Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
Codon optimized
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
None
Description:
None
Comments:
Gene synthesis by Gen9. Codon optimized
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00744486
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 6108nts
Open reading frame: 1 to 6108
ATGGCAGCAGTTACAATGTCAGTACCGGGTCGCAAAGCGCCTCCACGACCTGGGCCGGTC
CCAGAAGCGGCTCAACCATTCTTATTCACTCCGAGAGGCCCATCTGCCGGGGGTGGTCCC
GGATCTGGCACAAGTCCGCAGGTTGAGTGGACCGCTAGACGGCTCGTCTGGGTCCCTTCG
GAACTTCACGGGTTTGAAGCGGCAGCACTCCGGGACGAGGGTGAGGAGGAGGCAGAAGTA
GAACTGGCAGAATCTGGTCGCCGGTTACGACTCCCGAGAGATCAGATCCAACGGATGAAT
CCCCCCAAGTTCAGTAAAGCGGAGGACATGGCGGAACTGACCTGCCTAAATGAAGCATCC
GTCCTTCACAATTTGAGAGAGCGTTATTACTCGGGTCTAATCTACACCTACAGTGGTCTG
TTCTGTGTCGTTATAAATCCATATAAGCAGCTTCCCATTTACACAGAAGCCATCGTGGAA
ATGTACCGGGGGAAAAAGAGACATGAGGTGCCACCCCACGTCTACGCAGTCACGGAAGGG
GCGTACAGGTCAATGTTACAGGACCGCGAGGACCAAAGCATACTATGCACGGGCGAAAGT
GGGGCGGGGAAGACTGAGAATACAAAAAAGGTAATCCAATACTTGGCCCATGTGGCTTCT
AGCCCCAAGGGAAGAAAGGAACCAGGAGTGCCTGCGTCAGTATCCACTGTCTCCTATGGT
GAATTAGAACGCCAGCTCCTACAGGCTAACCCGATCCTCGAGGCATTCGGAAACGCAAAG
ACCGTTAAGAATGATAACTCAAGCCGCTTTGGTAAATTCATAAGGATTAACTTTGACGTC
GCCGGCTATATCGTAGGGGCCAATATAGAGACGTACCTGCTGGAAAAATCCCGTGCCATA
AGACAAGCTAAAGACGAATGTTCATTTCACATTTTTTATCAATTATTGGGCGGGGCTGGT
GAGCAGCTCAAAGCGGATTTGTTGCTCGAACCTTGTAGCCATTATAGATTTCTCACCAAC
GGACCGTCGTCATCCCCGGGGCAGGAAAGGGAGCTGTTTCAGGAAACACTTGAATCGCTG
AGAGTCCTAGGGTTCTCCCACGAGGAAATAATTTCTATGCTACGAATGGTGTCCGCCGTC
CTGCAATTCGGTAATATAGCCCTCAAGCGAGAGCGTAATACGGATCAAGCTACGATGCCA
GACAATACTGCCGCGCAGAAACTCTGCAGACTTCTAGGATTGGGGGTTACAGACTTCAGC
AGGGCCTTACTTACACCTCGAATAAAAGTTGGGCGCGACTATGTGCAAAAGGCTCAGACT
AAGGAACAGGCGGATTTTGCGCTGGAGGCACTCGCAAAGGCAACCTACGAACGGCTATTT
CGTTGGTTGGTACTGCGGCTAAACAGGGCACTGGACCGATCGCCGCGACAAGGGGCCTCC
TTCTTAGGTATCTTAGACATTGCGGGCTTCGAGATTTTCCAATTAAACTCCTTTGAGCAG
TTATGTATCAACTACACGAACGAAAAATTACAACAGCTATTCAACCACACAATGTTCGTG
TTAGAGCAGGAAGAGTATCAACGGGAGGGCATACCCTGGACATTTCTCGACTTCGGTTTA
GACTTGCAACCGTGTATCGACCTTATAGAACGTCCAGCCAATCCCCCTGGGTTACTCGCT
TTATTGGACGAGGAGTGTTGGTTTCCCAAGGCAACGGATAAGTCTTTTGTCGAAAAAGTT
GCACAGGAGCAAGGTGGACATCCTAAATTTCAAAGGCCCCGACATTTGCGCGATCAGGCT
