Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
Codon optimized
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pGW_NcoI-Eco109I
Format:
FUSION
Source:
None
Description:
None
Comments:
Gene synthesis by Gen9. Codon optimized
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00744713
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 4341nts
Open reading frame: 1 to 4341
ATGGCTCATTCAAAGACTAGAACCAATGATGGAAAAATTACATATCCGCCTGGGGTCAAG
GAAATATCAGATAAAATATCTAAAGAGGAGATGGTGAGACGGTTAAAGATGGTGGTGAAA
ACTTTTATGGATATGGACCAGGACTCTGAAGAAGAAAAGGAGCTTTATTTAAACCTAGCT
TTACATCTTGCTTCAGATTTTTTTCTCAAGCATCCTGATAAAGATGTTCGCTTACTGGTA
GCCTGCTGCCTTGCTGATATTTTCAGGATTTATGCTCCTGAAGCTCCTTACACATCCCCT
GACAAACTGAAGGATATATTTATGTTTATAACAAGGCAGTTGAAGGGGCTAGAGGATACC
AAGAGCCCACAATTCAATAGGTATTTTTATTTACTTGAGAACATTGCTTGGGTCAAGTCA
TACAACATATGCTTTGAGCTGGAAGATAGCAATGAAATTTTCACCCAGCTATACAGAACC
TTATTTTCAGTTATAAACAATGGCCACAATCAGAAAGTCCATATGCACATGGTAGACCTT
ATGAGCTCTATTATTTGTGAAGGTGATACAGTGTCTCAGGAGCTGTTGGATACGGTTTTA
GTAAATCTGGTACCTGCTCATAAGAATTTAAACAAGCAGGCTTATGATTTGGCAAAGGCT
TTACTGAAGAGGACAGCTCAAGCTATTGAGCCATATATTACCAATTTTTTTAATCAGGTT
CTGATGCTGGGGAAAACATCTATCAGCGATTTGTCAGAGCATGTCTTTGACTTAATTTTG
GAGCTGTACAATATTGATAGTCATTTGCTGCTCTCTGTTTTGCCCCAGCTTGAATTTAAA
TTGAAGAGCAATGATAATGAGGAGCGCCTACAAGTTGTTAAACTACTGGCAAAAATGTTT
GGGGCCAAGGATTCAGAATTGGCTTCTCAAAACAAGCCACTTTGGCAGTGCTACTTGGGC
AGGTTTAATGATATCCATGTACCAATCCGCCTCGAATGTGTGAAATTTGCTAGTCATTGT
CTCATGAACCATCCTGATTTAGCAAAAGATTTAACAGAGTATCTTAAAGTGAGGTCACAT
GACCCTGAGGAAGCTATTAGACATGATGTCATTGTGTCAATAGTTACAGCTGCTAAAAAG
GATATTCTTCTGGTCAATGATCACTTACTTAATTTTGTGAGAGAGAGAACACTGGACAAA
CGATGGAGAGTACGCAAAGAAGCCATGATGGGACTTGCCCAAATTTATAAGAAATATGCT
TTACAGTCAGCCGCTGGCAAAGATGCTGCCAAACAGATAGCATGGATCAAAGACAAATTG
CTACACATATATTATCAAAATAGTATTGATGATCGACTACTGGTTGAACGGATCTTTGCC
CAATACATGGTTCCCCACAATTTAGAAACTACAGAACGGATGAAATGCTTATATTACTTG
TATGCCACACTGGATCTCAATGCTGTGAAAGCATTGAATGAAATGTGGAAATGTCAAAAT
CTCCTCCGACATCAAGTAAAGGATTTGCTTGACTTGATTAAGCAACCCAAAACCGATGCC
AGTGTCAAGGCCATATTTTCAAAAGTGATGGTTATTACAAGGAATTTACCTGATCCTGGT
AAGGCTCAGGATTTCATGAAGAAATTCACACAGGTGTTGGAAGATGATGAGAAAATAAGA
AAGCAGTTAGAAGTACTTGTTAGTCCAACCTGCTCCTGCAAGCAGGCTGAAGGCTGTGTG
CGTGAAATAACTAAGAAGTTGGGCAACCCCAAACAGCCTACAAATCCTTTCCTGGAAATG
ATCAAGTTTCTCTTGGAGAGGATAGCACCTGTCCACATAGATACCGAATCTATCAGTGCT
CTTATTAAACAAGTGAACAAATCTATAGATGGAACAGCAGATGATGAAGATGAGGGTGTT
CCAACTGATCAAGCCATCAGAGCAGGTCTTGAACTGCTTAAGGTACTGTCATTTACACAT
CCCATCTCATTTCATTCCGCTGAAACATTTGAATCATTACTGGCTTGTCTGAAAATGGAT
