Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
FUSION
Source:
None
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00741862
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 4170nts
Open reading frame: 1 to 4170
ATGCCATCAGGTTCTTTCATGCTGGTGAATGCCTCTGGGAGACCTGAGGGGCAGAGAGCC
CACCTGCTCTTACCCCAACTTAAAGAAAATGACACCCACTGCATCGATTTTCACTATTTT
GTGTCCAGCAAGAGTAATTCTCCTCCGGGGTTACTCAATGTCTACGTGAAGGTCAATAAC
GGGCCACTGGGGAATCCTATCTGGAATATATCTGGAGACCCAGCACGTACATGGAACAGG
GCAGAACTGGCCATTAGTACTTTCTGGCCTAACTTTTATCAGGTGATTTTTGAAGTGATA
ACTTCTGGACATCAAGGCTATCTCGCTATCGATGAGGTGAAGGTGTTAGGACATCCATGT
ACCAGGACTCCTCACTTCCTGCGGATTCAGAATGTGGAAGTTAATGCTGGCCAGTTTGCT
ACCTTCCAGTGCAGTGCCATCGGCAGGACCGTGGCAGGAGACAGGCTCTGGTTACAGGGC
ATTGATGTGCGAGATGCTCCTCTGAAGGAAATCAAGGTGACCAGCTCCCGACGCTTCATT
GCTTCATTTAATGTTGTGAATACCACCAAACGAGATGCTGGAAAGTACCGCTGCATGATT
CGCACTGAAGGAGGTGTTGGAATATCAAACTATGCAGAGTTGGTAGTTAAAGAACCACCC
GTTCCTATTGCCCCACCTCAGCTCGCCTCTGTAGGAGCCACCTACCTGTGGATACAGCTC
AACGCCAACTCCATCAATGGGGATGGGCCCATTGTGGCCCGAGAGGTGGAGTACTGCACG
GCCAGTGGGAGCTGGAATGACCGGCAGCCAGTCGATTCCACGAGCTATAAAATTGGACAC
CTTGACCCAGATACAGAATATGAGATTAGTGTGCTCCTGACCAGGCCAGGGGAGGGTGGC
ACTGGCTCTCCTGGTCCGGCTCTCAGGACAAGAACAAAGTGTGCTGATCCCATGCGAGGC
CCAAGAAAACTAGAAGTAGTGGAGGTCAAATCTCGGCAAATCACTATCAGCTGGGAGCCA
TTTGGATATAATGTAACTCGTTGCCACAGTTATAATCTCACTGTCCACTACTGTTACCAA
GTTGGAGGACAAGAACAAGTGCGAGAAGAAGTAAGCTGGGATACAGAAAACTCACACCCT
CAACACACGATCACTAACCTGTCACCATACACCAATGTCAGTGTGAAACTGATCCTCATG
AACCCAGAGGGCCGGAAGGAAAGCCAAGAACTCATAGTGCAGACAGATGAAGACCTCCCA
GGTGCTGTTCCCACTGAATCCATACAAGGAAGTACCTTTGAAGAGAAGATATTTCTTCAG
TGGAGAGAACCAACTCAAACATATGGTGTAATCACTTTATATGAGATCACCTACAAAGCA
GTCAGTTCCTTTGACCCAGAAATAGATTTATCCAATCAGAGTGGAAGAGTTTCAAAGCTG
GGAAATGAAACCCATTTCCTGTTTTTTGGACTGTATCCGGGGACCACATACTCCTTTACC
ATCCGAGCTAGCACAGCTAAGGGTTTTGGGCCTCCAGCAACAAACCAGTTCACCACCAAA
ATATCAGCACCCTCTATGCCAGCTTATGAACTTGAGACACCTTTGAATCAAACTGACAAT
ACCGTGACAGTCATGCTGAAACCTGCCCACAGCAGAGGAGCACCTGTCAGTGTCTATCAA
ATAGTTGTTGAGGAAGAACGTCCTCGAAGAACTAAAAAGACGACAGAAATCTTAAAGTGC
