GCM1 (Homo sapiens) in P2RP3

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
None
Keyword:
Sequence Verified: Yes; 3'UTR length: 1424; Forward Primer: CTGAACCTGTGTCCTTTGAGATGA; Reverse Primer: TGCAGAGAACATTTCTAATATCTGGAATAA
Species:
Homo sapiens
Type:
3'UTR
Vector Name:
P2RP3
Format: 
None
Source:
Center for Personalized Diagnostics
Description:
Please cite: Kotagama, K., Babb, C.S., Wolter, J.M., Murphy, R.P. and Mangone, M. "A human 3'UTR clone collection to study post-transcriptional gene regulation". BMC Genomics 16, 1036 (2015).
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
PMID: 26645212
Title: A human 3'UTR clone collection to study post-transcriptional gene regulation
Authors:
Center for Personalized Diagnostics
Marco Mangone
Kasuen Kotagama
HsUT00698551
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1425nts
Open reading frame: 1 to 1425

ATGCTTCTGAACCTGTGTCCTTTGAGATGACCCAAATCTTTCACTATGTGCACCCCAGCC
CCTCAAAAATGGGGAAGGGCTGAAAGAATTTCCTTAGGAAATAATTTTTAAAACATAACC
ACAGATAAATGAGAATCATGATAAGCAGTAGACAAGGCTTTTTTCTTTTTCTTTTTTCCT
TCTTTTTTTTTTTTTTTTTTTTTTTTTGAGACAGAGTCTTGCTCTTTTGCCCAGGCAGGA
ATGCAGTGGCGCCATCTCGGCTCACTGCAACCTCTGCCTCCCGGGTTTGAGTGATTCTCC
TGCGTCAGCCTCCCGAGTAGCTGGGATTACAGGCACCTGCCACTGCGCCTGGCTAATTTT
TCTATTTTTAGTAGAGACAGGGTTTTGCCATGTTGGTCAGGCTGCTCTCAAACTCCTGAT
CTCAAGTGATCTGCCCACCTCACCATCCCAAAGTGCTGGGATTACAGGCGTGAGCCATTG
TGCCCGGCTGACAAGGCTTTTTTCTTCACATGAGTAATTTTTTTGGCTTCTTCTCCTCCA
CTCTAGTATTTCCACCCTCAGTAAGGAATGGAAACCTGTCCCGTCTGGGGTGTGAAGTGC
CCTCTGCTTTTTCCCCCACATTGTTTGGGGTTCCCAGCACCTCAGCCACATTGCATGCTG
GGTTTTCATTGTCATTGTTGCAAATGAAATGGAGTAGACATGTGAAATGCTAACCTATCT
AGGACCTGATCTATGCCTTCTTGGGAACTGAGAATGAAGAAACAGAAATAGATGGTGGAA
GAACTGCCTAAGGTGTGAAAAGCTGAAGATTCTACCACTGAAGTACTGAATATGTTGTCT
AGTTGTGAGACTAGTGTGTAAAATATATGTTTTCTAGATGATTTCTGAGACTTGTGATGA
CAAGGAACCTGACCTAACCCCATTTTTCATAAAGACAAAGAACAGCAATGTTAAATTGCC
ATATGAAGAGAAACACTAGTGATGTGCGTGGTCTCCTTGCCTCTTGTTTCCTTCCCTCTT
CCTCACTGACTTCCCCTTGCACAGGGGCTTCTGTTTTATCCCATTTATTATTTGTGGCAC
CTATATCAATGTGGGGTTTGTAATAGGTGAGAAATATTTGCTTGGAGAAGCTCTTGTGCA
ATTAGCCTGGCAATGATTGGCCGGCCATGTTTGAATGCCAGTTTCTCTTCTTTGCTGAAG
TACTTTATGATAAAAGTAAGCCTAGACATAAAATCTGGAGAGAACCATGCAATAAACAAG
TATTTTTGAAATAAATTATTCCTTCTCCTTTATTAGAATAGTAACAGATGCACATGATAC
AAAATTCAAAAGTGAAAAAAAAGAAGTCTCCTAGTTCTAGTCTCCCCTCTGCAACACTGA
CTAGTTTCTTGTCTATTATTCCAGATATTAGAAATGTTCTCTGCA

Protein sequence predicted based on nucleotide sequence 

MLLNLCPLR*PKSFTMCTPAPQKWGRAERISLGNNF*NITTDK*ES**AVDKAFFFFFFP
SFFFFFFFFETESCSFAQAGMQWRHLGSLQPLPPGFE*FSCVSLPSSWDYRHLPLRLANF
SIFSRDRVLPCWSGCSQTPDLK*SAHLTIPKCWDYRREPLCPADKAFFFT*VIFLASSPP
L*YFHPQ*GMETCPVWGVKCPLLFPPHCLGFPAPQPHCMLGFHCHCCK*NGVDM*NANLS
RT*SMPSWELRMKKQK*MVEELPKV*KAEDSTTEVLNMLSSCETSV*NICFLDDF*DL**
QGT*PNPIFHKDKEQQC*IAI*RETLVMCVVSLPLVSFPLPH*LPLAQGLLFYPIYYLWH
LYQCGVCNR*EIFAWRSSCAISLAMIGRPCLNASFSSLLKYFMIKVSLDIKSGENHAINK
YF*NKLFLLLY*NSNRCT*YKIQK*KKRSLLVLVSPLQH*LVSCLLFQILEMFSA

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: kanamycin
Growth Condition: Growth in 50 ug/ml of kanamycin in LB at 37 degrees
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable

 

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