Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
Codon optimized
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
None
Description:
None
Comments:
Gene synthesis by Gen9. Codon optimized
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00744420
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 5886nts
Open reading frame: 1 to 5886
ATGAGAGAATTGGAAGCTAAAGCTACCAAAGACGTAGAAAGAAATCTGAGCAGGGATTTA
GTGCAGGAAGAAGAGCAGTTAATGGAAGAAAAGAAGAAGAAAAAAGACGACAAAAAAAAG
AAAGAAGCTGCTCAAAAGAAGGCCACTGAACAAAAAATCAAAGTGCCAGAACAGATAAAG
CCCAGTGTAAGCCAGCCTCAGCCTGCCAATTCTAATAACGGCACCTCCACAGCAACTAGC
ACCAATAATAATGCCAAGCGAGCTACAGCCAACAATCAGCAACCCCAGCAGCAGCAGCAA
CAACAGCAACCTCAGCAGCAGCAGCCGCAGCAGCAGCCTCAACCGCAGCCGCAACAGCAG
CAGCCACAGCAGCAACCACAGGCCTTGCCTCGGTATCCTCGTGAAGTCCCTCCACGATTT
CGCCACCAGGAACACAAACAGCTTCTAAAGAGGGGTCAGCATTTTCCTGTTATAGCCGCA
AACCTTGGATCTGCTGTTAAGGTGTTAAATAGCCAGTCAGAAAGTTCTGCTTTAACCAAC
CAACAGCCCCAAAATAACGGAGAGGTGCAGAACAGCAAAAACCAGTCCGATATAAACCAC
AGTACTTCCGGATCCCATTATGAAAATTCCCAGCGGGGACCTGTGTCTTCTACAAGTGAT
TCTAGCACAAACTGTAAGAACGCTGTTGTAAGTGACTTGTCGGAAAAAGAAGCATGGCCT
TCAGCCCCTGGCTCCGATCCGGAGTTGGCTTCCGAATGTATGGATGCCGATTCTGCCTCT
AGTTCTGAATCAGAGAGAAACATCACTATCATGGCCTCAGGGAACACAGGTGGTGAAAAA
GATGGCCTTAGGAATAGCACTGGACTTGGTTCTCAAAACAAATTCGTAGTTGGCAGCTCC
AGTAATAATGTGGGACATGGAAGTTCTACTGGGCCATGGGGTTTTTCCCATGGAGCCATA
ATAAGCACATGTCAGGTCAGTGTGGATGCTCCCGAAAGCAAATCTGAATCTAGCAACAAT
AGAATGAATGCTTGGGGCACTGTAAGTTCTTCATCAAATGGAGGGTTAAATCCATCCACT
CTGAACTCAGCTAGTAACCATGGTGCCTGGCCAGTATTAGAGAACAATGGACTTGCCCTA
AAGGGGCCTGTAGGGTCTGGTAGTTCCGGCATTAATATTCAGTGCAGTACTATAGGCCAG
ATGCCTAACAACCAGAGTATTAACTCTAAAGTGAGTGGTGGTTCTACCCATGGCACATGG
GGATCCCTGCAGGAAACTTGTGAATCTGAAGTAAGTGGTACCCAGAAGGTTTCATTCAGT
GGTCAGCCCCAGAATATTACAACTGAAATGACTGGACCTAATAATACTACTAACTTCATG
ACCTCCAGTTTACCAAACTCCGGTTCCGTGCAGAATAATGAGCTGCCTAGTAGTAACACA
GGGGCTTGGCGGGTGAGCACAATGAATCATCCTCAGATGCAAGCTCCATCAGGTATGAAT
GGCACTTCCCTTTCTCACCTTAGCAATGGCGAGTCCAAAAGTGGAGGCTCTTATGGTACT
ACATGGGGTGCCTATGGTTCTAATTACTCTGGCGACAAATGTTCAGGGCCTAATGGCCAA
GCTAATGGCGACACTGTGAATGCAACCCTAATGCAGCCTGGCGTAAATGGTCCTATGGGC
ACTAACTTTCAAGTTAACACAAATAAAGGAGGTGGTGTGTGGGAATCTGGTGCAGCAAAC
