Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
FUSION
Source:
None
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00740305
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 3855nts
Open reading frame: 1 to 3855
ATGGAGGATGGAAAGCCCGTTTGGGCGCCACACCCTACAGATGGATTTCAGATGGGCAAT
ATTGTGGATATTGGCCCCGACAGCTTAACAATTGAACCCTTGAATCAGAAAGGCAAGACA
TTTTTGGCTCTCATAAACCAAGTGTTTCCTGCAGAAGAGGACAGTAAAAAAGATGTGGAA
GATAACTGTTCACTAATGTATTTAAATGAAGCCACACTGCTCCATAATATCAAAGTTCGA
TATAGTAAAGACAGAATTTATACATATGTCGCCAACATTCTGATTGCAGTGAATCCATAC
TTTGACATACCTAAAATATATTCTTCAGAAGCAATAAAGTCATATCAAGGAAAATCTCTT
GGGACAAGACCACCTCATGTCTTTGCAATTGCTGATAAAGCTTTTCGAGACATGAAGGTG
CTCAAGATGAGTCAGTCTATCATTGTATCTGGAGAATCAGGAGCCGGCAAAACAGAAAAT
ACAAAATTTGTTCTAAGATACCTGACTGAATCCTATGGAACAGGTCAAGATATTGATGAC
AGAATTGTTGAAGCTAACCCACTCCTAGAAGCCTTTGGAAATGCGAAGACTGTTCGCAAC
AATAATAGCAGTCGATTTGGGAAATTTGTAGAAATACATTTTAATGAAAAGAGCTCAGTT
GTTGGAGGATTTGTTTCACATTATCTCCTAGAGAAATCTAGGATCTGTGTTCAAGGCAAA
GAGGAAAGAAATTATCATATCTTTTATAGGTTGTGTGCTGGTGCTTCTGAAGATATTAGA
GAAAAACTTCATTTGAGTTCACCAGATAATTTTCGGTATTTAAACCGAGGCTGCACTAGA
TACTTTGCTAACAAAGAAACTGACAAACAGATTTTACAGAACCGCAAAAGTCCTGAGTAC
CTTAAGGCAGGTTCTATGAAAGATCCTCTGCTAGATGACCATGGTGATTTTATTAGAATG
TGCACGGCTATGAAAAAAATTGGTTTGGATGATGAAGAAAAGCTTGATCTCTTCCGGGTA
GTAGCTGGCGTCCTGCACCTTGGAAATATTGATTTTGAGGAAGCTGGCAGCACTTCAGGT
GGTTGTAATCTGAAGAATAAATCTGCTCAGTCTTTGGAATATTGTGCTGAATTACTGGGT
TTGGACCAAGATGATCTTCGAGTAAGTTTGACCACAAGAGTCATGCTAACAACAGCAGGG
GGCACCAAAGGAACAGTTATAAAGGTACCTCTGAAAGTGGAGCAAGCAAACAATGCTCGT
GATGCCCTGGCAAAGACAGTGTATAGCCATCTTTTTGATCATGTGGTAAACAGAGTAAAT
CAGTGTTTTCCTTTTGAAACATCATCCTATTTTATTGGAGTCCTAGATATTGCTGGTTTT
GAGTACTTTGAGCATAACAGTTTTGAACAATTTTGCATCAACTATTGCAATGAAAAACTT
CAACAATTTTTTAATGAAAGGATTCTGAAGGAGGAACAAGAACTCTATCAAAAAGAAGGT
TTAGGTGTTAATGAAGTGCATTATGTGGATAATCAGGACTGTATAGATTTAATTGAAGCC
AAATTAGTGGGAATACTGGATATTTTGGATGAAGAAAATCGCCTTCCCCAGCCAAGTGAT
