CDK2 (Homo sapiens) in P2RP3

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
None
Keyword:
Sequence Verified: Yes; 3'UTR length: 1339; Forward Primer: GTACCCCATCTTCGACTCTGA; Reverse Primer: TCACCGTCAGCACTAGCCAAAAT
Species:
Homo sapiens
Type:
3'UTR
Vector Name:
P2RP3
Format: 
None
Source:
Center for Personalized Diagnostics
Description:
Please cite: Kotagama, K., Babb, C.S., Wolter, J.M., Murphy, R.P. and Mangone, M. "A human 3'UTR clone collection to study post-transcriptional gene regulation". BMC Genomics 16, 1036 (2015).
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
PMID: 26645212
Title: A human 3'UTR clone collection to study post-transcriptional gene regulation
Authors:
Center for Personalized Diagnostics
Marco Mangone
Kasuen Kotagama
HsUT00699407
Price: 
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1338nts
Open reading frame: 1 to 1338

GACCAAGCCAGTACCCCATCTTCGACTCTGATAGCCTTCTTGAAGCCCCCAGCCCTAATC
TCACCCTCTCCTCCAGTGTGGGCTTGACCAGGCTTGGCCTTGGGCTATTTGGACTCAGGT
GGGCCCTCTGAACTTGCCTTAAACACTCACCTTCTAGTCTTGGCCAGCCAACTCTGGGAA
TACAGGGGTGAAAGGGGGGAACCAGTGAAAATGAAAGGAAGTTTCAGTATTAGATGCACT
TAAGTTAGCCTCCACCACCCTTTCCCCCTTCTCTTAGTTATTGCTGAAGAGGGTTGGTAT
AAAAATAATTTTAAAAAAGCCTTCCTACACGTTAGATTTGCCGTACCAATCTCTGAATGC
CCCATAATTATTATTTCCAGTGTTTGGGATGACCAGGATCCCAAGCCTCCTGCTGCCACA
ATGTTTATAAAGGCCAAATGATAGCGGGGGCTAAGTTGGTGCTTTTGAGAACCAAGTAAA
ACAAAACCACTGGGAGGAGTCTATTTTAAAGAATTCGGTTGAAAAAATAGATCCAATCAG
TTTATACCCTAGTTAGTGTTTTGCCTCACCTAATAGGCTGGGAGACTGAAGACTCAGCCC
GGGTGGGGCTGCAGAAAAATGATTGGCCCCAGTCCCCTTGTTTGTCCCTTCTACAGGCAT
GAGGAATCTGGGAGGCCCTGAGACAGGGATTGTGCTTCATTCCAATCTATTGCTTCACCA
TGGCCTTATGAGGCAGGTGAGAGATGTTTGAATTTTTCTCTTCCTTTTAGTATTCTTAGT
TGTTCAGTTGCCAAGGATCCCTGATCCCATTTTCCTCTGACGTCCACCTCCTACCCCATA
GGAGTTAGAAGTTAGGGTTTAGGCATCATTTTGAGAATGCTGACACTTTTTCAGGGCTGT
GATTGAGTGAGGGCATGGGTAAAAATATTTCTTTAAAAGAAGGATGAACAATTATATTTA
TATTTCAGGTTATATCCAATAGTAGAGTTGGCTTTTTTTTTTTTTTTTTGGTCATAGTGG
GTGGATTTGTTGCCATGTGCACCTTGGGGTTTTGTAATGACAGTGCTAAAAAAAAAAAGC
ATTTTTTTTTTATGATTTGTCTCTGTCACCCTTGTCCTTGAGTGCTCTTGCTATTAACGT
TATTTGTAATTTAGTTTGTAGCTCATTAAAAAAATGTGCCTAGTTTTATAGTTCATCTCT
TTCCTCCTCTTATTTACTGAAAGAATGGTGGGAGACAGAATATGAATGTGTGTATGTGAT
CATTGATTTCAGACCCCTTTTCCTAATCTCTCTGTGCATGGCTGCCCACCCCACCCCATT
TTGGCTAGTGCTGACGGTGA

Protein sequence predicted based on nucleotide sequence 

DQASTPSSTLIAFLKPPALISPSPPVWA*PGLALGYLDSGGPSELALNTHLLVLASQLWE
YRGERGEPVKMKGSFSIRCT*VSLHHPFPLLLVIAEEGWYKNNFKKAFLHVRFAVPISEC
PIIIISSVWDDQDPKPPAATMFIKAK**RGLSWCF*EPSKTKPLGGVYFKEFG*KNRSNQ
FIP*LVFCLT**AGRLKTQPGWGCRKMIGPSPLVCPFYRHEESGRP*DRDCASFQSIASP
WPYEAGERCLNFSLPFSILSCSVAKDP*SHFPLTSTSYPIGVRS*GLGIILRMLTLFQGC
D*VRAWVKIFL*KKDEQLYLYFRLYPIVELAFFFFFWS*WVDLLPCAPWGFVMTVLKKKS
IFFL*FVSVTLVLECSCY*RYL*FSL*LIKKMCLVL*FISFLLLFTERMVGDRI*MCVCD
H*FQTPFPNLSVHGCPPHPILASADG

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: kanamycin
Growth Condition: Growth in 50 ug/ml of kanamycin in LB at 37 degrees
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable

 

The Biodesign Institute at Arizona State University

DNASU Plasmid Repository

The Biodesign Institute/Arizona State University

PO Box 878008, Tempe, AZ 85287-6401 | Map

General Customer Service - Phone: (480) 965-5697 • Email: DNASUHelp@asu.edu

Payment Questions - Phone: (480) 965-4544 • Email: DNASUPay@asu.edu

Page Contact: DNASU help | Biodesign Institute

Copyright & trademark | Accessibility | Privacy | Emergency | Contact DNASU