Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
Codon optimized
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
None
Description:
None
Comments:
Gene synthesis by Gen9. Codon optimized
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00744786
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 5058nts
Open reading frame: 1 to 5058
ATGCCCGGCGGCCCCAGTCCTCGAAGCCCTGCGCCTTTACTTCGACCTCTCCTCCTGCTT
CTCTGCGCTCTGGCTCCTGGAGCCCCCGGACCTGCACCAGGACGTGCAACCGAAGGACGG
GCGGCACTGGACATCGTTCACCCTGTTCGAGTCGACGCGGGAGGCTCCTTCCTGTCCTAC
GAGCTGTGGCCCCGCGCACTGCGCAAGCGGGATGTATCTGTGCGCCGAGACGCGCCCGCT
TTCTACGAGCTACAATACCGCGGGCGCGAGCTGCGCTTCAACCTTACCGCCAATCAGCAC
CTTCTGGCGCCCGGCTTTGTGAGCGAGACACGGCGGCGCGGCGGACTTGGACGAGCTCAC
ATTCGGGCCCACACACCGGCCTGCCATCTTCTTGGCGAGGTGCAGGACCCTGAGCTTGAG
GGTGGCCTGGCGGCCATCAGCGCCTGCGACGGCCTGAAAGGTGTGTTCCAGCTCTCCAAC
GAGGACTACTTCATTGAGCCCCTGGACAGTGCCCCGGCCCGGCCTGGCCACGCCCAACCC
CATGTGGTGTACAAGCGTCAGGCCCCGGAGAGACTGGCACAGCGGGGTGATTCTAGTGCT
CCAAGCACCTGTGGAGTGCAAGTGTACCCAGAGCTGGAGTCTCGACGGGAGCGTTGGGAG
CAGCGGCAGCAGTGGCGGCGGCCACGGCTGAGGCGTCTACACCAGAGGTCGGTCAGCAAA
GAGAAGTGGGTGGAAACCCTGGTAGTAGCTGATGCCAAAATGGTGGAGTACCACGGACAG
CCGCAGGTTGAGAGCTATGTGCTGACCATCATGAACATGGTGGCTGGCCTGTTTCATGAC
CCCAGCATTGGGAACCCCATCCACATCACCATTGTGCGCCTGGTCCTGCTGGAAGATGAG
GAGGAGGACCTAAAGATCACGCACCATGCAGACAACACCCTGAAGAGCTTCTGCAAGTGG
CAGAAAAGCATCAACATGAAGGGGGATGCCCATCCCCTGCACCATGACACTGCCATCCTG
CTCACCAGAAAGGACCTGTGTGCAGCCATGAACCGGCCCTGTGAAACCCTGGGACTGTCC
CATGTGGCGGGCATGTGCCAGCCGCACCGCAGCTGCAGCATCAACGAGGACACGGGCCTG
CCGCTGGCCTTCACTGTAGCCCACGAGCTCGGGCACAGTTTTGGCATTCAGCATGACGGA
AGCGGCAATGACTGTGAGCCCGTTGGGAAACGACCTTTCATCATGTCTCCACAGCTCCTG
TACGACGCCGCTCCCCTCACCTGGTCCCGCTGCAGCCGCCAGTATATCACCAGGTTCCTT
GACCGTGGGTGGGGCCTGTGCCTGGACGACCCTCCTGCCAAGGACATTATCGACTTCCCC
TCGGTGCCACCTGGCGTCCTCTATGATGTAAGCCACCAGTGCCGCCTCCAGTACGGGGCC
