Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
CLOSED (STOP codon in vector)
Source:
None
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00740546
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 3459nts
Open reading frame: 1 to 3459
ATGGGGGACACAGCGCCCCCGCAGGCCCCCGCAGGAGGGCTAGGGGGGGCCTCTGGGGCG
GGGCTCCTTGGAGGGGGCTCAGTCACCCCGAGAGTGCACAGTGCTATCGTGGAGCGCCTC
CGGGCTCGGATCGCTGTCTGCCGCCAACACCACCTGAGCTGTGAAGGACGATATGAACGA
GGTAGGGCCGAGAGCTCAGACCGGGAAAGAGAAAGCACCTTGCAGCTCCTGAGCCTTGTA
CAGCATGGCCAGGGGGCAAGGAAAGCTGGCAAACACACCAAGGCCACCGCCACTGCTGCC
ACCACTACAGCCCCTCCACCGCCCCCTGCTGCCCCTCCTGCGGCCTCCCAAGCAGCAGCA
ACAGCAGCCCCACCGCCCCCACCAGACTATCACCATCACCACCAGCAGCACCTGCTGAAC
AGTAGCAATAATGGTGGCAGTGGTGGGATAAACGGAGAGCAGCAGCCGCCCGCTTCAACC
CCAGGGGACCAGAGGAACTCAGCCCTGATTGCGCTCCAGGGTTCCTTGAAAAGAAAACAG
GTAGTTAACCTATCTCCTGCCAACAGCAAGCGACCCAATGGCTTTGTGGACAACTCATTT
CTTGATATCAAAAGAATTCGTGTTGGGGAGAATCTCTCTGCAGGACAAGGTGGCCTCCAA
ATAAACAATGGACAAAGTCAGATTATGTCAGGGACCTTGCCTATGAGCCAAGCACCCCTG
CGAAAGACTAACACTCTGCCATCCCATACACATTCTCCTGGCAATGGCCTGTTTAACATG
GGCTTAAAGGAGGTAAAGAAGGAGCCAGGAGAGACTCTGTCTTGCAGTAAGCACATGGAT
GGCCAAATGACCCAAGAGAATATTTTTCCTAATAGGTACGGAGACGACCCTGGAGAACAA
CTGATGGATCCTGAGCTGCAGGAACTGTTCAATGAACTGACCAACATATCTGTGCCTCCC
ATGAGTGACCTTGAACTGGAGAACATGATCAATGCCACCATAAAGCAGGATGACCCATTT
AACATTGACTTGGGTCAGCAAAGCCAGAGGAGCACACCTAGGCCCTCCTTACCCATGGAG
AAAATAGTGATCAAAAGTGAATACTCACCGGGCTTGACTCAGGGCCCCTCAGGCTCTCCT
CAGCTGAGGCCCCCATCAGCTGGCCCCGCATTCTCCATGGCCAACTCTGCCCTCTCCACT
TCGTCTCCAATCCCTTCAGTCCCTCAGAGCCAGGCTCAGCCTCAGACAGGCTCCGGAGCA
AGCCGGGCCTTGCCAAGCTGGCAGGAAGTATCCCATGCCCAGCAGCTCAAACAGATAGCT
GCTAATCGTCAGCAGCATGCCCGGATGCAGCAGCACCAGCAGCAGCACCAGCCTACCAAC
TGGTCAGCCTTGCCCTCCTCTGCTGGACCATCACCAGGTCCATTTGGGCAGGAGAAAATC
CCCAGCCCTTCTTTTGGTCAGCAGACATTCAGCCCACAGAGCTCCCCCATGCCTGGGGTA
GCTGGCGGCAGCGGCCAGTCGAAAGTAATGGCTAACTACATGTACAAGGCCGGCCCCTCA
GCCCAGGGTGGGCACCTAGATGTCCTCATGCAGCAAAAGCCTCAGGATCTCAGTCGAAGT
TTTATTAACAACCCGCACCCAGCCATGGAGCCCCGTCAGGGCAACACCAAGCCTTTGTTT
CATTTTAACTCAGATCAAGCGAACCAGCAGATGCCTTCTGTTTTGCCTTCCCAGAACAAG
CCTTCTCTCCTACACTACACCCAACAGCAACAGCAGCAACAGCAGCAGCAGCAGCAGCAG
CAGCAGCAGCAACAGCAGCAGCAGCAGCAACAGCAACAGCAACAGCAACAGCAGAGTTCA
ATTTCAGCTCAACAACAGCAACAGCAGCAGAGCTCAATTTCAGCCCAACAGCAGCAGCAG
CAGCAACAACAGCAGCAGCAGCAGCAACAACAACAGCAACAACAGCAGCAGCAGCAGCAG
CAACAACCATCTTCTCAGCCTGCCCAATCTCTACCAAGCCAGCCTTTGCTAAGGTCACCT
TTGCCACTTCAGCAAAAGCTCCTACTTCAGCAAATGCAGAATCAGCCCATTGCAGGAATG
GGATACCAAGTCTCCCAACAACAGAGACAGGATCAACACTCTGTGGTAGGCCAGAACACA
GGCCCCAGTCCAAGTCCTAACCCCTGCTCAAATCCAAACACTGGAAGTGGTTACATGAAC