TCCCAGAGTACATCTTGGGGAAGTGGAAATGGCGCAAATTCTGGAGGCAGTCGGAGGGGC
TGGGGGACTCCTGCACAAAACACTGGCACTAATTTACCCAGCGTTGAATGGAACAAACTT
CCTAGCAACCAGCATTCCAACGATAGTGCAAATGGGAATGGTAAAACGTTTACAAATGGA
TGGAAATCTACTGAGGAAGAGGATCAGGGTTCTGCCACATCTCAGACAAATGAGCAAAGC
AGCGTGTGGGCTAAAACCGGAGGTACTGTCGAGAGTGATGGAAGTACAGAATCCACTGGA
CGCCTTGAGGAAAAAGGAACTGGCGAAAGTCAGAGTAGAGACAGAAGGAAAATTGATCAG
CACACATTACTCCAGAGCATTGTAAACAGAACTGACTTAGATCCACGTGTCCTGTCCAAC
TCAGGTTGGGGCCAGACTCCTATTAAGCAGAATACCGCCTGGGATACAGAGACATCACCT
AGAGGGGAGCGAAAGACTGACAATGGGACAGAGGCCTGGGGAAGCTCTGCAACACAGACT
TTTAACTCAGGGGCATGTATAGACAAGACTAGCCCTAATGGTAATGATACCTCATCTGTA
TCAGGGTGGGGCGACCCCAAACCTGCCCTGAGGTGGGGTGATTCCAAAGGCTCAAACTGC
CAGGGCGGCTGGGAAGATGATTCTGCTGCTACAGGCATGGTCAAGAGCAATCAGTGGGGG
AATTGCAAAGAGGAGAAGGCTGCTTGGAATGACTCGCAAAAGAATAAACAAGGATGGGGT
GACGGACAAAAATCATCACAAGGGTGGTCTGTTTCTGCTAGTGATAACTGGGGAGAAACT
TCAAGGAATAACCACTGGGGTGAGGCCAATAAGAAATCCAGCTCAGGAGGTAGTGACAGT
GACAGGTCCGTTTCCGGCTGGAACGAACTTGGTAAGACTAGTTCTTTCACCTGGGGGAAC
AACATAAATCCAAATAATTCATCAGGATGGGATGAATCCTCTAAACCTACTCCTTCCCAG
GGTTGGGGAGATCCTCCAAAGTCCAATCAGTCTCTGGGATGGGGAGACTCGTCAAAGCCC
GTCAGCTCTCCAGACTGGAATAAGCAACAAGACATTGTTGGATCGTGGGGAATCCCACCA
GCTACCGGCAAACCTCCTGGCACAGGATGGCTCGGGGGACCTATACCAGCGCCAGCAAAG
GAGGAAGAACCCACTGGCTGGGAGGAACCATCCCCCGAATCTATACGTCGCAAAATGGAG
ATTGATGATGGAACCTCTGCCTGGGGCGATCCAAGCAAATACAACTACAAAAATGTGAAC
ATGTGGAACAAGAACGTCCCAAATGGCAATAGCCGTTCCGACCAGCAAGCACAGGTACAT
CAGCTGCTAACGCCTGCAAGCGCCATCTCAAACAAAGAGGCAAGCAGTGGCAGCGGTTGG
GGTGAACCATGGGGCGAGCCTTCTACCCCAGCCACAACCGTGGATAATGGAACTTCAGCA
TGGGGTAAGCCCATAGACAGTGGTCCCAGTTGGGGCGAACCCATTGCTGCCGCATCCAGC
ACATCTACGTGGGGGTCCAGCTCTGTTGGTCCACAAGCATTAAGCAAGTCCGGGCCAAAA
TCTATGCAAGATGGCTGGTGTGGTGATGATATGCCATTGCCTGGAAACCGCCCCACTGGA
TGGGAAGAGGAGGAGGATGTCGAGATCGGAATGTGGAATAGTAACTCATCTCAAGAGCTG
AATTCATCTTTAAATTGGCCCCCATATACAAAGAAAATGTCATCGAAGGGTCTGAGTGGC
AAAAAACGGAGAAGGGAAAGGGGAATGATGAAAGGTGGAAACAAACAAGAGGAAGCCTGG
ATAAATCCTTTTGTTAAACAGTTTTCAAACATCAGTTTTTCGAGAGACTCACCAGAAGAG
AATGTACAAAGCAATAAGATGGACCTTTCCGGAGGAATGTTACAAGACAAACGAATGGAA
ATAGATAAACATAGCCTAAATATTGGCGATTACAATCGAACGGTCGGGAAAGGCCCTGGT
