Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
None
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00744596
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 3789nts
Open reading frame: 1 to 3789
ATGCCCGCCCGTATCGGCTACTACGAGATCGACCGCACCATCGGCAAGGGCAACTTCGCG
GTGGTCAAGCGGGCCACGCACCTCGTCACCAAGGCCAAGGTTGCTATCAAGATCATAGAT
AAGACCCAGCTGGATGAAGAAAACTTGAAGAAGATTTTCCGGGAAGTTCAAATTATGAAG
ATGCTTTGCCACCCCCATATCATCAGGCTCTACCAGGTTATGGAGACAGAACGGATGATT
TATCTGGTGACAGAATATGCTAGTGGAGGGGAAATATTTGACCACCTGGTGGCCCATGGT
AGAATGGCAGAAAAGGAGGCACGTCGGAAGTTCAAACAGATCGTCACAGCTGTCTATTTT
TGTCACTGTCGGAACATTGTTCATCGTGATTTAAAAGCTGAAAATTTACTTCTGGATGCC
AATCTGAATATCAAAATAGCAGATTTTGGTTTCAGTAACCTCTTCACTCCTGGGCAGCTG
CTGAAGACCTGGTGTGGCAGCCCTCCCTATGCTGCACCTGAACTCTTTGAAGGAAAAGAA
TATGATGGGCCCAAAGTGGACATCTGGAGCCTTGGAGTTGTCCTCTACGTGCTTGTGTGC
GGTGCCCTGCCATTTGATGGAAGCACACTGCAGAATCTGCGGGCCCGCGTGCTGAGTGGA
AAGTTCCGCATCCCATTTTTTATGTCCACAGAATGTGAGCATTTGATCCGCCATATGTTG
GTGTTAGATCCCAATAAGCGCCTCTCCATGGAGCAGATCTGCAAGCACAAGTGGATGAAG
CTAGGGGACGCCGATCCCAACTTTGACAGGTTAATAGCTGAATGCCAACAACTAAAGGAA
GAAAGACAGGTGGACCCCCTGAATGAGGATGTCCTCTTGGCCATGGAGGACATGGGACTG
GACAAAGAACAGACACTGCAGTCATTAAGATCAGATGCCTATGATCACTATAGTGCAATC
TACAGCCTGCTGTGTGATCGACATAAGAGACATAAAACCCTGCGTCTCGGAGCACTTCCT
AGCATGCCCCGAGCCCTGGCCTTTCAAGCACCAGTCAATATCCAGGCGGAGCAGGCAGGT
ACTGCTATGAACATCAGCGTTCCCCAGGTGCAGCTGATCAACCCAGAGAACCAAATTGTG
GAGCCGGATGGGACACTGAATTTGGACAGTGATGAGGGTGAAGAGCCTTCCCCTGAAGCA
TTGGTGCGCTATTTGTCAATGAGGAGGCACACAGTGGGTGTGGCTGACCCACGCACGGAA
GTTATGGAAGATCTGCAGAAGCTCCTACCTGGCTTTCCTGGAGTCAACCCCCAGGCTCCA
TTCCTGCAGGTGGCCCCTAATGTGAACTTCATGCACAACCTGTTGCCTATGCAAAACTTG
CAACCAACCGGGCAACTTGAGTACAAGGAGCAGTCTCTCCTACAGCCGCCCACGCTACAG
CTGTTGAATGGAATGGGCCCCCTTGGCCGGAGGGCATCAGATGGAGGAGCCAACATCCAA
CTGCATGCCCAGCAGCTGCTGAAGCGCCCACGGGGACCCTCTCCGCTTGTCACCATGACA
