Gene:
Gene Symbol:
CHMP2B
Gene Name:
charged multivesicular body protein 2B
Original Clone ID:
WISP14-122 pCAG CHMP2B-myc
Keyword:
"Full length CHMP2B protein with C-terminal myc tag, Sundquist lab internal ID: WISP14-122"
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pCAG.MCS2.C-Myc
Format:
FUSION
Source:
University of Utah
Description:
None
Comments:
None
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
University of Utah
Wesley I. Sundquist
Dawn Wenzel
Wesley I. Sundquist
Dawn Wenzel
HsCD00696227
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AVAILABLE
No restriction
Special MTA: SPTA
Special MTA: SPTA
Insert sequence: 639nts
Open reading frame: 51 to 689
TATTGTGCTGTCTCATCATTTTGGCAAAGAATTATCGATCCGGAGGTACCATGGCGTCCC
TCTTCAAGAAGAAAACCGTGGATGATGTAATAAAGGAGCAAAACAGAGAGTTACGAGGTA
CACAGAGGGCTATAATCAGAGATCGAGCAGCTTTAGAGAAACAAGAAAAACAGCTGGAAT
TAGAAATTAAGAAAATGGCCAAGATTGGTAATAAGGAAGCTTGCAAAGTTTTAGCCAAAC
AACTTGTGCATCTACGGAAACAGAAGACGAGAACTTTTGCTGTAAGTTCAAAAGTTACTT
CTATGTCTACACAAACAAAAGTGATGAATTCCCAAATGAAGATGGCTGGAGCAATGTCTA
CCACAGCAAAAACAATGCAGGCAGTTAACAAGAAGATGGATCCACAAAAGACATTACAAA
CAATGCAGAATTTCCAGAAGGAAAACATGAAAATGGAAATGACTGAAGAAATGATCAATG
ATACACTTGATGACATCTTTGACGGTTCTGATGACGAAGAAGAAAGCCAGGATATTGTGA
ATCAAGTTCTTGATGAAATTGGAATTGAAATTTCTGGAAAGATGGCCAAAGCTCCATCAG
CTGCTCGAAGCTTACCATCTGCCTCTACTTCAAAGGCTACAATCTCAGATGAAGAGATTG
AACGGCAACTCAAGGCTTTAGGAGTAGATCTCGAGAAGCTTGCTAGCAGATCTGAGCAGA
AGCTGATCAGCGAGGAGGAC
Protein sequence predicted based on nucleotide sequence
MASLFKKKTVDDVIKEQNRELRGTQRAIIRDRAALEKQEKQLELEIKKMAKIGNKEACKV LAKQLVHLRKQKTRTFAVSSKVTSMSTQTKVMNSQMKMAGAMSTTAKTMQAVNKKMDPQK TLQTMQNFQKENMKMEMTEEMINDTLDDIFDGSDDEEESQDIVNQVLDEIGIEISGKMAK APSAARSLPSASTSKATISDEEIERQLKALGVD
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
G48A; A51G; C54T; A55C
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
chloramphenicol, ampicillin
Growth Condition:
LB with 35ug/ml chloramphenicol and 100ug/ml ampicillin
Comments:
None
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Protein_Soluble
Recommended expression in:
Not Applicable
Vector Name:
pCAG.MCS2.C-Myc
Synonyms:
''
Sequencing Primer:
Forward:
Unknown
Reverse:
Unknown
Description:
Mammalian expression vector with a C-terminal myc tag; ampicillin resistance in bacteria; restriction enzyme cloning.
Comments:
Sundquist Lab internal database number = WISP 08-104; Restriction enzyme cloning can be used with this vector - e.g. Xho1, Kpn1 works well
Size (bp):
4813
Parent Vector:
None
Properties:
mammalian expression, multiple cloning site, with tag/fusion/marker
Author Name:
University of Utah
Wesley I. Sundquist
Wesley I. Sundquist
Publications:
PMID:
19525971
Title: Structural basis for ESCRT-III protein autoinhibition.
Vector Map:
Vector Sequence:
Sequence:
Not Available
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3045
3727
primer
forward primer
CAGF forward sequencing primer
1605
1624
primer
reverse primer
CAGR reverse sequencing primer TGTCCCCATAATTTTTGGCAGAGG
1845
1868
selectable marker
AmpR
Ampicillin resistance
3824
4484
tag
c-myc
C-terminal may tag
0
0
