Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
HsCD00300851
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pANT7_cGST
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00731995
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 4278nts
Open reading frame: 1 to 4275
ATGGAGAATTACTTCCAAGCAGAAGCTTACAACCTGGACAAGGTGTTAGATGAATTTGAA
CAAAACGAAGATGAAACAGTTTCTTCTACTTTATTGGATACAAAGTGGAATAAGATTCTA
GATCCCCCTTCTCACCGGCTGTCATTTAACCCTACTTTGGCCAGTGTGAATGAATCTGCA
GTTTCTAATGAGTCACAACCACAACTGAAAGTCTTCTCCCTGGCTCATTCAGCTCCCCTG
ACCACAGAGGAAGAGGATCACTGTGCTAATGGACAGGACTGTAATCTAAATCCAGAGATT
GCCACAATGTGGATTGATGAAAATGCTGTTGCAGAAGACCAGTTAATTAAGAGAAACTAT
AGTTGGGATGATCAATGCAGTGCTGTTGAAGTGGGAGAGAAGAAATGTGGAAACCTGGCT
TGTCTGCCAGATGAGAAGAATGTTCTTGTTGTAGCCGTCATGCATAACTGTGATAAAAGG
ACATTACAAAACGATTTACAGGATTGTAATAATTATAATAGTCAATCCCTTATGGATGCT
TTTAGCTGTTCACTGGATAATGAAAACAGACAAACTGATCAATTTAGTTTTAGTATAAAT
GAGTCCACTGAAAAAGATATGAATTCAGAGAAACAAATGGATCCATTGAATAGACCGAAA
ACAGAGGGGAGATCTGTTAACCATCTGTGTCCTACTTCATCTGATAGTCTAGCCAGTGTC
TGTTCCCCTTCACAATTAAAGGATGACGGAAGTATAGGTAGAGACCCCTCCATGTCTGCG
ATTACAAGTTTAACGGTTGATTCAGTAATCTCATCCCAGGGAACAGATGGATGTCCTGCT
GTTAAAAAGCAAGAGAACTATATACCAGATGAGGACCTCACTGGCAAAATCAGCTCTCCT
AGGACAGATCTAGGGAGTCCAAATTCCTTTTCCCACATGAGTGAGGGGATTTTGATGAAA
AAAGAGCCAGCAGAGGAGAGCACCACTGAAGAATCCCTCCGGTCTGGTTTACCTTTGCTT
CTCAAACCAGACATGCCTAATGGGTCTGGAAGGAATAATGACTGTGAACGGTGTTCAGAT
TGCCTTGTGCCTAATGAAGTTAGGGCTGATGAAAATGAAGGTTATGAACATGAAGAAACT
CTTGGCACTACAGAATTCCTTAATATGACAGAGCATTTCTCTGAATCTCAGGACATGACT
AATTGGAAGTTGACTAAACTAAATGAGATGAATGATAGCCAAGTAAACGAAGAAAAGGAA
AAGTTTCTACAGATTAGTCAGCCTGAGGACACTAATGGTGATAGTGGAGGACAGTGTGTT
GGATTGGCAGATGCAGGTCTAGATTTAAAAGGAACTTGCATTAGTGAAAGTGAAGAATGT
GATTTCTCCACTGTTATAGACACACCAGCAGCAAATTATCTATCTAATGGTTGTGATTCC
TATGGAATGCAAGACCCAGGTGTTTCTTTTGTTCCAAAGACTTTACCCTCCAAAGAAGAT
TCAGTAACAGAAGAAAAAGAAATAGAGGAAAGCAAGTCAGAATGCTACTCAAATATTTAT
GAACAGAGAGGAAATGAGGCCACAGAAGGGAGTGGACTACTTTTAAACAGCACTGGTGAC
CTAATGAAGAAAAATTATTTACATAATTTCTGTAGTCAAGTTCCATCAGTGCTTGGGCAA
TCTTCCCCCAAGGTAGTAGCAAGCCTGCCATCTATCAGTGTTCCTTTTGGTGGTGCAAGA
CCCAAGCAACCTTCTAATCTTAAACTTCAAATTCCAAAGCCATTATCAGACCATTTACAA
AATGACTTTCCTGCAAACAGTGGAAATAATACTAAAAATAAAAATGATATTCTTGGGAAA
GCAAAATTAGGGGAAAACTCAGCAACCAATGTATGCAGTCCATCTTTGGGAAACATCTCT
AATGTCGATACAAATGGGGAACATTTAGAAAGTTATGAGGCTGAGATCTCCACTAGACCA
TGCCTTGCATTAGCTCCAGATAGCCCAGATAATGATCTCAGAGCTGGTCAGTTTGGAATT
TCTGCCAGAAAGCCATTCACCACTCTGGGTGAGGTGGCTCCAGTATGGGTACCGGATTCT