TACTCTGCCTTCTGCGAGGACATGGATAATGTCTGCCACACACTCTGGTGCTCTGTGGGG
ACCACCTGTCACTCCAAGCTGGATGCAGCTGTGGACGGCACCCGGTGTGGGGAGAATAAG
TGGTGTCTCAGTGGGGAGTGCGTACCCGTGGGCTTCCGGCCTGAGGCCGTGGATGGTGGC
TGGTCTGGCTGGAGCGCCTGGTCCATCTGCTCCAGGAGTTGTGGCATGGGCGTACAGAGC
GCCGAGCGGCAGTGCACGCAGCCTACGCCCAAATACAAAGGCAGATACTGTGTGGGTGAG
CGCAAGCGCTTCCGCCTCTGCAACCTGCAGGCCTGCCCTGCTGGCCGCCCCTCCTTCCGC
CACGTCCAGTGCAGCCACTTTGACGCTATGCTCTACAAGGGCCAGCTGCACACATGGGTG
CCCGTGGTCAATGACGTGAACCCCTGCGAGCTGCACTGCCGGCCCGCGAATGAGTACTTT
GCCGAGAAGCTGCGGGACGCCGTGGTCGATGGCACCCCCTGCTACCAGGTCCGAGCCAGC
CGGGACCTCTGCATCAACGGCATCTGTAAGAACGTGGGCTGTGACTTCGAGATTGACTCC
GGTGCTATGGAGGACCGCTGTGGTGTGTGCCACGGCAACGGCTCTACCTGCCACACCGTG
AGCGGGACCTTCGAGGAGGCCGAGGGCCTGGGGTATGTGGATGTGGGGCTTATCCCAGCG
GGCGCACGCGAGATCCGCATCCAAGAGGTTGCCGAGGCTGCCAACTTCCTGGCACTGCGG
AGCGAGGACCCGGAGAAGTACTTCCTCAATGGTGGCTGGACCATCCAGTGGAACGGGGAC
TACCAGGTGGCAGGGACCACCTTCACATACGCACGCAGGGGCAACTGGGAGAACCTCACG
TCCCCGGGTCCCACCAAGGAGCCTGTCTGGATCCAGCTGCTGTTCCAGGAGAGCAACCCT
GGGGTGCACTACGAGTACACCATCCACAGGGAGGCAGGCGGCCACGACGAGGTCCCGCCG
CCCGTGTTCTCCTGGCATTATGGGCCCTGGACCAAGTGCACAGTCACATGCGGCAGAGGT
GTGCAGAGGCAGAATGTGTACTGCTTGGAGAGGCAGGCAGGGCCCGTGGACGAGGAGCAC
TGTGACCCCCTGGGCCGGCCTGATGACCAACAGAGGAAGTGCAGCGAACAACCTTGCCCT
GCCAGGTGGTGGGCCGGTGAGTGGCAGCTGTGTTCCAGCTCCTGCGGACCTGGGGGCCTC
TCTCGCCGGGCCGTGCTTTGCATCAGAAGTGTGGGGCTGGATGAGCAGAGCGCTCTGGAG
CCCCCTGCCTGTGAACACCTTCCCCGGCCCCCTACTGAAACCCCTTGCAACCGGCATGTA
CCCTGTCCGGCCACCTGGGCTGTGGGGAACTGGTCCCAGTGCTCCGTGACATGTGGGGAG
GGCACTCAGCGCCGAAATGTCCTCTGCACCAATGACACCGGTGTCCCCTGTGACGAGGCC
CAGCAGCCAGCCAGCGAAGTCACATGCTCTCTGCCACTCTGTCGGTGGCCCCTGGGCACA
CTGGGCCCTGAAGGCTCAGGCAGCGGCTCCTCCAGCCACGAGCTCTTCAACGAGGCTGAC
TTCATCCCGCACCACCTGGCCCCACGCCCTTCACCCGCCTCATCACCCAAGCCAGGCACC
ATGGGCAACGCTATTGAGGAGGAGGCTCCAGAGCTGGACCTGCCGGGGCCCGTGTTTGTG
GACGACTTCTACTACGACTACAATTTCATCAATTTCCACGAGGATCTGTCCTACGGGCCC
