Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
CLOSED (STOP codon in vector)
Source:
None
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00741881
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 3693nts
Open reading frame: 1 to 3693
ATGATAGCAGCTCCAGAAATACCAACTGATTTTAATCTACTACAGGAGTCAGAAACACAT
TTTTCTTCTGACACAGATTTTGAAGATATCGAAGGAAAAAACCAAAAGCAAGGCAAAGGC
AAAACTTGTAAAAAAGGCAAAAAGGGCCCAGCAGAAAAGGGCAAAGGTGGAAATGGAGGA
GGAAAACCTCCTTCTGGTCCAAACCGAATGAATGGTCATCACCAACAGAATGGAGTGGAA
AACATGATGTTGTTTGAAGTTGTTAAAATGGGCAAGAGTGCTATGCAGTCGGTGGTAGAT
GATTGGATAGAATCATACAAGCATGACCGAGATATAGCACTTCTTGACCTTATCAACTTT
TTTATTCAGTGTTCAGGCTGTAAAGGAGTTGTCACAGCAGAAATGTTTAGACATATGCAG
AACTCTGAGATAATTCGAAAAATGACTGAAGAATTCGATGAGGATAGTGGAGATTATCCA
CTTACCATGGCTGGTCCTCAGTGGAAGAAGTTCAAATCCAGTTTTTGTGAATTCATTGGC
GTGTTAGTACGGCAATGTCAATATAGTATCATATATGATGAGTATATGATGGATACAGTC
ATTTCACTTCTTACAGGATTGTCTGACTCACAAGTCAGAGCATTTCGACATACAAGCACC
CTGGCAGCTATGAAGTTGATGACAGCTTTGGTGAATGTGGCACTAAATCTTAGCATTAAT
ATGGATAATACACAAAGACAATATGAAGCAGAACGGAATAAAATGATTGGAAAACGAGCC
AATGAGAGGCTAGAACTCCTGCTACAAAAGCGGAAAGAGCTTCAGGAAAATCAAGATGAA
ATAGAAAATATGATGAATGCAATATTTAAAGGAGTGTTTGTACATAGATACCGTGATGCG
ATAGCTGAAATTCGAGCTATTTGCATTGAAGAGATTGGCATTTGGATGAAGATGTATAGT
GATGCCTTTCTTAATGACAGTTATTTAAAATATGTTGGTTGGACTATGCATGATAAGCAA
GGTGAAGTAAGACTCAAATGTCTTACTGCTCTACAAGGGCTTTATTATAACAAAGAGCTT
AATTCCAAACTGGAACTTTTTACCAGTCGGTTCAAGGATAGAATTGTGTCTATGACCCTT
GACAAAGAATATGATGTTGCAGTACAAGCAATAAAATTACTCACTCTTGTTTTACAGAGT
AGTGAAGAAGTTCTCACTGCAGAAGATTGTGAAAATGTCTATCATCTGGTTTATTCAGCT
CACCGGCCAGTAGCAGTAGCAGCTGGAGAATTTCTCTACAAAAAGCTCTTCAGTCGTAGA
GATCCAGAGGAGGATGGAATGATGAAAAGAAGAGGAAGACAAGGTCCAAATGCCAACCTT
GTTAAGACATTGGTTTTTTTCTTTCTAGAAAGTGAGTTACATGAGCATGCAGCATACCTT
GTGGATAGCATGTGGGACTGTGCTACTGAGCTGCTGAAAGACTGGGAATGTATGAATAGC
TTGTTACTGGAAGAGCCACTTAGTGGAGAGGAAGCACTAACAGATAGGCAAGAGAGTGCT
CTGATTGAAATAATGCTTTGTACCATTAGACAAGCGGCTGAATGTCATCCTCCCGTGGGA
AGAGGGACAGGAAAAAGGGTGCTTACAGCAAAGGAGAAGAAGACACAGTTGGATGATAGG
ACAAAAATCACTGAGCTTTTTGCCGTGGCCCTTCCTCAGTTATTAGCAAAATACTCTGTA
GATGCAGAAAAGGTGACTAACTTGTTGCAGTTGCCTCAGTACTTTGATTTGGAAATATAT
ACCACTGGACGATTAGAAAAGCATTTGGATGCCTTATTGCGACAGATCCGGAATATTGTA
GAGAAGCACACAGATACAGATGTTTTGGAAGCATGTTCTAAAACTTACCATGCACTCTGT
AATGAAGAGTTCACAATCTTCAACAGAGTAGATATTTCAAGAAGTCAACTGATAGATGAA
TTGGCAGATAAATTTAACCGGCTTCTTGAAGATTTTCTGCAAGAGGGTGAAGAACCTGAT
GAAGATGATGCATATCAGGTATTGTCAACATTGAAGAGGATCACTGCTTTTCATAATGCC
CATGACCTTTCAAAGTGGGATTTATTTGCTTGTAATTACAAACTCTTGAAAACTGGAATC
GAAAATGGAGACATGCCTGAGCAGATTGTTATTCACGCACTGCAGTGTACTCACTATGTA
ATCCTTTGGCAACTTGCTAAGATAACTGAAAGCAGCTCTACAAAGGAGGACTTGCTGCGT
TTAAAGAAACAAATGAGAGTATTTTGTCAGATATGTCAACATTACCTGACCAACGTGAAT
ACTACTGTTAAGGAACAGGCCTTCACTATTCTGTGTGATATTTTGATGATCTTCAGCCAT
