Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
Codon optimized
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pGW_NcoI-Eco109I
Format:
FUSION
Source:
None
Description:
None
Comments:
Gene synthesis by Gen9. Codon optimized
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00744562
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 4758nts
Open reading frame: 1 to 4758
ATGGAGACGTCTGCCTCAGCCACTGCCTCCGAGAAGCAAGAAGCCAAAAGTGGGATCCTG
GAGGCCGCTGGCTTCCCCGACCCGGGTAAAAAGGCCTCTCCTTTGGTGGTGGCTGCAGCG
GCAGCAGCAGCGGTAGCTGCCCAAGGAGTGCCGCAGCATCTCTTGCCACCATTCCATGCG
CCCCTACCGATTGACATGCGACACCAGGAAGGAAGGTACCATTACGAGCCTCATTCTGTC
CACGGTGTGCACGGGCCCCCTGCCCTCAGCGGAAGCCCTGTCATCTCTGACATCTCCTTG
ATCCGGCTTTCCCCGCACCCGGCTGGCCCTGGGGAGTCCCCCTTCAACGCCCCCCACCCG
TACGTGAACCCCCACATGGAGCACTACCTCCGTTCTGTGCACAGCAGCCCCACGCTCTCC
ATGATCTCCGCAGCCAGGGGCCTCAGCCCCGCTGATGTGGCCCAGGAGCACCTTAAGGAG
AGGGGACTGTTTGGCCTTCCTGCTCCAGGCACCACCCCCTCAGACTATTACCACCAGATG
ACCCTCGTGGCAGGCCACCCTGCGCCCTACGGGGACCTGCTGATGCAGAGCGGGGGCGCT
GCCAGCGCACCCCATCTTCACGACTACCTCAACCCCGTGGACGTGTCCCGTTTCTCCAGT
CCGCGGGTGACGCCCCGACTGAGCCGCAAGCGGGCGCTGTCCATCTCCCCACTCTCAGAC
GCCAGCCTGGACCTGCAGCGGATGATCCGCACCTCACCCAACTCGCTAGTGGCCTACATC
AACAACTCCCGAAGCAGCTCGGCGGCCAGTGGTTCCTACGGGCATCTGTCAGCGGGTGCC
CTCAGTCCAGCCTTCACCTTCCCCCACCCCATCAACCCCGTGGCCTACCAGCAGATTCTG
AGCCAGCAGAGGGGTCTGGGGTCAGCCTTTGGACACACACCACCCCTGATCCAGCCCTCA
CCCACCTTCCTGGCCCAGCAGCCCATGGCCCTCACCTCCATCAATGCCACGCCCACCCAG
CTCAGCAGCAGCAGCAACTGTCTGAGTGACACCAACCAGAACAAGCAGAGCAGTGAGTCG
GCCGTCAGCAGCACCGTCAACCCTGTCGCCATTCACAAGCGCAGCAAGGTCAAGACCGAG
CCTGAGGGCCTGCGGCCGGCCTCCCCTCTTGCTCTGACGCAGGGCCAGGTGTCTGGACAC
GGCTCATGTGGGTGTGCCCTTCCCCTCTCCCAGGAGCAGCTGGCTGACCTCAAGGAAGAT
CTGGACAGGGATGACTGTAAGCAGGAGGCTGAGGTGGTCATCTATGAGACAAACTGCCAC
TGGGAAGACTGCACCAAGGAGTACGACACCCAGGAGCAGCTGGTGCATCACATCAACAAC
GAGCACATCCACGGGGAGAAGAAGGAGTTTGTGTGCCGCTGGCAGGCCTGCACGCGGGAG
CAGAAGCCCTTCAAGGCGCAGTACATGCTGGTGGTGCACATGCGGCGACACACGGGCGAG
AAGCCCCACAAGTGCACGTTCGAGGGCTGCTCGAAGGCCTACTCCCGCCTGGAGAACCTG