TCTGAGGAGCCCGATCTAGACCTGGCGGGGACAGGGGATCGGACACCCCCACCACACAGC
CATCCTGCTGCGCCCTCCACAGGTAGTCCCGTGCCTGCCACAGAGCCTCCTGCAGCCAAG
GAGGAAGGGGTACTTGGACCTTGGTCCCCGAGCCCTTGGCCTAGCCAAGCCGGCCGATCC
CCACCCCCACCCTCAGAGCAGACCCCTGGGAACCCTTTGATCAATTTCCTGCCTGAGGAA
GACACCCCCATAGGGGCCCCAGATCTTGGGCTCCCCAGCCTGTCCTGGCCCAGGGTTTCC
ACTGATGGCCTGCAGACACCCGCCACCCCTGAGAGCCAAAATGATTTCCCAGTTGGCAAG
GACAGCCAGAGCCAGCTGCCCCCTCCATGGCGGGACAGGACCAATGAGGTTTTCAAGGAT
GATGAGGAACCCAAGGGCCGAGGAGCACCCCACCTCCCTCCGAGGCCCAGTTCCACGCTT
CCCCCTCTGTCCCCTGTTGGCAGCACCCACTCCTCTCCTAGTCCTGACGTGGCGGAGCTG
TGGACAGGAGGCACAGTGGCCTGGGAGCCAGCTCTGGAGGGTGGCCTGGGGCCTGTGGAC
AGTGAACTGTGGCCCACTGTTGGGGTGGCTTCTCTCCTTCCTCCTCCCATAGCCCCTCTG
CCAGAGATGAAGGTCAGGGACAGTTCCCTGGAGCCGGGGACTCCCTCCTTCCCAACCCCA
GGACCAGGCTCATGGGACCTGCAGACTGTGGCAGTGTGGGGGACCTTCCTCCCCACAACC
CTGACTGGCCTCGGGCACATGCCTGAGCCTGCCCTGAACCCAGGACCCAAGGGTCAGCCT
GAGTCCCTGAGCCCTGAGGTGCCCCTGAGCTCTAGGCTGCTGTCCACACCAGCTTGGGAC
AGCCCCGCCAACAGCCACAGAGTCCCTGAAACCCAGCCGCTTGCTCCCAGTCTGGCTGAA
GCGGGGCCTCCCGCGGACCCGTTGGTTGTCAGGAACGCCGGCTGGCAAGCGGGAAACTGG
AGCGAGTGCTCCACCACCTGTGGCCTGGGTGCTGTCTGGAGGCCAGTTCGCTGTAGTTCC
GGACGGGATGAGGACTGCGCTCCCGCTGGACGGCCTCAGCCTGCTCGCCGCTGCCATCTG
CGGCCTTGTGCCACCTGGCACTCAGGAAACTGGAGTAAGTGCTCCCGCAGCTGCGGCGGA
GGTTCCTCAGTGCGGGACGTGCAGTGTGTGGATACACGGGACCTCAGGCCACTGCGGCCT
TTTCATTGTCAGCCCGGGCCTGCTAAGCCGCCTGCGCACAGGCCCTGCGGGGCCCAGCCT
TGCCTCAGCTGGTACACATCCTCCTGGAGAGAGTGTTCTGAGGCCTGTGGCGGTGGTGAG
CAGCAGCGTCTAGTGACCTGTCCGGAGCCAGGCCTCTGCGAGGAGGCGCTCAGACCCAAC
ACAACCCGGCCCTGCAACACCCACCCCTGCACGCAGTGGGTGGTTGGACCTTGGGGACAG
TGCTCAGGCCCCTGTGGTGGTGGTGTCCAGCGGCGCCTGGTCAAGTGTGTCAACACCCAG
ACAGGGCTGCCCGAGGAAGACAGTGACCAGTGTGGCCACGAGGCCTGGCCTGAGAGCTCC
AGGCCGTGTGGCACCGAGGATTGTGAGCCCGTTGAGCCTCCCCGATGTGAGCGGGACCGC
CTGTCCTTCGGGTTCTGCGAAACGCTGCGCCTACTGGGCCGCTGCCAGCTGCCCACCATC
CGCACACAGTGCTGTCGCTCTTGCTCTCCGCCTAGCCACGGAGCCCCTTCCCGAGGACAT
CAGCGGGTTGCCCGACGC
