Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
Codon optimized
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
None
Description:
None
Comments:
Gene synthesis by Gen9. Codon optimized
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00744615
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 4158nts
Open reading frame: 1 to 4158
ATGCCTGAGGGAGCCCAAGGACTGAGCCTCTCCAAACCTAGCCCAAGCCTCGGGTGTGGC
CGAAGAGGTGAAGTCTGTGACTGTGGCACCGTGTGTGAGACTCGGACAGCTCCTGCAGCC
CCCACCATGGCCTCCCCCCGAGGTTCAGGGAGCTCCACATCGCTGAGCACAGTGGGCTCT
GAGGGGGATCCCGCCCCTGGGCCCACTCCAGCCTGCTCAGCCTCCAGGCCAGAGCCCCTT
CCAGGGCCTCCCATCCGCCTACATCTCTCTCCGGTGGGGATCCCAGGTTCAGCCAGACCT
TCAAGGCTGGAGCGTGTGGCTCGGGAGATCGTGGAGACAGAACGGGCCTATGTCAGGGAC
CTGCGCAGCATCGTGGAGGACTACCTGGGCCCTCTGCTGGACGGCGGGGTCCTGGGGCTG
AGCGTGGAGCAGGTCGGCACGCTGTTTGCCAACATTGAGGACATCTACGAGTTCAGCAGC
GAGCTCCTGGAGGACTTGGAGAACAGCAGCAGCGCCGGGGGTATTGCCGAGTGCTTCGTG
CAGAGGAGCGAGGATTTTGACATCTACACATTGTACTGCATGAACTACCCGAGCTCCCTG
GCCCTGCTTCGGGAGCTGTCGTTGTCTCCGCCAGCAGCCCTGTGGCTGCAGGAGCGCCAG
GCCCAGCTTCGCCACTCGCTGCCCCTGCAGAGCTTCCTGCTGAAACCTGTCCAGCGCATT
CTCAAGTACCATCTGCTGCTGCAGGAACTAGGGAAGCACTGGGCGGAGGGACCAGGCACT
GGGGGTCGCGAGATGGTGGAGGAAGCTATTGTGTCTATGACAGCGGTTGCTTGGTACATC
AACGACATGAAGCGCAAGCAGGAGCATGCAGCGCGCCTCCAGGAAGTGCAGCGGCGGCTG
GGTGGATGGACCGGACCAGAGCTCAGTGCTTTTGGGGAACTGGTGTTGGAGGGCGCGTTC
CGAGGAGGCGGAGGGGGCGGCCCCCGGCTACGAGGGGGTGAGAGGCTGCTCTTCCTGTTC
TCTCGGATGCTGCTGGTGGCCAAGCGCAGGGGGCTGGAGTACACCTACAAAGGCCACATC
TTCTGCTGCAACCTGAGCGTGAGCGAGAGTCCCCGGGACCCCCTAGGGTTCAAGGTGTCT
GATCTGACCATTCCCAAGCACAGACACCTCCTCCAGGCCAAGAACCAAGAAGAGAAGAGG
CTGTGGATTCACTGTCTCCAGCGCCTCTTCTTTGAGAACCACCCTGCCTCCATCCCTGCC
AAGGCAAAGCAAGTTCTCCTTGAAAACAGTCTGCATTGTGCCCCCAAAAGTAAGCCTGTC
CTAGAGCCCCTGACACCCCCACTTGGCTCTCCTCGACCTCGAGATGCTAGAAGTTTTACC
CCTGGGAGAAGGAACACCGCTCCATCTCCTGGGCCCTCTGTGATTCGCCGAGGCCGGAGG
CAGTCTGAGCCGGTGAAGGACCCTTATGTCATGTTCCCACAGAACGCTAAGCCTGGATTC
AAGCACGCTGGCAGCGAAGGGGAACTGTACCCTCCAGAATCCCAGCCACCAGTTTCAGGC
TCTGCACCCCCTGAGGACCTGGAGGATGCTGGACCCCCAACACTGGACCCCTCTGGGACC
TCAATCACTGAAGAAATCCTGGAACTGCTGAATCAGCGAGGCCTTCGAGATCCAGGGCCG