CAACACTTTACATCTGCAGTTCACCAAAAGCACAAGGATCATTTTCGACTCACTATTCCC
AGAAAATCTAAGCTGGCAGTTCATAGGAATATCAGAGACGACGAAGGCTTCATTATCAGG
CATTTTGCGGGGGCAGTGTGCTATGAAACAACCCAGTTTGTGGAGAAAAATAATGATGCT
TTACATATGTCTCTTGAATCCTTAATATGTGAATCCAGAGATAAGTTTATACGGGAATTA
TTTGAATCATCCACAAATAACAACAAAGATACTAAACAAAAAGCAGGAAAACTTAGCTTC
ATCAGCGTGGGAAACAAGTTTAAGACACAGTTAAATTTGCTTCTGGATAAACTTCGAAGT
ACTGGAGCAAGCTTTATTCGTTGCATCAAACCTAACTTAAAGATGACAAGCCACCACTTT
GAAGGTGCTCAAATTCTGTCTCAGCTTCAGTGTTCAGGGATGGTGTCTGTTTTGGACTTG
ATGCAGGGTGGTTACCCATCACGAGCTTCATTTCATGAACTCTACAACATGTACAAAAAG
TATATGCCAGATAAACTTGCAAGATTGGATCCAAGACTATTTTGTAAGGCTTTGTTTAAA
GCTTTGGGCTTAAATGAAAATGACTACAAGTTTGGGTTAACCAAAGTATTTTTTAGACCT
GGCAAGTTTGCAGAATTTGATCAGATCATGAAGTCTGACCCTGACCACTTAGCAGAGTTG
GTTAAAAGAGTCAATCACTGGCTCACATGCAGTCGCTGGAAGAAAGTTCAGTGGTGCTCA
CTCTCAGTCATCAAATTGAAAAACAAAATAAAATATCGAGCTGAAGCCTGCATTAAAATG
CAAAAAACTATTCGAATGTGGCTTTGCAAGAGGAGACACAAACCTCGCATTGATGGTCTG
GTTAAGGTGGGCACACTGAAAAAACGACTTGATAAATTTAATGAGGTAGTCAGTGTGTTG
AAAGATGGAAAACCCGAGATGAATAAACAGATCAAGAATCTGGAAATTTCTATTGATACT
TTGATGGCCAAAATTAAGTCCACTATGATGACGCAGGAACAAATCCAGAAAGAATATGAT
GCACTGGTTAAAAGCTCAGAGGAACTCCTCAGTGCATTACAGAAAAAAAAACAGCAGGAA
GAGGAAGCAGAAAGGCTGAGGCGTATTCAAGAAGAAATGGAAAAGGAAAGAAAAAGACGT
GAAGAAGACGAAAAACGTCGAAGAAAGGAAGAGGAGGAAAGGCGGATGAAACTTGAGATG
GAAGCAAAGAGAAAACAAGAAGAAGAAGAGAGAAAGAAAAGGGAAGATGATGAAAAACGC
ATTCAAGCTGAAGTGGAGGCACAGCTGGCCCGACAGAAGGAGGAGGAATCCCAACAGCAA
GCAGTTCTGGAGCAGGAGCGCAGGGACCGGGAGCTGGCCCTGAGGATTGCCCAGAGTGAA
GCCGAGCTCATCAGTGATGAGGCCCAGGCCGACCTGGCGCTGCGGAGAAATGATGGAACA
AGACCCAAAATGACACCGGAACAAATGGCCAAAGAAATGTCAGAATTTTTGAGTAGAGGT
CCTGCTGTACTAGCCACCAAAGCAGCTGCTGGTACTAAGAAATATGATCTTAGTAAATGG
AAATATGCAGAACTACGTGATACCATCAATACTTCTTGTGATATTGAGCTCCTGGCAGCT
TGCAGAGAAGAATTTCATAGGAGACTAAAAGTGTATCATGCTTGGAAATCTAAGAACAAG
AAGAGAAATACTGAAACAGAGCAACGTGCTCCAAAGTCTGTTACTGATTATGATTTTGCA
