Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
Sequence Verified: Yes; 3'UTR length: 2205; Forward Primer: AAGAACAGCGCTACCCCTTAA; Reverse Primer: CCCAGGCCGCTGGAAGCC
Species:
Homo sapiens
Type:
3'UTR
Vector Name:
P2RP3
Format:
None
Source:
Center for Personalized Diagnostics
Description:
Please cite: Kotagama, K., Babb, C.S., Wolter, J.M., Murphy, R.P. and Mangone, M. "A human 3'UTR clone collection to study post-transcriptional gene regulation". BMC Genomics 16, 1036 (2015).
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
Authors:
Center for Personalized Diagnostics
Marco Mangone
Kasuen Kotagama
Marco Mangone
Kasuen Kotagama
HsUT00699224
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 2205nts
Open reading frame: 1 to 2205
GCCAAGGTGAAGAACAGCGCTACCCCTTAAGAGATCTCCTTGCCTGGGTGGGAGGAGCGA
AAGTGGGGGTGTCCTGGGGAGACCAGGAACCTGCCAAGCCCAGGCTGGGGCCAAGGACTC
TGCTGAGAGGCCCCTAGAGACAACACCCTTCCCAGGCCACTGGCTGCTGGACTGTTCCTC
AGGAGCGGCCTGGGTACCCAGTATGTGCAGGGAGACGGAACCCCATGTGACAGCCCACTC
CACCAGGGTTCCCAAAGAACCTGGCCCAGTCATAATCATTCATCCTGACAGTGGCAATAA
TCACGATAACCAGTACTAGCTGCCATGATCGTTAGCCTCATATTTTCTATCTAGAGCTCT
GTAGAGCACTTTAGAAACCGCTTTCATGAATTGAGCTAATTATGAATAAATTTGGAAGGC
GATCCCTTTGCAGGGAAGCTTTCTCTCAGACCCCCTTCCATTACACCTCTCACCCTGGTA
ACAGCAGGAAGACTGAGGAGAGGGGAACGGGCAGATTCGTTGTGTGGCTGTGATGTCCGT
TTAGCATTTTTCTCAGCTGACAGCTGGGTAGGTGGACAATTGTAGAGGCTGTCTCTTCCT
CCCTCCTTGTCCACCCCATAGGGTGTACCCACTGGTCTTGGAAGCACCCATCCTTAATAC
GATGATTTTTCTGTCGTGTGAAAATGAAGCCAGCAGGCTGCCCCTAGTCAGTCCTTCCTT
CCAGAGAAAAAGAGATTTGAGAAAGTGCCTGGGTAATTCACCATTAATTTCCTCCCCCAA
ACTCTCTGAGTCTTCCCTTAATATTTCTGGTGGTTCTGACCAAAGCAGGTCATGGTTTGT
TGAGCATTTGGGATCCCAGTGAAGTAGATGTTTGTAGCCTTGCATACTTAGCCCTTCCCA
GGCACAAACGGAGTGGCAGAGTGGTGCCAACCCTGTTTTCCCAGTCCACGTAGACAGATT
CACAGTGCGGAATTCTGGAAGCTGGAGACAGACGGGCTCTTTGCAGAGCCGGGACTCTGA
GAGGGACATGAGGGCCTCTGCCTCTGTGTTCATTCTCTGATGTCCTGTACCTGGGCTCAG
TGCCCGGTGGGACTCATCTCCTGGCCGCGCAGCAAAGCCAGCGGGTTCGTGCTGGTCCTT
CCTGCACCTTAGGCTGGGGGTGGGGGGCCTGCCGGCGCATTCTCCACGATTGAGCGCACA
GGCCTGAAGTCTGGACAACCCGCAGAACCGAAGCTCCGAGCAGCGGGTCGGTGGCGAGTA
GTGGGGTCGGTGGCGAGCAGTTGGTGGTGGGCCGCGGCCGCCACTACCTCGAGGACATTT
CCCTCCCGGAGCCAGCTCTCCTAGAAACCCCGCGGCGGCCGCCGCAGCCAAGTGTTTATG
GCCCGCGGTCGGGTGGGATCCTAGCCCTGTCTCCTCTCCTGGGAAGGAGTGAGGGTGGGA
CGTGACTTAGACACCTACAAATCTATTTACCAAAGAGGAGCCCGGGACTGAGGGAAAAGG
CCAAAGAGTGTGAGTGCATGCGGACTGGGGGTTCAGGGGAAGAGGACGAGGAGGAGGAAG
ATGAGGTCGATTTCCTGATTTAAAAAATCGTCCAAGCCCCGTGGTCCAGCTTAAGGTCCT