GACTTTTCTGTATTGCACTACGCAGGAAAAGTCGATTATAAAGCTAATGAGTGGTTAATG
AAAAATATGGATCCGCTCAATGACAACGTCGCAGCATTGCTTCACCAAAGTACTGATCGT
TTAACTGCCGAGATATGGAAGGACGAGCACGGCGGTTTCCAACAGTTCTCGTTCCTTGGC
TCATTCCCACCAAGCCCGCCAGGATCAGCTGAGCGGTGTTCGAGTGCGATAAGCCCTCCG
GGGGTTGAGGGCATCGTCGGCCTAGAACAAGTGTCGTCGCTCGGCGACGGGCCGCCAGGG
GGCCGCCCTCGTAGAGGGATGTTTCGCACGGTGGGTCAGTTATATAAGGAGTCCCTATCT
CGCCTCATGGCTACGTTATCCAACACCAACCCCTCCTTTGTCCGGTGTATTGTGCCGAAC
CATGAAAAGAGAGCGGGTAAGCTTGAGCCCAGATTGGTACTAGATCAGCTTAGGTGCAAC
GGTGTACTTGAAGGCATACGGATTTGCCGCCAAGGATTTCCCAATAGAATCTTATTCCAG
GAATTCCGACAGCGCTATGAAATACTAACGCCCAATGCAATTCCTAAGGGATTTATGGAT
GGGAAACAAGCATGCGAGAAGATGATACAAGCGCTGGAACTGGACCCTAACTTATATCGA
GTGGGGCAGTCGAAAATTTTTTTCCGTGCGGGGGTACTGGCCCAGCTAGAAGAAGAACGA
GACTTAAAGGTAACGGATATCATCGTCTCGTTTCAGGCTGCAGCACGTGGTTATCTAGCC
CGAAGGGCATTTCAGAAGCGTCAGCAGCAACAGTCTGCGCTGCGGGTAATGCAAAGGAAC
TGCGCAGCGTACCTCAAGCTCCGGCACTGGCAGTGGTGGCGTCTGTTCACGAAGGTCAAA
CCTTTGCTTCAGGTCACTAGACAGGACGAGGTACTGCAAGCAAGGGCCCAAGAACTCCAA
AAGGTTCAGGAGCTCCAACAGCAAAGTGCAAGAGAGGTGGGTGAGCTACAGGGACGGGTT
GCCCAGCTCGAAGAGGAGCGAGCTAGGCTTGCCGAACAGTTACGTGCTGAGGCAGAGCTA
TGTGCGGAAGCTGAGGAGACTAGGGGTCGCTTGGCAGCCCGTAAACAGGAGTTAGAACTC
GTGGTTAGTGAACTCGAAGCTCGCGTAGGGGAAGAAGAGGAGTGCTCGAGACAAATGCAA
ACCGAAAAGAAGAGATTGCAACAGCATATTCAGGAACTAGAGGCACATCTAGAAGCAGAG
GAGGGTGCTCGCCAGAAACTACAGCTGGAGAAGGTAACCACGGAGGCGAAAATGAAGAAG
TTTGAAGAGGATTTGCTACTCCTCGAAGACCAAAATTCCAAACTCTCCAAAGAACGAAAG
TTACTAGAAGATCGTCTTGCAGAATTCAGTAGCCAAGCAGCGGAAGAGGAGGAGAAAGTG
AAATCCCTGAATAAATTGCGATTGAAATACGAGGCTACGATTGCCGACATGGAAGATCGA
TTGCGAAAGGAGGAAAAGGGCCGACAGGAACTTGAGAAATTGAAAAGGCGTCTGGATGGA
GAAAGCAGCGAGCTGCAGGAGCAGATGGTGGAACAACAGCAGCGTGCCGAAGAATTGCGT
GCACAGCTCGGTCGCAAGGAGGAGGAATTACAGGCAGCTCTAGCTAGGGCGGAAGATGAG
GGTGGTGCGAGAGCACAGTTGCTAAAGTCCTTGCGGGAGGCGCAGGCTGCTCTTGCTGAA
GCCCAGGAAGATCTAGAGTCAGAACGAGTGGCACGCACTAAAGCTGAGAAACAGCGGAGG
GACTTAGGTGAAGAGCTTGAAGCTCTAAGAGGTGAACTGGAGGATACTTTAGACTCCACA
AACGCACAACAAGAGTTGAGATCTAAGAGAGAACAAGAAGTTACGGAACTAAAAAAAACG
TTGGAGGAAGAAACTCGCATACATGAGGCCGCTGTACAGGAGCTAAGACAGCGGCATGGT
CAAGCACTAGGCGAACTGGCGGAGCAACTTGAACAAGCACGTCGCGGCAAGGGTGCATGG
GAAAAGACCCGATTGGCCCTGGAGGCGGAAGTCTCTGAGTTGCGAGCTGAGCTCTCATCC
CTACAGACTGCAAGGCAAGAGGGAGAGCAGCGCCGAAGACGACTGGAGTTACAACTACAG
