Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
Codon optimized
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
None
Description:
None
Comments:
Gene synthesis by Gen9. Codon optimized
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00744490
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 4014nts
Open reading frame: 1 to 4014
ATGGCTAGCCCTCCACGGCACGGACCGCCAGGGCCAGCAAGCGGAGATGGTCCTAACCTC
AACAATAATAACAATAACAACAACCATAGCGTGAGAAAGTGCGGCTATCTTCGAAAGCAG
AAGCATGGACACAAGAGATTTTTCGTGCTGCGAGGACCTGGCGCTGGCGGCGATGAAGCT
ACTGCTGGCGGGGGTTCTGCTCCTCAACCACCAAGGCTCGAGTACTATGAGAGCGAAAAA
AAGTGGAGAAGCAAAGCAGGCGCACCTAAAAGGGTGATTGCTCTCGACTGTTGTCTGAAC
ATCAACAAGAGAGCCGATGCCAAGCACAAATACCTGATCGCTCTCTACACCAAGGATGAA
TACTTTGCCGTTGCCGCCGAGAACGAGCAGGAACAAGAGGGCTGGTACAGAGCTCTAACC
GACCTGGTCAGCGAAGGCAGAGCAGCAGCAGGAGACGCTCCTCCCGCCGCAGCACCCGCA
GCATCTTGTTCAGCCTCACTGCCCGGAGCTTTGGGTGGCTCTGCAGGCGCTGCTGGTGCC
GAGGACAGCTACGGACTGGTGGCTCCCGCAACAGCCGCATACCGTGAGGTATGGCAGGTA
AACCTGAAGCCTAAGGGTCTGGGACAGAGCAAGAACCTGACAGGGGTGTACAGACTGTGC
CTGTCTGCTCGCACCATTGGATTCGTAAAGCTCAATTGTGAACAGCCTTCTGTGACACTG
CAGCTAATGAACATTAGACGCTGCGGTCACTCTGACAGCTTCTTTTTCATCGAGGTGGGC
AGATCAGCCGTCACAGGACCCGGCGAACTTTGGATGCAGGCTGATGACTCTGTGGTGGCT
CAGAACATCCACGAGACAATTCTGGAGGCCATGAAAGCACTTAAAGAGCTTTTTGAGTTC
CGGCCTCGCTCAAAGAGCCAATCCTCGGGATCTTCTGCTACTCATCCCATTAGCGTTCCT
GGAGCTAGACGCCATCACCATCTGGTCAACCTTCCCCCCAGTCAGACAGGACTTGTGAGA
CGATCTAGAACAGACAGCTTGGCCGCTACCCCTCCGGCTGCTAAGTGCTCATCTTGTAGA
GTAAGAACTGCCAGTGAGGGCGACGGCGGCGCTGCAGCGGGAGCTGCGGCCGCTGGTGCT
AGGCCTGTTTCTGTTGCTGGAAGTCCCCTTAGTCCTGGACCGGTGAGAGCACCTCTGAGT
AGATCTCACACTCTGAGCGGCGGATGCGGCGGAAGAGGAAGCAAGGTTGCACTTCTGCCA
GCAGGGGGCGCACTGCAACACTCACGCTCTATGTCTATGCCCGTGGCACATTCTCCGCCC
GCTGCCACAAGCCCCGGTTCCCTGTCTTCTAGTAGCGGACATGGCTCAGGCTCATACCCG
CCGCCACCCGGCCCTCACCCACCACTACCTCATCCTCTGCATCACGGCCCAGGCCAAAGG
CCTTCCAGTGGAAGCGCCTCCGCTTCTGGATCCCCCAGTGATCCCGGATTCATGTCCCTG
GACGAGTACGGCTCCAGTCCAGGCGATCTTCGCGCCTTCTGCAGTCATCGAAGCAATACA
CCCGAGTCCATCGCTGAGACACCTCCTGCACGAGACGGTGGCGGAGGAGGTGAATTCTAC