TCCACCCATGACATTCCCAAGTTCCCCGGAGACTCCCAGGTGCCTGGCGACAGCGAAACC
CTCACATTCCAAGCCCTGCCCAGCCGGGACTCTTCAGAAGAGGAGGAGGAGGAAGAGGAA
GGGCTGGAGATGGATGAACGGGGGCCTTCCCCACTCCACGTCCTGGAAGGGCTGGAAAGT
TCTATTGCAGCTGAAATGCCCAGCATTCCCTGCCTTACCAAAATTCCTGACGTGCCCAAC
CTTCCTGAAATTCCCAGTCGGTGTGAAATTCCCGAAGGTTCTCGCCTTCCTAGTCTCTCT
GACATTTCCGATGTTTTTGAGATGCCCTGCCTTCCAGCCATACCTAGTGTCCCCAACACC
CCCAGTCTGTCTAGCACTCCCACCCTCTCCTGTGACTCCTGGCTCCAAGGGCCTCTGCAG
GAACCAGCTGAGGCTCCAGCCACCAGGAGAGAATTGTTTTCTGGGAGCAATCCTGGGAAA
CTGGGAGAGCCGCCTTCAGGAGGCAAGGCAGGGCCAGAGGAGGATGAAGAAGGGGTATCA
TTCACAGACTTCCAGCCCCAGGATGTCACCCAACATCAGGGATTCCCAGATGAGCTGGCA
TTCCGCTCTTGCTCAGAAATCCGGAGCGCCTGGCAGGCATTGGAACAGGGACAGCTGGCC
CGGCCAGGCTTCCCAGAGCCACTGCTCATCCTGGAGGATTCGGATCTGGGTGGAGACAGC
GGGAGCGGGAAGGCAGGAGCCCCGAGTTCAGAAAGGACGGCGTCCAGAGTGCGAGAGCTG
GCCCGGCTTTACAGCGAGCGGATCCAGCAGATGCAGAGGGCGGAGACTCGGGCCTCAGCC
AATGCCCCGCGACGCCGGCCTCGGGTTCTTGCCCAACCCCAGCCATCCCCCTGTCTTCCC
CAGGAGCAGGCAGAGCCAGGGCTCCTGCCTGCCTTTGGACACGTGCTGGTATGTGAGCTG
GCCTTCCCACTGACATGTGCCCAGGAGTCTGTCCCCCTGGGTCCTGCTGTCTGGGTTCAA
GCTGCCATACCTTTGTCAAAGCAGGGAGGCAGCCCCGATGGCCAGGGTCTACATGTTTCC
AATTTGCCTAAGCAAGACCTTCCGGGCATCCACGTTTCAGCTGCTACCCTTTTGCCTGAG
CAAGGAGGTTCCCGGCATGTCCAGGCTCCAGCCGCCACACCTTTGCCTAAGCAAGAAGGC
CCCCTGCACCTCCAGGTGCCGGCTCTTACAACCTTCTCTGATCAAGGCCACCCAGAAATC
CAAGTTCCAGCCACCACTCCTTTGCCTGAGCATAGAAGTCACATGGTTATACCAGCTCCA
TCCACCGCCTTTTGTCCTGAGCAGGGACACTGTGCGGACATCCACGTTCCTACCACTCCA
GCTTTGCCTAAGGAGATTTGTTCTGATTTCACAGTTTCAGTCACCACCCCTGTGCCCAAG
CAAGAAGGTCACCTAGACAGCGAGAGCCCAACCAATATCCCACTGACCAAGCAAGGAGGT
TCCAGGGATGTTCAGGGCCCAGACCCTGTCTGCAGTCAACCCATCCAGCCCTTGTCCTGG
CATGGAAGCAGCCTGGATCCCCAGGGCCCAGGCGACACCCTACCACCCTTGCCATGTCAC
CTCCCAGACCTTCAGATTCCAGGTACCTCACCTTTGCCTGCACATGGAAGCCACCTCGAC
CATCGGATCCCAGCCAACGCCCCACTCTCTTTGTCACAGGAGTTGCCAGACACTCAGGTT
CCAGCTACCACACCTTTGCCCCTGCCACAAGTCCTCACAGACATCTGGGTCCAAGCCCTC
CCAACTTCACCCAAGCAGGGAAGCCTCCCAGACATCCAGGGTCCAGCGGCTGCACCTCCA
CTTCCGGAGCCAAGCCTTACAGATACACAGGTCCAAAAACTCACACCCTCGTTGGAGCAG
AAGAGCCTCATAGATGCCCATGTTCCAGCTGCCACACCTTTACCTGAGAGAGGAGGCTCT