GAGGTTCAAGGACGTGCAGGAGACGGTGAAAGAGCACGTGCAGAGGCGGCAGAAAAGCTG
CAACGAGCACAGGCAGAACTGGAAAATGTATCTGGGGCGCTAAATGAGGCTGAGTCTAAG
ACGATACGTTTATCTAAGGAATTATCGTCAACGGAGGCCCAGTTACATGATGCTCAAGAG
TTATTACAAGAGGAAACTAGAGCCAAACTAGCCCTCGGTAGCCGTGTGCGGGCGATGGAA
GCAGAAGCAGCCGGCCTTAGAGAGCAATTAGAAGAGGAAGCCGCCGCTCGTGAGCGGGCC
GGTAGGGAGCTTCAGACGGCACAGGCGCAGTTGAGCGAGTGGCGACGGCGCCAGGAGGAA
GAGGCTGGAGCCCTTGAGGCTGGCGAAGAAGCTCGACGACGTGCGGCACGAGAAGCTGAA
GCTTTGACCCAAAGGTTGGCGGAGAAGACCGAAACAGTAGATCGGCTGGAACGCGGGCGC
AGACGGCTACAGCAGGAATTAGATGACGCAACTATGGACTTAGAACAGCAACGCCAGTTA
GTAAGTACTCTGGAGAAAAAACAGCGCAAGTTTGACCAGCTGCTGGCTGAAGAGAAGGCC
GCAGTCCTTAGGGCTGTTGAAGAGCGGGAAAGGGCGGAGGCCGAGGGGAGAGAACGCGAG
GCCCGAGCCCTATCCTTAACTCGGGCTCTAGAGGAAGAACAGGAGGCGCGGGAAGAGTTA
GAGAGACAGAACCGTGCGCTCCGGGCCGAGTTGGAAGCCTTACTATCGAGTAAGGATGAC
GTGGGAAAGAGCGTGCACGAATTGGAACGAGCGTGTCGCGTCGCAGAGCAGGCAGCCAAC
GACCTGCGAGCACAAGTAACCGAACTTGAAGACGAGCTAACAGCGGCTGAGGATGCTAAG
TTACGATTGGAGGTTACCGTCCAAGCGCTCAAAACTCAACACGAGCGAGATCTCCAAGGG
CGGGACGAAGCCGGTGAGGAACGACGCCGTCAGCTTGCTAAACAACTACGCGACGCCGAG
GTAGAAAGAGACGAGGAACGCAAACAAAGAACACTCGCGGTAGCTGCAAGAAAGAAGCTG
GAGGGGGAGCTAGAGGAGTTAAAAGCACAGATGGCTAGTGCCGGGCAAGGGAAAGAAGAA
GCGGTAAAACAGTTGCGTAAGATGCAGGCCCAGATGAAAGAGTTGTGGCGGGAAGTAGAG
GAGACAAGAACCAGCAGAGAGGAGATCTTCAGTCAAAACCGGGAGAGTGAAAAGCGTCTG
AAAGGGTTAGAGGCCGAAGTGTTGCGTCTACAAGAAGAATTAGCCGCGTCAGACCGAGCC
AGAAGGCAGGCGCAACAAGATCGTGACGAGATGGCCGATGAGGTAGCAAACGGTAACTTA
TCCAAAGCCGCCATTTTGGAAGAAAAACGTCAGTTAGAGGGAAGATTAGGTCAACTTGAG
GAAGAGCTGGAAGAGGAACAGTCAAACAGTGAACTACTCAACGATCGCTATCGTAAATTA
CTACTCCAAGTGGAGAGTCTGACGACCGAACTCTCCGCAGAAAGGTCGTTCTCTGCAAAG
GCCGAATCCGGGAGGCAACAGTTGGAACGGCAAATACAAGAACTTAGGGGGAGGCTTGGA
GAAGAGGACGCCGGAGCACGGGCACGTCATAAGATGACTATCGCGGCGTTGGAATCAAAG
CTAGCGCAAGCCGAAGAGCAACTCGAGCAAGAGACTAGAGAGAGAATATTATCTGGAAAA
CTTGTTCGCCGGGCGGAAAAGAGGTTGAAGGAAGTCGTTCTGCAGGTCGAGGAGGAGCGC
AGGGTCGCAGACCAGCTCAGGGACCAGCTTGAAAAAGGAAACCTCAGAGTTAAGCAATTA
AAGCGACAACTGGAAGAAGCCGAGGAAGAAGCAAGTCGCGCACAGGCCGGCAGGCGACGG
TTACAAAGGGAATTGGAGGATGTCACTGAATCCGCTGAATCGATGAATAGGGAGGTAACG
ACGCTCCGAAATCGTCTAAGGAGAGGGCCTTTAACATTCACGACTCGAACCGTACGTCAG
GTATTCAGGCTCGAGGAAGGTGTAGCATCGGATGAAGAGGCGGAGGAAGCGCAGCCGGGC
AGCGGTCCGAGTCCAGAGCCTGAGGGCTCACCGCCGGCGCACCCTCAA
Protein sequence predicted based on nucleotide sequence