TACCCAGTGCCAATTCACTTCCAGAATGCTTCTCTGCTGAACTCACAGTACTACTTTGCT
GCAGAATTTCCTGCAGACAGCCTCCAAGCTGCGCAGCCTTTTACAATTGGTGATAATAAG
ACATATAATGGATACTGGAACACTCCCCTTCTCCCCTATAAAAGCTACAGAATTTATTTC
CAAGCTGCTAGTAGAGCCAATGGGGAAACCAAAATAGACTGTGTCCAAGTGGCCACAAAA
GGAGCTGCCACTCCGAAACCAGTCCCAGAACCCGAGAAACAGACAGACCATACAGTTAAA
ATTGCTGGAGTCATCGCGGGCATCTTGCTGTTCGTGATTATATTTCTTGGAGTTGTGTTG
GTAATGAAGAAAAGGAAACTGGCCAAGAAGCGGAAAGAGACCATGAGCAGCACCCGACAG
GAGATGACTGTGATGGTGAACTCAATGGACAAGAGCTATGCTGAGCAGGGCACAAACTGC
GACGAGGCTTTCTCATTCATGGACACGCACAATCTGAATGGGAGATCTGTGTCTTCACCA
TCGTCCTTCACAATGAAAACAAATACACTGAGCACATCGGTGCCTAATTCCTATTACCCA
GATGAAACCCACACAATGGCCAGCGATACCAGCAGCCTGGTGCAGTCCCATACTTACAAG
AAGCGAGAGCCGGCCGACGTGCCCTATCAGACTGGGCAGCTCCACCCCGCCATCCGGGTG
GCAGACCTCCTTCAGCACATCACACAGATGAAGTGTGCGGAGGGCTACGGCTTCAAGGAG
GAATACGAGAGCTTCTTTGAAGGGCAGTCTGCACCATGGGACTCGGCTAAGAAAGGTGAG
AACAGAATGAAGAACAGATACGGGAATATCATTGCATACGATCATTCCCGAGTGAGGCTG
CAGACAATAGAAGGAGACACAAACTCAGACTATATCAATGGCAATTATATCGATGGTTAT
CATCGACCCAATCATTACATTGCTACCCAAGGGCCAATGCAGGAAACCATCTATGACTTC
TGGAGGATGGTGTGGCACGAAAACACTGCAAGTATCATCATGGTGACCAATCTTGTGGAA
GTGGGAAGGGTCAAATGCTGCAAATACTGGCCAGATGACACAGAGATATATAAAGACATT
AAAGTTACCCTAATAGAAACAGAACTACTGGCAGAATATGTGATAAGAACATTTGCTGTT
GAAAAGAGAGGTGTGCATGAAATCCGAGAGATCAGACAGTTTCACTTCACTGGCTGGCCG
GATCGTGGGGTCCCCTACCATGCCACCGGCCTGCTGGGATTCGTGCGGCAAGTCAAGTCC
AAGAGCCCGCCCAGTGCAGGCCCACTGGTGGTGCACTGCAGTGCTGGTGCAGGGAGGACT
GGCTGTTTCATCGTCATTGATATCATGTTGGACATGGCCGAAAGGGAAGGGGTCGTAGAC
ATCTACAACTGCGTCAGGGAGCTGCGGTCACGGAGGGTGAACATGGTGCAAACAGAGGAG
CAGTATGTGTTTATCCACGATGCGATCCTGGAAGCCTGTCTTTGTGGGGACACCTCTGTG
CCTGCTTCCCAAGTTAGGTCTCTGTATTATGACATGAACAAACTGGATCCACAGACAAAC
TCAAGCCAGATTAAAGAGGAATTCCGGACGCTAAACATGGTGACACCAACGCTGCGAGTA
GAGGACTGCAGCATCGCACTGTTGCCCCGGAACCATGAGAAAAACCGGTGCATGGACATC
CTGCCCCCAGACCGCTGCCTGCCCTTCCTCATCACCATCGATGGGGAGAGCAGCAACTAC
ATCAATGCTGCCCTCATGGACAGCTATAAACAGCCTTCAGCTTTTATAGTCACCCAGCAT
CCTTTGCCAAACACAGTGAAAGACTTTTGGAGACTGGTCCTGGATTATCACTGCACATCC
GTAGTTATGCTAAATGATGTGGATCCTGCCCAGTTGTGTCCACAGTACTGGCCAGAAAAC