TCACGGCCTCAAATTTCCAAAGAGTCTTCTATGGAGCGCAATCCTTATTTTGATAAGGAT
GGCATTGTAGCAGATGAATCTCAAAATATGCAGTTTATGTCCTCTCAAAGCATGAAGCTT
CCTCCCTCAAATAGTGCACTGCCCAACCAGGCCCTCGGCTCTATAGCAGGGCTGGGTATG
CAAAACTTGAATTCTGTTAGACAGAACGGCAATCCCAGTATGTTTGGCGTTGGAAACACC
GCAGCACAACCCCGCGGAATGCAGCAGCCCCCCGCACAACCTCTTAGTTCATCTCAGCCT
AATCTCCGGGCTCAAGTGCCTCCTCCATTACTCTCCCCTCAGGTTCCAGTTTCATTGCTC
AAGTATGCACCAAACAACGGTGGCCTGAATCCGCTCTTTGGCCCTCAACAAGTAGCTATG
CTGAACCAGCTATCCCAGCTAAACCAGCTGTCTCAGATCTCCCAGTTACAGCGATTGTTA
GCCCAACAACAAAGGGCCCAGAGTCAGAGAAGCGTGCCTTCCGGGAACAGGCCTCAACAG
GACCAGCAGGGCCGACCCCTTAGTGTACAGCAGCAAATGATGCAACAATCTCGTCAACTG
GATCCAAACCTCTTGGTGAAGCAACAGACTCCACCAAGTCAGCAACAGCCACTCCATCAA
CCCGCCATGAAGTCTTTCCTTGACAATGTCATGCCCCACACTACTCCCGAGCTGCAAAAA
GGGCCATCACCAATCAATGCTTTCAGCAACTTCCCTATAGGCTTGAACTCAAACCTGAAC
GTAAATATGGATATGAACAGCATTAAAGAGCCACAGTCAAGATTGAGAAAGTGGACGACA
GTGGACAGCATTTCTGTGAACACATCATTGGATCAAAACTCCAGCAAACATGGTGCTATT
TCAAGTGGCTTCAGGCTGGAAGAGTCCCCATTTGTTCCCTATGACTTTATGAACTCTAGT
ACCTCACCAGCGAGTCCTCCAGGTTCAATAGGAGATGGATGGCCACGTGCCAAATCGCCC
AACGGCTCTAGCAGTGTTAATTGGCCACCCGAATTTCGTCCTGGTGAGCCATGGAAAGGT
TATCCAAACATTGACCCTGAAACCGACCCTTACGTCACTCCTGGCTCTGTGATAAACAAT
CTTTCAATTAATACTGTCCGCGAAGTTGACCATCTCAGGGACAGGAACAGTGGGTCATCC
TCGTCCTTGAACACCACGCTGCCTTCAACTAGTGCCTGGTCATCTATTCGTGCCTCTAAC
TACAACGTTCCCCTGAGCAGTACAGCACAAAGTACTTCAGCCAGAAATAGTGATTCAAAA
TTGACATGGTCACCTGGTTCCGTTACAAACACCTCTCTGGCTCACGAGCTGTGGAAGGTC
CCTCTGCCACCTAAAAACATTACTGCTCCGTCCCGCCCACCTCCGGGACTGACTGGTCAG
AAGCCCCCCTTGTCCACGTGGGATAATTCTCCCCTTCGTATAGGCGGCGGGTGGGGAAAT
TCTGACGCCAGATATACCCCCGGTTCCAGCTGGGGAGAGAGTAGCTCCGGGAGAATAACA
AATTGGCTTGTTCTAAAAAACCTTACACCTCAGATCGATGGCTCAACTCTGCGCACTCTG
TGCATGCAGCACGGCCCGCTGATCACATTCCACCTGAACCTGCCTCACGGAAATGCCCTG
GTCCGCTACAGTAGCAAAGAAGAGGTAGTCAAGGCACAAAAGTCTCTGCACATGTGTGTA
CTGGGGAACACTACTATTCTTGCCGAGTTTGCCAGTGAAGAGGAAATTAGTCGTTTCTTT
GCACAAAGCCAGTCTCTCACCCCTTCCCCCGGCTGGCAATCTCTCGGCTCCAGCCAGAGT
CGGCTCGGATCCCTCGACTGTTCCCACTCATTCTCTAGCCGGACCGATCTCAATCACTGG
AATGGTGCTGGGCTGTCGGGAACTAACTGTGGAGATCTTCACGGCACTTCACTCTGGGGC
ACCCCGCATTATTCAACAAGCCTGTGGGGTCCCCCAAGCTCTAGCGACCCCCGAGGAATT
AGCAGCCCATCTCCCATTAACGCTTTCCTTTCTGTTGACCACCTGGGTGGGGGTGGAGAG
