Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
Codon optimized
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
None
Description:
None
Comments:
Gene synthesis by Gen9. Codon optimized
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00744389
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 5214nts
Open reading frame: 1 to 5214
ATGCTCAAACGGAAGCAGAGTTCCAGGGTGGAAGCCCAGCCAGTCACTGACTTTGGTCCC
GATGAGTCCCTCTCGGATAATGCTGACATCCTCTGGATTAACAAACCATGGGTTCACTCT
TTGCTGCGCATCTGTGCCATCATCAGCGTCATTTCTGTTTGTATGAATACCCCCATGACC
TTTGAGCACTATCCTCCACTTCAGTATGTGACCTTCACTTTGGATACATTATTGATGTTT
CTCTACACGGCAGAGATGATAGCAAAAATGCACATCCGAGGCATTGTCAAGGGGGATAGT
TCCTATGTGAAAGATCGCTGGTGTGTTTTTGATGGATTTATGGTCTTTTGCCTTTGGGTT
TCTTTGGTGCTACAGGTGTTTGAAATTGCCGATATAGTTGATCAGATGTCACCCTGGGGC
ATGCTGCGGATTCCACGGCCACTGATTATGATCCGAGCATTCCGTATTTATTTCCGATTT
GAACTGCCAAGGACCAGGATTACAAATATTTTAAAGCGATCGGGAGAACAAATATGGTCT
GTTTCCATCTTTCTACTTTTCTTTCTACTTCTTTATGGAATTTTAGGAGTCCAGATGTTT
GGCACATTTACTTATCACTGTGTTGTAAATGACACAAAGCCCGGGAATGTGACCTGGAAT
AGTTTAGCTATTCCCGACACACACTGCTCACCAGAGCTAGAAGAAGGCTACCAGTGCCCA
CCTGGTTTTAAATGCATGGACCTTGAAGATCTGGGACTTAGCAGGCAGGAGCTGGGCTAC
AGCGGCTTTAATGAGATAGGAACTAGTATATTCACCGTCTATGAGGCCGCCTCACAGGAA
GGCTGGGTGTTCCTTATGTACAGAGCAATTGACAGCTTTCCCCGTTGGCGATCCTACTTC
TATTTCATCACCCTCATTTTCTTCCTGGCCTGGCTTGTGAAGAACGTGTTTATTGCTGTT
ATCATTGAAACATTTGCAGAAATCAGAGTGCAGTTTCAACAAATGTGGGGATCGAGAAGC
AGCACTACCTCAACAGCCACCACCCAGATGTTTCATGAAGATGCTGCCGGAGGTTGGCAG
CTCGTAGCTGTGGATGTCAACAAGCCCCAGGGACGCGCCCCAGCCTGTCTCCAGAAAATG
ATGCGGTCATCCGTTTTCCACATGTTCATCCTGAGTATGGTGACCGTGGACGTGATCGTG
GCGGCTAGTAACTACTACAAAGGAGAAAACTTCAGGAGGCAGTACGACGAGTTCTACCTG
GCGGAGGTGGCTTTTACAGTACTTTTTGATTTGGAAGCACTTCTCAAGATATGGTGTTTG
GGATTTACTGGATATATTAGCTCCTCTCTCCACAAATTCGAACTACTACTCGTAATTGGA
ACTACTCTTCATGTATACCCAGATCTGTATCATTCACAATTCACGTACTTTCAGGTTCTC
CGAGTAGTTCGGCTGATTAAGATTTCGCCTGCATTAGAAGACTTTGTGTACAAGATATTT
