Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
Sequence Verified: Yes; 3'UTR length: 2032; Forward Primer: GGCGACAAGAACCCCTGA; Reverse Primer: GCACTGGGCCCGACTGAC
Species:
Homo sapiens
Type:
3'UTR
Vector Name:
P2RP3
Format:
None
Source:
Center for Personalized Diagnostics
Description:
Please cite: Kotagama, K., Babb, C.S., Wolter, J.M., Murphy, R.P. and Mangone, M. "A human 3'UTR clone collection to study post-transcriptional gene regulation". BMC Genomics 16, 1036 (2015).
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
Authors:
Center for Personalized Diagnostics
Marco Mangone
Kasuen Kotagama
Marco Mangone
Kasuen Kotagama
HsUT00699225
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 2031nts
Open reading frame: 1 to 2031
CACCATCAAACTTGGCGACAAGAACCCCTGAAGGAAAGGCGGGGCTCCTTCCTCAAACAT
CGGACGGCGGAATGTGGCAGATGAAATGTGCTGGAGGAAAAAAATGACATTTCTGACCTT
CCCGGGACACATTCTTCTGGAGGCTTTACATCTACTGGAGTTGAATGATTGCTGTTTTCC
TCTCACTCTCCTGTTTATTCACCAGACTGGGGATGCCTATAGGTTGTCTGTGAAATCGCA
GTCCTGCCTGGGGAGGGAGCTGTTGGCCATTTCTGTGTTTCCCTTTAAACCAGATCCTGG
AGACAGTGAGATACTCAGGGCGTTGTTAACAGGGAGTGGTATTTGAAGTGTCCAGCAGTT
GCTTGAAATGCTTTGCCGAATCTGACTCCAGTAAGAATGTGGGAAAACCCCCTGTGTGTT
CTGCAAGCAGGGCTCTTGCACCAGCGGTCTCCTCAGGGTGGACCTGCTTACAGAGCAAGC
CACGCCTCTTTCCGAGGTGAAGGTGGGACCATTCCTTGGGAAAGGATTCACAGTAAGGTT
TTTTGGTTTTTGTTTTTTGTTTTCTTGTTTTTAAAAAAAGGATTTCACAGTGAGAAAGTT
TTGGTTAGTGCATACCGTGGAAGGGCGCCAGGGTCTTTGTGGATTGCATGTTGACATTGA
CCGTGAGATTCGGCTTCAAACCAATACTGCCTTTGGAATATGACAGAATCAATAGCCCAG
AGAGCTTAGTCAAAGACGATATCACGGTCTACCTTAACCAAGGCACTTTCTTAAGCAGAA
AATATTGTTGAGGTTACCTTTGCTGCTAAAGATCCAATCTTCTAACGCCACAACAGCATA
GCAAATCCTAGGATAATTCACCTCCTCATTTGACAAATCAGAGCTGTAATTCGCTTTAAC
AAATTACGCATTTCTATCACGTTCACTAACAGCTTATGATAAGTCTGTGTAGTCTTCCTT
TTCTCCAGTTCTGTTACCCAATTTAGATTAGTAAAGCGTACACAACTGGAAAGACTGCTG
TAATAACACAGCCTTGTTATTTTTAAGTCCTATTTTGATATTAATTTCTGATTAGTTAGT
AAATAACACCTGGATTCTATGGAGGACCTCGGTCTTCATCCAAGTGGCCTGAGTATTTCA
CTGGCAGGTTGTGAATTTTTCTTTTCCTCTTTGGGGATCCAAATGATGATGTGCAATTTC
ATGTTTTAACTTGGGAAACTGAAAGTGTTCCCATATAGCTTCAAAAACAAAAACAAATGT
GTTATCCGACGGATACTTTTATGGTTACTAACTAGTACTTTCCTAATTGGGAAAGTAGTG
CTTAAGTTTGCAAATTAAGTTGGGGAGGGCAATAATAAAATGAGGGCCCGTAACAGAACC
AGTGTGTGTATAACGAAAACCATGTATAAAATGGGCCTATCACCCTTGTCAGAGATATAA
ATTACCACATTTGCCTTCCCTTCATCAGCTAACACTTATCACTTATACTACCAATAACTT
GTTAAATCAGGATTTGGCTTCATACACTGAATTTTCAGTATTTTATCTCAAGTAGATATA
