Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
CLOSED (STOP codon in vector)
Source:
None
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00741353
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 2610nts
Open reading frame: 1 to 2610
AACCACCTGAACGTGCTGGCCAAAGCGCTCTATGACAATGTGGCCGAGTCCCCGGATGAG
CTCTCCTTCCGCAAGGGTGACATCATGACGGTGCTGGAGCAGGACACGCAGGGCCTGGAC
GGCTGGTGGCTCTGCTCGCTGCATGGGCGCCAGGGCATCGTGCCTGGGAACCGCCTCAAG
ATCTTGGTGGGCATGTATGATAAGAAGCCAGCAGGGCCTGGCTCCGGCCCTCCCGCCACC
CCGGCCCAGCCTCAGCCTGGCCTCCATGCCCCAGCGCCTCCGGCCTCCCAGTACACGCCC
ATGCTCCCCAACACCTACCAGCCCCAGCCAGACAGCGTCTACCTGGTGCCCACTCCCAGC
AAGGCTCAGCAAGGCCTCTACCAAGTCCCGGGTCCCAGCCCTCAGTTCCAGTCTCCCCCA
GCCAAGCAGACATCCACCTTCTCGAAGCAGACACCCCATCACCCGTTTCCCAACCCGGCC
ACAGACCTGTACCAGGTGCCCCCAGGGCCTGGAGGCCCTGCCCAGGATATTTACCAGGTG
CCACCTTCTGCCGGGATGGGGCATGACATCTACCAGGTCCCCCCGTCCATGGACACACGC
AGCTGGGAGGGCACGAAGCCCCCGGCAAAGGTGGTGGTGCCCACCCGCGTGGGGCAGGGC
TATGTATACGAGGCCGCCCAGCCGGAGCAGGACGAGTACGACATCCCGCGACACCTGCTG
GCCCCGGGGCCACAGGACATCTATGATGTGCCCCCGGTTCGGGGGCTGCTTCCCAGCCAG
TATGGCCAGGAGGTGTATGACACACCCCCCATGGCTGTCAAGGGTCCCAATGGCCGAGAC
CCGTTGCTGGAGGTGTATGACGTGCCCCCCAGTGTGGAGAAGGGCCTGCCACCGTCCAAC
CACCACGCAGTCTACGACGTTCCTCCATCGGTGAGCAAGGATGTGCCCGATGGCCCACTG
CTGCGTGAGGAGACCTACGATGTGCCCCCCGCCTTCGCCAAGGCCAAGCCCTTTGACCCG
GCCCGCACCCCACTGGTACTGGCTGCGCCCCCTCCAGACTCCCCGCCGGCCGAGGACGTG
TATGACGTGCCGCCCCCGGCTCCTGACCTCTACGACGTGCCCCCTGGCTTGCGGCGGCCT
GGCCCGGGCACCCTGTACGATGTGCCCCGTGAACGGGTGCTTCCTCCTGAGGTGGCTGAT
GGTGGCGTGGTCGACAGTGGTGTGTATGCGGTGCCTCCCCCAGCTGAACGTGAAGCCCCG
GCAGAGGGCAAGCGCCTGTCGGCCTCCAGCACCGGCAGCACACGCAGCAGCCAGTCTGCG
TCCTCCTTGGAGGTGGCAGGGCCGGGCCGGGAACCCCTGGAGCTGGAAGTTGCTGTGGAG
GCCCTGGCACGGCTGCAGCAGGGTGTGAGCGCCACCGTTGCCCACCTTCTGGACCTGGCA
GGCAGCGCCGGTGCGACTGGGAGCTGGCGTAGCCCCTCTGAGCCACAGGAGCCGCTGGTG
CAGGACCTGCAGGCTGCTGTGGCCGCCGTCCAGAGTGCCGTCCACGAGCTGTTGGAGTTT
GCCCGCAGCGCGGTGGGCAATGCTGCCCACACATCTGACCGTGCCCTGCATGCCAAGCTT
AGCCGGCAGCTGCAGAAGATGGAGGACGTGCACCAGACGCTGGTGGCACATGGTCAGGCC