CAGGCTCCAAATTGCATGAAATGTGAAGCCAGGTTTACATTCACCAAAAGGAGGCATCAC
TGCAGAGCATGTGGGAAGGTTTTCTGTGCTTCCTGCTGTAGCCTGAAATGTAAACTGTTA
TACATGGACAGAAAGGAAGCTAGAGTGTGTGTAATCTGCCATTCAGTGCTAATGAATGCT
CAAGCCTGGGAGAACATGATGAGTGCCTCAAGCCAGAGCCCTAACCCTAACAATCCTGCT
GAATACTGTTCTACTATCCCTCCCTTGCAGCAAGCTCAGGCCTCAGGAGCTCTGAGCTCT
CCACCTCCCACTGTGATGGTACCTGTGGGAGTTTTAAAGCACCCTGGAGCAGAAGTGGCT
CAGCCCAGAGAGCAGAGGCGAGTTTGGTTTGCTGATGGGATCTTGCCCAATGGAGAAGTT
GCTGATGCAGCCAAATTAACAATGAATGGAACTTCCTCTGCAGGAACCCTGGCTGTGTCA
CACGACCCAGTCAAGCCAGTAACTACCAGTCCTCTACCAGCAGAGACGGATATTTGTCTA
TTCTCTGGGAGTATAACTCAGGTTGGAAGTCCTGTTGGAAGTGCAATGAATCTTATTCCT
GAAGATGGCCTTCCTCCCATTCTCATCTCCACTGGTGTAAAAGGAGACTATGCTGTGGAA
GAGAAACCATCACAGATTTCAGTAATGCAGCAGTTGGAGGATGGTGGCCCTGACCCACTT
GTATTTGTTTTAAATGCAAATTTGTTGTCAATGGTTAAAATTGTAAATTATGTGAACAGG
AAGTGCTGGTGTTTCACAACCAAGGGAATGCATGCAGTGGGTCAGTCTGAGATAGTCATT
CTTCTACAGTGTTTACCGGATGAAAAGTGTTTGCCAAAGGATATCTTTAATCACTTTGTG
CAGCTTTATCGGGATGCTCTGGCAGGGAATGTGGTGAGCAACTTGGGACATTCCTTCTTC
AGTCAAAGTTTCCTTGGCAGTAAAGAACATGGTGGATTCTTATATGTGACATCTACCTAC
CAGTCACTGCAAGACCTAGTACTCCCAACCCCACCTTACTTGTTTGGGATTCTTATCCAG
AAATGGGAAACTCCTTGGGCTAAAGTATTTCCTATCCGTCTGATGTTGAGACTTGGAGCT
GAATATCGACTTTATCCATGCCCACTATTCAGTGTCAGATTTCGGAAGCCATTGTTTGGA
GAGACGGGGCATACCATCATGAATCTTCTTGCAGACTTCAGAAATTACCAGTATACCTTG
CCAGTAGTTCAAGGTTTGGTGGTTGATATGGAAGTTCGGAAAACTAGCATCAAAATTCCC
AGCAACAGATACAATGAGATGATGAAAGCCATGAACAAGTCCAATGAGCATGTCCTGGCA
GGAGGTGCCTGCTTCAATGAAAAGGCAGACTCTCATCTTGTGTGTGTACAGAATGATGAT
GGAAACTATCAGACCCAGGCTATCAGTATTCACAATCAGCCCAGAAAAGTGACTGGTGCC
AGTTTCTTTGTGTTCAGTGGCGCTCTGAAATCCTCTTCTGGATACCTTGCCAAGTCCAGT
ATTGTGGAAGATGGTGTTATGGTCCAGATTACTGCAGAGAACATGGATTCCTTGAGGCAG
GCACTGCGAGAGATGAAGGACTTCACCATCACCTGTGGGAAGGCGGACGCGGAGGAACCC
CAGGAGCACATCCACATCCAGTGGGTGGATGATGACAAGAACGTTAGCAAGGGTGTCGTA
AGTCCTATAGATGGGAAGTCCATGGAGACTATAACAAATGTGAAGATATTCCATGGATCA
GAATATAAAGCAAATGGAAAAGTAATCAGATGGACAGAGGTGTTTTTCCTAGAAAACGAT
GACCAGCACAATTGCCTCAGTGATCCTGCAGATCACAGTAGATTGACTGAGCATGTTGCC
AAAGCTTTTTGCCTTGCTCTCTGTCCTCACCTGAAACTTCTGAAGGAAGATGGAATGACC
AAACTGGGACTACGTGTGACACTTGACTCAGATCAGGTTGGCTATCAAGCAGGGAGCAAT
GGCCAGCCCCTTCCCTCGCAGTACATGAATGATCTGGACAGCGCCTTGGTGCCGGTGATC
CATGGAGGGGCCTGCCAGCTTAGTGAGGGCCCCGTTGTCATGGAACTCATCTTTTATATT
CTGGAAAACATCGTA
Protein sequence predicted based on nucleotide sequence