GACACTAACCTTGATAGTGATACGTTAGAGGGTTCCTATTCTTCCATTGTACGATAATGT
CTTTAATATGAAATGCTACATTATTTATAATTGGTAGAGTTATTGTATCTTTTTATAGTT
GTAAGTACACAGAGGTGGTATATTTAAACTTCTGTAATATACTGTATTTAGAAATGGAAA
TATATATAGTGTTAGGTTTCACTTCTTTTAAGGTTTACCCCTGTGGTGTGGTTTAAAAAT
CTATAGGCCTGGGAATTCCGATCCTAGCTGCAGATCGCATCCCACAATGCGAGAATGATA
AAATAAAATTGGATATTTGAGAAACATTGTGCTTTTTCATTTAAAGACCAAAGTCATTTG
AATTCGATGGCAGGCCTTTCTCTTTCTGAGACCTGTACGAAATGGCTGATATTAAAGCAG
GATCAATAATGTAAGCATAAAATTACATTTGCTCAGTCAGTCGGGCCCAGTGC
Protein sequence predicted based on nucleotide sequence
HHQTWRQEPLKERRGSFLKHRTAECGR*NVLEEKNDISDLPGTHSSGGFTSTGVE*LLFS SHSPVYSPDWGCL*VVCEIAVLPGEGAVGHFCVSL*TRSWRQ*DTQGVVNREWYLKCPAV A*NALPNLTPVRMWENPLCVLQAGLLHQRSPQGGPAYRASHASFRGEGGTIPWERIHSKV FWFLFFVFLFLKKGFHSEKVLVSAYRGRAPGSLWIAC*H*P*DSASNQYCLWNMTESIAQ RA*SKTISRSTLTKALS*AENIVEVTFAAKDPIF*RHNSIANPRIIHLLI*QIRAVIRFN KLRISITFTNSL**VCVVFLFSSSVTQFRLVKRTQLERLL**HSLVIFKSYFDINF*LVS K*HLDSMEDLGLHPSGLSISLAGCEFFFSSLGIQMMMCNFMF*LGKLKVFPYSFKNKNKC VIRRILLWLLTSTFLIGKVVLKFAN*VGEGNNKMRARNRTSVCITKTMYKMGLSPLSEI* ITTFAFPSSANTYHLYYQ*LVKSGFGFIH*IFSILSQVDIDTNLDSDTLEGSYSSIVR*C L*YEMLHYL*LVELLYLFIVVSTQRWYI*TSVIYCI*KWKYI*C*VSLLLRFTPVVWFKN L*AWEFRS*LQIASHNARMIK*NWIFEKHCAFSFKDQSHLNSMAGLSLSETCTKWLILKQ DQ*CKHKITFAQSVGPS
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
kanamycin
Growth Condition:
Growth in 50 ug/ml of kanamycin in LB at 37 degrees
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
P2RP3
Synonyms:
''
Description:
Features:
ccdB – Bacterial toxin (suicide gene)
M13 reverse – Primer binding site
M13 Forward – Primer binding site
T7 – Promoter
CAT – Promoter
attP2 – Recombination site
attP3 – Recombination site
CmR – Resistance Chloramphenicol (Selectable marker)
KanR – Resistance to Kanamycin (Selectable marker)
Comments:
CPD only
Size (bp):
4773
Parent Vector:
None
Properties:
Gateway, baculovirus/insect cell expression, baculovirus/insect cell transposition, with tag/fusion/marker
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
death cassette
ccdB
Death Cassette
2084
2389
primer
M13 forward
537
553
primer
M13 reverse
3038
3054
promoter
T7
3015
3033
promoter
CAT
980
1082
recombination site
attP2
591
822
recombination site
attP3
2746
2977
selectable marker
CmR
Chloramphenicol resistance
1083
1742
selectable marker
KanR
3167
3967
