CENPA (Homo sapiens) in P2RP3

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
None
Keyword:
Sequence Verified: Yes; 3'UTR length: 1488; Forward Primer: GAGGAGGGACTCGGCTGA; Reverse Primer: ACACCCAAGAATGATCAATAAAAAAAAAGA
Species:
Homo sapiens
Type:
3'UTR
Vector Name:
P2RP3
Format: 
None
Source:
Center for Personalized Diagnostics
Description:
Please cite: Kotagama, K., Babb, C.S., Wolter, J.M., Murphy, R.P. and Mangone, M. "A human 3'UTR clone collection to study post-transcriptional gene regulation". BMC Genomics 16, 1036 (2015).
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
PMID: 26645212
Title: A human 3'UTR clone collection to study post-transcriptional gene regulation
Authors:
Center for Personalized Diagnostics
Marco Mangone
Kasuen Kotagama
HsUT00698449
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1488nts
Open reading frame: 1 to 1488

GATCCGGGGCCTTGAGGAGGGACTCGGCTGAGCTCCTGCACCCAGTGTTTCTGTCAGTCT
TTCCTGCTCAGCCAGGGGGTAAGCTCATCCTCTTTCACAGGACTGGGGCTGGAATTTCTC
AAGTAATTTCCTTTCTCCATCCATAGTCCCTTGTCACCTCGCCTTCCCTTTGTTCTCTAG
TAAAGGTTGATCTAGTTTTGACTGCCTGGGACAATGTGGTTTGTGCCCTTTTGTGCAATG
TTAGATAAAAGATATCTTTCTATTAAATAATAACCAGATGACCCTAATTTCTTTTGGTTG
TTATTGTTGTTGTTTTATTTTTATAAGACAGGGTCTCTGTCATCAGTGTTGCCATCGTGG
CTCACTGCAACTCCTGCCTTGGCCTCCCAAAGTGTTGAGATTACAAGAATCAGATGACCT
CATTTTGATTAAGAGAAAACTTTCTAATAAACTTTCTAATAAAAGAGAACACTTTCTAAT
AAAAACTTTCTCTTTTTTGATTAAGAGAAAGTTTTCTTACTTTTGAAAATGGCTTGTTAG
TAAGTGTGCTTCCTTAATTTCAGGGATGATACCGGGGACTCTCCAGAGCCATGACTAGAT
CCAATGGATTCTGCGATGCTGTCTGGACTTTGCTGTCTCTGAACAGTATGTGTGTGTTGC
TTTAAATATTTTTCTTTTTTTTGAGAAGGAGAAGACTGCATGACTTTCCTCTGTAACAGA
GGTAATATATGAGACAATCAACACCGTTCCAAAGGCCTGAAAATAATTTTCAGATAAAGA
GACTCCAAGGTTGACTTTAGTTTGTGAGTTACTCATGTGACTATTTGAGGATTTTGAAAA
CATCAGATTTGCTGTGGTATGGGAGAAAAGGCTATGTACTTATTATTTTAGCTCTTTCTG
TAATATTTACATTTTTTACCATATGTACATTTGTACTTTTATTTTACACATAAGGGAAAA
AATAAGACCACTTTGAGCAGTTGCCTGGAAGGCTGGGCATTTCCATCATATAGACCTCTG
CCCTTCAGAGTAGCCTCACCATTAGTGGCAGCATCATGTAACTGAGTGGACTGTGCTTGT
CAACGGATGTGTAGCTTTTCAGAAACTTAATTGGGGATGAATAGAAAACCTGTAAGCTTT
GATGTTCTGGTTACTTCTAGTAAATTCCTGTCAAAATCAATTCAGAAATTCTAACTTGGA
GAATTTAACATTTTACTCTTGTAAATCATAGAAGATGTATCATAACAGTTCAGAATTTTA
AAGTACATTTTCGATGCTTTTATGGGTATTTTTGTAGTTTCTTTGTAGAGAGATAATAAA
AATCAAAATATTTAATGAAAATGGACTTACTGTTACAGCATAGAGATTATACTGTACTTT
TTGTGTGTTAAAAATAAGTTCTTTATTTGTTTGCAAACAGCCACAATTTTTTTTTCTTTT
TTTTTTTTTTGAGATGGAGTCTTTTTTTTTATTGATCATTCTTGGGTGT

Protein sequence predicted based on nucleotide sequence 

DPGP*GGTRLSSCTQCFCQSFLLSQGVSSSSFTGLGLEFLK*FPFSIHSPLSPRLPFVL*
*RLI*F*LPGTMWFVPFCAMLDKRYLSIK**PDDPNFFWLLLLLFYFYKTGSLSSVLPSW
LTATPALASQSVEITRIR*PHFD*EKTF**TF**KRTLSNKNFLFFD*EKVFLLLKMAC*
*VCFLNFRDDTGDSPEP*LDPMDSAMLSGLCCL*TVCVCCFKYFSFF*EGEDCMTFLCNR
GNI*DNQHRSKGLKIIFR*RDSKVDFSL*VTHVTI*GF*KHQICCGMGEKAMYLLF*LFL
*YLHFLPYVHLYFYFTHKGKNKTTLSSCLEGWAFPSYRPLPFRVASPLVAASCN*VDCAC
QRMCSFSET*LGMNRKPVSFDVLVTSSKFLSKSIQKF*LGEFNILLL*IIEDVS*QFRIL
KYIFDAFMGIFVVSL*RDNKNQNI**KWTYCYSIEIILYFLCVKNKFFICLQTATIFFSF
FFF*DGVFFFIDHSWV

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: kanamycin
Growth Condition: Growth in 50 ug/ml of kanamycin in LB at 37 degrees
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable

 

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