ZNF547 (Homo sapiens) in P2RP3

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
None
Keyword:
Sequence Verified: Yes; 3'UTR length: 1552; Forward Primer: CTCAGACATCAGAAAGTCCACACTGGATAA; Reverse Primer: AGGTAAATTGCTGTATTTTGTGACCTGACT
Species:
Homo sapiens
Type:
3'UTR
Vector Name:
P2RP3
Format: 
None
Source:
Center for Personalized Diagnostics
Description:
Please cite: Kotagama, K., Babb, C.S., Wolter, J.M., Murphy, R.P. and Mangone, M. "A human 3'UTR clone collection to study post-transcriptional gene regulation". BMC Genomics 16, 1036 (2015).
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
PMID: 26645212
Title: A human 3'UTR clone collection to study post-transcriptional gene regulation
Authors:
Center for Personalized Diagnostics
Marco Mangone
Kasuen Kotagama
HsUT00699330
Price: 
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1551nts
Open reading frame: 1 to 1551

TCTCAGACATCAGAAAGTCCACACTGGATAAGGCCCTTATGAATGCAGTGGATATGGGAA
AGCCTTCAGTCACCAACATATTGTGGCTGGACAGCAGGCAGTACACACTGGAGAAAGACT
GAATGCCGTGAACGTGGGTAATTATGTAGGTACAGCTCTCCAGTCGCTATGTATCAGAGA
ATTCACACTGCAGAAATGTGTGTTCAGCAAACTCGGGACATTATTTTGGTTTGACTCTCA
TCTCATTAGACATTGGAGAGTTTACACTGAAGAAGAGTCTTTTCAATAAAGTAGAAAGTG
GTAAAGATTCAACATGCAAGATTGTACTTATTGGGCTTCAGAATATCCACACTAGTGAAA
GTCTTCTGAGTACAGCAAATGTGTGACATTATTTTGCTACTACTCCACACTACTTAGACA
TCATGTAGTTCACACTGGAAAAAGGCCACGTATGTGCCTTGAATGTAGCCAAAATGACGA
ACAACACCCAGAAATCTGTGATTTAGCACTGAGAACTAGTATTATATGGTTTTTAAAAAA
CAATGGTGAAGTACATGCCACATAAAATTTGCCATCTTAACTATTGTAATGTCTTGTTTA
ATACTTGAAGTACATTAACATTGTTGAGCAAAGAATATCCTGAACTCTTTATCTTGTAAA
ATGAAACTCTATAACCACCATTAAAAAAACAACTCATTCCCACATTCTTCAGTCCCTGGC
GACCACCATATTTTTAAGTCATTATGACTCTGACTATTCTTGGTACTTCTCAGAAGAAGA
ATCTTAGAGTGTTTTTCATTATTTTTTTTCACTTAGGATAATATCCTCAAAGTTCATTCA
TTTTTTAGCATGTGTCAGAAATTTTAAGGCTGAGCAATATTTCATTGTTTGCATTTACCA
CATTTCCTTTATTTCTGACATCTATTCATGGACACTTGGGTTACTTCTACCTCTGGCTAT
TGTGAATATTGCTACTACAAGCATAAGGGCACAAATATCTCTTTGAGACCCTGCTTTCAA
TTCTTATTGGGTACCCCAAAAAGTGGAACTGCTGGATCACATGGTACTTCTAACTTTAAT
TCTTTGATGAACTGAGAAACTTTTCCATAAAACTTGCACCGTTTTACATTCCTAACATTC
CACAAAAGTTGTGATTTGTCCTCATTCTAATCCATAGAAGGCAACATTTCTAACAGGTTT
CTAGGTGATTTTGTTGTTGCTGATGTAGGGACTACATTTTGAGAACTGTCCTATCCCATT
TTTATAACAATGCAGAGCTTCTCTGTAAATACCCCTTGTTTCTATGTTCTATCCTGTGAG
CCTTTTCATTTTGTTAATTTTTCCCAGTGTTTACAACCAGGAAATAGCTTGTCATCTTTG
ATACAAAACAGAAATCTTTGCAAAATACCAATTGCATTCTGTAGCCCTTGGATCATTTTC
TCCCCCAATCTATGTAGATATTTAGTGGTGTATAATGTTGTATGAGAATACATCATGGTA
AGAATACATTTTAAAGGACTTTAAGTCAGGTCACAAAATACAGCAATTTACCT

Protein sequence predicted based on nucleotide sequence

SQTSESPHWIRPL*MQWIWESLQSPTYCGWTAGSTHWRKTECRERG*LCRYSSPVAMYQR
IHTAEMCVQQTRDIILV*LSSH*TLESLH*RRVFSIK*KVVKIQHARLYLLGFRISTLVK
VF*VQQMCDIILLLLHTT*TSCSSHWKKATYVP*M*PK*RTTPRNL*FSTEN*YYMVFKK
QW*STCHIKFAILTIVMSCLILEVH*HC*AKNILNSLSCKMKLYNHH*KNNSFPHSSVPG
DHHIFKSL*L*LFLVLLRRRILECFSLFFFT*DNILKVHSFFSMCQKF*G*AIFHCLHLP
HFLYF*HLFMDTWVTSTSGYCEYCYYKHKGTNISLRPCFQFLLGTPKSGTAGSHGTSNFN
SLMN*ETFP*NLHRFTFLTFHKSCDLSSF*SIEGNISNRFLGDFVVADVGTTF*ELSYPI
FITMQSFSVNTPCFYVLSCEPFHFVNFSQCLQPGNSLSSLIQNRNLCKIPIAFCSPWIIF
SPNLCRYLVVYNVV*EYIMVRIHFKGL*VRSQNTAIY

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: kanamycin
Growth Condition: Growth in 50 ug/ml of kanamycin in LB at 37 degrees
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable