Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
CLOSED (STOP codon in vector)
Source:
None
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00741169
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 5583nts
Open reading frame: 1 to 5583
GCCTCCTCCGCCTCCTCCTCTCCCGCGGGCACCGAGGACTCCGCTCCTGCCCAGGGTGGC
TTTGGATCAGATTATTCACGAAGCTCTAGAAAGTCTGATGCCAATGCAAGTTACTTAAGA
GCAGCTCGAGCTGGACACCTTGAAAAGGCCCTCGACTACATAAAAAATGGAGTTGACATC
AACATTTGCAATCAGAATGGGTTGAACGCTCTCCACCTTGCTTCCAAAGAAGGCCATGTA
GAGGTTGTTTCTGAGCTGCTGCAGAGAGAAGCCAATGTGGATGCAGCTACAAAGAAAGGA
AACACAGCATTGCACATCGCATCTTTGGCTGGGCAAGCAGAGGTGGTAAAAGTCTTGGTT
ACAAATGGAGCCAATGTCAATGCACAATCTCAGAATGGTTTCACGCCATTGTATATGGCA
GCCCAGGAAAATCACCTGGAAGTTGTCAAGTTTCTTCTTGACAATGGTGCAAGCCAGAGC
CTAGCCACAGAGGATGGCTTCACACCATTGGCAGTGGCTTTGCAACAAGGTCACGACCAA
GTCGTTTCGCTCCTGCTAGAGAATGACACCAAAGGAAAAGTGCGTCTCCCAGCTCTTCAT
ATCGCGGCCCGAAAAGACGACACGAAAGCCGCCGCCCTGCTGCCGCAGAATGACAACAAT
GCAGATGTGGAATCAAAGAGTGGCTTCACTCCGCTCCACATAGCTGCTCACTATGGAAAT
ATCAATGTAGCCACGTTGCTGTTAAACCGAGCGGCTGCTGTGGATTTCACCGCAAGGAAT
GACATCACTCCTTTACATGTTGCATCAAAAAGAGGAAATGCAAATATGGTAAAACTATTG
CTCGATCGAGGAGCTAAAATCGATGCCAAAACCAGGGATGGTCTGACACCACTGCACTGT
GGAGCAAGGAGTGGCCACGAGCAGGTGGTAGAAATGTTGCTTGATCGAGCTGCCCCCGTT
CTTTCAAAAACCAAGAATGGATTATCTCCATTGCACATGGCCACACAAGGGGATCATTTA
AACTGCGTCCAGCTTCTCCTCCAGCATAATGTACCCGTGGATGATGTCACCAATGACTAC
CTGACTGCCCTACACGTGGCTGCCCACTGTGGCCATTACAAAGTTGCCAAGGTTCTCTTG
GATAAGAAAGCTAACCCCAATGCCAAAGCCCTGAATGGCTTTACCCCTCTTCATATTGCC
TGCAAGAAGAATCGAATTAAAGTAATGGAACTCCTTCTGAAACACGGTGCATCCATCCAA
GCTGTAACCGAGTCGGGCCTTACCCCAATCCATGTTGCTGCCTTCATGGGGCATGTAAAT
ATTGTATCACAACTAATGCATCATGGAGCCTCACCAAACACCACCAATGTGAGAGGAGAA
ACAGCACTGCACATGGCAGCTCGCTCCGGCCAAGCTGAAGTTGTGCGGTATCTGGTACAA
GACGGAGCTCAGGTAGAAGCTAAAGCTAAGGATGACCAAACACCACTCCACATTTCAGCC
CGACTGGGGAAAGCAGACATAGTACAACAGCTGTTGCAGCAAGGGACATCTCCAAATGCA
GCCACAACTTCTGGGTACACCCCACTTCACCTTTCCGCCCGAGAGGGGCATGAGGATGTG
GCCGCGTTCCTTTTGGATCATGGAGCGTCTTTATCTATAACAACAAAGAAAGGATTTACT
CCTCTTCATGTGGCAGCAAAATATGGAAAGCTTGAAGTCGCCAATCTCCTGCTACAGAAA
AGTGCATCTCCAGATGCTGCTGGGAAGAGCGGGCTAACACCACTGCATGTAGCTGCACAT
TACGATAATCAGAAAGTGGCCCTTCTGCTTTTGGACCAAGGAGCCTCACCTCACGCAGCC
GCAAAGAATGGTTATACGCCACTGCACATCGCTGCCAAAAAGAACCAGATGGACATAGCG
ACAACTCTGCTGGAATATGGTGCTGATGCCAACGCAGTTACCCGGCAAGGAATTGCTTCC
GTCCATCTCGCAGCTCAGGAAGGGCACGTGGACATGGTGTCGCTGCTCCTCGGTAGAAAT
GCGAATGTGAACCTGAGCAATAAGAGCGGCCTGACCCCACTCCATTTGGCTGCTCAAGAA
GATCGAGTGAATGTGGCAGAAGTCCTCGTAAACCAAGGGGCTCATGTGGACGCCCAGACA
AAGATGGGATACACACCACTGCATGTGGGCTGCCACTATGGAAATATCAAGATTGTTAAT
TTCCTGCTCCAGCATTCTGCAAAAGTTAATGCCAAAACAAAGAATGGGTATACGCCATTA
CATCAAGCAGCACAGCAGGGGCATACGCATATAATAAATGTCTTACTTCAGAACAACGCC
TCCCCCAATGAACTCACTGTGAATGGGAATACTGCCCTTGGCATTGCCCGGCGCCTCGGC
TACATCTCAGTAGTGGACACCCTGAAGATAGTGACCGAAGAGACCATGACCACAACTACT
GTCACAGAGAAGCACAAAATGAATGTTCCAGAAACGATGAATGAAGTTCTTGATATGTCT
GATGATGAAGTTCGTAAAGCCAATGCCCCTGAAATGCTCAGTGATGGCGAATATATCTCA
GATGTTGAAGAAGGTGAAGATGCAATGACCGGGGACACAGACAAATATCTTGGGCCACAG
GACCTTAAGGAATTGGGTGATGATTCCCTGCCTGCAGAGGGTTACATGGGCTTTAGTCTC
GGAGCGCGTTCTGCCAGCCTCCGCTCCTTCAGTTCGGATAGGTCTTACACCTTGAACAGA
AGCTCCTATGCACGGGACAGCATGATGATTGAAGAACTCCTTGTGCCATCCAAAGAGCAG
CATCTAACATTCACAAGGGAATTTGATTCAGATTCTCTTAGACATTACAGCTGGGCTGCA
GACACCTTAGACAATGTCAATCTTGTTTCAAGCCCCATTCATTCTGGGTTTCTGGTTAGC
TTTATGGTGGACGCGAGAGGGGGCTCCATGAGAGGAAGCCGTCATCACGGGATGAGAATC
ATCATTCCTCCACGCAAGTGTACGGCCCCCACTCGAATCACCTGCCGTTTGGTAAAGAGA
CATAAACTGGCCAACCCACCCCCCATGGTGGAAGGAGAGGGATTAGCCAGTAGGCTGGTA
GAAATGGGTCCTGCAGGGGCACAATTTTTAGGCCCTGTCATAGTGGAAATCCCTCACTTT
GGGTCCATGAGAGGAAAAGAGAGAGAACTCATTGTTCTTCGAAGTGAAAATGGTGAAACT
TGGAAGGAGCATCAGTTTGACAGCAAAAATGAAGATTTAACCGAGTTACTTAATGGCATG
GATGAAGAACTTGATAGCCCAGAAGAGTTAGGGAAAAAGCGTATCTGCAGGATTATCACG
AAAGATTTCCCCCAGTATTTTGCAGTGGTTTCCCGGATTAAGCAGGAAAGCAACCAGATT
GGTCCTGAAGGTGGAATTCTGAGCAGCACCACAGTGCCCCTTGTTCAAGCATCTTTCCCA
GAGGGTGCCCTAACTAAAAGAATTCGAGTGGGCCTCCAGGCCCAGCCTGTTCCAGATGAA
ATTGTGAAAAAGATCCTTGGAAACAAAGCAACTTTTAGCCCAATTGTCACTGTGGAACCA
AGAAGACGGAAATTCCATAAACCAATCACAATGACCATTCCGGTGCCCCCGCCCTCAGGA
GAAGGTGTATCCAATGGATACAAAGGGGACACTACACCCAATCTGCGTCTTCTCTGTAGC