Protein sequence predicted based on nucleotide sequence
MPGGPSPRSPAPLLRPLLLLLCALAPGAPGPAPGRATEGRAALDIVHPVRVDAGGSFLSY ELWPRALRKRDVSVRRDAPAFYELQYRGRELRFNLTANQHLLAPGFVSETRRRGGLGRAH IRAHTPACHLLGEVQDPELEGGLAAISACDGLKGVFQLSNEDYFIEPLDSAPARPGHAQP HVVYKRQAPERLAQRGDSSAPSTCGVQVYPELESRRERWEQRQQWRRPRLRRLHQRSVSK EKWVETLVVADAKMVEYHGQPQVESYVLTIMNMVAGLFHDPSIGNPIHITIVRLVLLEDE EEDLKITHHADNTLKSFCKWQKSINMKGDAHPLHHDTAILLTRKDLCAAMNRPCETLGLS HVAGMCQPHRSCSINEDTGLPLAFTVAHELGHSFGIQHDGSGNDCEPVGKRPFIMSPQLL YDAAPLTWSRCSRQYITRFLDRGWGLCLDDPPAKDIIDFPSVPPGVLYDVSHQCRLQYGA YSAFCEDMDNVCHTLWCSVGTTCHSKLDAAVDGTRCGENKWCLSGECVPVGFRPEAVDGG WSGWSAWSICSRSCGMGVQSAERQCTQPTPKYKGRYCVGERKRFRLCNLQACPAGRPSFR HVQCSHFDAMLYKGQLHTWVPVVNDVNPCELHCRPANEYFAEKLRDAVVDGTPCYQVRAS RDLCINGICKNVGCDFEIDSGAMEDRCGVCHGNGSTCHTVSGTFEEAEGLGYVDVGLIPA GAREIRIQEVAEAANFLALRSEDPEKYFLNGGWTIQWNGDYQVAGTTFTYARRGNWENLT SPGPTKEPVWIQLLFQESNPGVHYEYTIHREAGGHDEVPPPVFSWHYGPWTKCTVTCGRG VQRQNVYCLERQAGPVDEEHCDPLGRPDDQQRKCSEQPCPARWWAGEWQLCSSSCGPGGL SRRAVLCIRSVGLDEQSALEPPACEHLPRPPTETPCNRHVPCPATWAVGNWSQCSVTCGE GTQRRNVLCTNDTGVPCDEAQQPASEVTCSLPLCRWPLGTLGPEGSGSGSSSHELFNEAD FIPHHLAPRPSPASSPKPGTMGNAIEEEAPELDLPGPVFVDDFYYDYNFINFHEDLSYGP SEEPDLDLAGTGDRTPPPHSHPAAPSTGSPVPATEPPAAKEEGVLGPWSPSPWPSQAGRS PPPPSEQTPGNPLINFLPEEDTPIGAPDLGLPSLSWPRVSTDGLQTPATPESQNDFPVGK DSQSQLPPPWRDRTNEVFKDDEEPKGRGAPHLPPRPSSTLPPLSPVGSTHSSPSPDVAEL WTGGTVAWEPALEGGLGPVDSELWPTVGVASLLPPPIAPLPEMKVRDSSLEPGTPSFPTP GPGSWDLQTVAVWGTFLPTTLTGLGHMPEPALNPGPKGQPESLSPEVPLSSRLLSTPAWD SPANSHRVPETQPLAPSLAEAGPPADPLVVRNAGWQAGNWSECSTTCGLGAVWRPVRCSS GRDEDCAPAGRPQPARRCHLRPCATWHSGNWSKCSRSCGGGSSVRDVQCVDTRDLRPLRP FHCQPGPAKPPAHRPCGAQPCLSWYTSSWRECSEACGGGEQQRLVTCPEPGLCEEALRPN TTRPCNTHPCTQWVVGPWGQCSGPCGGGVQRRLVKCVNTQTGLPEEDSDQCGHEAWPESS RPCGTEDCEPVEPPRCERDRLSFGFCETLRLLGRCQLPTIRTQCCRSCSPPSHGAPSRGH QRVARR
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
