Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
FUSION
Source:
None
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00741962
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 2985nts
Open reading frame: 1 to 2985
ATGGAGAAGACAGATGCCAAAGACCAGTCCTCTCAGGGGGATGAAGAGAAAGACCCTCCG
AAGAGCCACCCTTATTCTGTGGAGACCCCATATGGCTTTCATTTAGACCTGGACTTCCTC
AAGTATGTGGATGACATCGAGAAGGGAAACACTATCAAAAGAATTCCTATCCACAGAAGG
GCCAAGCAGGCCAAATTTAGCACTCTGCCCCGAAACTTCAGCCTTCCTGACAGTGGGGCT
CGCCCCCCTGCAGCCCCGCCCCTCCAAAACTGGTCTCCCGTGGTGCCAAGGGAGGCATCA
CTTGGGACACAGGAGCAAAACCAGTCGCCGCCGCTTGGTAATGCCCCCCAGGCCTCAACA
AGCAGGAGTGAGGTGAGCTACCACAGGAAGGCTCTGTTGGCAGAGGCCACCAGACAGTTG
GAAGCTGCTGAGCCAGAGGATGCCGAGCTCACTTTTGGGAGTGGACGGCCCCAGCTCTTG
AGAGCATCCAGCATGCCTGCCACGCTGCTGCACAGCAGGGCTTCTGAGGAGCCAGGCCTG
AGCCTGGGGCCCCCTGCCCCTCCTGCCCTCCCTCCCCTTCAGGGTGAAGGCAGTGTCTGT
GATGGCACCTTTGAACCTGCAGAAGGATTGGCAGGTTTCCACAGCTCCAGCCCACGAGCA
TCAACTCGGATTCCAGAGCTGGTCCAGGAGGGAGCTGAGCCTCCAGAGGGCGTGGTGAAG
GTTCCAAATCACCTCCCTCTCCCAGGCCCTCCTTTCTCATTCCAGAATGTGCTTGTAGTT
CTAGAGGACAAGGAAGATGAACACAATGCCAGAGAAGCAGAGGTGTTGTTCACCCCTGGC
TCCCCTACGCCAAGCCCGCCACCTCTGCCATCACCCATCCCTGAGAATGAGCTCCTCCTG
GAAGAAATCGAGCTCAACATCAGCGAGATTCCACCCCCGCCACCTGTAGAGGTGGACATG
AGAAGCATTGGCATCAGGGTAACTGAGGAAAGCCTGGGCCTTGCCAGGGTGGATCCAGGC
AGCATCTCCAGCCTGAAACAGCAGGTCTCGGCCCTGGAGGGAGAGTTGTCTGGAAGAACC
GAGGAACTGGCACAGGTCAGAACTGCTCTCCAGCAGCAGGAAGAGGAAATCAAAGCTAGG
GAGCAAAGAATTCGAGAGCTGGAGTTCACTGTAGCCCAACTGGAAGGACAGTTTCACCAA
GAGAACGCCAAAGACACTCAGGGCCAGACGGACGTGATGGTGAACACTGACCCTGTCCAT
GGACTCTTGACCAGGGAGTCGTGTGATAAGGGCATTGAAGTCAACCTTCTAGGCAGCATG
GAGTCTGAAAGCTGGGGGCACCGAGGAGAGGAGAATGGCCTCCTATGGGGGCCAGATGGT
CATAAACAAGGGAATCAGAGCCCAGCAGAACGTGTGCTTCTGCCCCAGCTGTCACTGCCA
CAGGGACCCGAGCAGGTCCTTACCTCCTCTGTACATAGCTTCCTCTCCACTGAACTCAGG
ATTGAAGAAGCAGGCACTGAACAGGAAGGAGGCCCTCAGGGAGGAACCAGGGGAGCAGGA
GGCTTTCTGTGGGGCAGTGACAGAAAGACTCCCCCAGCAGGGAGGGAGGAGACCAGTTCC
AATCTCCCAGGGAAGGAGCACCCGGGAAGGCCACCAAGCTCGCCAACGGATGCCACTATT
GGGCAGTATGTGAAGAAGATCCAGGAGCTCCTGCAGGAGCAGTGGAACTGCCTGGAGCAT
GGGTACCCGGAGCTGGCCAGCGCCATCAAGCAGCCAGCCTCCAAGCTCAGCAGCATCCAG
AGCCAGCTGCTGAGCTCCCTCAACCTGCTGCTGTCGGCCTACTCGGCCCAGGCTCACCCA
CCCAAGGAGCCGCCGGCCTCCTCCTCCTCCCCGCCAGTGGAGATCTCCCCATCGACCAGC
