Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
FUSION
Source:
None
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00742409
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 3444nts
Open reading frame: 1 to 3444
ATGGATGACTACATGGAGACGCTGAAGGATGAAGAGGACGCCTTGTGGGAGAATGTGGAG
TGTAACCGGCACATGCTCAGCCGCTATATCAACCCTGCCAAGCTCACGCCCTACCTGCGT
CAGTGTAAGGTCATTGATGAGCAGGATGAAGATGAAGTGCTTAATGCCCCTATGCTGCCA
TCCAAGATCAACCGAGCAGGCCGGCTGTTGGACATTCTACATACCAAGGGGCAAAGGGGC
TATGTGGTCTTCTTGGAGAGCCTAGAATTTTATTACCCAGAACTGTACAAACTGGTGACT
GGGAAAGAGCCCACTCGGAGATTCTCCACCATTGTGGTGGAGGAAGGCCACGAGGGCCTC
ACGCACTTCCTGATGAACGAGGTCATCAAGCTGCAGCAGCAGATGAAGGCCAAGGACCTG
CAACGCTGCGAGCTGCTGGCCAGGTTGCGGCAGCTGGAGGATGAGAAGAAGCAGATGACG
CTGACGCGCGTGGAGCTGCTAACCTTCCAGGAGCGGTACTACAAGATGAAGGAAGAGCGG
GACAGCTACAATGACGAGCTGGTCAAGGTGAAGGACGACAACTACAACTTAGCCATGCGC
TACGCACAGCTCAGTGAGGAGAAGAACATGGCGGTCATGAGGAGCCGAGACCTCCAACTC
GAGATCGATCAGCTAAAGCACCGGTTGAATAAGATGGAGGAGGAATGTAAGCTGGAGAGA
AATCAGTCTCTAAAACTGAAGAATGACATTGAAAATCGGCCCAAGAAGGAGCAGGTTCTG
GAACTGGAGCGGGAGAATGAAATGCTGAAGACCAAAAACCAGGAGCTGCAGTCCATCATC
CAGGCCGGGAAGCGCAGCCTGCCAGACTCAGACAAGGCCATCCTGGACATCTTGGAACAC
GACCGCAAGGAGGCCCTGGAGGACAGGCAGGAGCTGGTCAACAGGATCTACAACCTGCAG
GAGGAGGCCCGCCAGGCAGAGGAGCTGCGAGACAAGTACCTGGAGGAGAAGGAGGACCTG
GAGCTCAAGTGCTCGACCCTGGGAAAGGACTGTGAAATGTACAAGCACCGCATGAACACG
GTCATGCTGCAGCTGGAGGAGGTGGAGCGGGAGCGGGACCAGGCCTTCCACTCCCGAGAT
GAAGCTCAGACACAGTACTCGCAGTGCTTAATCGAAAAGGACAAGTACAGGAAGCAGATC
CGCGAGCTGGAGGAGAAGAACGATGAGATGAGGATCGAGATGGTGCGGCGGGAGGCCTGC
ATCGTCAACCTGGAGAGCAAGCTGCGGCGCCTCTCCAAGGACAGCAACAACCTGGACCAG
AGTCTGCCCAGGAACCTGCCAGTAACCATCATCTCTCAGGACTTTGGGGATGCCAGCCCC
AGGACCAATGGTCAAGAAGCTGACGATTCTTCCACCTCGGAGGAGTCACCTGAAGACAGC
AAGTACTTCCTGCCCTACCATCCGCCCCAGCGCAGGATGAACCTGAAGGGCATCCAGCTG
CAGAGAGCCAAATCCCCCATCAGCCTGAAGCGAACATCAGATTTTCAAGCCAAGGGGCAC
GAGGAAGAAGGCACGGATGCCAGCCCTAGCTCCTGCGGATCTCTGCCCATCACCAACTCC
TTCACCAAGATGCAGCCCCCCCGGAGCCGCAGCAGCATCATGTCAATCACCGCCGAGCCC
CCGGGAAACGACTCCATCGTCAGACGCTACAAGGAGGACGCGCCCCATCGCAGCACAGTC
GAAGAAGACAATGACAGCGGCGGGTTTGACGCCTTAGATCTGGATGATGACAGTCACGAA
CGCTACTCCTTCGGACCCTCCTCCATCCACTCCTCCTCCTCCTCCCACCAATCCGAGGGC
CTGGATGCCTACGACCTGGAGCAGGTCAACCTCATGTTCAGGAAGTTCTCTCTGGAAAGA
CCCTTCCGGCCTTCGGTCACCTCTGTGGGGCACGTGCGGGGCCCAGGGCCCTCGGTGCAG
CACACGACGCTGAATGGCGACAGCCTCACCTCCCAGCTCACCCTGCTGGGGGGCAACGCG
CGAGGGAGCTTCGTGCACTCGGTCAAGCCTGGCTCTCTGGCCGAGAAAGCCGGCCTCCGT
GAGGGCCACCAGCTGCTGCTGCTAGAAGGCTGCATCCGAGGCGAGAGGCAGAGTGTCCCG
TTGGACACATGCACCAAAGAGGAAGCCCACTGGACCATCCAGAGGTGCAGCGGCCCCGTC
