Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
Codon optimized
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
None
Description:
None
Comments:
Gene synthesis by Gen9. Codon optimized
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00744791
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 6789nts
Open reading frame: 1 to 6789
ATGATGTTGCTAATTTTGTTCCTCGTGATAATTTGTTCCCATATTTCTGTGAACCAAGAT
TCCGGCCCTGAGTACGCAGATGTTGTGTTTTTGGTGGACAGCTCTGATCGCCTGGGATCC
AAGTCCTTCCCATTTGTTAAAATGTTCATTACCAAAATGATCAGCAGTCTCCCCATAGAG
GCCGACAAATACCGTGTGGCCCTGGCCCAGTACAGTGATAAACTTCACAGTGAATTCCAC
CTGAGCACCTTCAAAGGCAGGAGCCCCATGCTGAACCACCTAAGGAAGAACTTTGGATTC
ATTGGCGGGTCCCTGCAGATAGGAAAGGCTCTTCAGGAGGCTCACAGGACTTATTTCTCT
GCACCCGCAAATGGGAGAGACAAGAAACAGTTTCCCCCAATCCTGGTGGTCCTGGCTTCA
TCTGAGTCTGAGGATAATGTGGAAGAGGCATCAAAGGCCCTGCGGAAAGACGGAGTGAAA
ATCATCTCTGTAGGGGTGCAGAAAGCTTCCGAGGAAAACCTCAAGGCCATGGCCACGTCT
CAGTTTCATTTCAACCTTCGGACAGTCAGAGATCTCAGCATGTTTTCCCAAAACATGACA
CACATCATCAAGGATGTAATAAAGTACAAGGAGGGAGCAGTTGATGACATCTTTGTAGAA
GCTTGCCAAGGCCCTTCTATGGCCGATGTTGTGTTCCTATTGGATATGTCAATCAATGGA
AGTGAGGAGAACTTTGACTATCTTAAAGGATTCTTGGAAGAAAGTGTATCTGCCCTTGAC
ATAAAGGAAAATTGCATGAGGGTTGGCCTTGTGGCCTATAGCAATGAGACAAAAGTGATA
AATTCACTGAGCATGGGCATAAATAAGTCAGAGGTTCTCCAGCATATACAGAACCTTTCT
CCCCGAACTGGGAAGGCTTATACTGGAGCTGCCATCAAAAAGCTCAGGAAGGAAGTTTTT
AGTGCACGGAATGGCAGTCGGAAGAATCAGGGGGTGCCCCAGATTGCCGTGCTGGTGACC
CACCGAGATTCAGAAGACAACGTGACAAAAGCAGCTGTTAACCTCCGACGGGAGGGTGTG
ACCATCTTCACCCTGGGCATAGAGGGCGCCAGCGACACCCAGTTGGAAAAGATAGCATCC
CACCCTGCTGAGCAGTATGTCTCCAAACTGAAGACATTCGCTGACCTGGCTGCTCACAAC
CAGACATTTCTGAAGAAGCTGCGGAACCAAATAACACACACAGTCTCTGTCTTTTCAGAG
AGGACTGAAACGCTCAAATCTGGTTGTGTGGACACTGAGGAAGCAGACATCTATCTGCTT
ATCGATGGCTCAGGGAGCACCCAGGCCACAGATTTCCATGAAATGAAGACGTTCCTGTCA
GAGGTGGTAGGGATGTTCAACATTGCTCCCCATAAGGTGAGGGTTGGGGCCGTTCAGTAT
GCTGACAGCTGGGACTTGGAATTTGAGATCAATAAATACTCCAACAAGCAGGATTTGGGA
