Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
FUSION
Source:
None
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00741014
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 2229nts
Open reading frame: 1 to 2229
ATGAACAATCCGTCAGAAACCAGTAAACCATCTATGGAGAGTGGAGATGGCAACACAGGC
ACACAAACCAATGGTCTGGACTTTCAGAAGCAGCCTGTGCCTGTAGGAGGAGCAATCTCA
ACAGCCCAGGCGCAGGCTTTCCTTGGACATCTCCATCAGGTCCAACTCGCTGGAACAAGT
TTACAGGCTGCTGCTCAGTCTTTAAATGTACAGTCTAAATCTAATGAAGAATCGGGGGAT
TCGCAGCAGCCAAGCCAGCCTTCCCAGCAGCCTTCAGTGCAGGCAGCCATTCCCCAGACC
CAGCTTATGCTAGCTGGAGGACAGATAACTGGGCTTACTTTGACGCCTGCCCAGCAACAG
TTACTACTCCAGCAGGCACAGGCACAGGCACAGCTGCTGGCTGCTGCAGTGCAGCAGCAC
TCCGCCAGCCAGCAGCACAGTGCTGCTGGAGCCACCATCTCCGCCTCTGCTGCCACGCCC
ATGACGCAGATCCCCCTGTCTCAGCCCATACAGATCGCACAGGATCTTCAACAACTGCAA
CAGCTTCAACAGCAGAATCTCAACCTGCAACAGTTTGTGTTGGTGCATCCAACCACCAAT
TTGCAGCCAGCGCAGTTTATCATCTCACAGACGCCCCAGGGCCAGCAGGGTCTCCTGCAA
GCGCAAAATCTTCTAACGCAACTACCTCAGCAAAGCCAAGCCAACCTCCTACAGTCGCAG
CCAAGCATCACCCTCACCTCCCAGCCAGCAACCCCAACACGCACAATAGCAGCAACCCCA
ATTCAGACACTTCCACAGAGCCAGTCAACACCAAAGCGAATTGATACTCCCAGCTTGGAG
GAGCCCAGTGACCTTGAGGAGCTTGAGCAGTTTGCCAAGACCTTCAAACAAAGACGAATC
AAACTTGGATTCACTCAGGGTGATGTTGGGCTCGCTATGGGGAAACTATATGGAAATGAC
TTCAGCCAAACTACCATCTCTCGATTTGAAGCCTTGAACCTCAGCTTTAAGAACATGTGC
AAGTTGAAGCCACTTTTAGAGAAGTGGCTAAATGATGCAGAGAACCTCTCATCTGATTCG
TCCCTCTCCAGCCCAAGTGCCCTGAATTCTCCAGGAATTGAGGGCTTGAGCCGTAGGAGG
AAGAAACGCACCAGCATAGAGACCAACATCCGTGTGGCCTTAGAGAAGAGTTTCTTGGAG
AATCAAAAGCCTACCTCGGAAGAGATCACTATGATTGCTGATCAGCTCAATATGGAAAAA
GAGGTGATTCGTGTTTGGTTCTGTAACCGCCGCCAGAAAGAAAAAAGAATCAACCCACCA
AGCAGTGGTGGGACCAGCAGCTCACCTATTAAAGCAATTTTCCCCAGCCCAACTTCACTG
GTGGCGACCACACCAAGCCTTGTGACTAGCAGTGCAGCAACTACCCTCACAGTCAGCCCT
GTCCTCCCTCTGACCAGTGCTGCTGTGACGAATCTTTCAGTTACAGGCACTTCAGACACC
ACCTCCAACAACACAGCAACCGTGATTTCCACAGCGCCTCCAGCTTCCTCAGCAGTCACG
TCCCCCTCTCTGAGTCCCTCCCCTTCTGCCTCAGCCTCCACCTCCGAGGCATCCAGTGCC
AGTGAGACCAGCACAACACAGACCACCTCCACTCCTTTGTCCTCCCCTCTTGGGACCAGC
CAGGTGATGGTGACAGCATCAGGTTTGCAAACAGCAGCAGCTGCTGCCCTTCAAGGAGCT
GCACAGTTGCCAGCAAATGCCAGTCTTGCTGCCATGGCAGCTGCTGCAGGACTAAACCCA
AGCCTGATGGCACCCTCACAGTTTGCGGCTGGAGGTGCCTTACTCAGTCTGAATCCAGGG
ACCCTGAGCGGTGCTCTCAGCCCAGCTCTAATGAGCAACAGTACACTGGCAACTATTCAA
GCTCTTGCTTCTGGTGGCTCTCTTCCAATAACATCACTTGATGCAACTGGGAACCTGGTA
TTTGCCAATGCGGGAGGAGCCCCCAACATCGTGACTGCCCCTCTGTTCCTGAACCCTCAG
AACCTCTCTCTGCTCACCAGCAACCCTGTTAGCTTGGTCTCTGCCGCCGCAGCATCTGCA
GGGAACTCTGCACCTGTAGCCAGCCTTCACGCCACCTCCACCTCTGCTGAGTCCATCCAG
AACTCTCTCTTCACAGTGGCCTCTGCCAGCGGGGCTGCGTCCACCACCACCACCGCCTCC
AAGGCACAG
Protein sequence predicted based on nucleotide sequence
MNNPSETSKPSMESGDGNTGTQTNGLDFQKQPVPVGGAISTAQAQAFLGHLHQVQLAGTS LQAAAQSLNVQSKSNEESGDSQQPSQPSQQPSVQAAIPQTQLMLAGGQITGLTLTPAQQQ LLLQQAQAQAQLLAAAVQQHSASQQHSAAGATISASAATPMTQIPLSQPIQIAQDLQQLQ QLQQQNLNLQQFVLVHPTTNLQPAQFIISQTPQGQQGLLQAQNLLTQLPQQSQANLLQSQ PSITLTSQPATPTRTIAATPIQTLPQSQSTPKRIDTPSLEEPSDLEELEQFAKTFKQRRI KLGFTQGDVGLAMGKLYGNDFSQTTISRFEALNLSFKNMCKLKPLLEKWLNDAENLSSDS SLSSPSALNSPGIEGLSRRRKKRTSIETNIRVALEKSFLENQKPTSEEITMIADQLNMEK EVIRVWFCNRRQKEKRINPPSSGGTSSSPIKAIFPSPTSLVATTPSLVTSSAATTLTVSP VLPLTSAAVTNLSVTGTSDTTSNNTATVISTAPPASSAVTSPSLSPSPSASASTSEASSA SETSTTQTTSTPLSSPLGTSQVMVTASGLQTAAAAALQGAAQLPANASLAAMAAAAGLNP SLMAPSQFAAGGALLSLNPGTLSGALSPALMSNSTLATIQALASGGSLPITSLDATGNLV FANAGGAPNIVTAPLFLNPQNLSLLTSNPVSLVSAAAASAGNSAPVASLHATSTSAESIQ NSLFTVASASGAASTTTTASKAQ
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