GATGAAAAAGTAGCAGAAGCTGCACTGCAAATTTTCAAAAACACAGGCAGCAAAATTGAA
GAGGATTTTCCACACATCAGATCCGCCCTGCTTCCTGTTTTACATCACAAATCCAAAAAA
GGACCCCCCCGTCAAGCCAAATATGCCATTCATTGTATCCATGCGATATTTTCTAGTAAA
GAGACACAGTTTGCACAGATATTTGAGCCTCTGCATAAGAGCCTAGATCCAAGCAACCTG
GAACATCTCATAACACCATTGGTTACTATTGGTCATATTGCTCTCCTTGCACCTGATCAA
TTTGCTGCTCCTTTGAAATCTTTGGTAGCTACTTTCATTGTGAAAGATCTTCTCATGAAT
GATCGGCTTCCAGGGAAAAAGACAACTAAACTTTGGGTTCCAGATGAAGAAGTATCTCCT
GAGACAATGGTCAAAATTCAGGCTATTAAAATGATGGTTCGATGGCTCCTTGGAATGAAA
AATAATCACAGTAAATCCGGAACTTCTACCTTAAGATTGCTAACAACAATACTGCATAGT
GATGGAGACTTGACAGAACAGGGGAAAATTAGTAAACCAGATATGTCACGTCTGAGACTT
GCTGCTGGGAGTGCTATTGTGAAGCTGGCACAAGAACCCTGTTACCATGAAATCATCACA
TTAGAACAATATCAGCTATGTGCACTAGCTATCAACGATGAATGCTATCAAGTAAGACAA
GTGTTTGCCCAGAAACTTCACAAAGGCCTTTCCCGTTTACGGCTTCCACTGGAGTATATG
GCAATCTGTGCCCTTTGTGCAAAAGATCCTGTAAAGGAGAGAAGAGCTCATGCTAGGCAA
TGTTTGGTGAAAAATATAAATGTAAGGCGGGAGTATCTCAAGCAGCATGCAGCTGTTAGC
GAAAAATTATTGTCTCTTCTACCAGAGTATGTTGTTCCATATACAATTCACCTTTTGGCA
CATGACCCAGACTATGTCAAAGTACAGGATATTGAACAACTTAAAGATGTTAAAGAGTGT
CTGTGGTTTGTGCTGGAGATATTAATGGCCAAAAACGAAAATAACAGTCACGCTTTTATC
AGGAAGATGGTGGAAAATATTAAACAAACAAAAGATGCCCAAGGACCAGATGATGCAAAA
ATGAATGAGAAACTGTACACTGTGTGTGATGTTGCCATGAATATCATCATGTCAAAGAGT
ACTACATACAGTTTAGAATCTCCTAAAGACCCGGTACTACCAGCTCGTTTCTTCACTCAA
CCTGACAAGAATTTCAGTAACACCAAAAATTATCTCCCTCCTGAAATGAAATCATTTTTC
ACTCCTGGAAAACCTAAAACAACCAATGTGCTAGGAGCTGTTAACAAGCCACTTTCATCA
GCAGGCAAGCAATCTCAGACCAAATCATCACGAATGGAAACCGTAAGCAATGCAAGCAGC
AGCTCAAATCCAAGCTCTCCTGGAAGAATAAAGGGGAGGCTTGATAGCTCGGAAATGGAT
CACAGTGAAAATGAAGATTACACAATGTCTTCACCCTTGCCCGGGAAAAAAAGTGACAAG
AGGGACGACTCTGATCTTGTAAGGTCTGAATTGGAGAAGCCTAGGGGCAGGAAAAAAACG
CCTGTCACAGAACAGGAGGAGAAATTAGGTATGGATGACTTGACTAAGTTGGTACAGGAA
CAGAAACCTAAAGGCAGTCAGCGAAGCCGGAAAAGAGGCCATACGGCTTCAGAATCTGAT
GAACAGCAGTGGCCTGAGGAAAAGAGGCTCAAAGAAGATATATTAGAAAATGAAGATGAA
CAGAATAGTCCGCCAAAAAAGGGTAAAAGAGGACGACCACCAAAACCTCTTGGTGGAGGT
ACACCAAAAGAAGAGCCAACAATGAAAACTTCTAAAAAAGGAAGCAAAAAGAAATCTGGA
CCCCCAGCACCAGAGGAGGAGGAAGAAGAAGAAAGACAAAGCGGAAATACGGAACAGAAG
TCCAAAAGCAAACAGCACCGAGTGTCAAGGAGAGCCCAGCAGAGAGCAGAATCTCCTGAA
TCTAGTGCAATTGAATCCACACAGTCCACACCACAGAAAGGACGAGGACGACCCTCAAAA
ACGCCATCACCATCACAACCCAAAAAAAATGTCCGTGTAGGACGCTCCAAACAAGCAGCT
ACTAAGGAAAATGATTCAAGTGAAGAAGTAGATGTGTTTCAGGGTAGCTCTCCTGTCGAT
GATATTCCACAGGAAGAAACAGAGGAGGAGGAAGTTTCCACAGTAAATGTACGGCGGCGA
AGTGCTAAAAGGGAACGGCGA
Protein sequence predicted based on nucleotide sequence
