Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
Codon optimized
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pGW_NcoI-Eco109I
Format:
FUSION
Source:
None
Description:
None
Comments:
Gene synthesis by Gen9. Codon optimized
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00744717
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 4971nts
Open reading frame: 1 to 4971
ATGGGACTTCAAGCCAGGCGATGGGCGTCTGGAAGCCGGGGCGCTGCTGGGCCGCGACGG
GGCGTCTTGCAGCTTCTTCCGCTTCCACTGCCTCTGCCGTTATTACTGCTGCTGCTGCTA
CGCCCAGGAGCCGGAAGGGCTGCGGCGCAGGGCGAGGCGGAGGCGCCCACACTCTATCTG
TGGAAGACAGGTCCATGGGGCCGATGTATGGGAGATGAATGTGGTCCCGGAGGCATCCAA
ACGAGGGCTGTGTGGTGTGCTCATGTCGAGGGATGGACTACACTGCATACTAACTGTAAG
CAGGCCGAGAGGCCCAATAACCAGCAGAATTGTTTCAAAGTTTGCGATTGGCACAAAGAG
TTGTACGACTGGAGACTGGGACCTTGGAATCAGTGTCAGCCCGTCATTTCAAAAAGTCTA
GAGAAACCTCTGGAGTGCATTAAGGGGGAAGAAGGTATTCAGGTGAGGGAGATAGCGTGC
ATCCAGAAAGACAAAGACATTCCTGCCGAGGATATCATCTGTGAGTACTTTGAGCCTAAG
CCTCTTCTGGAGCAGGCTTGCCTCATTCCTTGCCAGCAAGATTGCATCGTGTCTGAATTT
TCTGCTTGGTCCGAATGCTCCAAGACCTGCGGCAGCGGGCTCCAGCACCGGACACGGCAT
GTGGTGGCGCCCCCGCAGTTCGGAGGCTCTGGCTGTCCAAACCTGACGGAGTTCCAGGTG
TGCCAATCCAGTCCGTGCGAGGCCGAGGAGCTGAGGTACAGTCTGCATGTGGGGCCCTGG
AGCACTTGCTCAATGCCTCACTCCCGACAAGTAAGACAAGCAAGGAGACGCGGGAAGAAT
AAGGAACGGGAAAAGGACCGCAGCAAAGGAGTAAAGGATCCAGAAGCCCGCGAGCTTATT
AAGAAAAAGAGAAACAGAAACAGGCAGAACAGACAAGAGAACAAATATTGGGACATCCAG
ATTGGATATCAGACCAGAGAGGTTATGTGCATTAACAAGACGGGGAAAGCTGCTGATTTA
AGCTTTTGCCAGCAAGAGAAGCTTCCAATGACCTTCCAGTCCTGCGTGATCACCAAAGAG
TGCCAAGTTTCCGAGTGGTCAGAGTGGAGCCCCTGCTCAAAAACATGCCATGACATGGTG
TCCCCTGCAGGCACTCGGGTAAGGACACGAACCATCAGGCAGTTTCCGATTGGCAGTGAA
AAGGAGTGTCCAGAATTTGAAGAAAAAGAGCCCTGTTTGTCTCAAGGAGATGGAGTTGTC
CCCTGTGCCACCTATGGCTGGAGAACTACCGAGTGGACTGAGTGCCGTGTGGACCCTTTG
CTCAGTCAGCAGGACAAGAGGCGCGGCAACCAGACGGCCCTTTGTGGAGGGGGCATCCAG
ACCCGAGAGGTGTACTGCGTGCAGGCCAACGAAAACCTCCTCTCACAATTAAGTACCCAT
AAGAACAAAGAAGCCTCAAAGCCAATGGACTTAAAATTATGCACTGGACCTATCCCTAAT
ACTACACAGCTGTGCCACATTCCTTGTCCAACTGAATGTGAAGTTTCACCTTGGTCAGCT
TGGGGACCTTGTACTTATGAAAACTGTAATGACCAGCAAGGGAAAAAAGGCTTCAAACTT
