Gene:
Gene Symbol:
ULK3
Gene Name:
unc-51 like kinase 3
Original Clone ID:
WISP14-119 pGEX-PP ULK3 residues 277-449 V338D
Keyword:
"Tandem MIT domains containing MIM1-binding mutation in MIT1, Tandem MIT domains of ULK3 protein with N-terminal GST tag, MIT1 MIM1 binding mutation, Sundquist lab internal ID: WISP14-119"
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pGEX2T(PP)
Format:
CLOSED
Source:
University of Utah
Description:
None
Comments:
None
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
University of Utah
Wesley I. Sundquist
Dawn Wenzel
Wesley I. Sundquist
Dawn Wenzel
HsCD00696234
Price:
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AVAILABLE
No restriction
Special MTA: SPTA
Special MTA: SPTA
Insert sequence: 522nts
Open reading frame: 51 to 572
TGGTTCCGCGTGGATCTCCGCTTGAAGTCCTCTTTCAGGGACCCCATATGAGTGGGGAGA
GTCTGGGGCGAGCAACCGCCCTGGTGGTGCAGGCTGTGAAGAAAGACCAGGAGGGGGATT
CAGCAGCCGCCTTATCACTCTACTGCAAGGCTCTGGACTTCTTTGTACCTGCCCTGCACT
ATGAAGTGGATGCCCAGCGGAAGGAGGCAATTAAGGCAAAGGTGGGGCAGTACGACTCCC
GGGCTGAGGAGCTCAAGGCCATCGTCTCCTCTTCCAATCAGGCCCTGCTGAGGCAGGGGA
CCTCTGCCCGAGACCTGCTCAGAGAGATGGCCCGGGACAAGCCACGCCTCCTAGCTGCCC
TGGAAGTGGCTTCAGCTGCCATGGCCAAGGAGGAGGCCGCCGGCGGGGAGCAGGATGCCC
TGGACCTGTACCAGCACAGCCTGGGGGAGCTACTGCTGTTGCTGGCAGCGGAGCCCCCGG
GCCGGAGGCGGGAGCTGCTTCACACTGAGGTTCAGAACCTCATGGCCCGAGCTGAATACT
TGAAGGAGCAGGTCAAGATGAGGGAATCTTGAGGATCCTCGAGAATTCATCGTGACTGAC
TGACGATCTGCCTCGCGCGTTTC
Protein sequence predicted based on nucleotide sequence
SGESLGRATALVVQAVKKDQEGDSAAALSLYCKALDFFVPALHYEVDAQRKEAIKAKVGQ YDSRAEELKAIVSSSNQALLRQGTSARDLLREMARDKPRLLAALEVASAAMAKEEAAGGE QDALDLYQHSLGELLLLLAAEPPGRRRELLHTEVQNLMARAEYLKEQVKMRES*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
V338D
(aa# referes to full length protein)
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Recommended expression in:
Not Applicable
Vector Name:
pGEX2T(PP)
Synonyms:
WISP 08-16
Description:
Bacterial expression vector with tac promoter and N-terminal GST tag and PP site; ampicillin resistance in bacteria; Nde1, BamH1 useful in addition to SLIC cloning
Comments:
Empty vector Sundquist Lab internal database number = WISP 08-16. Note: Usually use pGEX-PP-GFP for SLIC cloning and negative selection (WISP10-32)
Size (bp):
4996
Parent Vector:
None
Properties:
bacterial expression, multiple cloning site, with tag/fusion/marker
Author Name:
University of Utah
Wesley I. Sundquist
Wesley I. Sundquist
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 bacterial origin
2359
3041
primer
forward primer
pGEX5p forward sequencing primer GGGCTGGCAAGCCACGTTTGGTG
869
891
primer site
reverse primer
pGEX Reverse sequencing primer CCGGGAGCTGCATGTGTCAGAGG
1046
1068
promoter
tac promoter
tac promoter
180
208
protease cleavage site
PP
PP site
939
962
repressor binding site
lac operator
lac operator
215
237
repressor protein gene
LacI
LacI
3348
4427
selectable marker
AmpR
ampicillin resistance
1602
2261
tag
GST
N-terminal GST tag
258
929