ATTACAGGGGGCACTTCGCCTGCTCAGTGGGAAGACATCACAGGAACAACTCCTTTGACG
TTTATAAAAGATTGTGTCTCCTTTACAACCAATGTTTCAGCCAGATTTTGGCTTGCAGAC
TGCCATCAAGTTTTAGAAACTGTGGGGTTAGCCACGCAACTGTACAGAGAATTGATATGT
GTTCCATATATGGCCAAGTTTGTTGTTTTTGCCAAAATGAATGATCCCGTAGAATCTTCC
TTGCGATGTTTCTGCATGACAGATGACAAAGTGGACAAAACTTTAGAGCAACAAGAGAAT
TTTGAGGAAGTCGCAAGAAGCAAAGATATTGAGGTTCTGGAAGGAAAACCTATTTATGTT
GATTGTTATGGAAATTTGGCCCCACTTACCAAAGGAGGACAGCAACTTGTTTTTAACTTT
TATTCTTTCAAAGAAAATAGACTGCCATTTTCCATCAAGATTAGAGACACCAGCCAAGAG
CCCTGTGGTCGTCTGTCTTTTCTGAAAGAACCAAAGACAACAAAAGGACTGCCTCAAACA
GCGGTTTGCAACTTAAATATCACTCTGCCAGCACATAAAAAGATTGAGAAAACAGATAGA
CGACAGAGCTTCGCATCCTTAGCTTTACGTAAGCGCTACAGCTACTTGACTGAGCCTGGA
ATGAGTCCACAGAGTCCATGTGAACGGACAGATATCAGGATGGCAATAGTAGCCGATCAC
CTGGGACTTAGTTGGACAGAACTGGCAAGGGAACTGAATTTTTCAGTGGATGAAATCAAT
CAAATACGTGTGGAAAATCCAAATTCTTTAATTTCTCAGAGCTTCATGTTATTAAAAAAA
TGGGTTACCAGAGACGGAAAAAATGCCACAACTGATGCCTTAACTTCGGTCTTGACAAAA
ATTAATCGAATAGATATAGTGACACTGCTAGAAGGACCAATATTTGATTATGGAAATATT
TCAGGCACCAGAAGTTTTGCAGATGAGAACAATGTTTTCCATGACCCTGTTGATGGTTAT
CCTTCCCTTCAAGTGGAACTGGAAACCCCCACAGGGTTGCACTACACACCACCTACCCCT
TTCCAGCAAGATGATTATTTTAGTGATATCTCTAGCATAGAATCTCCCCTTAGAACCCCT
AGTAGACTGAGTGATGGGCTAGTGCCTTCCCAGGGGAACATAGAGCATTCCGCAGATGGA
CCTCCAGTCGTAACTGCAGAAGACGCTTCCTTAGAAGACAGCAAACTGGAAGACTCAGTG
CCTTTAACAGAAATGCCTGAAGCAGTGGATGTAGATGAGAGCCAGTTGGAGAATGTATGT
CTGAGTTGGCAGAATGAGACATCAAGTGGAAACCTAGAGTCCTGCGCTCAAGCTCGAAGA
GTAACTGGTGGGTTACTAGATCGACTGGATGACAGCCCTGACCAGTGTAGAGATTCCATT
ACCTCATATCTCAAAGGAGAAGCTGGCAAATTTGAAGCAAATGGAAGCCATACAGAAATC
ACTCCAGAAGCAAAGACAAAATCTTACTTTCCAGAATCCCAAAATGATGTAGGAAAACAG
AGTACCAAGGAAACTCTGAAACCAAAAATACATGGATCTGGTCATGTTGAAGAACCAGCA
TCACCACTAGCAGCATATCAGAAATCTCTAGAAGAAACCAGCAAGCTTATAATAGAAGAG
ACTAAACCCTGTGTGCCTGTCAGTATGAAAAAGATGAGTAGGACTTCTCCAGCAGATGGC
AAGCCAAGGCTTAGCCTCCATGAAGAAGAGGGGTCCAGTGGGTCTGAGCAAAAGCAGGGA
GAAGGTTTTAAGGTGAAAACGAAGAAAGAAATCCGGCATGTGGAAAAGAAGAGCCACTCG
TGG
Protein sequence predicted based on nucleotide sequence