AGGAAGCGCCGCATTACCAACGAGCCCACTGGAGGCTCTGGGGTAACCGGCAACTGCCCT
CACTTACTGGAAGCCATTCCCTGTGAAGAGCCTGCCTGTTATGACTGGAAAGCAGTGAGA
CTGGGAAACTGCGAGCCAGATAACGGAAAGGAGTGTGGTCCAGGCACGCAAGTGCAAGAG
GTTGTCTGCATCAACAGTGATGGAGAAGAAGTTGACAGACAGCTGTGCAGAGATGCCATC
TTCCCCATCCCTGTCGCCTGTGATGCCCCATGCCCGAAAGACTGTGTGCTCAGCACATGG
TCTACGTGGTCCTCCTGCTCCCACACCTGTTCAGGGAAAACGACAGAAGGGAAACAGATA
CGAGCTCGATCCATTCTGGCCTATGCGGGTGAAGAAGGTGGAATTCGCTGTCCAAATAGC
AGTGCTTTGCAAGAAGTAAGAAGCTGTAATGAGCATCCTTGCACAGTGTACCACTGGCAA
ACTGGTCCTTGGGGCCAGTGCATTGAGGACACCTCAGTATCGTCCTTCAACACAACTACG
ACTTGGAATGGGGAGGCCTCCTGCTCTGTCGGCATGCAGACAAGAAAAGTCATCTGTGTG
CGAGTCAATGTGGGCCAAGTGGGACCCAAAAAATGTCCCGAAAGCCTCCGACCTGAAACT
GTACGGCCTTGTCTGCTACCTTGTAAGAAGGACTGTATTGTGACCCCATATAGTGACTGG
ACATCATGCCCCTCTTCGTGTAAAGAAGGGGACTCCTCAATCAGGAAGCAGTCTAGGCAT
CGGGTCATCATTCAGCTGCCAGCCAACGGGGGCCGAGACTGCACAGATCCCCTCTATGAA
GAGAAGGCCTGTGAGGCCCCCCAAGCGTGCCAAAGCTACAGGTGGAAGACTCACAAATGG
CGCAGATGCCAATTAGTCCCTTGGAGCGTGCAACAAGACAGCCCTGGAGCACAGGAAGGA
TGTGGGCCTGGGAGACAGGCAAGAGCCATTACTTGTCGCAAGCAAGATGGAGGACAGGCT
GGAATTCATGAGTGCCTACAGTATGCAGGCCCTGTGCCAGCACTTACCCAGGCCTGCCAG
ATCCCCTGCCAGGATGACTGTCAATTGACTAGCTGGTCCAAATTTTCTTCATGCAATGGA
GACTGTGGTGCAGTTAGGACCAGAAAGCGCACTCTTGTTGGAAAAAGTAAAAAGAAGGAA
AAATGTAAAAATTCCCATCTCTATCCCCTGATTGAGACTCAGTATTGTCCTTGTGACAAA
TATAATGCCCAACCTGTGGGGAACTGGTCAGACTGTATTCTACCAGAGGGAAAAGTGGAA
GTGTTGCTTGGGATGAAAGTACAAGGCGACATCAAGGAATGCGGACAAGGATATCGTTAC
CAAGCAATGGCATGCTACGATCAAAATGGCAGGCTTGTGGAAACATCTAGATGTAACAGC
CATGGTTACATTGAGGAGGCCTGCATCATCCCCTGCCCCTCAGACTGCAAGCTCAGTGAG
TGGTCCAACTGGTCGCGCTGCAGCAAGTCTTGTGGGAGTGGTGTCAAGGTTCGTTCTAAA
TGGCTCCGTGAAAAACCATATAATGGCGGAAGGCCTTGCCCCAAACTGGACCATGTCAAC
CAGGCACAGGTGTATGAGGTTGTCCCCTGCCACAGCGACTGCAACCAGTACCTATGGGTG
ACAGAGCCCTGGAGCATCTGCAAGGTCACCTTTGTGAATATGCGGGAGAACTGTGGCGAG
GGCGTGCAAACCCGAAAAGTGAGATGCATGCAGAATACAGCCGATGGCCCTTCTGAACAT
GTAGAGGATTACCTCTGTGACCCAGAAGAGATGCCACTGGGCTCAAGAGTGTGCAAATTA
CCATGCCCTGAGGACTGTGTAATATCAGAATGGGGTCCATGGACCCAATGTGTTCTGCCT
TGCAATCAATCCAGTTTCCGGCAAAGGTCAGCTGATCCCATCAGACAACCAGCTGATGAA
GGACGATCTTGCCCTAATGCTGTTGAGAAAGAACCCTGTAACCTGAACAAAAACTGCTAC
