Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
Codon optimized
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
None
Description:
None
Comments:
Gene synthesis by Gen9. Codon optimized
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00744666
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 3180nts
Open reading frame: 1 to 3180
ATGGAACTTTGGGCTCCCCAGAGGCTGCCCCAGACACGAGGGAAGGCCACAGCACCCTCA
AAGGATCCAGACCGAGGGTTTCGGAGAGATGGACATCATCGGCCTGTCCCTCACTCTTGG
CACAATGGAGAGAGGTTTCACCAATGGCAAGACAACCGTGGGAGCCCCCAGCCACAGCAG
GAGCCCAGGGCAGACCATCAGCAGCAGCCCCATTATGCATCCAGGCCAGGGGACTGGCAT
CAGCCTGTGTCTGGAGTTGACTATTACGAAGGTGGTTATCGCAATCAGTTGTATTCAAGG
CCAGGTTATGAGAATTCATATCAGAGCTATCAGTCTCCCACAATGAGGGAGGAATATGCT
TATGGAAGTTATTACTATCATGGACACCCACAGTGGCTGCAGGAAGAAAGAGTGCCAAGG
CAACGGAGTCCTTATATCTGGCACGAAGATTACCGAGAGCAAAAGTACCTTGATGAACAT
CATTATGAAAACCAGCACAGTCCATTTGGAACAAATAGTGAGACTCACTTCCAATCTAAC
AGTAGGAACCCTTGTAAAGACAGCCCTGCTTCCAACTCTGGACAGGAGTGGCCGGGGGAG
CTGTTTCCAGGGAGCCTGCTTGCTGAGGCCCAGAAAAATAAGCCATCATTGGCTAGTGAG
TCCAACCTTCTCCAGCAGCGTGAGTCTGGTCTAAGCTCCAGCAGCTATGAGCTCAGTCAG
TACATCAGAGATGCCCCGGAGCGGGATGATCCCCCAGCTTCAGCAGCTTGGAGTCCAGTT
CAGGCTGATGTCTCCTCAGCTGGTCCCAAAGCACCCATGAAGTTCTACATCCCTCATGTT
CCTGTGAGTTTCGGGCCAGGAGGTCAGCTGGTGCATGTAGGTCCCAGCTCTCCCACTGAC
GGGCAAGCAGCCCTTGTTGAACTGCACAGCATGGAGGTTATTCTTAATGATTCCGAAGAG
CAAGAGGAGATGAGAAGTTTCTCAGGACCCTTGATTAGGGAAGATGTACATAAGGTGGAT
ATTATGACGTTTTGCCAGCAGAAAGCAGCTCAGAGCTGCAAATCTGAGACACTGGGGAGC
AGAGACTCAGCTCTACTGTGGCAGCTCTTGGTTCTCCTTTGTCGCCAGAATGGGTCCATG
GTGGGGTCTGACATCGCTGAGCTGCTAATGCAAGACTGCAAGAAGCTGGAGAAGTACAAG
CGGCAGCCCCCTGTGGCCAACCTCATCAACCTGACCGATGAGGACTGGCCAGTGCTGAGC
TCTGGGACCCCGAACCTGCTCACGGGAGAGATCCCCCCCAGTGTGGAGACACCGGCGCAG
ATCGTGGAGAAATTCACTAGGCTGCTCTACTATGGAAGGAAGAAGGAAGCCTTGGAGTGG
GCCATGAAGAACCACTTGTGGGGCCATGCTTTGTTCCTGTCCAGCAAGATGGACCCACAG
ACCTACAGCTGGGTCATGAGTGGCTTCACCAGCACGCTGGCGCTCAATGACCCACTGCAG
ACCCTCTTCCAGCTCATGTCGGGGAGGATTCCACAGGCAGCCACGTGTTGTGGAGAAAAG
CAGTGGGGAGACTGGAGGCCTCACTTGGCTGTGATTCTGTCGAATCAGGCTGGGGACCCA
GAGCTGTATCAGCGTGCGATTGTTGCCATTGGGGACACCCTGGCTGGGAAGGGGCTTGTG
GAGGCAGCTCACTTCTGCTATCTCATGGCTCACGTGCCCTTTGGCCACTACACCGTGAAG
ACAGACCATCTGGTCTTGCTGGGCAGCAGCCACAGTCAAGAGTTTTTGAAATTTGCAACA
ACTGAGGCAATCCAGAGGACGGAAATCTTCGAGTACTGTCAGATGCTGGGCCGCCCCAAA
TCCTTCATCCCTTCTTTCCAGGTGTATAAGCTCCTTTATGCTTCCCGTCTGGCAGATTAT
GGCCTCGTGTCCCAGGCTTTGCATTACTGCGAAGCCATTGGTGCAGCTGTCTTGAGCCAG