ASSASSSPAGTEDSAPAQGGFGSDYSRSSRKSDANASYLRAARAGHLEKALDYIKNGVDI NICNQNGLNALHLASKEGHVEVVSELLQREANVDAATKKGNTALHIASLAGQAEVVKVLV TNGANVNAQSQNGFTPLYMAAQENHLEVVKFLLDNGASQSLATEDGFTPLAVALQQGHDQ VVSLLLENDTKGKVRLPALHIAARKDDTKAAALLPQNDNNADVESKSGFTPLHIAAHYGN INVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHC GARSGHEQVVEMLLDRAAPVLSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDY LTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRIKVMELLLKHGASIQ AVTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLVQ DGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQGTSPNAATTSGYTPLHLSAREGHEDV AAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVANLLLQKSASPDAAGKSGLTPLHVAAH YDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIATTLLEYGADANAVTRQGIAS VHLAAQEGHVDMVSLLLGRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQT KMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTPLHQAAQQGHTHIINVLLQNNA SPNELTVNGNTALGIARRLGYISVVDTLKIVTEETMTTTTVTEKHKMNVPETMNEVLDMS DDEVRKANAPEMLSDGEYISDVEEGEDAMTGDTDKYLGPQDLKELGDDSLPAEGYMGFSL GARSASLRSFSSDRSYTLNRSSYARDSMMIEELLVPSKEQHLTFTREFDSDSLRHYSWAA DTLDNVNLVSSPIHSGFLVSFMVDARGGSMRGSRHHGMRIIIPPRKCTAPTRITCRLVKR HKLANPPPMVEGEGLASRLVEMGPAGAQFLGPVIVEIPHFGSMRGKERELIVLRSENGET WKEHQFDSKNEDLTELLNGMDEELDSPEELGKKRICRIITKDFPQYFAVVSRIKQESNQI GPEGGILSSTTVPLVQASFPEGALTKRIRVGLQAQPVPDEIVKKILGNKATFSPIVTVEP RRRKFHKPITMTIPVPPPSGEGVSNGYKGDTTPNLRLLCSITGGTSPAQWEDITGTTPLT FIKDCVSFTTNVSARFWLADCHQVLETVGLATQLYRELICVPYMAKFVVFAKMNDPVESS LRCFCMTDDKVDKTLEQQENFEEVARSKDIEVLEGKPIYVDCYGNLAPLTKGGQQLVFNF YSFKENRLPFSIKIRDTSQEPCGRLSFLKEPKTTKGLPQTAVCNLNITLPAHKKIEKTDR RQSFASLALRKRYSYLTEPGMSPQSPCERTDIRMAIVADHLGLSWTELARELNFSVDEIN QIRVENPNSLISQSFMLLKKWVTRDGKNATTDALTSVLTKINRIDIVTLLEGPIFDYGNI SGTRSFADENNVFHDPVDGYPSLQVELETPTGLHYTPPTPFQQDDYFSDISSIESPLRTP SRLSDGLVPSQGNIEHSADGPPVVTAEDASLEDSKLEDSVPLTEMPEAVDVDESQLENVC LSWQNETSSGNLESCAQARRVTGGLLDRLDDSPDQCRDSITSYLKGEAGKFEANGSHTEI TPEAKTKSYFPESQNDVGKQSTKETLKPKIHGSGHVEEPASPLAAYQKSLEETSKLIIEE TKPCVPVSMKKMSRTSPADGKPRLSLHEEEGSSGSEQKQGEGFKVKTKKEIRHVEKKSHS W
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