AAGACACACCTGAGGTCCCACACCGGGGAGAAGCCATATGTGTGTGAGCACGAGGGCTGC
AACAAAGCCTTCTCCAACGCCTCGGACCGCGCCAAGCACCAGAATCGCACCCACTCCAAC
GAGAAACCCTACATCTGCAAGATCCCAGGCTGCACCAAGAGATACACAGACCCCAGCTCT
CTCCGGAAGCATGTGAAAACGGTCCACGGCCCAGATGCCCACGTCACCAAGAAGCAGCGC
AATGACGTGCACCTCCGGACACCGCTGCTCAAAGAGAATGGGGACAGTGAGGCCGGCACG
GAGCCTGGCGGCCCAGAGAGCACCGAGGCCAGCAGCACCAGTCAGGCCGTGGAGGACTGC
CTGCACGTCAGAGCCATCAAGACCGAGAGCTCCGGGCTGTGTCAGTCCAGCCCCGGAGCT
CAGTCGTCCTGCAGCAGTGAGCCCTCTCCTCTGGGCAGTGCCCCCAACAATGACAGTGGC
GTGGAGATGCCGGGGACGGGGCCCGGAAGCCTTGGAGATCTGACGGCACTTGATGACACA
CCCCCAGGGGCCGACACCTCAGCCCTTGCTGCCCCTTCCGCTGGTGGCCTCCAGCTGCGC
AAACACATGACCACCATGCACCGGTTCGAGCAGCTCAAGAAGGAGAAGCTCAAGTCACTC
AAGGATTCCTGCTCATGGGCCGGGCCGACTCCACACACGCGGAACACCAAGCTGCCTCCC
CTCCCGGGAAGTGGCTCCATCCTGGAAAACTTCAGTGGAAGTGGGGGCGGAGGGCCCGCG
GGGCTGCTGCCTAACCCGCGGCTTTCGGAACTGTCCGCGAGTGAGGTGACCATGCTGAGC
CAGCTGCAAGAGCGACGCGACAGCTCCACCAGCACGGTCAGCTCGGCTTACACCGTGAGC
CGCCGCTCCTCCGGAATTTCCCCCTACTTCTCTAGCCGCCGCTCTAGTGAGGCCTCGCCC
CTGGGCGCCGGACGCCCGCATAACGCGAGCTCTGCTGACTCCTACGACCCCATTTCTACG
GACGCGTCGAGGCGCTCGAGCGAGGCCAGTCAGTGCAGCGGCGGCTCTGGGCTTCTCAAC
CTTACGCCGGCGCAACAGTACAGTCTGCGGGCCAAGTACGCGGCAGCCACTGGAGGCCCC
CCGCCCACTCCGCTTCCGGGACTGGAGCGAATGAGTCTGCGGACAAGGCTTGCGCTGCTG
GACGCTCCTGAGCGCACACTTCCCGCTGGATGCCCACGCCCATTAGGGCCTAGGCGTGGC
AGTGACGGGCCGACATATGGCCACGGCCACGCTGGGGCTGCTCCCGCTTTCCCTCATGAA
GCTCCAGGCGGCGGAGCCAGAAGGGCTAGTGACCCTGTGCGGCGGCCTGATGCCCTGTCC
CTTCCGCGGGTGCAGCGCTTCCACAGCACACACAACGTGAACCCCGGACCGCTGCCTCCC
TGTGCCGATAGGCGAGGCCTCCGACTGCAAAGCCACCCGAGCACAGACGGCGGCCTTGCT
CGAGGCGCCTATTCTCCCAGGCCTCCTAGCATCAGTGAGAACGTGGCTATGGAAGCCGTG
GCGGCAGGAGTGGACGGCGCGGGGCCCGAGGCCGACCTTGGGCTGCCGGAGGATGACCTT
GTGCTTCCAGACGACGTGGTGCAGTACATCAAGGCGCACGCCAGTGGCGCTCTGGACGAG
GGCACCGGCCAGGTGTATCCCACGGAAAGCACTGGCTTCTCTGACAACCCCAGACTACCC
AGCCCGGGGCTGCACGGCCAGCGAAGGATGGTGGCTGCGGACTCCAACGTGGGACCCTCC
GCCCCTATGCTGGGAGGATGCCAGTTAGGCTTTGGGGCGCCCTCCAGCCTGAACAAAAAT
AACATGCCTGTGCAGTGGAATGAGGTGAGCTCCGGCACCGTAGACGCCCTGGCCAGCCAG
GTGAAGCCTCCACCCTTTCCTCAGGGCAACCTGGCGGTGGTGCAGCAGAAGCCTGCCTTT