CACTATGATTATAATGTCACAGACTGGAGTACATGTCAGCTGAGTGAGAAGGCAGTTTGT
GGAAATGGAATAAAAACAAGGATGTTGGATTGTGTTCGAAGTGATGGCAAGTCAGTTGAC
CTGAAATATTGTGAAGCCCTTGGCTTGGAGAAGAACTGGCAGATGAACACGTCCTGCATG
GTGGAATGCCCCGTGAACTGTCAGCTTTCTGATTGGTCCCCCTGGTCAGAATGTTCTCAA
ACATGTGGCCTCACAGGAAAAATGATCCGGAGACGAACAGTGACCCAGCCCTTTCAAGGT
GATGGAAGACCATGCCCTTCCCTGATGGACCAGTCCAAACCCTGCCCAGTGAAGCCTTGT
TATCGGTGGCAATATGGCCAGTGGTCCCCATGTCAAGTGCAGGAGGCCCAGTGTGGAGAA
GGGACCCGAACAAGGAACATTTCTTGTGTAGTAAGTGATGGCTCCGCCGATGATTTCAGT
AAAGTGGTGGATGAGGAATTCTGTGCCGACATTGAACTCATTATAGATGGTAATAAAAAT
ATGGTTCTGGAGGAATCCTGCAGCCAGCCTTGCCCAGGTGACTGTTATTTGAAGGACTGG
TCTTCCTGGAGCCTGTGTCAGCTGACCTGTGTGAATGGTGAGGATCTAGGCTTTGGTGGA
ATCCAGGTCCGATCCAGGCCGGTGATTATACAAGAACTAGAGAATCAGCATCTGTGCCCA
GAGCAGATGTTAGAAACTAAATCATGTTATGATGGACAGTGCTATGAATACAAATGGATG
GCCAGTGCTTGGAAGGGCTCTTCCCGAACAGTGTGGTGTCAAAGGTCAGATGGTATAAAT
GTAACAGGGGGCTGCTTGGTGATGAGCCAGCCTGATGCCGACAGAAGTTGTAACCCACCG
TGTAGTCAACCCCATTCGTACTGTAGCGAGACAAAAACATGCCATTGTGAAGAAGGGTAC
ACTGAAGTCATGTCTTCTAACAGCACCCTTGAGCAATGCACACTTATCCCCGTGGTGGTA
CTTCCCACCATGGAGGACAAAAGAGGCGATGTGAAAACCAGTCGGGCTGTACATCCAACC
CAACCCTCCAGTAACCCAGCAGGACGGGGAAGGACCTGGTTTCTACAGCCATTTGGGCCA
GATGGGAGACTAAAGACATGGGTTTACGGTGTAGCAGCTGGTGCATTTGTGTTACTCATC
TTTATTGTCTCCATGATCTATCTAGCTTGCAAAAAGCCAAAGAAACCCCAGAGAAGGCAA
AACAACCGACTGAAACCTTTAACCTTAGCCTATGATGGCGATGCCGACATG
Protein sequence predicted based on nucleotide sequence
MGLQARRWASGSRGAAGPRRGVLQLLPLPLPLPLLLLLLLRPGAGRAAAQGEAEAPTLYL WKTGPWGRCMGDECGPGGIQTRAVWCAHVEGWTTLHTNCKQAERPNNQQNCFKVCDWHKE LYDWRLGPWNQCQPVISKSLEKPLECIKGEEGIQVREIACIQKDKDIPAEDIICEYFEPK PLLEQACLIPCQQDCIVSEFSAWSECSKTCGSGLQHRTRHVVAPPQFGGSGCPNLTEFQV CQSSPCEAEELRYSLHVGPWSTCSMPHSRQVRQARRRGKNKEREKDRSKGVKDPEARELI KKKRNRNRQNRQENKYWDIQIGYQTREVMCINKTGKAADLSFCQQEKLPMTFQSCVITKE CQVSEWSEWSPCSKTCHDMVSPAGTRVRTRTIRQFPIGSEKECPEFEEKEPCLSQGDGVV PCATYGWRTTEWTECRVDPLLSQQDKRRGNQTALCGGGIQTREVYCVQANENLLSQLSTH KNKEASKPMDLKLCTGPIPNTTQLCHIPCPTECEVSPWSAWGPCTYENCNDQQGKKGFKL RKRRITNEPTGGSGVTGNCPHLLEAIPCEEPACYDWKAVRLGNCEPDNGKECGPGTQVQE VVCINSDGEEVDRQLCRDAIFPIPVACDAPCPKDCVLSTWSTWSSCSHTCSGKTTEGKQI