GGAGTACACAGACACGGCCCCATCCAGGTGGAATTTGTCTCTGCTGACCTGGAAGAGGAC
ATCATCAGCAGGATATTCCGCATTTACAATGCCGCCAGACCCCAAGATGGATATCGGATG
GTGCAGCAATTCCAGTTCCTGGGCTGGCCGATGTACAGGGACACACCAGTGTCTAAGCGC
TCCTTCTTGAAGCTCATTCGCCAGGTGGACAAGTGGCAAGAGGAATACAATGGCGGGGAA
GGCCGCACCGTTGTGCACTGCTTGAACGGGGGAGGCCGCAGTGGGACGTTCTGCGCCATC
AGCATCGTATGTGAGATGCTCCGGCACCAGAGAACCGTGGATGTCTTTCACGCTGTGAAG
ACACTGAGGAACAACAAGCCCAACATGGTCGACCTCCTGGATCAGTACAAGTTCTGCTAC
GAGGTGGCCCTGGAATACTTGAATTCTGGC
Protein sequence predicted based on nucleotide sequence
MPSGSFMLVNASGRPEGQRAHLLLPQLKENDTHCIDFHYFVSSKSNSPPGLLNVYVKVNN GPLGNPIWNISGDPARTWNRAELAISTFWPNFYQVIFEVITSGHQGYLAIDEVKVLGHPC TRTPHFLRIQNVEVNAGQFATFQCSAIGRTVAGDRLWLQGIDVRDAPLKEIKVTSSRRFI ASFNVVNTTKRDAGKYRCMIRTEGGVGISNYAELVVKEPPVPIAPPQLASVGATYLWIQL NANSINGDGPIVAREVEYCTASGSWNDRQPVDSTSYKIGHLDPDTEYEISVLLTRPGEGG TGSPGPALRTRTKCADPMRGPRKLEVVEVKSRQITISWEPFGYNVTRCHSYNLTVHYCYQ VGGQEQVREEVSWDTENSHPQHTITNLSPYTNVSVKLILMNPEGRKESQELIVQTDEDLP GAVPTESIQGSTFEEKIFLQWREPTQTYGVITLYEITYKAVSSFDPEIDLSNQSGRVSKL GNETHFLFFGLYPGTTYSFTIRASTAKGFGPPATNQFTTKISAPSMPAYELETPLNQTDN TVTVMLKPAHSRGAPVSVYQIVVEEERPRRTKKTTEILKCYPVPIHFQNASLLNSQYYFA AEFPADSLQAAQPFTIGDNKTYNGYWNTPLLPYKSYRIYFQAASRANGETKIDCVQVATK GAATPKPVPEPEKQTDHTVKIAGVIAGILLFVIIFLGVVLVMKKRKLAKKRKETMSSTRQ EMTVMVNSMDKSYAEQGTNCDEAFSFMDTHNLNGRSVSSPSSFTMKTNTLSTSVPNSYYP DETHTMASDTSSLVQSHTYKKREPADVPYQTGQLHPAIRVADLLQHITQMKCAEGYGFKE EYESFFEGQSAPWDSAKKGENRMKNRYGNIIAYDHSRVRLQTIEGDTNSDYINGNYIDGY HRPNHYIATQGPMQETIYDFWRMVWHENTASIIMVTNLVEVGRVKCCKYWPDDTEIYKDI KVTLIETELLAEYVIRTFAVEKRGVHEIREIRQFHFTGWPDRGVPYHATGLLGFVRQVKS KSPPSAGPLVVHCSAGAGRTGCFIVIDIMLDMAEREGVVDIYNCVRELRSRRVNMVQTEE QYVFIHDAILEACLCGDTSVPASQVRSLYYDMNKLDPQTNSSQIKEEFRTLNMVTPTLRV EDCSIALLPRNHEKNRCMDILPPDRCLPFLITIDGESSNYINAALMDSYKQPSAFIVTQH PLPNTVKDFWRLVLDYHCTSVVMLNDVDPAQLCPQYWPENGVHRHGPIQVEFVSADLEED IISRIFRIYNAARPQDGYRMVQQFQFLGWPMYRDTPVSKRSFLKLIRQVDKWQEEYNGGE GRTVVHCLNGGGRSGTFCAISIVCEMLRHQRTVDVFHAVKTLRNNKPNMVDLLDQYKFCY EVALEYLNSG
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
