Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
CLOSED (STOP codon in vector)
Source:
None
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00741864
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 4113nts
Open reading frame: 1 to 4113
ATGACTACAAAACGAAGTTTGTTTGTGCGGTTGGTACCATGTCGCTGTCTACGAGGGGAA
GAGGAGACTGTCACTACTCTTGATTATTCTCATTGCAGCTTAGAACAAGTTCCGAAAGAG
ATTTTTACTTTTGAAAAAACCTTGGAGGAACTCTATTTAGATGCTAATCAGATTGAAGAG
CTTCCAAAGCAACTTTTTAACTGTCAGTCTTTACACAAACTGAGTTTGCCAGACAATGAT
TTAACAACGTTACCAGCATCCATTGCAAACCTTATTAATCTCAGGGAACTGGATGTCAGC
AAGAATGGAATACAGGAGTTTCCAGAAAATATAAAAAATTGTAAAGTTTTGACAATTGTG
GAGGCCAGTGTAAACCCTATTTCCAAGCTCCCTGATGGATTTTCTCAGCTGTTAAACCTA
ACCCAGTTGTATCTGAATGATGCTTTTCTTGAGTTCTTGCCAGCAAATTTTGGCAGATTA
ACTAAACTCCAAATATTAGAGCTTAGAGAAAACCAGTTAAAAATGTTGCCTAAAACTATG
AATAGACTGACCCAGCTGGAAAGATTGGATTTGGGAAGTAACGAATTCACGGAAGTGCCT
GAAGTACTTGAGCAACTAAGTGGATTGAAAGAGTTTTGGATGGATGCTAATAGACTGACT
TTTATTCCAGGGTTTATTGGTAGTTTGAAACAGCTCACATATTTGGATGTTTCTAAAAAT
AATATTGAAATGGTTGAAGAAGGAATTTCAACATGTGAAAACCTTCAAGACCTCCTATTA
TCAAGCAATTCACTTCAGCAGCTTCCTGAGCCTATTGGTTCGTTGAAGAATATAACAACG
CTTAAAATAGATGAAAACCAGTTAATGTATCTGCCAGACTCTATAGGAGGGTTAATATCA
GTAGAAGAACTGGATTGTAGTTTCAATGAAGTTGAAGCTTTGCCTTCATCTATTGGGCAG
CTTACTAACTTAAGAACTTTTGCTGCTGATCATAATTACTTACAACAGTTGCCCCCAGAG
ATTGGAAGCTGGAAAAATATAACTGTGCTGTTTCTCCATTCCAATAAACTTGAGACACTT
CCAGAGGAAATGGGTGATATGCAAAAATTAAAAGTCATTAATTTAAGTGATAATAGATTA
AAGAATTTACCCTTTAGCTTTACAAAGCTACAGCAATTGACAGCTATGTGGCTCTCAGAT
AATCAGTCCAAACCCCTGATACCTCTTCAAAAAGAAACTGATTCAGAGACCCAGAAAATG
GTGCTTACCAACTACATGTTCCCTCAACAGCCAAGGACTGAGGATGTTATGTTTATATCA
GATAATGAAAGTTTTAACCCTTCATTGTGGGAGGAACAGAGGAAACAGCGGGCTCAAGTT
GCATTTGAATGTGATGAAGACAAAGATGAAAGGGAGGCACCTCCCAGGGAGGGAAATTTA
AAAAGATATCCAACACCATACCCAGATGAGCTTAAGAATATGGTCAAAACTGTTCAAACC
ATTGTACATAGATTAAAAGATGAAGAGACCAATGAAGACTCAGGAAGAGATTTGAAACCA
CATGAAGATCAACAAGATATAAATAAAGATGTGGGTGTGAAGACCTCGGAAAGTACTACT
ACAGTAAAAAGCAAAGTTGGTGAAAGAGAAAAATATATGATAGGAAACTCTGTACAGAAG
