Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
Codon optimized
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
None
Description:
None
Comments:
Gene synthesis by Gen9. Codon optimized
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00744553
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 4398nts
Open reading frame: 1 to 4398
ATGATGAGCTTTGTGCAAAAGGGGAGCTGGCTGCTTCTCGCTCTGCTTCATCCCACTATT
ATTTTGGCACAACAGGAAGCTGTTGAAGGAGGATGTTCCCATCTTGGTCAGTCTTATGCG
GATAGAGATGTCTGGAAGCCAGAACCATGCCAAATATGTGTCTGTGACTCAGGATCCGTT
CTCTGCGATGACATAATATGTGACGATCAAGAATTAGACTGCCCCAACCCAGAAATTCCA
TTTGGAGAATGTTGTGCAGTTTGCCCACAGCCTCCAACTGCTCCTACTCGCCCTCCTAAT
GGTCAAGGACCTCAAGGCCCCAAGGGCGACCCAGGACCACCCGGTATTCCGGGGAGGAAT
GGTGACCCTGGTATTCCAGGACAACCCGGGTCACCTGGTTCTCCGGGCCCCCCTGGAATC
TGTGAATCATGCCCTACTGGTCCTCAGAACTATTCTCCCCAGTATGATTCATATGATGTC
AAGTCTGGAGTAGCAGTAGGAGGACTCGCAGGCTATCCTGGACCAGCTGGCCCCCCGGGC
CCTCCCGGTCCGCCAGGTACATCTGGTCATCCTGGTTCCCCCGGATCTCCAGGATACCAA
GGTCCCCCCGGTGAACCTGGGCAAGCCGGTCCTTCAGGCCCTCCAGGACCTCCTGGTGCT
ATAGGTCCATCTGGTCCGGCTGGGAAAGATGGAGAATCAGGTAGGCCCGGACGACCAGGT
GAGCGAGGATTACCTGGACCTCCAGGTATCAAAGGTCCAGCCGGGATACCTGGATTCCCC
GGCATGAAAGGACACAGAGGCTTCGATGGCCGCAATGGAGAGAAGGGTGAAACAGGTGCT
CCTGGATTAAAGGGTGAGAATGGTCTTCCAGGCGAAAATGGAGCTCCTGGTCCCATGGGT
CCAAGAGGGGCACCTGGCGAGCGAGGACGGCCCGGACTTCCTGGGGCTGCTGGTGCTCGG
GGTAATGACGGTGCTCGAGGCAGTGATGGTCAACCCGGCCCACCTGGCCCTCCTGGAACT
GCCGGATTCCCTGGATCGCCTGGGGCTAAGGGCGAAGTTGGACCTGCAGGGTCCCCTGGT
TCAAATGGTGCGCCTGGACAAAGAGGAGAACCTGGACCACAGGGACACGCTGGTGCTCAA
GGTCCTCCCGGCCCTCCTGGGATTAATGGTAGCCCTGGTGGTAAAGGCGAAATGGGTCCC
GCTGGCATTCCTGGAGCTCCGGGACTGATGGGAGCCCGCGGTCCTCCAGGGCCAGCCGGT
GCTAATGGTGCCCCCGGACTGCGAGGTGGTGCAGGCGAGCCCGGTAAGAACGGTGCCAAA
GGAGAGCCCGGACCACGTGGTGAACGCGGTGAGGCTGGGATTCCAGGTGTTCCAGGGGCT
AAAGGCGAGGATGGAAAGGATGGATCACCTGGAGAACCCGGTGCAAATGGCCTTCCAGGA
GCAGCAGGAGAAAGGGGTGCGCCGGGGTTCCGAGGACCCGCTGGACCAAATGGCATCCCA
GGGGAAAAGGGTCCTGCGGGAGAGCGTGGTGCTCCAGGCCCTGCGGGGCCCAGAGGAGCT
GCTGGGGAACCTGGCAGAGATGGCGTCCCCGGAGGTCCGGGAATGAGGGGCATGCCCGGA
AGCCCAGGCGGGCCAGGAAGTGATGGGAAACCCGGGCCTCCCGGAAGTCAAGGAGAAAGT
GGTCGACCGGGTCCACCTGGGCCATCCGGTCCCCGAGGTCAGCCTGGTGTCATGGGCTTT
CCCGGTCCTAAAGGAAATGATGGGGCTCCTGGTAAGAATGGAGAACGAGGTGGCCCTGGC