GGTCCTGGAAAAAAGCTCGGGAGTTTGGTCGTATTTACTGCCAGTCTCTTGATTGTTATG
TCAGCAATTAGTTTACAGATGTTCTGCTTTGTCGAAGAACTTGACAGATTTACTACGTTT
CCGCGGGCATTTATGTCCATGTTCCAGATCCTTACCCAGGAAGGATGGGTGGACGTAATG
GACCAAACTCTTAATGCTGTGGGACATATGTGGGCACCCGTGGTTGCCATCTATTTCATT
CTCTATCATCTTTTTGCCACTCTGATCCTTCTGAGTTTGTTTGTTGCTGTTATTTTGGAC
AACTTAGAACTTGATGAAGACCTAAAGAAGCTTAAACAATTAAAGCAAAGTGAAGCAAAT
GCGGACACCAAAGAAAAGCTCCCTTTACGCCTGCGAATCTTTGAAAAATTTCCAAACAGA
CCTCAAATGGTGAAAATCTCAAAGCTTCCTTCAGATTTTACAGTGCCTAAAATCAGGGAG
AGTTTTATGAAGCAGTTCATTGACCGCCAGCAACAGGACACCTGTTGCCTCCTGCGAAGC
CTCCCGACCACCTCTTCCTCCAGCTGCGACCACTCCAAACGCTCAGCAATTGAGGACAAC
AAATACATCGACCAAAAACTACGCAAGTCTGTTTTCAGCATCAGGGCAAGAAACCTTCTG
GAAAAGGAGACTGCAGTCACTAAAATCTTAAGAGCTTGCACCAGACAGCGCATGCTGAGC
GGATCATTTGAGGGGCAGCCCGCAAAGGAGAGGTCAATCCTCAGCGTGCAGCATCATATC
CGCCAAGAGCGCAGGTCACTGAGACATGGATCAAACAGCCAGAGGATCAGCCGGGGAAAA
TCGCTCGAAACTTTGACTCAAGATCATTCCAATACAGTGAGATATAGAAATGCACAAAGA
GAAGACAGTGAAATAAAGATGATTCAGGAAAAAAAGGAGCAAGCAGAGATGAAAAGGAAA
GTGCAAGAAGAGGAACTCAGAGAGAACCACCCATACTTCGATAAGCCACTGTTCATTGTC
GGGCGAGAGCACAGGTTCAGGAACTTTTGCCGGGTCGTGGTCCGAGCACGCTTTAACGCA
TCTAAAACAGACCCTGTCACAGGAGCTGTGAAAAATACAAAGTACCATCAACTTTACGAT
TTGCTGGGATTGGTCACTTACCTGGACTGGGTCATGATCATCGTAACCATCTGCTCTTGC
ATTTCGATGATGTTTGAGTCCCCGTTCCGAAGAGTCATGCATGCACCTACTTTGCAGATT
GCTGAGTATGTCTTTGTGATTTTCATGAGCATTGAGCTCAATCTGAAGATCATGGCAGAT
GGCTTATTTTTCACTCCAACTGCTGTCATTAGGGACTTCGGTGGAGTAATGGACATATTT
ATATATCTTGTGAGCTTGATATTTCTTTGTTGGATGCCTCAAAATGTACCTGCTGAATCG
GGAGCTCAGCTTCTAATGGTCCTTCGCTGCCTGCGTCCTCTGCGCATATTCAAACTGGTG
CCCCAGATGAGGAAAGTGGTTCGAGAACTGTTCAGCGGCTTCAAGGAAATTTTTTTGGTG
TCCATTCTTTTGCTGACATTAATGCTCGTTTTTGCAAGCTTTGGAGTGCAGCTTTTTGCT
GGAAAACTGGCCAAGTGCAATGATCCCAACATTATTAGAAGGGAAGATTGCAATGGCATA
TTCAGAATTAATGTCAGTGTGTCAAAGAACTTAAATCTCAAATTGAGGCCTGGAGAGAAA
AAACCCGGATTTTGGGTGCCCCGTGTTTGGGCCAATCCTCGGAACTTTAATTTCGACAAT
GTCGGAAACGCTATGCTGGCCCTGTTTGAAGTTCTCTCCTTGAAAGGCTGGGTGGAAGTG