ATCAGTGAACCTGAAGCTGAGATTAGTCCTGGGAGTTTACCAGTGACTGCAAATATGAAA
GCCTCTGAGAACTTGAAGCATATTGTTAACCATGATGATGTTTTTGAGGAATCTGAAGAA
CTTTCTTCTGATGAAGAGATGAAAATGGCGGAGATGCGACCACCATTAATTGAAACCTCT
ATTAACCAGCCAAAAGTCGTAGCACTTAGTAATAACAAAAAAGATGATACAAAGGAAACA
GATTCTTTATCAGATGAAGTTACACACAATAGCAATCAGAATAACAGCAATTGTTCTTCT
CCATCTCGGATGTCTGATTCAGTTTCTCTTAATACTGATAGTAGTCAAGACACCTCACTC
TGCTCTCCAGTGAAACAAACTCATATTGATATTAATTCCAAAATCAGGCAAGAAGATGAA
AATTTTAACAGCCTTTTACAAAATGGAGATATTTTAAACAGTTCAACAGAGGAAAAGTTC
AAAGCTCATGATAAAAAAGATTTTAACTTACCTGAATATGATTTGAATGTTGAAGAGCGA
TTAGTTCTAATTGAGAAAAGTGTTGACTCAACAGCCACAGCTGATGACACTCACAAATTA
GATCATATCAATATGAATCTTAATAAACTTATAACTAATGATACATTCCAACCAGAGATC
ATGGAAAGATCAAAAACACAGGATATTGTGCTTGGAACAAGCTTTTTAAGCATTAATTCT
AAAGAGGAAACTGAGCACTTGGAAAATGGAAACAAGTATCCTAATTTGGAATCCGTAAAT
AAGGTAAATGGACATTCTGAGGAAACTTCCCAGTCTCCTAATAGGACTGAACCACATGAC
AGTGATTGTTCTGTTGACTTAGGTATTTCCAAAAGCACTGAAGATCTCTCCCCTCAGAAA
AGTGGTCCAGTTGGATCTGTTGTGAAATCTCATAGCATAACTAATATGGAGATTGGAGGG
CTAAAAATCTATGATATTCTTAGTGATAATGGACCTCAGCAGCCAAGTACAACCGTTAAA
ATCACATCTGCTGTTGATGGAAAAAATATAGTCAGGAGCAAGTCTGCCACACTGTTGTAT
GATCAACCATTGCAGGTATTTACTGGTTCTTCCTCATCTTCTGATTTAATATCAGGAACA
AAGGCAATTTTCAAGTTTGATTCAAATCATAATCCCGAAGAGCCAAATATAATAAGAGGC
CCCACAAGTGGCCCACAATCTGCACCTCAAATATATGGTCCTCCACAGTATAATATCCAA
TACAGTAGCAGTGCTGCAGTCAAAGACACTTTGTGGCACTCCAAACAAAATCCCCAAATA
GACCATGCCAGTTTTCCTCCTCAGCTCCTTCCTAGATCAGAGAGCACAGAAAATCAAAGT
TATGCTAAACATTCTGCCAATATGAATTTCTCTAATCATAACAATGTTCGAGCTAATACT
GCATACCATTTACATCAGAGACTTGGCCCAGCAAGACATGGGGAAATGTGGGCCATCTCA
CCAAACGACCGACTTATTCCTGCAGTAACTCGAAGTACAATCCAGCGACAAAGTAGTGTG
TCCTCCACAGCCTCTGTAAATCTTGGTGATCCAGGCTCTACAAGGCGGGCTCAGATTCCT
GAAGGAGATTATTTATCATACAGAGAGTTCCACTCAGCGGGAAGAACTCCTCCAATGATG
CCAGGATCACAGAGACCCCTTTCTGCACGAACATACAGCATAGATGGTCCAAATGCATCA
AGACCTCAGAGTGCTCGACCCTCTATTAATGAAATACCAGAGAGAACTATGTCAGTTAGT
GATTTCAATTATTCACGGACTAGTCCTTCAAAAAGACCAAATGCAAGGGTTGGTTCTGAG
CATTCTTTATTAGATCCTCCAGGAAAAAGTAAAGTTCCTCGTGACTGGAGAGAACAAGTA
CTTCGACATATTGGAGCCAAAAAGTTAGAAAAGATGCCTTTGAGTAATGGACAGATGGGC