GGCCCGGGCCCACAGGGTCCTCCTGGAAAAAATGGTGAAACTGGACCTCAGGGACCCCCA
GGGCCTACGGGGCCTGGTGGAGACAAAGGAGACACAGGCCCCCCAGGTCCACAAGGATTA
CAAGGCTTGCCTGGTACAGGTGGTCCCCCGGGAGAAAATGGAAAACCTGGGGAGCCGGGA
CCAAAGGGCGATGCCGGTGCACCCGGAGCGCCAGGCGGCAAGGGTGATGCTGGGGCCCCT
GGTGAGCGTGGGCCTCCGGGATTGGCAGGGGCGCCGGGACTTAGAGGTGGAGCTGGTCCC
CCTGGGCCCGAAGGAGGAAAGGGTGCTGCTGGACCTCCCGGACCCCCCGGTGCTGCCGGT
ACTCCTGGTCTGCAAGGAATGCCTGGAGAGAGGGGAGGTCTTGGAAGTCCCGGTCCAAAG
GGTGACAAGGGTGAACCAGGCGGTCCAGGTGCTGATGGTGTCCCAGGAAAAGATGGCCCA
AGGGGTCCTACTGGCCCTATTGGTCCGCCCGGCCCCGCTGGCCAGCCTGGAGATAAGGGT
GAAGGTGGTGCCCCAGGCCTGCCAGGGATAGCTGGACCGCGTGGTAGCCCGGGTGAGAGA
GGCGAAACGGGCCCGCCAGGACCTGCTGGTTTCCCTGGTGCTCCTGGACAGAATGGTGAG
CCTGGGGGTAAAGGAGAAAGAGGGGCTCCGGGCGAGAAAGGTGAAGGAGGCCCTCCTGGA
GTTGCAGGGCCCCCTGGAGGTTCTGGGCCTGCTGGTCCTCCGGGTCCCCAAGGTGTAAAA
GGTGAACGTGGGAGTCCTGGGGGACCTGGTGCCGCTGGCTTCCCGGGTGCTCGTGGTCTG
CCTGGTCCACCGGGTAGTAATGGCAACCCAGGCCCACCCGGTCCCAGCGGTTCTCCCGGC
AAGGATGGGCCCCCGGGTCCGGCGGGTAACACTGGTGCACCTGGCAGCCCGGGAGTGTCT
GGACCTAAAGGTGATGCCGGCCAACCAGGCGAGAAGGGATCGCCGGGTGCCCAGGGACCG
CCGGGAGCGCCTGGCCCACTTGGGATTGCTGGGATCACTGGAGCACGGGGTCTTGCAGGA
CCGCCAGGCATGCCAGGTCCGAGAGGAAGCCCTGGCCCTCAAGGTGTCAAGGGTGAAAGT
GGCAAACCAGGAGCTAACGGTCTTAGTGGAGAACGTGGACCCCCTGGACCCCAGGGTCTT
CCTGGCCTGGCTGGTACAGCTGGCGAACCTGGAAGAGATGGAAACCCTGGATCAGATGGT
CTACCAGGCCGAGATGGATCTCCTGGTGGCAAGGGGGATCGTGGTGAAAATGGCTCTCCT
GGCGCCCCCGGCGCTCCTGGCCATCCAGGCCCGCCCGGTCCTGTCGGCCCAGCTGGAAAG
AGTGGTGACAGAGGAGAGAGTGGCCCTGCTGGCCCGGCTGGTGCTCCGGGTCCTGCTGGA
TCCCGAGGTGCTCCTGGGCCTCAAGGGCCACGAGGTGACAAAGGTGAAACCGGCGAACGT
GGCGCTGCTGGCATCAAAGGACATCGAGGATTCCCAGGTAACCCAGGTGCGCCAGGGTCG
CCAGGCCCTGCCGGTCAGCAGGGTGCAATCGGCAGTCCTGGACCCGCAGGCCCCAGAGGA
CCTGTTGGCCCCAGTGGACCTCCTGGCAAAGATGGAACCAGTGGACATCCAGGTCCCATT
GGACCACCGGGGCCTCGAGGTAACAGAGGTGAAAGAGGATCTGAGGGCTCCCCCGGCCAC
CCAGGGCAACCAGGACCCCCGGGACCGCCCGGTGCCCCTGGTCCTTGCTGTGGTGGTGTT
GGAGCCGCTGCTATTGCTGGTATTGGAGGTGAAAAAGCTGGCGGTTTTGCCCCGTATTAT
GGCGATGAACCAATGGATTTCAAAATCAACACCGATGAGATTATGACTTCACTCAAGTCT
GTTAATGGACAAATAGAAAGCCTCATTAGTCCTGATGGTTCTCGTAAAAACCCCGCTAGA
AACTGCAGAGACTTAAAATTCTGCCATCCTGAACTCAAGAGTGGAGAATACTGGGTGGAC
CCTAACCAAGGATGCAAATTGGATGCTATCAAGGTATTCTGTAATATGGAAACTGGGGAA
