Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
FUSION
Source:
None
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00742512
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 3552nts
Open reading frame: 1 to 3552
ATGGTGCTGCTCTGGGAGCCTGCAGGAGCCTGGCTTGCTCTGGGCCTGGCCCTGGCCCTG
GGCCCCAGCGTGGCCGCAGCTGCCCCTCGGCAGGACTGCACGGGCGTGGAGTGCCCGCCG
CTGGAGAACTGCATTGAGGAGGCGCTGGAGCCGGGTGCCTGCTGTGCCACGTGTGTGCAG
CAGGGCTGCGCCTGCGAGGGCTACCAGTACTATGACTGCCTACAGGGTGGCTTCGTGCGC
GGCCGCGTGCCCGCCGGTCAGTCCTATTTTGTGGACTTCGGGAGCACTGAGTGCTCCTGC
CCACCAGGCGGCGGCAAGATCAGCTGCCAGTTCATGCTGTGCCCGGAGCTGCCGCCCAAC
TGCATCGAGGCTGTAGTGGTGGCTGACAGCTGCCCACAGTGCGGCCAGGTGGGCTGCGTC
CACGCGGGCCACAAGTACGCCGCTGGCCACACTGTTCACCTGCCGCCCTGCCGGGCCTGC
CACTGCCCTGACGCCGGTGGAGAGCTCATCTGCTACCAGCTCCCCGGTTGCCACGGGAAC
TTCTCAGATGCCGAGGAGGGTGACCCCGAGCGACACTACGAAGACCCCTACAGCTATGAC
CAGGAGGTGGCCGAGGTGGAAGCAGCAACAGCCCTGGGGGGTGAGGTCCAGGCGGGTGCA
GTCCAGGCAGGCGCAGGGGGCCCCCCAGCTGCTCTGGGAGGTGGGAGTCAGCCACTGTCC
ACCATCCAGGCACCCCCCTGGCCAGCTGTCCTCCCCAGGCCCACAGCGGCTGCTGCCCTG
GGTCCCCCAGCCCCAGTGCAGGCCAAAGCTAGGAGAGTGACCGAGGACAGTGAGGAGGAA
GAAGAGGAGGAGGAGGAGAGAGAGGAAATGGCTGTCACTGAGCAGCTGGCAGCAGGTGGC
CACAGGGGGCTGGATGGGCTGCCCACTACAGCCCCAGCTGGACCCAGTCTTCCTATCCAG
GAGGAGAGGGCAGAAGCTGGGGCAAGGGCAGAAGCTGGGGCAAGGCCTGAAGAGAACCTC
ATCCTGGATGCCCAAGCCACGTCCCGCAGCACTGGGCCGGAGGGCGTGACGCATGCACCG
AGCCTGGGCAAGGCTGCTCTCGTCCCAACTCAGGCCGTGCCTGGCTCTCCCAGGGACCCA
GTCAAGCCCAGCCCCCACAACATCCTGTCCACATCACTGCCTGATGCAGCCTGGATCCCA
CCCACCCGAGAAGTGCCCAGGAAGCCGCAAGTTCTGCCCCATTCCCACGTGGAGGAGGAC
ACAGACCCCAACTCTGTCCATTCTATCCCCAGAAGTAGCCCTGAAGGCTCCACCAAGGAC
CTGATCGAGACTTGCTGCGCAGCCGGACAGCAGTGGGCCATTGACAATGACGAGTGCCTG
GAGATCCCTGAGAGTGGCACTGAGGACAACGTCTGCAGGACAGCCCAGAGGCACTGCTGT
GTCTCCTACTTGCAGGAGAAGAGCTGCATGGCCGGCGTCCTGGGAGCCAAGGAGGGTGAG
ACCTGTGGGGCTGAGGACAACGACAGCTGCGGCATCTCCCTGTACAAGCAATGCTGTGAC
TGCTGTGGCCTGGGCCTCCGCGTGCGGGCCGAGGGCCAGTCGTGTGAGTCCAATCCTAAC
CTGGGCTATCCCTGCAATCATGTCATGCTCTCCTGCTGTGAGGGTGAAGAGCCTCTCATA
GTACCTGAGGTTCGCCGACCTCCAGAGCCCGCAGCTGCACCACGGAGAGTTTCAGAGGCA
GAGATGGCGGGCCGAGAGGCCCTGTCACTGGGCACAGAGGCCGAGCTGCCGAACAGCCTG
CCGGGCGATGACCAGGATGAGTGCCTTCTCCTCCCGGGAGAGCTGTGCCAGCACCTTTGC
ATCAATACTGTGGGTTCTTACCACTGTGCCTGCTTTCCTGGCTTCTCACTGCAGGACGAT
GGCCGCACTTGCCGCCCAGAGGGTCACCCTCCACAGCCGGAAGCCCCACAGGAGCCTGCA
CTGAAGTCAGAATTTTCCCAGGTGGCCTCTAACACCATCCCGCTGCCACTGCCGCAGCCC
AATACCTGCAAAGACAATGGACCCTGCAAGCAGGTGTGCAGCACTGTTGGGGGCTCAGCC
ATATGCTCCTGTTTTCCCGGCTATGCCATCATGGCGGATGGCGTGTCCTGTGAAGACATC
AACGAGTGTGTGACGGACCTGCACACGTGCAGCCGGGGCGAGCACTGTGTGAACACACTG
GGCTCCTTCCACTGCTACAAGGCACTCACCTGTGAGCCAGGCTATGCCCTCAAGGATGGC