CTCGACGCTGGCCGGGGAGGCTCTGGAGCCACCCTTGAGGACCTGGACCGGCTGGTGGCC
TGCTCGCGGGCTGTGCCCGAGGACGCCAAGCAGCTGGCCTCCTTCCTGCACGGCAATGCC
TCACTGCTCTTCAGACGGACCAAGGCCACTGCCCCGGGGCCTGAGGGGGGTGGCACCCTG
CACCCCAACCCCACTGACAAGACCAGCAGCATCCAGTCACGACCCCTGCCCTCACCCCCT
AAGTTCACCTCCCAGGACTCGCCAGATGGGCAGTACGAGAACAGCGAGGGGGGCTGGATG
GAGGACTATGACTACGTCCACCTACAGGGGAAGGAGGAGTTTGAGAAGACCCAGAAGGAG
CTGCTGGAAAAGGGCAGCATCACGCGGCAGGGCAAGAGCCAGCTGGAGTTGCAGCAGCTG
AAGCAGTTTGAACGACTGGAACAGGAGGTGTCACGGCCCATAGACCACGACCTGGCCAAC
TGGACGCCAGCCCAACCCCTGGCCCCGGGGCGAACAGGCGGCCTGGGGCCCTCGGACCGG
CAGCTGCTGCTCTTCTACCTGGAGCAGTGTGAGGCCAACCTGACCACACTGACCAACGCC
GTGGACGCCTTCTTTACCGCCGTGGCCACCAACCAGCCGCCCAAGATCTTTGTGGCGCAC
AGCAAGTTCGTCATCCTCAGCGCCCACAAGCTGGTGTTCATCGGGGACACACTGTCACGG
CAGGCCAAGGCTGCTGACGTGCGCAGCCAGGTGACCCACTACAGCAACCTGCTGTGCGAC
CTCCTGCGCGGCATCGTGGCCACCACCAAGGCCGCTGCCTTGCAGTACCCATCGCCTTCC
GCGGCCCAGGACATGGTGGAGAGGGTCAAGGAGCTGGGCCACAGCACCCAGCAGTTCCGC
CGCGTCCTAGGCCAGCTGGCAGCCGCCTGG
Protein sequence predicted based on nucleotide sequence
NHLNVLAKALYDNVAESPDELSFRKGDIMTVLEQDTQGLDGWWLCSLHGRQGIVPGNRLK ILVGMYDKKPAGPGSGPPATPAQPQPGLHAPAPPASQYTPMLPNTYQPQPDSVYLVPTPS KAQQGLYQVPGPSPQFQSPPAKQTSTFSKQTPHHPFPNPATDLYQVPPGPGGPAQDIYQV PPSAGMGHDIYQVPPSMDTRSWEGTKPPAKVVVPTRVGQGYVYEAAQPEQDEYDIPRHLL APGPQDIYDVPPVRGLLPSQYGQEVYDTPPMAVKGPNGRDPLLEVYDVPPSVEKGLPPSN HHAVYDVPPSVSKDVPDGPLLREETYDVPPAFAKAKPFDPARTPLVLAAPPPDSPPAEDV YDVPPPAPDLYDVPPGLRRPGPGTLYDVPRERVLPPEVADGGVVDSGVYAVPPPAEREAP AEGKRLSASSTGSTRSSQSASSLEVAGPGREPLELEVAVEALARLQQGVSATVAHLLDLA GSAGATGSWRSPSEPQEPLVQDLQAAVAAVQSAVHELLEFARSAVGNAAHTSDRALHAKL SRQLQKMEDVHQTLVAHGQALDAGRGGSGATLEDLDRLVACSRAVPEDAKQLASFLHGNA SLLFRRTKATAPGPEGGGTLHPNPTDKTSSIQSRPLPSPPKFTSQDSPDGQYENSEGGWM EDYDYVHLQGKEEFEKTQKELLEKGSITRQGKSQLELQQLKQFERLEQEVSRPIDHDLAN WTPAQPLAPGRTGGLGPSDRQLLLFYLEQCEANLTTLTNAVDAFFTAVATNQPPKIFVAH SKFVILSAHKLVFIGDTLSRQAKAADVRSQVTHYSNLLCDLLRGIVATTKAAALQYPSPS AAQDMVERVKELGHSTQQFRRVLGQLAAAW
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