GGCCAGTACCCGGGCTACAGTCCGCAAGGCCTACAGGCTAGCCCTGGGGGCCTTGACAGC
ACGCAGCCACATCTGCAGCCCCGCAGCGGAGCCCCCTCTCAGGGCATCCCCAGGGTAAAC
TACATGCAGCAGCTGCGACAGCCAGTGGCAGGCAGCCAGTGTCCTGGCATGACTACCACT
ATGAGCCCCCATGCCTGCTATGGCCAAGTCCACCCCCAGCTGAGCCCCAGCACCATCAGT
GGGGCCCTCAACCAGTTCCCCCAATCCTGCAGCAACATGCCAGCCAAGCCAGGGCATCTG
GGGCACCCTCAGCAGACAGAAGTGGCACCTGACCCCACCACGATGGGCAATCGCCACAGG
GAACTTGGGGTCCCCGATTCAGCCCTGGCTGGAGTGCCACCACCTCACCCAGTCCAGAGC
TACCCACAGCAGAGCCATCACCTGGCAGCCTCCATGAGCCAGGAGGGCTACCACCAGGTC
CCCAGCCTTCTGCCTGCCCGCCAGCCTGGCTTCATGGAGCCCCAAACAGGCCCGATGGGG
GTGGCTACAGCAGGCTTTGGCCTAGTGCAGCCCCGGCCTCCCTTAGAGCCCAGCCCCACT
GGACGCCACCGTGGGGTACGTGCTGTGCAGCAGCAGCTGGCCTACGCTAGGGCTACAGGC
CATGCCATGGCTGCCATGCCGTCCAGTCAGGAAACAGCAGAGGCTGTTCCCAAGGGAGCG
ATGGGAAACATGGGGTCGGTGCCTCCCCAGCCGCCTCCGCAGGACGCCGGTGGGGCCCCG
GACCACAGCATGCTCTACTACTACGGCCAGATCCACATGTACGAACAGGATGGAGGCCTG
GAGAACCTCGGGAGCTGCCAGGTCATGCGGTCCCAGCCACCACAGCCACAGGCCTGTCAG
GACAGCATCCAGCCCCAGCCCTTGCCCTCACCAGGGGTCAACCAGGTGTCCAGCACTGTG
GACTCCCAGCTCCTCGAGGCCCCCCAGATTGACTTCGATGCCATCATGGATGATGGCGAT
CACTCGAGTTTGTTCTCGGGTGCTCTGAGCCCCAGCCTCCTCCACAGCCTCTCCCAGAAC
TCCTCCCGGCTCACCACCCCCCGAAACTCCTTGACCCTGCCCTCCATCCCCGCAGGCATC
AGCAACATGGCTGTCGGGGACATGAGCTCCATGCTCACCAGCCTCGCCGAGGAGAGCAAG
TTCCTGAACATGATGACC
Protein sequence predicted based on nucleotide sequence
METSASATASEKQEAKSGILEAAGFPDPGKKASPLVVAAAAAAAVAAQGVPQHLLPPFHA PLPIDMRHQEGRYHYEPHSVHGVHGPPALSGSPVISDISLIRLSPHPAGPGESPFNAPHP YVNPHMEHYLRSVHSSPTLSMISAARGLSPADVAQEHLKERGLFGLPAPGTTPSDYYHQM TLVAGHPAPYGDLLMQSGGAASAPHLHDYLNPVDVSRFSSPRVTPRLSRKRALSISPLSD ASLDLQRMIRTSPNSLVAYINNSRSSSAASGSYGHLSAGALSPAFTFPHPINPVAYQQIL SQQRGLGSAFGHTPPLIQPSPTFLAQQPMALTSINATPTQLSSSSNCLSDTNQNKQSSES AVSSTVNPVAIHKRSKVKTEPEGLRPASPLALTQGQVSGHGSCGCALPLSQEQLADLKED LDRDDCKQEAEVVIYETNCHWEDCTKEYDTQEQLVHHINNEHIHGEKKEFVCRWQACTRE QKPFKAQYMLVVHMRRHTGEKPHKCTFEGCSKAYSRLENLKTHLRSHTGEKPYVCEHEGC NKAFSNASDRAKHQNRTHSNEKPYICKIPGCTKRYTDPSSLRKHVKTVHGPDAHVTKKQR NDVHLRTPLLKENGDSEAGTEPGGPESTEASSTSQAVEDCLHVRAIKTESSGLCQSSPGA