CTTAAATCCATAATGAAAAAGAAAGACTATGGCTTCCGTGCAGGAGGTAATGGGACCAAA
AAGAACCTTCAGTTTGTTGGGGTTAACGGTGGGTATGAGACCACCTCAAGTGAGGAGACC
AGCGGTGAGGACAGCACCCCAGAGGACTTGTCTGACAGCGAGGCAGAGAAGAAGTGTGAC
GGCCCAGATCACAAGCATGTCAAAGATGCCCATCTCACCTGCGAGGCTGGGCAGGGCATC
CCTGAGGGCACCTGCCATGCTGCCCAGGAAAGTGGGCCTGGGGAAGAAGTCCCCCACTCC
AAGGCCGAGAGATATAAACCCTCAGAAGAATTTCTTAATGCATGCCGGGCACTGAGCCAG
CATCTGCCAGAAACTGGGACCACCACAGACCAGCTCTTGAGGCAAAGCTTGAACACCATC
AGTCAAGAGTGGTTCCGCGTCTCCAGCCGGAAGTCGTCTAGCCCTGCCGTGGTGGCCTCC
TACCTCCACGAGGTCCAGCCTCACTCCCCACACTTCCTGAAGCTGCTTGTCAACTTGGCC
GATCGCAACGGGAACACGGCCCTTCACTACAGCGTGTCCCACTCCAACTTCTCCATCGCG
AAGCTGCTGCTGGAGACAGGCGTCTGCAATGTGGACCATCAGAACAAAGCTGGCTACACT
GCCGTAATGATCACTCCCTTGGCTTCCGCAGAGACCAATGAAGACATGGCTGTTGTCTGG
AAGCTCTTAAGAGAAGGAAATGTGAACATTCAAGCTACTCAGGGAGGCCAGACTGCGCTG
ATGCTGGGAGTCAGCCACGACAGGGAGGACATGGTTCAAGCGCTGCTTAGCTGCCAGGCA
GATGTCAATCTGCAGGACCACGATGGATCCTCGGCCCTCATGCTGGCCTGTCACCATGGC
AACGTGGACCTGGTGCGGCTGCTCCTGGCACACCCAGCCTGCGACAGCAGCCTGACTGAC
AAGGCTGGCCGCACAGCTTTGTCCATCGCTCTGAAGTCACCCACCCATATGGAAATTGCT
GGGCTTCTGAGAGCCCACGCGGAGCAGGGCAGGTCCCTGGGGCTG
Protein sequence predicted based on nucleotide sequence
MEKTDAKDQSSQGDEEKDPPKSHPYSVETPYGFHLDLDFLKYVDDIEKGNTIKRIPIHRR AKQAKFSTLPRNFSLPDSGARPPAAPPLQNWSPVVPREASLGTQEQNQSPPLGNAPQAST SRSEVSYHRKALLAEATRQLEAAEPEDAELTFGSGRPQLLRASSMPATLLHSRASEEPGL SLGPPAPPALPPLQGEGSVCDGTFEPAEGLAGFHSSSPRASTRIPELVQEGAEPPEGVVK VPNHLPLPGPPFSFQNVLVVLEDKEDEHNAREAEVLFTPGSPTPSPPPLPSPIPENELLL EEIELNISEIPPPPPVEVDMRSIGIRVTEESLGLARVDPGSISSLKQQVSALEGELSGRT EELAQVRTALQQQEEEIKAREQRIRELEFTVAQLEGQFHQENAKDTQGQTDVMVNTDPVH GLLTRESCDKGIEVNLLGSMESESWGHRGEENGLLWGPDGHKQGNQSPAERVLLPQLSLP QGPEQVLTSSVHSFLSTELRIEEAGTEQEGGPQGGTRGAGGFLWGSDRKTPPAGREETSS NLPGKEHPGRPPSSPTDATIGQYVKKIQELLQEQWNCLEHGYPELASAIKQPASKLSSIQ SQLLSSLNLLLSAYSAQAHPPKEPPASSSSPPVEISPSTSLKSIMKKKDYGFRAGGNGTK KNLQFVGVNGGYETTSSEETSGEDSTPEDLSDSEAEKKCDGPDHKHVKDAHLTCEAGQGI PEGTCHAAQESGPGEEVPHSKAERYKPSEEFLNACRALSQHLPETGTTTDQLLRQSLNTI SQEWFRVSSRKSSSPAVVASYLHEVQPHSPHFLKLLVNLADRNGNTALHYSVSHSNFSIA KLLLETGVCNVDHQNKAGYTAVMITPLASAETNEDMAVVWKLLREGNVNIQATQGGQTAL MLGVSHDREDMVQALLSCQADVNLQDHDGSSALMLACHHGNVDLVRLLLAHPACDSSLTD KAGRTALSIALKSPTHMEIAGLLRAHAEQGRSLGL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