GGAGAGAGCAGTCACCCTGTGCTATTAGTGGAACTCATCAAGCTAGCAGAGAAACTGAAG
CTGTCAGATCCTCTGGTTTTAGAAAGACGCAGTGGAGACAGGGACCTGGAGCCAGATTGG
CTAGCGCAACTTCGGAGGCAGCTGGAGCAAAAGGTAGCAGGAGACATTGGGGATCCTCAT
CCTACTCGCTCAGATATTTCGGGAGCCGGAGGAACAACAACAGAAAACACTTTCTACCAG
GACTTTTCTGGATGTCAAGGCTACTCTGAAGCCCCTGGGTACCGCTCAGCTCTGTGGCTG
ACACCTGAGCAGACCTGCCTGCTCCAGCCCAGCCCACAGCAGCCCTTTCCCCTCCAGCCG
GGCTCCTACCCAGCAGGAGGGGGTGCAGGGCAGACAGGGACACCGAGGCCTTTTTACTCA
GTTCCTGAGACGCATCTACCAGGGACTGGCAGCAGCGTGGCAGTGACAGAGGCCACTGGA
GGAACAGTCTGGGAGGAAATGCTGCAGACACACCTGGGCCCTGGAGAGAACACAGTGTCT
CAAGAAACTTCCCAGCCTCCTGATGGCCAAGAGGTCATTTCCAAACCACAGACACCATTG
GCTGCTAGACCACGAAGTATTTCTGAGAGTTCCGCCAGTTCAGCCAAGGAGGATGAGAAG
GAGTCCTCTGATGAGGCTGATAAAAACTCTCCCCGAAATACTGCCCAGAGAGGCAAGCTC
GGAGATGGGAAGGAGCATACAAAGAGCTCAGGGTTTGGCTGGTTCAGCTGGTTTCGATCG
AAGCCCACCAAGAACGCATCCCCCGCTGGAGACGAGGACTCCTCAGACAGCCCTGACTCT
GAGGAGACGCCCAGAGCATCTTCTCCCCACCAGGCTGGCCTGGGCCTCTCACTGACACCT
TCCCCTGAGTCCCCACCTCTGCCGGATGTTAGTGCCTTCTCCAGGGGCAGAGGTGGGGGT
GAAGGCCGAGGATCCGCATCCAGCGGGGGAGCAGCTGCGGGCGCTGGGGTTGGAGGCTTG
TCTGGACCAGAGAGTGTTTCCTTTGAGCTCTGCTCCAACCCTGGTGTTCTTCTTCCTCCA
CCCGCCTTAAAAGGGGCTGTTCCTCTTTACAACCCATCTCAGGTGCCTCAGCTGCCCACG
GCCACTAGCCTGAATCGGCCAAATCGCCTAGCTCAGCGTCGCTATCCCACCCAGCCATGC
Protein sequence predicted based on nucleotide sequence
MELWAPQRLPQTRGKATAPSKDPDRGFRRDGHHRPVPHSWHNGERFHQWQDNRGSPQPQQ EPRADHQQQPHYASRPGDWHQPVSGVDYYEGGYRNQLYSRPGYENSYQSYQSPTMREEYA YGSYYYHGHPQWLQEERVPRQRSPYIWHEDYREQKYLDEHHYENQHSPFGTNSETHFQSN SRNPCKDSPASNSGQEWPGELFPGSLLAEAQKNKPSLASESNLLQQRESGLSSSSYELSQ YIRDAPERDDPPASAAWSPVQADVSSAGPKAPMKFYIPHVPVSFGPGGQLVHVGPSSPTD GQAALVELHSMEVILNDSEEQEEMRSFSGPLIREDVHKVDIMTFCQQKAAQSCKSETLGS RDSALLWQLLVLLCRQNGSMVGSDIAELLMQDCKKLEKYKRQPPVANLINLTDEDWPVLS SGTPNLLTGEIPPSVETPAQIVEKFTRLLYYGRKKEALEWAMKNHLWGHALFLSSKMDPQ TYSWVMSGFTSTLALNDPLQTLFQLMSGRIPQAATCCGEKQWGDWRPHLAVILSNQAGDP ELYQRAIVAIGDTLAGKGLVEAAHFCYLMAHVPFGHYTVKTDHLVLLGSSHSQEFLKFAT TEAIQRTEIFEYCQMLGRPKSFIPSFQVYKLLYASRLADYGLVSQALHYCEAIGAAVLSQ GESSHPVLLVELIKLAEKLKLSDPLVLERRSGDRDLEPDWLAQLRRQLEQKVAGDIGDPH PTRSDISGAGGTTTENTFYQDFSGCQGYSEAPGYRSALWLTPEQTCLLQPSPQQPFPLQP GSYPAGGGAGQTGTPRPFYSVPETHLPGTGSSVAVTEATGGTVWEEMLQTHLGPGENTVS QETSQPPDGQEVISKPQTPLAARPRSISESSASSAKEDEKESSDEADKNSPRNTAQRGKL GDGKEHTKSSGFGWFSWFRSKPTKNASPAGDEDSSDSPDSEETPRASSPHQAGLGLSLTP SPESPPLPDVSAFSRGRGGGEGRGSASSGGAAAGAGVGGLSGPESVSFELCSNPGVLLPP PALKGAVPLYNPSQVPQLPTATSLNRPNRLAQRRYPTQPC
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