TCCCAGCAATCACTGTTGAATCAGCAATTGATGGGAAAGAAGCAGACTCTACAGAGGCAG
ATCATGGAGCAGAAACAGCAACTTCTTCTCCAGCAGCAGATGCTGGCTGACGCGGAGAAA
ATTGCTCCACAAGATCAGATAAACCGACATTTGTCAAGGCCACCTCCAGATTATAAAGAC
CAAAGAAGAAATGTGGGCAATATGCAACCAACTGCTCAGTATTCTGGTGGCTCATCCACA
ATAAGCTTAAACTCTAACCAGGCTTTGGCAAACCCAGTTTCAACACACACCATTTTAACT
CCCAATTCCAGCCTCCTGTCTACTTCTCACGGGACAAGAATGCCATCATTATCTACAGCA
GTTCAGAATATGGGGATGTATGGAAATCTGCCTTGTAATCAACCTAACACATACAGTGTC
ACTTCAGGAATGAATCAATTGACCCAACAGAGAAACCCAAAGCAATTGTTAGCAAATCAA
AACAACCCTATGATGCCACGGCCACCTACCTTAGGGCCAAGTAATAATAACAATGTAGCC
ACTTTTGGAGCTGGATCTGTTGGTAATTCACAACAATTGAGACCAAATTTAACCCATAGT
ATGGCAAGCATGCCACCACAGAGAACATCAAACGTAATGATCACATCCAACACAACTGCA
CCAAACTGGGCCTCTCAAGAAGGAACAAGCAAACAGCAAGAAGCCCTGACGTCTGCAGGA
GTCCGCTTCCCCACAGGTACACCTGCAGCCTATACCCCAAATCAGTCACTGCAACAGGCA
GTAGGTAGCCAGCAATTTTCCCAGAGGGCAGTGGCTCCTCCTAACCAGTTAACACCAGCA
GTGCAAATGAGACCCATGAACCAAATGAGCCAAACACTAAATGGGCAAACCATGGGTCCC
CTCAGGGGTCTGAATCTCAGACCCAATCAGCTAAGCACACAGATTTTGCCTAATTTGAAT
CAGTCAGGAACAGGGTTGAATCAGTCGAGGACGGGCATCAACCAGCCACCATCCCTGACG
CCCAGCAATTTTCCTTCACCCAACCAAAGTTCCAGGGCTTTTCAAGGAACTGACCACAGC
AGTGACTTAGCTTTTGACTTCCTCAGCCAACAAAATGATAACATGGGCCCTGCCCTAAAC
AGTGATGCTGATTTCATTGATTCTTTATTGAAGACAGAGCCTGGTAATGATGACTGGATG
AAAGACATCAATCTTGATGAAATCTTGGGGAACAATTCC
Protein sequence predicted based on nucleotide sequence
MGDTAPPQAPAGGLGGASGAGLLGGGSVTPRVHSAIVERLRARIAVCRQHHLSCEGRYER GRAESSDRERESTLQLLSLVQHGQGARKAGKHTKATATAATTTAPPPPPAAPPAASQAAA TAAPPPPPDYHHHHQQHLLNSSNNGGSGGINGEQQPPASTPGDQRNSALIALQGSLKRKQ VVNLSPANSKRPNGFVDNSFLDIKRIRVGENLSAGQGGLQINNGQSQIMSGTLPMSQAPL RKTNTLPSHTHSPGNGLFNMGLKEVKKEPGETLSCSKHMDGQMTQENIFPNRYGDDPGEQ LMDPELQELFNELTNISVPPMSDLELENMINATIKQDDPFNIDLGQQSQRSTPRPSLPME KIVIKSEYSPGLTQGPSGSPQLRPPSAGPAFSMANSALSTSSPIPSVPQSQAQPQTGSGA SRALPSWQEVSHAQQLKQIAANRQQHARMQQHQQQHQPTNWSALPSSAGPSPGPFGQEKI PSPSFGQQTFSPQSSPMPGVAGGSGQSKVMANYMYKAGPSAQGGHLDVLMQQKPQDLSRS FINNPHPAMEPRQGNTKPLFHFNSDQANQQMPSVLPSQNKPSLLHYTQQQQQQQQQQQQQ QQQQQQQQQQQQQQQQQQSSISAQQQQQQQSSISAQQQQQQQQQQQQQQQQQQQQQQQQQ QQPSSQPAQSLPSQPLLRSPLPLQQKLLLQQMQNQPIAGMGYQVSQQQRQDQHSVVGQNT GPSPSPNPCSNPNTGSGYMNSQQSLLNQQLMGKKQTLQRQIMEQKQQLLLQQQMLADAEK IAPQDQINRHLSRPPPDYKDQRRNVGNMQPTAQYSGGSSTISLNSNQALANPVSTHTILT PNSSLLSTSHGTRMPSLSTAVQNMGMYGNLPCNQPNTYSVTSGMNQLTQQRNPKQLLANQ NNPMMPRPPTLGPSNNNNVATFGAGSVGNSQQLRPNLTHSMASMPPQRTSNVMITSNTTA PNWASQEGTSKQQEALTSAGVRFPTGTPAAYTPNQSLQQAVGSQQFSQRAVAPPNQLTPA VQMRPMNQMSQTLNGQTMGPLRGLNLRPNQLSTQILPNLNQSGTGLNQSRTGINQPPSLT PSNFPSPNQSSRAFQGTDHSSDLAFDFLSQQNDNMGPALNSDADFIDSLLKTEPGNDDWM KDINLDEILGNNS
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