CAGATTATGTCAGGAGGGCGTGACATGTTAGAGCCATTAGTGTATACCCCTGATTCTTCA
TTGCAGTCTGAGTTGCTCAGCTTTATTTTGGATCATGTCTTCATTGAACAGGATGATGAT
AATAATAGTGCAGATGGTCAGCAAGAGGATGAAGCCAGTAAAATTGAAGCTCTGCACAAG
AGAAGAAATTTACTTGCAGCATTTTGTAAGCTAATTGTATATACTGTGGTGGAGATGAAT
ACAGCTGCAGATATCTTCAAACAGTATATGAAGTATTATAATGACTATGGAGATATCATC
AAAGAAACAATGAGTAAAACAAGGCAGATAGACAAAATTCAGTGTGCTAAGACCCTTATT
CTCAGTCTGCAACAGCTTTTTAATGAAATGATACAAGAAAATGGCTATAATTTTGATAGA
TCATCCTCTACATTTAGTGGCATAAAAGAACTTGCTCGACGTTTTGCTTTAACTTTTGGA
CTTGATCAGTTGAAAACAAGAGAAGCCATTGCCATGCTACACAAAGATGGCATAGAATTT
GCTTTTAAAGAGCCTAATCCGCAAGGGGAGAGCCATCCACCTTTAAATTTGGCATTTCTT
GATATTCTGAGTGAATTTTCTTCTAAACTACTTCGACAAGACAAAAGAACAGTGTATGTT
TACTTGGAAAAGTTCATGACCTTTCAGATGTCACTCCGAAGAGAGGATGTGTGGCTTCCA
CTGATGTCTTACCGAAATTCTTTGCTAGCTGGTGGTGATGATGACACCATGTCAGTCATT
AGTGGAATCAGCAGCCGGGGGTCAACAGTACGGAGTAAAAAATCAAAACCATCTACAGGA
AAACGGAAAGTGGTTGAGGGCATGCAGCTTTCACTCACTGAAGAAAGTAGTAGTAGTGAC
AGTATGTGGTTAAGCAGAGAACAAACACTGCACACCCCTGTTATGATGCAGACACCACAA
CTCACCTCCACTATTATGAGAGAGCCCAAAAGATTACGGCCTGAGGATAGCTTCATGAGT
GTTTATCCAATGCAGACTGAACATCATCAAACACCTCTTGATTATAATCGGCGTGGCACA
AGCCTAATGGAAGATGATGAAGAGCCAATTGTGGAAGATGTTATGATGTCCTCAGAAGGG
AGGATTGAGGATCTTAATGAGGGAATGGATTTTGACACCATGGATATAGATTTGCCACCA
TCAAAGAACAGACGAGAGAGAACAGAACTGAAGCCTGATTTCTTTGATCCAGCTTCAATT
ATGGATGAATCAGTTCTTGGAGTGTCAATGTTT
Protein sequence predicted based on nucleotide sequence
MIAAPEIPTDFNLLQESETHFSSDTDFEDIEGKNQKQGKGKTCKKGKKGPAEKGKGGNGG GKPPSGPNRMNGHHQQNGVENMMLFEVVKMGKSAMQSVVDDWIESYKHDRDIALLDLINF FIQCSGCKGVVTAEMFRHMQNSEIIRKMTEEFDEDSGDYPLTMAGPQWKKFKSSFCEFIG VLVRQCQYSIIYDEYMMDTVISLLTGLSDSQVRAFRHTSTLAAMKLMTALVNVALNLSIN MDNTQRQYEAERNKMIGKRANERLELLLQKRKELQENQDEIENMMNAIFKGVFVHRYRDA IAEIRAICIEEIGIWMKMYSDAFLNDSYLKYVGWTMHDKQGEVRLKCLTALQGLYYNKEL NSKLELFTSRFKDRIVSMTLDKEYDVAVQAIKLLTLVLQSSEEVLTAEDCENVYHLVYSA HRPVAVAAGEFLYKKLFSRRDPEEDGMMKRRGRQGPNANLVKTLVFFFLESELHEHAAYL VDSMWDCATELLKDWECMNSLLLEEPLSGEEALTDRQESALIEIMLCTIRQAAECHPPVG RGTGKRVLTAKEKKTQLDDRTKITELFAVALPQLLAKYSVDAEKVTNLLQLPQYFDLEIY TTGRLEKHLDALLRQIRNIVEKHTDTDVLEACSKTYHALCNEEFTIFNRVDISRSQLIDE LADKFNRLLEDFLQEGEEPDEDDAYQVLSTLKRITAFHNAHDLSKWDLFACNYKLLKTGI ENGDMPEQIVIHALQCTHYVILWQLAKITESSSTKEDLLRLKKQMRVFCQICQHYLTNVN TTVKEQAFTILCDILMIFSHQIMSGGRDMLEPLVYTPDSSLQSELLSFILDHVFIEQDDD NNSADGQQEDEASKIEALHKRRNLLAAFCKLIVYTVVEMNTAADIFKQYMKYYNDYGDII KETMSKTRQIDKIQCAKTLILSLQQLFNEMIQENGYNFDRSSSTFSGIKELARRFALTFG LDQLKTREAIAMLHKDGIEFAFKEPNPQGESHPPLNLAFLDILSEFSSKLLRQDKRTVYV YLEKFMTFQMSLRREDVWLPLMSYRNSLLAGGDDDTMSVISGISSRGSTVRSKKSKPSTG KRKVVEGMQLSLTEESSSSDSMWLSREQTLHTPVMMQTPQLTSTIMREPKRLRPEDSFMS VYPMQTEHHQTPLDYNRRGTSLMEDDEEPIVEDVMMSSEGRIEDLNEGMDFDTMDIDLPP SKNRRERTELKPDFFDPASIMDESVLGVSMF
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
