Gene:
Gene Symbol:
CHMP4A
Gene Name:
charged multivesicular body protein 4A
Original Clone ID:
WISP08-79 pCAG CHMP4A-myc
Keyword:
"Full length CHMP4A protein with C-terminal myc tag, Sundquist lab internal ID: WISP08-79"
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pCAG.MCS2.C-Myc
Format:
FUSION
Source:
University of Utah
Description:
None
Comments:
None
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
University of Utah
Wesley I. Sundquist
Uta von Schwedler
Wesley I. Sundquist
Uta von Schwedler
HsCD00696229
Price:
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AVAILABLE
No restriction
Special MTA: SPTA
Special MTA: SPTA
Insert sequence: 666nts
Open reading frame: 51 to 716
AATTATCGATCCGGAGGTACCAGAAGCTGATCTCAGAGGAGGACCTGCATATGAGCGGTC
TGGGCCGGCTCTTCGGGAAGGGGAAGAAGGAGAAAGGGCCAACCCCTGAAGAAGCAATAC
AGAAACTGAAGGAGACAGAGAAGATACTGATCAAGAAACAGGAATTTTTGGAGCAGAAGA
TTCAACAGGAGCTACAAACAGCCAAGAAGTATGGGACCAAGAATAAGAGAGCTGCCCTAC
AGGCTTTGCGGAGGAAGAAAAGATTCGAACAGCAGCTGGCACAAACTGACGGGACATTAT
CCACCCTGGAGTTTCAGCGTGAGGCCATTGAGAATGCCACTACCAATGCAGAAGTCCTTC
GTACCATGGAGCTTGCTGCCCAAAGCATGAAGAAGGCCTACCAGGACATGGACATTGACA
AGGTAGATGAACTGATGACTGACATCACGGAACAACAGGAGGTGGCCCAGCAGATCTCAG
ATGCCATTTCTCGGCCTATGGGCTTTGGAGATGATGTGGATGAGGATGAACTGCTGGAGG
AGCTAGAGGAGCTGGAGCAGGAGGAATTGGCCCAGGAGTTGTTAAATGTGGGCGACAAGG
AAGAAGAACCCTCAGTCAAATTGCCTAGTGTACCTTCTACTCATCTGCCGGCAGGGCCAG
CTCCCAAAGTGGATGAAGATGAAGAAGCACTAAAGCAGTTGGCTGAGTGGGTATCCCTCG
AGAAGCTTGCTAGCAGATCTGAGCAGAAGCTGATCAGCGAGGAGGAC
Protein sequence predicted based on nucleotide sequence
MSGLGRLFGKGKKEKGPTPEEAIQKLKETEKILIKKQEFLEQKIQQELQTAKKYGTKNKR AALQALRRKKRFEQQLAQTDGTLSTLEFQREAIENATTNAEVLRTMELAAQSMKKAYQDM DIDKVDELMTDITEQQEVAQQISDAISRPMGFGDDVDEDELLEELEELEQEELAQELLNV GDKEEEPSVKLPSVPSTHLPAGPAPKVDEDEEALKQLAEWVS
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
T6C; C12G; A16C
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
chloramphenicol, ampicillin
Growth Condition:
LB with 35ug/ml chloramphenicol and 100ug/ml ampicillin
Comments:
None
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Protein_Soluble
Recommended expression in:
Not Applicable
Vector Name:
pCAG.MCS2.C-Myc
Synonyms:
''
Sequencing Primer:
Forward:
Unknown
Reverse:
Unknown
Description:
Mammalian expression vector with a C-terminal myc tag; ampicillin resistance in bacteria; restriction enzyme cloning.
Comments:
Sundquist Lab internal database number = WISP 08-104; Restriction enzyme cloning can be used with this vector - e.g. Xho1, Kpn1 works well
Size (bp):
4813
Parent Vector:
None
Properties:
mammalian expression, multiple cloning site, with tag/fusion/marker
Author Name:
University of Utah
Wesley I. Sundquist
Wesley I. Sundquist
Publications:
PMID:
19525971
Title: Structural basis for ESCRT-III protein autoinhibition.
Vector Map:
Vector Sequence:
Sequence:
Not Available
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3045
3727
primer
forward primer
CAGF forward sequencing primer
1605
1624
primer
reverse primer
CAGR reverse sequencing primer TGTCCCCATAATTTTTGGCAGAGG
1845
1868
selectable marker
AmpR
Ampicillin resistance
3824
4484
tag
c-myc
C-terminal may tag
0
0
