEMTWMDVPGDKLLEPVVSMSDMLRSLSN TKPTVNEHDLLKLKKFTEDFGQEG
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Host Type: mammalian Marker: neomycin
Host Type: mammalian Marker: neomycin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pcDNA3.1(-)/myc-His B
Synonyms:
''
Description:
Mammalian expression vector with CMV promoter and C-terminal myc and 6xHis tags; ampicillin resistance in bacterial; neomycin resistance in mammalian cells; restriction enzyme cloning.
Comments:
None
Size (bp):
5520
Parent Vector:
None
Properties:
mammalian expression, multiple cloning site, with tag/fusion/marker
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
Multiple cloning site
895
1006
promoter
CMV
CMV promoter
209
863
promoter
T7
T7 promoter
863
822
selectable marker
NeoR
Neomycin resistance marker
2227
3021
selectable marker
AmpR
Ampicillin resistance
4526
5386
ssDNA origin
f1
f1 origin
1389
1817
tag
myc
C-terminal myc epitope tag
1007
1036
tag
His
C-terminal 6xHis tag
1052
1069