GAGTGCGAAGACGTGGATGAGTGTGCGATGGGCACGCACACCTGCCAGCCGGGCTTCTTG
TGCCAGAACACCAAGGGCTCCTTCTACTGCCAGGCCAGGCAGCGCTGCATGGATGGCTTC
CTGCAGGATCCTGAAGGCAACTGTGTGGACATCAACGAGTGCACGTCACTGTCCGAGCCA
TGTCGGCCAGGCTTCAGCTGCATCAACACGGTGGGCTCCTACACGTGCCAGAGGAACCCG
CTGATCTGCGCGCGCGGCTACCACGCCAGCGATGATGGGGCCAAGTGTGTGGACGTGAAT
GAGTGTGAGACAGGTGTGCACCGCTGCGGTGAGGGCCAAGTGTGCCACAACCTCCCTGGC
TCCTACCGCTGTGACTGCAAAGCCGGCTTTCAGCGGGATGCCTTCGGCCGGGGCTGCATC
GACGTGAATGAGTGCTGGGCCTCGCCAGGCCGCCTGTGCCAGCACACGTGTGAGAACACA
CTCGGCTCCTACCGCTGTTCCTGCGCCTCCGGGTTCCTGCTAGCAGCGGACGGCAAGCGC
TGTGAAGACGTGAATGAGTGTGAGGCCCAGCGCTGCAGCCAGGAGTGTGCCAACATCTAT
GGCTCCTACCAGTGCTACTGCCGCCAGGGCTACCAGCTGGCTGAGGATGGGCACACCTGC
ACAGACATCGACGAGTGTGCTCAAGGCGCCGGCATCCTCTGCACCTTCCGCTGTCTCAAC
GTGCCAGGGAGCTACCAGTGTGCATGCCCTGAGCAGGGCTACACCATGACGGCCAACGGG
AGGTCCTGCAAGGACGTGGATGAGTGTGCACTGGGTACCCACAACTGTTCCGAGGCTGAG
ACCTGCCACAACATCCAGGGTAGCTTCCGCTGCCTGCGCTTAGAGTGTCCTCCCAACTAT
GTCCAAGTCTCCAAAACGAAGTGCGAGCGCACCACGTGCCATGACTTCCTGGAGTGCCAG
AACTCGCCAGCGCGCATCACGCACTACCAGCTCAACTTCCAGACGGGCCTCCTGGTGCCT
GCGCATATCTTCCGCATTGGCCCCGCGCCAGCCTTCACGGGGGACACCATCGCCCTGAAC
ATCATCAAGGGCAATGAGGAGGGCTACTTTGGCACGCGCAGGCTCAATGCCTACACGGGT
GTGGTCTACCTGCAGCGGGCCGTGCTGGAGCCCCGGGACTTTGCCCTGGACGTGGAGATG
AAGCTCTGGAGGCAGGGCTCCGTCACCACCTTCCTGGCCAAGATGCACATCTTCTTCACC
ACCTTTGCCCTG
Protein sequence predicted based on nucleotide sequence
MVLLWEPAGAWLALGLALALGPSVAAAAPRQDCTGVECPPLENCIEEALEPGACCATCVQ QGCACEGYQYYDCLQGGFVRGRVPAGQSYFVDFGSTECSCPPGGGKISCQFMLCPELPPN CIEAVVVADSCPQCGQVGCVHAGHKYAAGHTVHLPPCRACHCPDAGGELICYQLPGCHGN FSDAEEGDPERHYEDPYSYDQEVAEVEAATALGGEVQAGAVQAGAGGPPAALGGGSQPLS TIQAPPWPAVLPRPTAAAALGPPAPVQAKARRVTEDSEEEEEEEEEREEMAVTEQLAAGG HRGLDGLPTTAPAGPSLPIQEERAEAGARAEAGARPEENLILDAQATSRSTGPEGVTHAP SLGKAALVPTQAVPGSPRDPVKPSPHNILSTSLPDAAWIPPTREVPRKPQVLPHSHVEED TDPNSVHSIPRSSPEGSTKDLIETCCAAGQQWAIDNDECLEIPESGTEDNVCRTAQRHCC VSYLQEKSCMAGVLGAKEGETCGAEDNDSCGISLYKQCCDCCGLGLRVRAEGQSCESNPN LGYPCNHVMLSCCEGEEPLIVPEVRRPPEPAAAPRRVSEAEMAGREALSLGTEAELPNSL PGDDQDECLLLPGELCQHLCINTVGSYHCACFPGFSLQDDGRTCRPEGHPPQPEAPQEPA LKSEFSQVASNTIPLPLPQPNTCKDNGPCKQVCSTVGGSAICSCFPGYAIMADGVSCEDI NECVTDLHTCSRGEHCVNTLGSFHCYKALTCEPGYALKDGECEDVDECAMGTHTCQPGFL CQNTKGSFYCQARQRCMDGFLQDPEGNCVDINECTSLSEPCRPGFSCINTVGSYTCQRNP LICARGYHASDDGAKCVDVNECETGVHRCGEGQVCHNLPGSYRCDCKAGFQRDAFGRGCI DVNECWASPGRLCQHTCENTLGSYRCSCASGFLLAADGKRCEDVNECEAQRCSQECANIY GSYQCYCRQGYQLAEDGHTCTDIDECAQGAGILCTFRCLNVPGSYQCACPEQGYTMTANG RSCKDVDECALGTHNCSEAETCHNIQGSFRCLRLECPPNYVQVSKTKCERTTCHDFLECQ NSPARITHYQLNFQTGLLVPAHIFRIGPAPAFTGDTIALNIIKGNEEGYFGTRRLNAYTG VVYLQRAVLEPRDFALDVEMKLWRQGSVTTFLAKMHIFFTTFAL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