CCAGCAGTGCCAGCAGTTACCCCTGTGGACGAGGAGAGCTCAGACGGGGAGCCAGACCAG
GAAGCTGTGCAGAGCTCTACCTACAAGGACTCCAACACTCTGCACCTCCCTACGGAGCGT
TTCTCCCCTGTGCGCCGGTTCTCAGATGGGGCTGCGAGCATCCAGGCCTTCAAAGCTCAC
CTGGAAAAAATGGGCAACAACAGCAGCATCAAACAGCTGCAGCAGGAGTGTGAGCAGCTG
CAGAAGATGTACGGGGGGCAGATTGATGAAAGAACCCTGGAGAAGACCCAGCAGCAGCAT
ATGTTATACCAGCAGGAGCAGCACCATCAAATTCTCCAGCAACAAATTCAAGACTCTATC
TGTCCTCCTCAGCCATCTCCACCTCTTCAGGCTGCATGTGAAAATCAGCCAGCCCTCCTT
ACCCATCAGCTCCAGAGGTTAAGGATTCAGCCTTCAAGCCCACCCCCCAACCACCCCAAC
AACCATCTCTTCAGGCAGCCCAGTAATAGTCCTCCCCCCATGAGCAGTGCCATGATCCAG
CCTCACGGGGCTGCATCTTCTTCCCAGTTTCAAGGCTTACCTTCCCGCAGTGCAATCTTT
CAGCAGCAACCTGAGAACTGTTCCTCTCCTCCCAACGTGGCACTAACCTGCTTGGGTATG
CAGCAGCCTGCTCAGTCACAGCAGGTCACCATCCAAGTCCAAGAGCCTGTTGACATGCTC
AGCAACATGCCAGGCACAGCTGCAGGCTCCAGTGGGCGCGGCATCTCCATCAGCCCCAGT
GCTGGTCAGATGCAGATGCAGCACCGTACCAACCTGATGGCCACCCTCAGCTATGGGCAC
CGTCCCTTGTCCAAGCAGCTGAGTGCTGACAGTGCAGAGGCTCACAGCTTGAACGTGAAT
CGGTTCTCCCCTGCTAACTACGACCAGGCGCATTTACACCCCCATCTGTTTTCGGACCAG
TCCCGGGGTTCCCCCAGCAGCTACAGCCCTTCAACAGGAGTGGGGTTCTCTCCAACCCAA
GCCCTGAAAGTCCCTCCACTTGACCAATTCCCCACCTTCCCTCCCAGTGCACATCAGCAG
CCGCCACACTATACCACGTCGGCACTACAGCAGGCCCTGCTGTCTCCCACGCCGCCAGAC
TATACAAGACACCAGCAGGTACCCCACATCCTTCAAGGACTGCTTTCTCCCCGGCATTCG
CTCACCGGCCACTCGGACATCCGGCTGCCCCCAACAGAGTTTGCACAGCTCATTAAAAGG
CAGCAGCAACAACGGCAGCAGCAGCAGCAACAGCAGCAACAGCAAGAATACCAGGAACTG
TTCAGGCACATGAACCAAGGGGATGCGGGGAGTCTGGCTCCCAGCCTTGGGGGACAGAGC
ATGACAGAGCGCCAGGCTTTATCTTATCAAAATGCTGACTCTTATCACCATCACACCAGC
CCCCAGCATCTGCTACAAATCAGGGCACAAGAATGTGTCTCACAGGCTTCCTCACCCACC
CCGCCCCACGGGTATGCTCACCAGCCGGCACTGATGCATTCAGAGAGCATGGAGGAGGAC
TGCTCGTGTGAGGGGGCCAAGGATGGCTTCCAAGACAGTAAGAGTTCAAGTACATTGACC
AAAGGTTGCCATGACAGCCCTCTGCTCTTGAGTACCGGTGGACCTGGGGACCCTGAATCT
TTGCTAGGAACTGTGAGTCATGCCCAAGAATTGGGGATACATCCCTATGGTCATCAGCCA
ACTGCTGCATTCAGTAAAAATAAGGTGCCCAGCAGAGAGCCTGTCATAGGGAACTGCATG