ACATGCATAAGTGCCAATCCTTTGAATGTTCCACGGAAACACTGGTGGACAGATTCTAGT
GCTGAGAAGAAACACGTTTGGTTTGGAGAGTCCATGGATGGTGGTTTTCAGTTTAGCTAC
GGCAATCCTGAACTTCCTGAAGATGTCCTTGATGTGCAGCTGGCTTTCCTTCGACTTCTA
TCCAGCCGAGCTTCCCAGAACATCACATATCACTGCAAAAATAGTATTGCATACATGGAT
CAGGCCAGTGGAAATGTAAAGAAGGCCCTGAAGCTGATGGGGTCAAATGAAGGTGAATTC
AAGGCTGAAGGAAATAGCAAATTCACCTACACAGTTCTGGAGGATGGTTGCACGAAACAC
ACTGGGGAATGGAGCAAAACAGTCTTTGAATATCGAACACGCAAGGCTGTGAGACTACCT
ATTGTAGATATTGCACCCTATGACATTGGTGGTCCCGACCAAGAATTTGGTGTGGACGTT
GGCCCTGTTTGCTTTTTA
Protein sequence predicted based on nucleotide sequence
MMSFVQKGSWLLLALLHPTIILAQQEAVEGGCSHLGQSYADRDVWKPEPCQICVCDSGSV LCDDIICDDQELDCPNPEIPFGECCAVCPQPPTAPTRPPNGQGPQGPKGDPGPPGIPGRN GDPGIPGQPGSPGSPGPPGICESCPTGPQNYSPQYDSYDVKSGVAVGGLAGYPGPAGPPG PPGPPGTSGHPGSPGSPGYQGPPGEPGQAGPSGPPGPPGAIGPSGPAGKDGESGRPGRPG ERGLPGPPGIKGPAGIPGFPGMKGHRGFDGRNGEKGETGAPGLKGENGLPGENGAPGPMG PRGAPGERGRPGLPGAAGARGNDGARGSDGQPGPPGPPGTAGFPGSPGAKGEVGPAGSPG SNGAPGQRGEPGPQGHAGAQGPPGPPGINGSPGGKGEMGPAGIPGAPGLMGARGPPGPAG ANGAPGLRGGAGEPGKNGAKGEPGPRGERGEAGIPGVPGAKGEDGKDGSPGEPGANGLPG AAGERGAPGFRGPAGPNGIPGEKGPAGERGAPGPAGPRGAAGEPGRDGVPGGPGMRGMPG SPGGPGSDGKPGPPGSQGESGRPGPPGPSGPRGQPGVMGFPGPKGNDGAPGKNGERGGPG GPGPQGPPGKNGETGPQGPPGPTGPGGDKGDTGPPGPQGLQGLPGTGGPPGENGKPGEPG PKGDAGAPGAPGGKGDAGAPGERGPPGLAGAPGLRGGAGPPGPEGGKGAAGPPGPPGAAG TPGLQGMPGERGGLGSPGPKGDKGEPGGPGADGVPGKDGPRGPTGPIGPPGPAGQPGDKG EGGAPGLPGIAGPRGSPGERGETGPPGPAGFPGAPGQNGEPGGKGERGAPGEKGEGGPPG VAGPPGGSGPAGPPGPQGVKGERGSPGGPGAAGFPGARGLPGPPGSNGNPGPPGPSGSPG KDGPPGPAGNTGAPGSPGVSGPKGDAGQPGEKGSPGAQGPPGAPGPLGIAGITGARGLAG PPGMPGPRGSPGPQGVKGESGKPGANGLSGERGPPGPQGLPGLAGTAGEPGRDGNPGSDG LPGRDGSPGGKGDRGENGSPGAPGAPGHPGPPGPVGPAGKSGDRGESGPAGPAGAPGPAG SRGAPGPQGPRGDKGETGERGAAGIKGHRGFPGNPGAPGSPGPAGQQGAIGSPGPAGPRG PVGPSGPPGKDGTSGHPGPIGPPGPRGNRGERGSEGSPGHPGQPGPPGPPGAPGPCCGGV GAAAIAGIGGEKAGGFAPYYGDEPMDFKINTDEIMTSLKSVNGQIESLISPDGSRKNPAR NCRDLKFCHPELKSGEYWVDPNQGCKLDAIKVFCNMETGETCISANPLNVPRKHWWTDSS AEKKHVWFGESMDGGFQFSYGNPELPEDVLDVQLAFLRLLSSRASQNITYHCKNSIAYMD QASGNVKKALKLMGSNEGEFKAEGNSKFTYTVLEDGCTKHTGEWSKTVFEYRTRKAVRLP IVDIAPYDIGGPDQEFGVDVGPVCFL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
NGS Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