CTAGACATTCAGGGCCTCTCACCCACCCCAGTTCAGACCACCATGGTTTTGTCCAAACCA
GGAGGCTCCTTAGCCTCTCACGTTGCCAGGTTGGAGTCTTCAGACTTGACGCCACCTCAT
AGTCCCCCACCTTCCAGCCGTCAGCTGCTGGGACCCAATGCAGCTGCCCTCTCTAGATAC
CTGGCAGCCTCATATATCAGCCAGAGTCTGGCTCGGCGGCAGGGACCTGGAGGAGGGGCC
CCCGCAGCCTCTCGGGGCTCTTGGTCTTCTGCTCCCACATCAAGGGCATCTTCGCCGCCT
CCCCAGCCCCAGCCACCACCTCCTCCAGCCAGGCGGCTCAGCTATGCCACGACGGTTAAC
ATCCATGTGGGCGGGGGAGGGCGGTTGCGGCCAGCCAAGGCCCAGGTCCGGTTGAACCAC
CCTGCTCTCTTGGCCTCCACACAGGAATCTATGGGCCTTCACAGAGCCCAGGGCGCTCCT
GATGCCCCCTTCCACATG
Protein sequence predicted based on nucleotide sequence
MPEGAQGLSLSKPSPSLGCGRRGEVCDCGTVCETRTAPAAPTMASPRGSGSSTSLSTVGS EGDPAPGPTPACSASRPEPLPGPPIRLHLSPVGIPGSARPSRLERVAREIVETERAYVRD LRSIVEDYLGPLLDGGVLGLSVEQVGTLFANIEDIYEFSSELLEDLENSSSAGGIAECFV QRSEDFDIYTLYCMNYPSSLALLRELSLSPPAALWLQERQAQLRHSLPLQSFLLKPVQRI LKYHLLLQELGKHWAEGPGTGGREMVEEAIVSMTAVAWYINDMKRKQEHAARLQEVQRRL GGWTGPELSAFGELVLEGAFRGGGGGGPRLRGGERLLFLFSRMLLVAKRRGLEYTYKGHI FCCNLSVSESPRDPLGFKVSDLTIPKHRHLLQAKNQEEKRLWIHCLQRLFFENHPASIPA KAKQVLLENSLHCAPKSKPVLEPLTPPLGSPRPRDARSFTPGRRNTAPSPGPSVIRRGRR QSEPVKDPYVMFPQNAKPGFKHAGSEGELYPPESQPPVSGSAPPEDLEDAGPPTLDPSGT SITEEILELLNQRGLRDPGPSTHDIPKFPGDSQVPGDSETLTFQALPSRDSSEEEEEEEE GLEMDERGPSPLHVLEGLESSIAAEMPSIPCLTKIPDVPNLPEIPSRCEIPEGSRLPSLS DISDVFEMPCLPAIPSVPNTPSLSSTPTLSCDSWLQGPLQEPAEAPATRRELFSGSNPGK LGEPPSGGKAGPEEDEEGVSFTDFQPQDVTQHQGFPDELAFRSCSEIRSAWQALEQGQLA RPGFPEPLLILEDSDLGGDSGSGKAGAPSSERTASRVRELARLYSERIQQMQRAETRASA NAPRRRPRVLAQPQPSPCLPQEQAEPGLLPAFGHVLVCELAFPLTCAQESVPLGPAVWVQ AAIPLSKQGGSPDGQGLHVSNLPKQDLPGIHVSAATLLPEQGGSRHVQAPAATPLPKQEG PLHLQVPALTTFSDQGHPEIQVPATTPLPEHRSHMVIPAPSTAFCPEQGHCADIHVPTTP ALPKEICSDFTVSVTTPVPKQEGHLDSESPTNIPLTKQGGSRDVQGPDPVCSQPIQPLSW HGSSLDPQGPGDTLPPLPCHLPDLQIPGTSPLPAHGSHLDHRIPANAPLSLSQELPDTQV PATTPLPLPQVLTDIWVQALPTSPKQGSLPDIQGPAAAPPLPEPSLTDTQVQKLTPSLEQ KSLIDAHVPAATPLPERGGSLDIQGLSPTPVQTTMVLSKPGGSLASHVARLESSDLTPPH SPPPSSRQLLGPNAAALSRYLAASYISQSLARRQGPGGGAPAASRGSWSSAPTSRASSPP PQPQPPPPPARRLSYATTVNIHVGGGGRLRPAKAQVRLNHPALLASTQESMGLHRAQGAP DAPFHM
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
