Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
CLOSED (STOP codon in vector)
Source:
None
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00741380
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 3558nts
Open reading frame: 1 to 3558
GACGAGCCTCCCGGGAAGCCCCTCAGCTGTGAGGAGAAGGAAAAGTTAAAGGAGAAATTA
GCATTCTTGAAAAGGGAATACAGCAAGACACTAGCCCGCCTTCAGCGTGCCCAAAGAGCT
GAAAAGATTAAGCATTCTATTAAGAAAACAGTAGAAGAACAAGATTGTTTGTCTCAGCAG
GATCTCTCACCGCAGCTAAAACACTCAGAACCTAAAAATAAAATATGTGTTTATGACAAG
TTACACATCAAAACCCATCTTGATGAAGAAACTGGAGAAAAGACATCTATCACACTTGAT
GTTGGGCCTGAGTCCTTTAACCCTGGAGATGGCCCAGGAGGATTACCTATACAAAGAACA
GATGACACCCAAGAACATTTTCCCCACAGGGTCAGTGACCCTAGTGGTGAGCAAAAGCAG
AAGCTGCCAAGCAGAAGAAAGAAGCAGCAGAAGAGGACATTTATTTCACAGGAGAGAGAC
TGTGTCTTTGGCACTGATTCACTCAGATTGTCTGGGAAAAGACTAAAGGAACAGGAAGAA
ATCAGTAGCAAAAATCCTGCTAGATCACCAGTAACTGAAATAAGAACTCACCTTTTAAGT
CTTAAATCTGAACTTCCAGATTCTCCAGAACCAGTTACAGAAATTAATGAAGACAGTGTA
TTAATTCCACCAACTGCCCAACCAGAAAAAGGTGTTGATACATTCCTAAGAAGACCTAAT
TTCACCAGGGCGACTACAGTTCCTTTACAGACTCTATCAGATAGCGGTAGTAGTCAGCAC
CTTGAACACATTCCTCCTAAAGGTAGCAGTGAACTTACTACTCACGACCTAAAAAACATT
AGATTTACTTCACCTGTAAGTTTGGAGGCACAAGGCAAAAAAATGACTGTCTCTACAGAT
AACCTCCTTGTAAATAAAGCTATAAGTAAAAGTGGCCAACTGCCCACAAGTTCTAATTTA
GAGGCAAATATTTCATGTTCTCTAAATGAACTCGCCTACAATAACTTACCAGCAAATGAA
AACCAAAACTTAAAAGAACAAAATCAAACAGAGAAATCTTTAAAATCTCCCAGTGACACT
CTTGATGGCAGGAATGAAAATCTTCAGGAAAGTGAGATTCTAAGTCAACCTAAGAGTCTT
AGCCTGGAAGCAACCTCTCCTCTTTCTGCAGAAAAACATTCTTGCACAGTGCCTGAAGGC
CTTCTGTTTCCTGCAGAATATTATGTTAGAACAACACGAAGCATGTCCAATTGCCAGAGG
AAAGTAGCCGTGGAGGCTGTCATTCAGAGTCATTTGGATGTCAAGAAAAAAGGGTTTAAA
AATAAAAATAAGGATGCAAGTAAAAATTTAAACCTTTCCAATGAGGAAACTGACCAAAGT
GAAATTAGGATGTCTGGCACATGCACAGGACAACCAAGTTCAAGAACCTCTCAGAAACTT
CTCTCATTAACTAAAGTCAGCTCTCCCGCTGGGCCCACTGAAGATAATGACTTGTCTAGG
AAGGCAGTTGCCCAAGCACCTGGTAGAAGATACACAGGAAAAAGAAAATCAGCCTGCACC
CCAGCATCAGATCATTGTGAACCACTTTTGCCAACTTCTAGCCTGTCGATTGTTAACAGG
TCCAAGGAAGAAGTCACCTCACACAAATATCAGCACGAAAAATTATTTATTCAAGTGAAA
GGGAAGAAAAGTCGTCATCAAAAAGAGGATTCCCTTTCTTGGAGTAATAGTGCTTATTTA
TCCTTGGATGATGATGCTTTCACGGCTCCATTTCATAGGGATGGAATGCTGAGTTTAAAG
CAACTACTGTCTTTTCTCAGTATCACAGACTTTCAGTTACCTGATGAAGACTTTGGACCT
CTTAAGCTTGAAAAAGTGAAGTCCTGCTCAGAAAAACCAGTGGAGCCCTTTGAGTCAAAA
ATGTTTGGAGAGAGACATCTTAAAGAGGGAAGCTGTATTTTTCCAGAGGAACTGAGTCCT
AAACGCATGGATACAGAAATGGAGGACTTAGAAGAGGACCTTATTGTTCTACCAGGAAAA
TCACATCCCAAAAGGCCAAACTCGCAAAGCCAGCATACAAAGACGGGCCTTTCTTCATCC
ATACTACTTTATACTCCTTTAAATACGGTTGCGCCTGATGATAATGACAGGCCTACCACA
GACATGTGTTCACCTGCTTTCCCCATCTTAGGTACTACTCCAGCCTTTGGCCCTCAAGGC
TCCTATGAAAAAGCATCTACAGAAGTTGCTGGACGAACTTGCTGCACACCCCAACTTGCT