MAHSKTRTNDGKITYPPGVKEISDKISKEEMVRRLKMVVKTFMDMDQDSEEEKELYLNLA LHLASDFFLKHPDKDVRLLVACCLADIFRIYAPEAPYTSPDKLKDIFMFITRQLKGLEDT KSPQFNRYFYLLENIAWVKSYNICFELEDSNEIFTQLYRTLFSVINNGHNQKVHMHMVDL MSSIICEGDTVSQELLDTVLVNLVPAHKNLNKQAYDLAKALLKRTAQAIEPYITNFFNQV LMLGKTSISDLSEHVFDLILELYNIDSHLLLSVLPQLEFKLKSNDNEERLQVVKLLAKMF GAKDSELASQNKPLWQCYLGRFNDIHVPIRLECVKFASHCLMNHPDLAKDLTEYLKVRSH DPEEAIRHDVIVSIVTAAKKDILLVNDHLLNFVRERTLDKRWRVRKEAMMGLAQIYKKYA LQSAAGKDAAKQIAWIKDKLLHIYYQNSIDDRLLVERIFAQYMVPHNLETTERMKCLYYL YATLDLNAVKALNEMWKCQNLLRHQVKDLLDLIKQPKTDASVKAIFSKVMVITRNLPDPG KAQDFMKKFTQVLEDDEKIRKQLEVLVSPTCSCKQAEGCVREITKKLGNPKQPTNPFLEM IKFLLERIAPVHIDTESISALIKQVNKSIDGTADDEDEGVPTDQAIRAGLELLKVLSFTH PISFHSAETFESLLACLKMDDEKVAEAALQIFKNTGSKIEEDFPHIRSALLPVLHHKSKK GPPRQAKYAIHCIHAIFSSKETQFAQIFEPLHKSLDPSNLEHLITPLVTIGHIALLAPDQ FAAPLKSLVATFIVKDLLMNDRLPGKKTTKLWVPDEEVSPETMVKIQAIKMMVRWLLGMK NNHSKSGTSTLRLLTTILHSDGDLTEQGKISKPDMSRLRLAAGSAIVKLAQEPCYHEIIT LEQYQLCALAINDECYQVRQVFAQKLHKGLSRLRLPLEYMAICALCAKDPVKERRAHARQ CLVKNINVRREYLKQHAAVSEKLLSLLPEYVVPYTIHLLAHDPDYVKVQDIEQLKDVKEC LWFVLEILMAKNENNSHAFIRKMVENIKQTKDAQGPDDAKMNEKLYTVCDVAMNIIMSKS TTYSLESPKDPVLPARFFTQPDKNFSNTKNYLPPEMKSFFTPGKPKTTNVLGAVNKPLSS AGKQSQTKSSRMETVSNASSSSNPSSPGRIKGRLDSSEMDHSENEDYTMSSPLPGKKSDK RDDSDLVRSELEKPRGRKKTPVTEQEEKLGMDDLTKLVQEQKPKGSQRSRKRGHTASESD EQQWPEEKRLKEDILENEDEQNSPPKKGKRGRPPKPLGGGTPKEEPTMKTSKKGSKKKSG PPAPEEEEEEERQSGNTEQKSKSKQHRVSRRAQQRAESPESSAIESTQSTPQKGRGRPSK TPSPSQPKKNVRVGRSKQAATKENDSSEEVDVFQGSSPVDDIPQEETEEEEVSTVNVRRR SAKRERR
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pGW_NcoI-Eco109I
Synonyms:
pGW-NcoEco
Description:
In-fusion cloning vector with NcoI (5'att) and Eco109I (3'att) sites inserted to remove death cassette; kanamycin resistance in bacteria; recombinational cloning
Comments:
NcoI (5'att) and Eco109I (3'att) sites inserted to remove death cassette. Made for use with In-Fusion cloning
Size (bp):
4008
Parent Vector:
None
Properties:
''
Author Name:
Joshua LaBaer
Andreas Rolf
Andreas Rolf
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 bacterial origin
3332
4005
death cassette
ccdB
ccdB death cassette - removed in plasmids containing an insert
1779
2084
primer
M13 forward
M13 forward sequencing primer
536
553
primer
M13 reverse
M13 reverse sequencing primer
2269
2289
recombination site
attL1
attL1 recombinational cloning site
644
668
recombination site
attL2
attL2 recombinational cloning site
2133
2157
selectable marker
KanR
kanamycin resistance
2402
3208
selectable marker
CmR
Chloramphenicol resistance - removed in plasmids containing an insert
778
1434