CCATTTTTGAACAATTCACCTCAGCAAAACCCAGCAGCTCAGATTCCTGCCAGGCAGCGG
GAGATTGAAATGAACCGACAGCAACGCTTCTTCCGCATCCCATTCATCCGCCCTGCCGAC
CAGTACAAAGACCCTCAGAGTAAGAAAAAAGGCTGGTGGTATGCCCATTTTGATGGACCA
TGGATTGCCCGGCAAATGGAACTCCATCCTGACAAGCCACCCATCCTACTTGTGGCTGGT
AAGGACGACATGGAGATGTGTGAGCTGAATCTTGAGGAGACTGGCCTGACTCGGAAGCGT
GGTGCTGAGATCTTGCCAAGACAGTTTGAAGAAATCTGGGAACGCTGTGGAGGCATCCAG
TACCTTCAGAATGCGATTGAGAGCAGACAGGCTCGGCCCACCTATGCAACAGCCATGCTG
CAGAGTCTGTTAAAG
Protein sequence predicted based on nucleotide sequence
MEDGKPVWAPHPTDGFQMGNIVDIGPDSLTIEPLNQKGKTFLALINQVFPAEEDSKKDVE DNCSLMYLNEATLLHNIKVRYSKDRIYTYVANILIAVNPYFDIPKIYSSEAIKSYQGKSL GTRPPHVFAIADKAFRDMKVLKMSQSIIVSGESGAGKTENTKFVLRYLTESYGTGQDIDD RIVEANPLLEAFGNAKTVRNNNSSRFGKFVEIHFNEKSSVVGGFVSHYLLEKSRICVQGK EERNYHIFYRLCAGASEDIREKLHLSSPDNFRYLNRGCTRYFANKETDKQILQNRKSPEY LKAGSMKDPLLDDHGDFIRMCTAMKKIGLDDEEKLDLFRVVAGVLHLGNIDFEEAGSTSG GCNLKNKSAQSLEYCAELLGLDQDDLRVSLTTRVMLTTAGGTKGTVIKVPLKVEQANNAR DALAKTVYSHLFDHVVNRVNQCFPFETSSYFIGVLDIAGFEYFEHNSFEQFCINYCNEKL QQFFNERILKEEQELYQKEGLGVNEVHYVDNQDCIDLIEAKLVGILDILDEENRLPQPSD QHFTSAVHQKHKDHFRLTIPRKSKLAVHRNIRDDEGFIIRHFAGAVCYETTQFVEKNNDA LHMSLESLICESRDKFIRELFESSTNNNKDTKQKAGKLSFISVGNKFKTQLNLLLDKLRS TGASFIRCIKPNLKMTSHHFEGAQILSQLQCSGMVSVLDLMQGGYPSRASFHELYNMYKK YMPDKLARLDPRLFCKALFKALGLNENDYKFGLTKVFFRPGKFAEFDQIMKSDPDHLAEL VKRVNHWLTCSRWKKVQWCSLSVIKLKNKIKYRAEACIKMQKTIRMWLCKRRHKPRIDGL VKVGTLKKRLDKFNEVVSVLKDGKPEMNKQIKNLEISIDTLMAKIKSTMMTQEQIQKEYD ALVKSSEELLSALQKKKQQEEEAERLRRIQEEMEKERKRREEDEKRRRKEEEERRMKLEM EAKRKQEEEERKKREDDEKRIQAEVEAQLARQKEEESQQQAVLEQERRDRELALRIAQSE AELISDEAQADLALRRNDGTRPKMTPEQMAKEMSEFLSRGPAVLATKAAAGTKKYDLSKW KYAELRDTINTSCDIELLAACREEFHRRLKVYHAWKSKNKKRNTETEQRAPKSVTDYDFA PFLNNSPQQNPAAQIPARQREIEMNRQQRFFRIPFIRPADQYKDPQSKKKGWWYAHFDGP WIARQMELHPDKPPILLVAGKDDMEMCELNLEETGLTRKRGAEILPRQFEEIWERCGGIQ YLQNAIESRQARPTYATAMLQSLLK
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