TCCATG
Protein sequence predicted based on nucleotide sequence
MRELEAKATKDVERNLSRDLVQEEEQLMEEKKKKKDDKKKKEAAQKKATEQKIKVPEQIK PSVSQPQPANSNNGTSTATSTNNNAKRATANNQQPQQQQQQQQPQQQQPQQQPQPQPQQQ QPQQQPQALPRYPREVPPRFRHQEHKQLLKRGQHFPVIAANLGSAVKVLNSQSESSALTN QQPQNNGEVQNSKNQSDINHSTSGSHYENSQRGPVSSTSDSSTNCKNAVVSDLSEKEAWP SAPGSDPELASECMDADSASSSESERNITIMASGNTGGEKDGLRNSTGLGSQNKFVVGSS SNNVGHGSSTGPWGFSHGAIISTCQVSVDAPESKSESSNNRMNAWGTVSSSSNGGLNPST LNSASNHGAWPVLENNGLALKGPVGSGSSGINIQCSTIGQMPNNQSINSKVSGGSTHGTW GSLQETCESEVSGTQKVSFSGQPQNITTEMTGPNNTTNFMTSSLPNSGSVQNNELPSSNT GAWRVSTMNHPQMQAPSGMNGTSLSHLSNGESKSGGSYGTTWGAYGSNYSGDKCSGPNGQ ANGDTVNATLMQPGVNGPMGTNFQVNTNKGGGVWESGAANSQSTSWGSGNGANSGGSRRG WGTPAQNTGTNLPSVEWNKLPSNQHSNDSANGNGKTFTNGWKSTEEEDQGSATSQTNEQS SVWAKTGGTVESDGSTESTGRLEEKGTGESQSRDRRKIDQHTLLQSIVNRTDLDPRVLSN SGWGQTPIKQNTAWDTETSPRGERKTDNGTEAWGSSATQTFNSGACIDKTSPNGNDTSSV SGWGDPKPALRWGDSKGSNCQGGWEDDSAATGMVKSNQWGNCKEEKAAWNDSQKNKQGWG DGQKSSQGWSVSASDNWGETSRNNHWGEANKKSSSGGSDSDRSVSGWNELGKTSSFTWGN NINPNNSSGWDESSKPTPSQGWGDPPKSNQSLGWGDSSKPVSSPDWNKQQDIVGSWGIPP ATGKPPGTGWLGGPIPAPAKEEEPTGWEEPSPESIRRKMEIDDGTSAWGDPSKYNYKNVN MWNKNVPNGNSRSDQQAQVHQLLTPASAISNKEASSGSGWGEPWGEPSTPATTVDNGTSA WGKPIDSGPSWGEPIAAASSTSTWGSSSVGPQALSKSGPKSMQDGWCGDDMPLPGNRPTG WEEEEDVEIGMWNSNSSQELNSSLNWPPYTKKMSSKGLSGKKRRRERGMMKGGNKQEEAW INPFVKQFSNISFSRDSPEENVQSNKMDLSGGMLQDKRMEIDKHSLNIGDYNRTVGKGPG SRPQISKESSMERNPYFDKDGIVADESQNMQFMSSQSMKLPPSNSALPNQALGSIAGLGM QNLNSVRQNGNPSMFGVGNTAAQPRGMQQPPAQPLSSSQPNLRAQVPPPLLSPQVPVSLL KYAPNNGGLNPLFGPQQVAMLNQLSQLNQLSQISQLQRLLAQQQRAQSQRSVPSGNRPQQ DQQGRPLSVQQQMMQQSRQLDPNLLVKQQTPPSQQQPLHQPAMKSFLDNVMPHTTPELQK GPSPINAFSNFPIGLNSNLNVNMDMNSIKEPQSRLRKWTTVDSISVNTSLDQNSSKHGAI SSGFRLEESPFVPYDFMNSSTSPASPPGSIGDGWPRAKSPNGSSSVNWPPEFRPGEPWKG YPNIDPETDPYVTPGSVINNLSINTVREVDHLRDRNSGSSSSLNTTLPSTSAWSSIRASN YNVPLSSTAQSTSARNSDSKLTWSPGSVTNTSLAHELWKVPLPPKNITAPSRPPPGLTGQ KPPLSTWDNSPLRIGGGWGNSDARYTPGSSWGESSSGRITNWLVLKNLTPQIDGSTLRTL CMQHGPLITFHLNLPHGNALVRYSSKEEVVKAQKSLHMCVLGNTTILAEFASEEEISRFF AQSQSLTPSPGWQSLGSSQSRLGSLDCSHSFSSRTDLNHWNGAGLSGTNCGDLHGTSLWG TPHYSTSLWGPPSSSDPRGISSPSPINAFLSVDHLGGGGESM
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