CATTTGAAAGACTCAGTCTGTCTTGCCAGTGATACTAAACAATTCGACAGTTCAGGCAGC
CCAGCAAAACCACATACCACCCTGCAAGTGTCAGGCAGGCAAGGACAACCTACCTGTGAC
TGTGACTCTGTCCCGCCAGGAACACCTCCACCCATTGAGTCATTCACTTTTAAAGAAAAT
CAGCTCTGTAGAAACACATGCCAGGAGCTGCATAAACATTCCGTCGAACAGACTGAAACA
GCAGAGCTTCCTGCTTCTGATAGCATAAACCCAGGCAACCTACAATTGGTTTCAGAGTTA
AAGAATCCTTCAGGTTCCTGTTCCGTAGATGTGAGTGCCATGTTTTGGGAAAGAGCCGGT
TGTAAAGAGCCATGTATCATAACTGCTTGCGAAGATGTAGTTTCTCTTTGGAAAGCTCTG
GATGCTTGGCAGTGGGAAAAACTTTATACCTGGCACTTCGCAGAGGTTCCAGTATTACAG
ATAGTTCCAGTGCCTGATGTGTATAATCTCGTGTGTGTAGCTTTGGGAAATTTGGAAATC
AGAGAGATCAGGGCATTGTTTTGTTCCTCTGATGATGAAAGTGAAAAGCAAGTACTACTG
AAGTCTGGAAATATAAAAGCTGTGCTTGGCCTGACAAAGAGGAGGCTAGTTAGTAGCAGT
GGGACCCTTTCTGATCAACAAGTAGAAGTCATGACGTTTGCAGAAGATGGAGGAGGCAAA
GAAAACCAATTTTTGATGCCCCCTGAGGAGACTATACTAACTTTTGCTGAGGTCCAAGGG
ATGCAAGAAGCTCTGCTTGGTACTACTATTATGAACAACATTGTTATTTGGAATTTAAAA
ACTGGTCAACTCCTGAAAAAGATGCACATTGATGATTCTTACCAAGCTTCAGTCTGTCAC
AAAGCCTATTCTGAAATGGGGCTTCTCTTTATTGTCCTGAGTCATCCCTGTGCCAAAGAG
AGTGAGTCGTTGCGAAGCCCTGTGTTTCAGCTCATTGTGATTAACCCTAAGACGACTCTC
AGCGTGGGTGTGATGCTGTACTGTCTTCCTCCAGGGCAGGCTGGCAGGTTCCTGGAAGGT
GACGTGAAAGATCACTGTGCAGCAGCAATCTTGACTTCTGGAACAATTGCCATTTGGGAC
TTACTTCTCGGTCAGTGTACTGCCCTCCTCCCACCTGTCTCTGACCAACATTGGTCTTTT
GTGAAATGGTCGGGTACAGACTCTCATTTGCTGGCTGGACAAAAAGATGGAAATATATTT
GTATACCGCTATTCAAAG
Protein sequence predicted based on nucleotide sequence
DEPPGKPLSCEEKEKLKEKLAFLKREYSKTLARLQRAQRAEKIKHSIKKTVEEQDCLSQQ DLSPQLKHSEPKNKICVYDKLHIKTHLDEETGEKTSITLDVGPESFNPGDGPGGLPIQRT DDTQEHFPHRVSDPSGEQKQKLPSRRKKQQKRTFISQERDCVFGTDSLRLSGKRLKEQEE ISSKNPARSPVTEIRTHLLSLKSELPDSPEPVTEINEDSVLIPPTAQPEKGVDTFLRRPN FTRATTVPLQTLSDSGSSQHLEHIPPKGSSELTTHDLKNIRFTSPVSLEAQGKKMTVSTD NLLVNKAISKSGQLPTSSNLEANISCSLNELAYNNLPANENQNLKEQNQTEKSLKSPSDT LDGRNENLQESEILSQPKSLSLEATSPLSAEKHSCTVPEGLLFPAEYYVRTTRSMSNCQR KVAVEAVIQSHLDVKKKGFKNKNKDASKNLNLSNEETDQSEIRMSGTCTGQPSSRTSQKL LSLTKVSSPAGPTEDNDLSRKAVAQAPGRRYTGKRKSACTPASDHCEPLLPTSSLSIVNR SKEEVTSHKYQHEKLFIQVKGKKSRHQKEDSLSWSNSAYLSLDDDAFTAPFHRDGMLSLK QLLSFLSITDFQLPDEDFGPLKLEKVKSCSEKPVEPFESKMFGERHLKEGSCIFPEELSP KRMDTEMEDLEEDLIVLPGKSHPKRPNSQSQHTKTGLSSSILLYTPLNTVAPDDNDRPTT DMCSPAFPILGTTPAFGPQGSYEKASTEVAGRTCCTPQLAHLKDSVCLASDTKQFDSSGS PAKPHTTLQVSGRQGQPTCDCDSVPPGTPPPIESFTFKENQLCRNTCQELHKHSVEQTET AELPASDSINPGNLQLVSELKNPSGSCSVDVSAMFWERAGCKEPCIITACEDVVSLWKAL DAWQWEKLYTWHFAEVPVLQIVPVPDVYNLVCVALGNLEIREIRALFCSSDDESEKQVLL KSGNIKAVLGLTKRRLVSSSGTLSDQQVEVMTFAEDGGGKENQFLMPPEETILTFAEVQG MQEALLGTTIMNNIVIWNLKTGQLLKKMHIDDSYQASVCHKAYSEMGLLFIVLSHPCAKE SESLRSPVFQLIVINPKTTLSVGVMLYCLPPGQAGRFLEGDVKDHCAAAILTSGTIAIWD LLLGQCTALLPPVSDQHWSFVKWSGTDSHLLAGQKDGNIFVYRYSK
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