AGAGATGTTATTATTCATCGTGTCGGGCCGATCCATGGAATCTATATTCATGTTTTTGTA
TTCCTGGGTTGCATGATTGGACTGACCCTTTTTGTTGGAGTAGTTATTGCTAATTTCAAT
GAAAACAAGGGGACGGCTTTGCTGACCGTCGATCAGAGAAGATGGGAAGACCTGAAGAGC
CGACTGAAGATCGCACAGCCTCTTCATCTTCCGCCTCGCCCGGATAATGATGGTTTTAGA
GCTAAAATGTATGACATAACCCAGCATCCATTTTTTAAGAGGACAATCGCATTACTCGTC
CTCGCCCAGTCGGTGTTGCTCTCTGTCAAGTGGGACGTCGAGGACCCGGTGACCGTACCT
CTGGCAACAATGTCAGTTGTTTTCACCTTCATCTTTGTTCTGGAGGTTACCATGAAGATC
ATAGCAATGTCGCCTGCTGGCTTCTGGCAAAGCAGAAGAAACCGGTACGATCTCCTGGTG
ACGTCGCTTGGCGTGGTATGGGTGGTGCTTCACTTTGCCCTCCTCAACGCCTATACTTAC
ATGATGGGCGCATGTGTGATTGTATTTAGGTTTTTCTCCATCTGTGGAAAACATGTAACG
CTCAAGATGCTCCTCTTGACAGTGGTCGTCAGCATGTACAAGAGCTTCTTTATCATAGTA
GGCATGTTTCTCTTGCTGCTGTGTTACGCTTTTGCTGGAGTTGTTTTATTTGGTACTGTG
AAATATGGGGAGAATATTAACAGGCATGCCAATTTTTCTTCCGCTGGAAAAGCCATTACC
GTACTGTTCCGAATTGTCACAGGTGAAGACTGGAACAAGATTATGCATGACTGTATGGTT
CAGCCTCCGTTTTGTACTCCAGATGAATTTACATACTGGGCAACAGACTGTGGAAATTAT
GCTGGGGCACTTATGTATTTCTGCTCATTTTATGTCATCATTGCCTACATCATGCTAAAT
CTGCTTGTAGCCATAATTGTGGAGAATTTCAGCTTGTTTTATTCCACTGAGGAGGACCAG
CTTTTAAGTTACAATGATCTTCGCCACTTTCAAATAATATGGAACATGGTGGATGATAAA
CGAGAGGGGGTGATCCCCACGTTCCGCGTCAAGTTCCTGCTGCGGCTACTGCGTGGGAGG
CTCGAGGTGGACCTGGACAAGGACAAGCTCCTGTTTAAGCACATGTGCTACGAAATGGAG
AGGCTCCACAATGGCGGCGACGTCACCTTCCATGATGTCCTGAGCATGCTCTCATACCGG
TCCGTGGACATCCGGAAGAGCTTGCAGCTGGAGGAACTCCTGGCGAGGGAGCAGCTGGAG
TACACCATAGAGGAGGAGGTGGCCAAGCAGACCATCCGCATGTGGCTCAAGAAGTGCCTG
AAGCGCATCAGAGCTAAACAGCAGCAGTCGTGCAGTATCATCCACAGCCTGAGAGAGAGT
CAGCAGCAAGAGCTGAGTCGGTTTCTGAACCCGCCCAGCATCGAGACTACCCAGCCCAGT
GAGGACACGAATGCCAACAGTCAGGACAACAGCATGCAACCTGAGACAAGCAGCCAGCAG
CAGCTCCTGAGCCCCACGTTGTCTGATCGAGGAGGCAGTCGGCAAGATGCAGCTGACGCA
GGCAAACCCCAGAGGAAATTTGGGCAGTGGCGTCTGCCCTCAGCCCCCAAACCAATAAGC
CATTCCGTGTCCTCCGTCAACTTAAGGTTTGGAGGAAGGACAACCATGAAATCTGTCGTG
TGCAAAATGAACCCCATGACTGACGCGGCTTCCTGCGGTTCTGAAGTTAAGAAGTGGTGG
ACCCGGCAGCTGACTGTGGAGAGCGACGAAAGTGGGGATGACCTTCTGGATATT