MENYFQAEAYNLDKVLDEFEQNEDETVSSTLLDTKWNKILDPPSHRLSFNPTLASVNESA VSNESQPQLKVFSLAHSAPLTTEEEDHCANGQDCNLNPEIATMWIDENAVAEDQLIKRNY SWDDQCSAVEVGEKKCGNLACLPDEKNVLVVAVMHNCDKRTLQNDLQDCNNYNSQSLMDA FSCSLDNENRQTDQFSFSINESTEKDMNSEKQMDPLNRPKTEGRSVNHLCPTSSDSLASV CSPSQLKDDGSIGRDPSMSAITSLTVDSVISSQGTDGCPAVKKQENYIPDEDLTGKISSP RTDLGSPNSFSHMSEGILMKKEPAEESTTEESLRSGLPLLLKPDMPNGSGRNNDCERCSD CLVPNEVRADENEGYEHEETLGTTEFLNMTEHFSESQDMTNWKLTKLNEMNDSQVNEEKE KFLQISQPEDTNGDSGGQCVGLADAGLDLKGTCISESEECDFSTVIDTPAANYLSNGCDS YGMQDPGVSFVPKTLPSKEDSVTEEKEIEESKSECYSNIYEQRGNEATEGSGLLLNSTGD LMKKNYLHNFCSQVPSVLGQSSPKVVASLPSISVPFGGARPKQPSNLKLQIPKPLSDHLQ NDFPANSGNNTKNKNDILGKAKLGENSATNVCSPSLGNISNVDTNGEHLESYEAEISTRP CLALAPDSPDNDLRAGQFGISARKPFTTLGEVAPVWVPDSQAPNCMKCEARFTFTKRRHH CRACGKVFCASCCSLKCKLLYMDRKEARVCVICHSVLMNAQAWENMMSASSQSPNPNNPA EYCSTIPPLQQAQASGALSSPPPTVMVPVGVLKHPGAEVAQPREQRRVWFADGILPNGEV ADAAKLTMNGTSSAGTLAVSHDPVKPVTTSPLPAETDICLFSGSITQVGSPVGSAMNLIP EDGLPPILISTGVKGDYAVEEKPSQISVMQQLEDGGPDPLVFVLNANLLSMVKIVNYVNR KCWCFTTKGMHAVGQSEIVILLQCLPDEKCLPKDIFNHFVQLYRDALAGNVVSNLGHSFF SQSFLGSKEHGGFLYVTSTYQSLQDLVLPTPPYLFGILIQKWETPWAKVFPIRLMLRLGA EYRLYPCPLFSVRFRKPLFGETGHTIMNLLADFRNYQYTLPVVQGLVVDMEVRKTSIKIP SNRYNEMMKAMNKSNEHVLAGGACFNEKADSHLVCVQNDDGNYQTQAISIHNQPRKVTGA SFFVFSGALKSSSGYLAKSSIVEDGVMVQITAENMDSLRQALREMKDFTITCGKADAEEP QEHIHIQWVDDDKNVSKGVVSPIDGKSMETITNVKIFHGSEYKANGKVIRWTEVFFLEND DQHNCLSDPADHSRLTEHVAKAFCLALCPHLKLLKEDGMTKLGLRVTLDSDQVGYQAGSN GQPLPSQYMNDLDSALVPVIHGGACQLSEGPVVMELIFYILENIV
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pANT7_cGST
Synonyms:
pANT7-cGST
Description:
with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments:
One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp):
5964
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Joshua LaBaer
Al Lau
Niroshan Ramachandran
Al Lau
Niroshan Ramachandran
Publications:
PMID:
15232106
Title: Self-assembling protein microarrays
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 bacterial origin of replication
3931
3249
death cassette
ccdB
ccdB death cassette present in 'empty vector' form only (replace with gene of interest)
1876
2085
primer
NAP138
NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3?
2405
2384
primer
NAP150
Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3?
388
408
promoter
T7
T7 transcriptional start sequence
5940
5964
recombination site
attR1
AttR1 site for recombination in empty vector
549
565
recombination site
attR2
AttR2 site for recombination in empty vector
2236
2251
selectable marker
CmR
chloramphenicol resistance
782
1438
selectable marker
AmpR
ampicillin resistance gene
4688
4029
tag
GST
Glutathione transferase (GST) tag
2282
2950
