RFC1 (Homo sapiens) in P2RP3

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
None
Keyword:
Sequence Verified: Yes; 3'UTR length: 1492; Forward Primer: AAAGGAAAAGGAAAAAGTTCGAAGAAATGA; Reverse Primer: AGAAGACCTAGGGAGACGTGACTT
Species:
Homo sapiens
Type:
3'UTR
Vector Name:
P2RP3
Format: 
None
Source:
Center for Personalized Diagnostics
Description:
Please cite: Kotagama, K., Babb, C.S., Wolter, J.M., Murphy, R.P. and Mangone, M. "A human 3'UTR clone collection to study post-transcriptional gene regulation". BMC Genomics 16, 1036 (2015).
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
PMID: 26645212
Title: A human 3'UTR clone collection to study post-transcriptional gene regulation
Authors:
Center for Personalized Diagnostics
Marco Mangone
Kasuen Kotagama
HsUT00698698
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1491nts
Open reading frame: 1 to 1491

AAAGGAAAAGGAAAAAGTTCGAAGAAATGAAACCATTTTTCACTAGCGACAGCCACTTTT
TACTCTCCCTCCTGACCAGTCCAGCTGGTCTAGAGAAAGCCTTGTTTTTTCCCAGAGCAA
CCATGTTTTAGCATAATGGGATGACCTGGTGTCCCATTATAAATAAAGGGTGGTATGGCT
AGAAGGGTATGAGCAGTAGGCTTATGTACACCTCTTATAGAGGTTGATAGGACTGCTTGG
GTCCTCCACTGTCCTCTGTCAATCTAGTTAGACGTGCTTCTGAATGACTGTAGAATTGGA
ACTAGAAACTACACCTGGGCTTTGGAGTCAGATTTTAGTTAACAATAATGAGCCTGGGAG
CAGTAGTACTAAGGCGTCTTTTGTAGGCTTAAGAATTTATCCTAATGGCCTTTATAGGAC
CAATGCTGATTTTTTTTAAAGGCAGTTCCTATTATGTGGTGAAATTTTGTAAATAAATGA
TTATACAAAACAGTCACCACCTAGAACTGGGTATTCTTTGTACATTGTCAGATATCTTTC
AAAGTATAAATTAGGAATAAAAATGTTCCACATGATATGTGTACAAATTCTTTTTAAGAT
TGTCATCTTGTACAGCAAAATATTATGTTGGTATATTACTTCCTTATTAATTTGCAAATG
ATTGGATTAAAAAAGCTCACTGTATTCCTTACTAACCTGTTGTGTGAAACATAACATTGG
CAGTCGAGGAGTCAGAACTCGGATTCCCAGCCTCACTCCCTCTGTGTGTGTTGACCACGT
CTCTGCTGTGAATAGGATTTCATGCAGTGGTCTGTTATGGGGCTTTGCAGCGTGGGGGGC
TGCAGAAGATTCTCAGACATTCTTATCCTTCTGGAGTCTCAACCCATCAGGTTCAGACCA
GTAGGAACCAGGCTGGGTCAGGCTCTTAATTTCACTATGGTGGGGGACAAAATGAACACT
GGCTTTTGTTCACTTTGCCCCTGAGAGATCTAGTCCTGTCCTGTGATGGCTTCCACAAAC
CCTGTTGGAAGTTTGGGTTATTCCTATAACTGTATGTGTTGAGTGGCCGTGAGGCTATGG
ACCTAGCCTGGAATTTAGATGTCTAGCTATTTTTTAATCTTGTTGGTAAATGCTCAGAGG
TCTGTGCTACCTCTGTGAGGTATATACTAAAATGAATGTAAAATAAGGATCGTATTCCCT
GCTTTAAGGAACTGGTAGTCTTGTTTGGGTGATGAGACAAAGTTCTTGAGACATTTAAAC
TGTATTTACCAGTGTGAAAAATGTCATTTATGGAGATTAATTCATTATGGAAATAAAACA
TTGCCTAAGCCCTTGCCTGCTGAGTCACGACCCTGTCTTTGTTAAAGTCCATCTTGAAAT
TCTTATGCTTCAGGAAAGGTTTTCTCACCAGTAGGGTGAGGCTGGCTTTCCTATTCATGT
TCTTCCATTTCTATGCCTTTTGGATATGCAAGTCACGTCTCCCTAGGTCTTCT

Protein sequence predicted based on nucleotide sequence 

KGKGKSSKK*NHFSLATATFYSPS*PVQLV*RKPCFFPEQPCFSIMG*PGVPL*IKGGMA
RRV*AVGLCTPLIEVDRTAWVLHCPLSI*LDVLLNDCRIGTRNYTWALESDFS*Q**AWE
Q*Y*GVFCRLKNLS*WPL*DQC*FFLKAVPIMW*NFVNK*LYKTVTT*NWVFFVHCQISF
KV*IRNKNVPHDMCTNSF*DCHLVQQNIMLVYYFLINLQMIGLKKLTVFLTNLLCET*HW
QSRSQNSDSQPHSLCVC*PRLCCE*DFMQWSVMGLCSVGGCRRFSDILILLESQPIRFRP
VGTRLGQALNFTMVGDKMNTGFCSLCP*EI*SCPVMASTNPVGSLGYSYNCMC*VAVRLW
T*PGI*MSSYFLILLVNAQRSVLPL*GIY*NECKIRIVFPALRNW*SCLGDETKFLRHLN
CIYQCEKCHLWRLIHYGNKTLPKPLPAESRPCLC*SPS*NSYASGKVFSPVG*GWLSYSC
SSISMPFGYASHVSLGL

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: kanamycin
Growth Condition: Growth in 50 ug/ml of kanamycin in LB at 37 degrees
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable

 

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