CGGTTACATGCGCCGCTCAGAGCAGGTCACTTTCTGCCTTCCACGTCCTCCTTCAAGGAA
GCCCCATGTGGGTAGCTTTCAATATCGCAGGTTCTTACTCCTCTGCCTCTATAAGCTCAA
ACCCACCAACGATCGGGCAAGTAAACCCCCTCCCTCGCCGACTTCGGAACTGGCGAGAGT
TCAGCGCAGATGGGCCTGTGGGGAGGGGGCAAGATAGATGAGGGGGAGCGGCATGGTGCG
GGGTGACCCCTTGGAGAGAGGAAAAAGGCCACAAGAGGGGCTGCCACCGCCACTAACGGA
GATGGCCCTGGTAGAGACCTTTGGGGGTCTGGAACCTCTGGACTCCCCATGCTCTAACTC
CCACACTCTGCTATCAGAAACTTAAACTTGAGGATTTTCTCTGTTTTTCACTCGCAATAA
ATTCAGAGCAAACAAAAGATGTGGAATCTTAGAGATTTATTCCACTGGTGTGTTAGGACC
ATTAAAAAGACGTGGGCGCCCCCTTCCGACAGAGGCTCACCACTCCAGGAGAACCTGCGG
GCAGGCTTTCATCGTCCGCGGAGCTACAGGCTTCCAGCGGCCTGGG
Protein sequence predicted based on nucleotide sequence
AKVKNSATP*EISLPGWEERKWGCPGETRNLPSPGWGQGLC*EAPRDNTLPRPLAAGLFL RSGLGTQYVQGDGTPCDSPLHQGSQRTWPSHNHSS*QWQ*SR*PVLAAMIVSLIFSI*SS VEHFRNRFHELS*L*INLEGDPFAGKLSLRPPSITPLTLVTAGRLRRGERADSLCGCDVR LAFFSADSWVGGQL*RLSLPPSLSTP*GVPTGLGSTHP*YDDFSVV*K*SQQAAPSQSFL PEKKRFEKVPG*FTINFLPQTL*VFP*YFWWF*PKQVMVC*AFGIPVK*MFVALHT*PFP GTNGVAEWCQPCFPSPRRQIHSAEFWKLETDGLFAEPGL*EGHEGLCLCVHSLMSCTWAQ CPVGLISWPRSKASGFVLVLPAP*AGGGGPAGAFSTIERTGLKSGQPAEPKLRAAGRWRV VGSVASSWWWAAAATTSRTFPSRSQLS*KPRGGRRSQVFMARGRVGS*PCLLSWEGVRVG RDLDTYKSIYQRGARD*GKRPKSVSACGLGVQGKRTRRRKMRSIS*FKKSSKPRGPA*GP RLHAPLRAGHFLPSTSSFKEAPCG*LSISQVLTPLPL*AQTHQRSGK*TPSLADFGTGES SAQMGLWGGGKIDEGERHGAG*PLGERKKATRGAATATNGDGPGRDLWGSGTSGLPML*L PHSAIRNLNLRIFSVFHSQ*IQSKQKMWNLRDLFHWCVRTIKKTWAPPSDRGSPLQENLR AGFHRPRSYRLPAAW
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
kanamycin
Growth Condition:
Growth in 50 ug/ml of kanamycin in LB at 37 degrees
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
P2RP3
Synonyms:
''
Description:
Features:
ccdB – Bacterial toxin (suicide gene)
M13 reverse – Primer binding site
M13 Forward – Primer binding site
T7 – Promoter
CAT – Promoter
attP2 – Recombination site
attP3 – Recombination site
CmR – Resistance Chloramphenicol (Selectable marker)
KanR – Resistance to Kanamycin (Selectable marker)
Comments:
CPD only
Size (bp):
4773
Parent Vector:
None
Properties:
Gateway, baculovirus/insect cell expression, baculovirus/insect cell transposition, with tag/fusion/marker
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
death cassette
ccdB
Death Cassette
2084
2389
primer
M13 forward
537
553
primer
M13 reverse
3038
3054
promoter
T7
3015
3033
promoter
CAT
980
1082
recombination site
attP2
591
822
recombination site
attP3
2746
2977
selectable marker
CmR
Chloramphenicol resistance
1083
1742
selectable marker
KanR
3167
3967