GGATACATGACAATGGACAGGCCATTAAGCCACTGTGGACGATCCTACCGACGGGTTTCA
GGGGACGCTGCACAGGATCTGGACCGAGGTCTGAGAAAGAGAACATACTCCCTGACAACA
CCAGCCCGGCAGCGGCCTGTTCCACAGCCCTCCTCTGCTTCTCTGGATGAATATACCCTT
ATGCGGGCCACATTTTCTGGCAGCGCAGGCCGATTATGTCCTTCCTGCCCCGCTTCATCT
CCAAAGGTGGCCTACCACCCTTACCCAGAGGATTACGGAGACATCGAAATCGGCTCACAT
AGGAGCTCTAGCAGCAACCTAGGGGCAGACGACGGCTACATGCCTATGACTCCAGGAGCA
GCACTCGCTGGCAGTGGGAGTGGCAGCTGTAGGTCCGACGACTATATGCCCATGAGCCCC
GCCTCCGTGTCCGCCCCAAAGCAGATCTTGCAGCCTAGAGCTGCAGCCGCTGCAGCAGCT
GCTGTTCCTTCTGCTGGGCCTGCGGGTCCAGCACCCACCTCTGCAGCTGGCAGGACATTC
CCTGCGAGCGGGGGTGGATATAAGGCCAGTTCTCCAGCTGAGAGCTCACCTGAGGACAGT
GGGTATATGCGCATGTGGTGTGGTTCCAAGTTGTCTATGGAGCATGCAGATGGCAAATTG
CTGCCCAACGGTGACTATCTCAACGTTTCGCCAAGCGACGCGGTTACAACTGGAACCCCT
CCCGATTTCTTTTCCGCAGCCCTGCATCCCGGCGGGGAACCACTCCGAGGCGTTCCCGGA
TGCTGCTATAGCTCTTTGCCACGCTCTTACAAAGCCCCTTACACCTGTGGCGGGGACAGC
GACCAGTACGTTCTCATGAGCTCCCCTGTGGGGCGAATCCTGGAGGAGGAACGTCTGGAA
CCTCAGGCCACACCAGGGCCCAGCCAGGCAGCCAGTGCCTTCGGGGCTGGCCCCACACAA
CCACCTCACCCTGTAGTTCCTTCACCTGTGCGGCCTAGCGGAGGAAGACCTGAGGGCTTT
TTGGGCCAGAGAGGCCGAGCTGTTAGACCCACACGCCTTTCCCTGGAAGGGCTGCCCAGT
CTGCCCAGCATGCACGAGTACCCATTGCCACCAGAACCCAAGAGCCCCGGAGAGTATATT
AACATCGACTTTGGCGAACCTGGAGCCAGACTGTCGCCTCCCGCACCTCCACTGTTAGCA
TCTGCTGCATCTTCTTCATCATTATTGTCCGCCAGTAGCCCAGCCTCGTCGCTGGGCTCT
GGCACCCCTGGCACCAGCAGTGATAGCAGGCAGAGATCTCCTCTCTCCGACTACATGAAC
CTCGACTTCAGTTCCCCCAAGTCTCCTAAGCCTGGCGCCCCTAGTGGCCACCCCGTTGGA
TCCTTGGATGGCCTCTTATCCCCCGAAGCCTCCTCTCCTTATCCTCCTTTGCCCCCTCGT
CCTTCCGCTTCCCCTTCATCTTCTCTACAACCGCCTCCACCTCCGCCGGCCCCAGGAGAG
CTTTATCGCCTGCCCCCAGCTTCTGCCGTAGCCACCGCCCAGGGTCCTGGCGCAGCCTCA
TCTCTGTCTTCAGACACCGGAGACAATGGTGACTACACCGAAATGGCTTTTGGAGTGGCC
GCCACACCGCCGCAACCAATTGCAGCCCCTCCGAAGCCAGAAGCTGCGCGCGTGGCCAGT
CCTACATCTGGCGTGAAAAGACTGAGCCTCATGGAGCAGGTTTCTGGAGTCGAGGCCTTC
TTACAGGCCAGCCAGCCTCCTGACCCTCACAGAGGAGCTAAGGTTATCAGAGCAGATCCT
CAAGGGGGAAGACGCCGACACAGTTCCGAAACCTTCTCTTCTACAACAACAGTTACACCA
GTGTCTCCTTCCTTCGCCCACAACCCCAAGAGACACAACTCTGCCTCTGTGGAAAATGTT