Protein sequence predicted based on nucleotide sequence
MLKRKQSSRVEAQPVTDFGPDESLSDNADILWINKPWVHSLLRICAIISVISVCMNTPMT FEHYPPLQYVTFTLDTLLMFLYTAEMIAKMHIRGIVKGDSSYVKDRWCVFDGFMVFCLWV SLVLQVFEIADIVDQMSPWGMLRIPRPLIMIRAFRIYFRFELPRTRITNILKRSGEQIWS VSIFLLFFLLLYGILGVQMFGTFTYHCVVNDTKPGNVTWNSLAIPDTHCSPELEEGYQCP PGFKCMDLEDLGLSRQELGYSGFNEIGTSIFTVYEAASQEGWVFLMYRAIDSFPRWRSYF YFITLIFFLAWLVKNVFIAVIIETFAEIRVQFQQMWGSRSSTTSTATTQMFHEDAAGGWQ LVAVDVNKPQGRAPACLQKMMRSSVFHMFILSMVTVDVIVAASNYYKGENFRRQYDEFYL AEVAFTVLFDLEALLKIWCLGFTGYISSSLHKFELLLVIGTTLHVYPDLYHSQFTYFQVL RVVRLIKISPALEDFVYKIFGPGKKLGSLVVFTASLLIVMSAISLQMFCFVEELDRFTTF PRAFMSMFQILTQEGWVDVMDQTLNAVGHMWAPVVAIYFILYHLFATLILLSLFVAVILD NLELDEDLKKLKQLKQSEANADTKEKLPLRLRIFEKFPNRPQMVKISKLPSDFTVPKIRE SFMKQFIDRQQQDTCCLLRSLPTTSSSSCDHSKRSAIEDNKYIDQKLRKSVFSIRARNLL EKETAVTKILRACTRQRMLSGSFEGQPAKERSILSVQHHIRQERRSLRHGSNSQRISRGK SLETLTQDHSNTVRYRNAQREDSEIKMIQEKKEQAEMKRKVQEEELRENHPYFDKPLFIV GREHRFRNFCRVVVRARFNASKTDPVTGAVKNTKYHQLYDLLGLVTYLDWVMIIVTICSC ISMMFESPFRRVMHAPTLQIAEYVFVIFMSIELNLKIMADGLFFTPTAVIRDFGGVMDIF IYLVSLIFLCWMPQNVPAESGAQLLMVLRCLRPLRIFKLVPQMRKVVRELFSGFKEIFLV SILLLTLMLVFASFGVQLFAGKLAKCNDPNIIRREDCNGIFRINVSVSKNLNLKLRPGEK KPGFWVPRVWANPRNFNFDNVGNAMLALFEVLSLKGWVEVRDVIIHRVGPIHGIYIHVFV FLGCMIGLTLFVGVVIANFNENKGTALLTVDQRRWEDLKSRLKIAQPLHLPPRPDNDGFR AKMYDITQHPFFKRTIALLVLAQSVLLSVKWDVEDPVTVPLATMSVVFTFIFVLEVTMKI IAMSPAGFWQSRRNRYDLLVTSLGVVWVVLHFALLNAYTYMMGACVIVFRFFSICGKHVT LKMLLLTVVVSMYKSFFIIVGMFLLLLCYAFAGVVLFGTVKYGENINRHANFSSAGKAIT VLFRIVTGEDWNKIMHDCMVQPPFCTPDEFTYWATDCGNYAGALMYFCSFYVIIAYIMLN LLVAIIVENFSLFYSTEEDQLLSYNDLRHFQIIWNMVDDKREGVIPTFRVKFLLRLLRGR LEVDLDKDKLLFKHMCYEMERLHNGGDVTFHDVLSMLSYRSVDIRKSLQLEELLAREQLE YTIEEEVAKQTIRMWLKKCLKRIRAKQQQSCSIIHSLRESQQQELSRFLNPPSIETTQPS EDTNANSQDNSMQPETSSQQQLLSPTLSDRGGSRQDAADAGKPQRKFGQWRLPSAPKPIS HSVSSVNLRFGGRTTMKSVVCKMNPMTDAASCGSEVKKWWTRQLTVESDESGDDLLDI
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