CAGCCTCTCAGGCCTCCGGCAAATTATAGTCAAATACATCACCCCCCTCAGGCATCTGTG
GCAAGGCATCCCTCTAGAGAACAACTAATTGATTACTTGATGCTGAAAGTGGCCCACCAG
CCTCCATATACACAGCCCCATTGTTCTCCTAGACAAGGCCATGAACTGGCAAAACAAGAG
ATTCGAGTGAGGGTTGAAAGGGATCCAGAACTTGGATTTAGCATATCAGGTGGTGTCGGG
GGTAGAGGAAACCCATTCAGACCTGATGATGATGGTATATTTGTAACAAGGGTACAACCT
GAAGGACCAGCATCAAAATTACTGCAGCCAGGTGATAAAATTATCCAGGCTAATGGCTAC
AGTTTTATAAATATTGAACATGGACAAGCAGTGTCCTTGCTAAAAACTTTCCAGAATACA
GTTGAACTCATCATTGTACGAGAAGTTTCCTCA
Protein sequence predicted based on nucleotide sequence
MTTKRSLFVRLVPCRCLRGEEETVTTLDYSHCSLEQVPKEIFTFEKTLEELYLDANQIEE LPKQLFNCQSLHKLSLPDNDLTTLPASIANLINLRELDVSKNGIQEFPENIKNCKVLTIV EASVNPISKLPDGFSQLLNLTQLYLNDAFLEFLPANFGRLTKLQILELRENQLKMLPKTM NRLTQLERLDLGSNEFTEVPEVLEQLSGLKEFWMDANRLTFIPGFIGSLKQLTYLDVSKN NIEMVEEGISTCENLQDLLLSSNSLQQLPEPIGSLKNITTLKIDENQLMYLPDSIGGLIS VEELDCSFNEVEALPSSIGQLTNLRTFAADHNYLQQLPPEIGSWKNITVLFLHSNKLETL PEEMGDMQKLKVINLSDNRLKNLPFSFTKLQQLTAMWLSDNQSKPLIPLQKETDSETQKM VLTNYMFPQQPRTEDVMFISDNESFNPSLWEEQRKQRAQVAFECDEDKDEREAPPREGNL KRYPTPYPDELKNMVKTVQTIVHRLKDEETNEDSGRDLKPHEDQQDINKDVGVKTSESTT TVKSKVGEREKYMIGNSVQKISEPEAEISPGSLPVTANMKASENLKHIVNHDDVFEESEE LSSDEEMKMAEMRPPLIETSINQPKVVALSNNKKDDTKETDSLSDEVTHNSNQNNSNCSS PSRMSDSVSLNTDSSQDTSLCSPVKQTHIDINSKIRQEDENFNSLLQNGDILNSSTEEKF KAHDKKDFNLPEYDLNVEERLVLIEKSVDSTATADDTHKLDHINMNLNKLITNDTFQPEI MERSKTQDIVLGTSFLSINSKEETEHLENGNKYPNLESVNKVNGHSEETSQSPNRTEPHD SDCSVDLGISKSTEDLSPQKSGPVGSVVKSHSITNMEIGGLKIYDILSDNGPQQPSTTVK ITSAVDGKNIVRSKSATLLYDQPLQVFTGSSSSSDLISGTKAIFKFDSNHNPEEPNIIRG PTSGPQSAPQIYGPPQYNIQYSSSAAVKDTLWHSKQNPQIDHASFPPQLLPRSESTENQS YAKHSANMNFSNHNNVRANTAYHLHQRLGPARHGEMWAISPNDRLIPAVTRSTIQRQSSV SSTASVNLGDPGSTRRAQIPEGDYLSYREFHSAGRTPPMMPGSQRPLSARTYSIDGPNAS RPQSARPSINEIPERTMSVSDFNYSRTSPSKRPNARVGSEHSLLDPPGKSKVPRDWREQV LRHIGAKKLEKMPLSNGQMGQPLRPPANYSQIHHPPQASVARHPSREQLIDYLMLKVAHQ PPYTQPHCSPRQGHELAKQEIRVRVERDPELGFSISGGVGGRGNPFRPDDDGIFVTRVQP EGPASKLLQPGDKIIQANGYSFINIEHGQAVSLLKTFQNTVELIIVREVSS
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