RARSILAYAGEEGGIRCPNSSALQEVRSCNEHPCTVYHWQTGPWGQCIEDTSVSSFNTTT TWNGEASCSVGMQTRKVICVRVNVGQVGPKKCPESLRPETVRPCLLPCKKDCIVTPYSDW TSCPSSCKEGDSSIRKQSRHRVIIQLPANGGRDCTDPLYEEKACEAPQACQSYRWKTHKW RRCQLVPWSVQQDSPGAQEGCGPGRQARAITCRKQDGGQAGIHECLQYAGPVPALTQACQ IPCQDDCQLTSWSKFSSCNGDCGAVRTRKRTLVGKSKKKEKCKNSHLYPLIETQYCPCDK YNAQPVGNWSDCILPEGKVEVLLGMKVQGDIKECGQGYRYQAMACYDQNGRLVETSRCNS HGYIEEACIIPCPSDCKLSEWSNWSRCSKSCGSGVKVRSKWLREKPYNGGRPCPKLDHVN QAQVYEVVPCHSDCNQYLWVTEPWSICKVTFVNMRENCGEGVQTRKVRCMQNTADGPSEH VEDYLCDPEEMPLGSRVCKLPCPEDCVISEWGPWTQCVLPCNQSSFRQRSADPIRQPADE GRSCPNAVEKEPCNLNKNCYHYDYNVTDWSTCQLSEKAVCGNGIKTRMLDCVRSDGKSVD LKYCEALGLEKNWQMNTSCMVECPVNCQLSDWSPWSECSQTCGLTGKMIRRRTVTQPFQG DGRPCPSLMDQSKPCPVKPCYRWQYGQWSPCQVQEAQCGEGTRTRNISCVVSDGSADDFS KVVDEEFCADIELIIDGNKNMVLEESCSQPCPGDCYLKDWSSWSLCQLTCVNGEDLGFGG IQVRSRPVIIQELENQHLCPEQMLETKSCYDGQCYEYKWMASAWKGSSRTVWCQRSDGIN VTGGCLVMSQPDADRSCNPPCSQPHSYCSETKTCHCEEGYTEVMSSNSTLEQCTLIPVVV LPTMEDKRGDVKTSRAVHPTQPSSNPAGRGRTWFLQPFGPDGRLKTWVYGVAAGAFVLLI FIVSMIYLACKKPKKPQRRQNNRLKPLTLAYDGDADM
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pGW_NcoI-Eco109I
Synonyms:
pGW-NcoEco
Description:
In-fusion cloning vector with NcoI (5'att) and Eco109I (3'att) sites inserted to remove death cassette; kanamycin resistance in bacteria; recombinational cloning
Comments:
NcoI (5'att) and Eco109I (3'att) sites inserted to remove death cassette. Made for use with In-Fusion cloning
Size (bp):
4008
Parent Vector:
None
Properties:
''
Author Name:
Joshua LaBaer
Andreas Rolf
Andreas Rolf
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 bacterial origin
3332
4005
death cassette
ccdB
ccdB death cassette - removed in plasmids containing an insert
1779
2084
primer
M13 forward
M13 forward sequencing primer
536
553
primer
M13 reverse
M13 reverse sequencing primer
2269
2289
recombination site
attL1
attL1 recombinational cloning site
644
668
recombination site
attL2
attL2 recombinational cloning site
2133
2157
selectable marker
KanR
kanamycin resistance
2402
3208
selectable marker
CmR
Chloramphenicol resistance - removed in plasmids containing an insert
778
1434