GATAGAAGTTCTCCAGGACAAGCAGTGGAGCTGCCGGATCACAATGGGCTCGGGTACCCA
GCACGCCCCTCCGTCCATGAGCACCACAGGCCCCGGGCCCTCCAGAGACACCACACGATC
CAGAACAGCGACGATGCTTATGTACAGCTGGATAACTTGCCAGGAATGAGTCTCGTGGCT
GGGAAAGCACTTAGCTCTGCCCGGATGTCGGATGCAGTTCTCAGTCAGTCTTCGCTCATG
GGCAGCCAGCAGTTTCAGGATGGGGAAAATGAGGAATGTGGGGCAAGCCTGGGAGGTCAT
GAGCACCCAGACCTGAGTGATGGCAGCCAGCATTTAAACTCCTCTTGCTATCCATCTACG
TGTATTACAGACATTCTGCTCAGCTACAAGCACCCCGAAGTCTCCTTCAGCATGGAGCAG
GCAGGCGTG
Protein sequence predicted based on nucleotide sequence
MPARIGYYEIDRTIGKGNFAVVKRATHLVTKAKVAIKIIDKTQLDEENLKKIFREVQIMK MLCHPHIIRLYQVMETERMIYLVTEYASGGEIFDHLVAHGRMAEKEARRKFKQIVTAVYF CHCRNIVHRDLKAENLLLDANLNIKIADFGFSNLFTPGQLLKTWCGSPPYAAPELFEGKE YDGPKVDIWSLGVVLYVLVCGALPFDGSTLQNLRARVLSGKFRIPFFMSTECEHLIRHML VLDPNKRLSMEQICKHKWMKLGDADPNFDRLIAECQQLKEERQVDPLNEDVLLAMEDMGL DKEQTLQSLRSDAYDHYSAIYSLLCDRHKRHKTLRLGALPSMPRALAFQAPVNIQAEQAG TAMNISVPQVQLINPENQIVEPDGTLNLDSDEGEEPSPEALVRYLSMRRHTVGVADPRTE VMEDLQKLLPGFPGVNPQAPFLQVAPNVNFMHNLLPMQNLQPTGQLEYKEQSLLQPPTLQ LLNGMGPLGRRASDGGANIQLHAQQLLKRPRGPSPLVTMTPAVPAVTPVDEESSDGEPDQ EAVQSSTYKDSNTLHLPTERFSPVRRFSDGAASIQAFKAHLEKMGNNSSIKQLQQECEQL QKMYGGQIDERTLEKTQQQHMLYQQEQHHQILQQQIQDSICPPQPSPPLQAACENQPALL THQLQRLRIQPSSPPPNHPNNHLFRQPSNSPPPMSSAMIQPHGAASSSQFQGLPSRSAIF QQQPENCSSPPNVALTCLGMQQPAQSQQVTIQVQEPVDMLSNMPGTAAGSSGRGISISPS AGQMQMQHRTNLMATLSYGHRPLSKQLSADSAEAHSLNVNRFSPANYDQAHLHPHLFSDQ SRGSPSSYSPSTGVGFSPTQALKVPPLDQFPTFPPSAHQQPPHYTTSALQQALLSPTPPD YTRHQQVPHILQGLLSPRHSLTGHSDIRLPPTEFAQLIKRQQQQRQQQQQQQQQQEYQEL FRHMNQGDAGSLAPSLGGQSMTERQALSYQNADSYHHHTSPQHLLQIRAQECVSQASSPT PPHGYAHQPALMHSESMEEDCSCEGAKDGFQDSKSSSTLTKGCHDSPLLLSTGGPGDPES LLGTVSHAQELGIHPYGHQPTAAFSKNKVPSREPVIGNCMDRSSPGQAVELPDHNGLGYP ARPSVHEHHRPRALQRHHTIQNSDDAYVQLDNLPGMSLVAGKALSSARMSDAVLSQSSLM GSQQFQDGENEECGASLGGHEHPDLSDGSQHLNSSCYPSTCITDILLSYKHPEVSFSMEQ AGV
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