TCTCTCAGGAAAAGCAGCGAGGGCGGCGTGGGAGTCGGCCCTGGAGGGGGAGATGAGCCT
CCCACCTCCCCAAGACAGTTGCAACCGGCACCCCCTTTAGCACCACAAGGCCGACCTTGG
ACCCCGGGTCAGCCCGGAGGCTTGGTCGGTTGTCCTGGGAGTGGTGGATCTCCCATGCGC
AGAGAGACATCTGCCGGATTCCAGAATGGACTCAACTACATCGCAATTGATGTGAGGGAA
GAGCCTGGGCTGCCACCCCAGCCACAGCCGCCTCCTCCACCACTTCCTCAGCCAGGAGAC
AAGAGTTCCTGGGGAAGAACCCGAAGCTTAGGGGGTCTTATTAGCGCTGTTGGTGTCGGA
AGCACCGGTGGAGGGTGCGGAGGACCAGGTCCCGGTGCCCTGCCTCCTGCCAACACATAC
GCCAGCATAGACTTTCTTTCCCACCATTTGAAGGAGGCCACAATCGTGAAAGAG
Protein sequence predicted based on nucleotide sequence
MASPPRHGPPGPASGDGPNLNNNNNNNNHSVRKCGYLRKQKHGHKRFFVLRGPGAGGDEA TAGGGSAPQPPRLEYYESEKKWRSKAGAPKRVIALDCCLNINKRADAKHKYLIALYTKDE YFAVAAENEQEQEGWYRALTDLVSEGRAAAGDAPPAAAPAASCSASLPGALGGSAGAAGA EDSYGLVAPATAAYREVWQVNLKPKGLGQSKNLTGVYRLCLSARTIGFVKLNCEQPSVTL QLMNIRRCGHSDSFFFIEVGRSAVTGPGELWMQADDSVVAQNIHETILEAMKALKELFEF RPRSKSQSSGSSATHPISVPGARRHHHLVNLPPSQTGLVRRSRTDSLAATPPAAKCSSCR VRTASEGDGGAAAGAAAAGARPVSVAGSPLSPGPVRAPLSRSHTLSGGCGGRGSKVALLP AGGALQHSRSMSMPVAHSPPAATSPGSLSSSSGHGSGSYPPPPGPHPPLPHPLHHGPGQR PSSGSASASGSPSDPGFMSLDEYGSSPGDLRAFCSHRSNTPESIAETPPARDGGGGGEFY GYMTMDRPLSHCGRSYRRVSGDAAQDLDRGLRKRTYSLTTPARQRPVPQPSSASLDEYTL MRATFSGSAGRLCPSCPASSPKVAYHPYPEDYGDIEIGSHRSSSSNLGADDGYMPMTPGA ALAGSGSGSCRSDDYMPMSPASVSAPKQILQPRAAAAAAAAVPSAGPAGPAPTSAAGRTF PASGGGYKASSPAESSPEDSGYMRMWCGSKLSMEHADGKLLPNGDYLNVSPSDAVTTGTP PDFFSAALHPGGEPLRGVPGCCYSSLPRSYKAPYTCGGDSDQYVLMSSPVGRILEEERLE PQATPGPSQAASAFGAGPTQPPHPVVPSPVRPSGGRPEGFLGQRGRAVRPTRLSLEGLPS LPSMHEYPLPPEPKSPGEYINIDFGEPGARLSPPAPPLLASAASSSSLLSASSPASSLGS GTPGTSSDSRQRSPLSDYMNLDFSSPKSPKPGAPSGHPVGSLDGLLSPEASSPYPPLPPR PSASPSSSLQPPPPPPAPGELYRLPPASAVATAQGPGAASSLSSDTGDNGDYTEMAFGVA ATPPQPIAAPPKPEAARVASPTSGVKRLSLMEQVSGVEAFLQASQPPDPHRGAKVIRADP QGGRRRHSSETFSSTTTVTPVSPSFAHNPKRHNSASVENVSLRKSSEGGVGVGPGGGDEP PTSPRQLQPAPPLAPQGRPWTPGQPGGLVGCPGSGGSPMRRETSAGFQNGLNYIAIDVRE EPGLPPQPQPPPPPLPQPGDKSSWGRTRSLGGLISAVGVGSTGGGCGGPGPGALPPANTY ASIDFLSHHLKEATIVKE
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