MAAVTMSVPGRKAPPRPGPVPEAAQPFLFTPRGPSAGGGPGSGTSPQVEWTARRLVWVPS ELHGFEAAALRDEGEEEAEVELAESGRRLRLPRDQIQRMNPPKFSKAEDMAELTCLNEAS VLHNLRERYYSGLIYTYSGLFCVVINPYKQLPIYTEAIVEMYRGKKRHEVPPHVYAVTEG AYRSMLQDREDQSILCTGESGAGKTENTKKVIQYLAHVASSPKGRKEPGVPASVSTVSYG ELERQLLQANPILEAFGNAKTVKNDNSSRFGKFIRINFDVAGYIVGANIETYLLEKSRAI RQAKDECSFHIFYQLLGGAGEQLKADLLLEPCSHYRFLTNGPSSSPGQERELFQETLESL RVLGFSHEEIISMLRMVSAVLQFGNIALKRERNTDQATMPDNTAAQKLCRLLGLGVTDFS RALLTPRIKVGRDYVQKAQTKEQADFALEALAKATYERLFRWLVLRLNRALDRSPRQGAS FLGILDIAGFEIFQLNSFEQLCINYTNEKLQQLFNHTMFVLEQEEYQREGIPWTFLDFGL DLQPCIDLIERPANPPGLLALLDEECWFPKATDKSFVEKVAQEQGGHPKFQRPRHLRDQA DFSVLHYAGKVDYKANEWLMKNMDPLNDNVAALLHQSTDRLTAEIWKDEHGGFQQFSFLG SFPPSPPGSAERCSSAISPPGVEGIVGLEQVSSLGDGPPGGRPRRGMFRTVGQLYKESLS RLMATLSNTNPSFVRCIVPNHEKRAGKLEPRLVLDQLRCNGVLEGIRICRQGFPNRILFQ EFRQRYEILTPNAIPKGFMDGKQACEKMIQALELDPNLYRVGQSKIFFRAGVLAQLEEER DLKVTDIIVSFQAAARGYLARRAFQKRQQQQSALRVMQRNCAAYLKLRHWQWWRLFTKVK PLLQVTRQDEVLQARAQELQKVQELQQQSAREVGELQGRVAQLEEERARLAEQLRAEAEL CAEAEETRGRLAARKQELELVVSELEARVGEEEECSRQMQTEKKRLQQHIQELEAHLEAE EGARQKLQLEKVTTEAKMKKFEEDLLLLEDQNSKLSKERKLLEDRLAEFSSQAAEEEEKV KSLNKLRLKYEATIADMEDRLRKEEKGRQELEKLKRRLDGESSELQEQMVEQQQRAEELR AQLGRKEEELQAALARAEDEGGARAQLLKSLREAQAALAEAQEDLESERVARTKAEKQRR DLGEELEALRGELEDTLDSTNAQQELRSKREQEVTELKKTLEEETRIHEAAVQELRQRHG QALGELAEQLEQARRGKGAWEKTRLALEAEVSELRAELSSLQTARQEGEQRRRRLELQLQ EVQGRAGDGERARAEAAEKLQRAQAELENVSGALNEAESKTIRLSKELSSTEAQLHDAQE LLQEETRAKLALGSRVRAMEAEAAGLREQLEEEAAARERAGRELQTAQAQLSEWRRRQEE EAGALEAGEEARRRAAREAEALTQRLAEKTETVDRLERGRRRLQQELDDATMDLEQQRQL VSTLEKKQRKFDQLLAEEKAAVLRAVEERERAEAEGREREARALSLTRALEEEQEAREEL ERQNRALRAELEALLSSKDDVGKSVHELERACRVAEQAANDLRAQVTELEDELTAAEDAK LRLEVTVQALKTQHERDLQGRDEAGEERRRQLAKQLRDAEVERDEERKQRTLAVAARKKL EGELEELKAQMASAGQGKEEAVKQLRKMQAQMKELWREVEETRTSREEIFSQNRESEKRL KGLEAEVLRLQEELAASDRARRQAQQDRDEMADEVANGNLSKAAILEEKRQLEGRLGQLE EELEEEQSNSELLNDRYRKLLLQVESLTTELSAERSFSAKAESGRQQLERQIQELRGRLG EEDAGARARHKMTIAALESKLAQAEEQLEQETRERILSGKLVRRAEKRLKEVVLQVEEER RVADQLRDQLEKGNLRVKQLKRQLEEAEEEASRAQAGRRRLQRELEDVTESAESMNREVT TLRNRLRRGPLTFTTRTVRQVFRLEEGVASDEEAEEAQPGSGPSPEPEGSPPAHPQ
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
NGS Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