QSSCSSEPSPLGSAPNNDSGVEMPGTGPGSLGDLTALDDTPPGADTSALAAPSAGGLQLR KHMTTMHRFEQLKKEKLKSLKDSCSWAGPTPHTRNTKLPPLPGSGSILENFSGSGGGGPA GLLPNPRLSELSASEVTMLSQLQERRDSSTSTVSSAYTVSRRSSGISPYFSSRRSSEASP LGAGRPHNASSADSYDPISTDASRRSSEASQCSGGSGLLNLTPAQQYSLRAKYAAATGGP PPTPLPGLERMSLRTRLALLDAPERTLPAGCPRPLGPRRGSDGPTYGHGHAGAAPAFPHE APGGGARRASDPVRRPDALSLPRVQRFHSTHNVNPGPLPPCADRRGLRLQSHPSTDGGLA RGAYSPRPPSISENVAMEAVAAGVDGAGPEADLGLPEDDLVLPDDVVQYIKAHASGALDE GTGQVYPTESTGFSDNPRLPSPGLHGQRRMVAADSNVGPSAPMLGGCQLGFGAPSSLNKN NMPVQWNEVSSGTVDALASQVKPPPFPQGNLAVVQQKPAFGQYPGYSPQGLQASPGGLDS TQPHLQPRSGAPSQGIPRVNYMQQLRQPVAGSQCPGMTTTMSPHACYGQVHPQLSPSTIS GALNQFPQSCSNMPAKPGHLGHPQQTEVAPDPTTMGNRHRELGVPDSALAGVPPPHPVQS YPQQSHHLAASMSQEGYHQVPSLLPARQPGFMEPQTGPMGVATAGFGLVQPRPPLEPSPT GRHRGVRAVQQQLAYARATGHAMAAMPSSQETAEAVPKGAMGNMGSVPPQPPPQDAGGAP DHSMLYYYGQIHMYEQDGGLENLGSCQVMRSQPPQPQACQDSIQPQPLPSPGVNQVSSTV DSQLLEAPQIDFDAIMDDGDHSSLFSGALSPSLLHSLSQNSSRLTTPRNSLTLPSIPAGI SNMAVGDMSSMLTSLAEESKFLNMMT
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pGW_NcoI-Eco109I
Synonyms:
pGW-NcoEco
Description:
In-fusion cloning vector with NcoI (5'att) and Eco109I (3'att) sites inserted to remove death cassette; kanamycin resistance in bacteria; recombinational cloning
Comments:
NcoI (5'att) and Eco109I (3'att) sites inserted to remove death cassette. Made for use with In-Fusion cloning
Size (bp):
4008
Parent Vector:
None
Properties:
''
Author Name:
Joshua LaBaer
Andreas Rolf
Andreas Rolf
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 bacterial origin
3332
4005
death cassette
ccdB
ccdB death cassette - removed in plasmids containing an insert
1779
2084
primer
M13 forward
M13 forward sequencing primer
536
553
primer
M13 reverse
M13 reverse sequencing primer
2269
2289
recombination site
attL1
attL1 recombinational cloning site
644
668
recombination site
attL2
attL2 recombinational cloning site
2133
2157
selectable marker
KanR
kanamycin resistance
2402
3208
selectable marker
CmR
Chloramphenicol resistance - removed in plasmids containing an insert
778
1434