ACGCTGCACTACAAGGTCAACCACGAAGGGTACCGGAAGCTGGTGAAGGACATGGAGGAC
GGCCTGATCACATCGGGGGACTCGTTCTACATCCGGCTGAACCTGAACATCTCCAGCCAG
CTGGACGCCTGCACCATGTCCCTGAAGTGTGACGATGTTGTGCACGTCCGTGACACCATG
TACCAGGACAGGCACGAGTGGCTGTGCGCGCGGGTCGACCCTTTCACAGACCATGACCTG
GATATGGGCACCATACCCAGCTACAGCCGAGCCCAGCAGCTCCTCCTGGTGAAACTGCAG
CGCCTGATGCACCGAGGCAGCCGGGAGGAGGTAGACGGCACCCACCACACCCTGCGGGCA
CTCCGGAACACCCTGCAGCCAGAAGAAGCGCTTTCAACAAGCGACCCCCGGGTCAGCCCC
CGTCTCTCGCGAGCAAGCTTCCTTTTTGGCCAGCTCCTTCAGTTCGTCAGCAGGTCCGAG
AACAAGTATAAGCGGATGAACAGCAACGAGCGGGTCCGCATCATCTCGGGGAGTCCGCTA
GGGAGCCTGGCCCGGTCCTCGCTGGACGCCACCAAGCTCTTGACTGAGAAGCAGGAAGAG
CTGGACCCTGAGAGCGAGCTGGGCAAGAACCTCAGCCTCATCCCCTACAGCCTGGTACGC
GCCTTCTACTGCGAGCGCCGCCGGCCCGTGCTCTTCACACCCACCGTGCTGGCCAAGACG
CTGGTGCAGAGGCTGCTCAACTCGGGAGGTGCCATGGAGTTCACCATCTGCAAGTCAGAT
ATCGTCACAAGAGATGAGTTCCTCAGAAGGCAGAAGACGGAGACCATCATCTACTCCCGA
GAGAAGAACCCCAACGCGTTCGAATGCATCGCCCCTGCCAACATTGAAGCTGTGGCCGCC
AAGAACAAGCACTGCCTGCTGGAGGCTGGGATCGGCTGCACAAGAGACTTGATCAAGTCC
AACATCTACCCCATCGTGCTCTTCATCCGGGTGTGTGAGAAGAACATCAAGAGGTTCAGA
AAGCTGCTGCCCCGACCTGAGACGGAGGAGGAGTTCCTGCGCGTGTGCCGGCTGAAGGAG
AAGGAGCTGGAGGCCCTGCCGTGCCTGTACGCCACGGTGGAACCTGACATGTGGGGCAGC
GTAGAGGAGCTGCTCCGCGTTGTCAAGGACAAGATCGGCGAGGAGCAGCGCAAGACCATC
TGGGTGGACGAGGACCAGCTGTTG
Protein sequence predicted based on nucleotide sequence
MDDYMETLKDEEDALWENVECNRHMLSRYINPAKLTPYLRQCKVIDEQDEDEVLNAPMLP SKINRAGRLLDILHTKGQRGYVVFLESLEFYYPELYKLVTGKEPTRRFSTIVVEEGHEGL THFLMNEVIKLQQQMKAKDLQRCELLARLRQLEDEKKQMTLTRVELLTFQERYYKMKEER DSYNDELVKVKDDNYNLAMRYAQLSEEKNMAVMRSRDLQLEIDQLKHRLNKMEEECKLER NQSLKLKNDIENRPKKEQVLELERENEMLKTKNQELQSIIQAGKRSLPDSDKAILDILEH DRKEALEDRQELVNRIYNLQEEARQAEELRDKYLEEKEDLELKCSTLGKDCEMYKHRMNT VMLQLEEVERERDQAFHSRDEAQTQYSQCLIEKDKYRKQIRELEEKNDEMRIEMVRREAC IVNLESKLRRLSKDSNNLDQSLPRNLPVTIISQDFGDASPRTNGQEADDSSTSEESPEDS KYFLPYHPPQRRMNLKGIQLQRAKSPISLKRTSDFQAKGHEEEGTDASPSSCGSLPITNS FTKMQPPRSRSSIMSITAEPPGNDSIVRRYKEDAPHRSTVEEDNDSGGFDALDLDDDSHE RYSFGPSSIHSSSSSHQSEGLDAYDLEQVNLMFRKFSLERPFRPSVTSVGHVRGPGPSVQ HTTLNGDSLTSQLTLLGGNARGSFVHSVKPGSLAEKAGLREGHQLLLLEGCIRGERQSVP LDTCTKEEAHWTIQRCSGPVTLHYKVNHEGYRKLVKDMEDGLITSGDSFYIRLNLNISSQ LDACTMSLKCDDVVHVRDTMYQDRHEWLCARVDPFTDHDLDMGTIPSYSRAQQLLLVKLQ RLMHRGSREEVDGTHHTLRALRNTLQPEEALSTSDPRVSPRLSRASFLFGQLLQFVSRSE NKYKRMNSNERVRIISGSPLGSLARSSLDATKLLTEKQEELDPESELGKNLSLIPYSLVR AFYCERRRPVLFTPTVLAKTLVQRLLNSGGAMEFTICKSDIVTRDEFLRRQKTETIIYSR EKNPNAFECIAPANIEAVAAKNKHCLLEAGIGCTRDLIKSNIYPIVLFIRVCEKNIKRFR KLLPRPETEEEFLRVCRLKEKELEALPCLYATVEPDMWGSVEELLRVVKDKIGEEQRKTI WVDEDQLL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