AAGGCCATTGAGAATATCAGGCAGATGGGCGGGAATACAAACACAGGCGCAGCACTGAAT
TTCACACTGAGCCTGTTGCAAAAAGCAAAGAAGCAGAGAGGAAACAAAGTTCCATGCCAC
CTTGTTGTCCTGACAAATGGCATGTCCAAGGATAGCATCTTGGAGCCTGCAAACAGACTG
AGAGAAGAGCACATCCGAGTTTATGCTATCGGGATCAAGGAGGCCAACCAAACACAGCTG
AGAGAAATTGCAGGAGAGGAAAAGAGAGTGTATTACGTGCATGACTTTGATGCATTGAAA
GACATAAGAAACCAAGTTGTGCAAGAAATCTGTACTGAAGAAGCTTGCAAAGAGATGAAA
GCTGACATCATGTTTCTGGTGGACAGTTCTGGAAGTATAGGACCTGAAAACTTCAGCAAA
ATGAAAACATTTATGAAAAACCTGGTGAGCAAGTCTCAGATTGGACCAGATAGGGTGCAA
ATTGGTGTAGTCCAGTTCAGCGACATCAATAAGGAAGAGTTTCAGCTCAACAGATTCATG
TCCCAAAGCGACATCTCAAATGCAATAGACCAAATGGCTCACATTGGACAAACCACCCTG
ACTGGTAGTGCCCTGAGCTTTGTGTCACAGTACTTCAGCCCCACCAAGGGAGCCCGGCCC
AACATCAGAAAGTTTCTCATCCTCATCACGGATGGTGAAGCTCAGGACATAGTAAAGGAA
CCAGCAGTAGTGCTTCGGCAAGAAGGTGTAATCATCTATTCTGTGGGAGTGTTTGGCTCC
AATGTCACCCAGCTTGAGGAGATCAGTGGGAGGCCCGAGATGGTTTTTTATGTTGAGAAT
TTTGACATTCTGCAGCGCATTGAAGATGATCTTGTTTTTGGAATATGCAGCCCCCGTGAA
GAATGCAAGCGGATTGAAGTTTTAGACGTTGTGTTTGTCATTGATAGCTCCGGCTCCATT
GACTATGATGAGTATAATATCATGAAGGATTTTATGATTGGCTTAGTGAAAAAAGCTGAT
GTGGGCAAGAATCAGGTCCGGTTTGGGGCTCTGAAGTATGCTGATGACCCAGAGGTGCTG
TTTTATCTGGATGACTTTGGCACAAAACTGGAGGTAATTTCAGTGCTCCAGAATGACCAA
GCCATGGGCGGCAGTACTTATACTGCCGAGGCACTGGGCTTCTCAGACCACATGTTCACT
GAAGCCCGGGGCAGCCGCCTGAACAAGGGGGTCCCCCAAGTCCTTATTGTGATCACCGAT
GGGGAATCCCATGATGCTGATAAACTCAATGCCACGGCAAAGGCCTTGCGGGACAAAGGC
ATTCTTGTCCTGGCTGTGGGGATTGATGGTGCCAATCCCGTGGAGCTGTTAGCCATGGCA
GGATCAAGCGACAAGTACTTCTTCGTGGAGACTTTTGGAGGTCTGAAGGGAATATTTTCA
GACGTGACAGCCAGTGTCTGCAACTCTTCCAAAGTAGATTGTGAAATTGACAAGGTAGAT
CTTGTTTTCCTTATGGATGGTTCAACTAGCATTCAGCCAAATGACTTCAAGAAAATGAAG
GAATTTCTGGCATCTGTTGTTCAAGACTTTGATGTCAGCCTCAACAGAGTGCGAATAGGA
GCGGCCCAGTTTAGCGATACCTATCACCCGGAGTTTCCACTGGGAACTTTCATAGGTGAA
AAAGAGATATCATTTCAGATTGAAAACATCAAGCAGATCTTTGGAAACACACACATCGGT
GCTGCACTCAGGGAGGTGGAACATTACTTCAGGCCAGACATGGGCAGCAGGATAAATACA
GGTACCCCACAGGTGCTCCTGGTCCTTACAGATGGCCAGTCCCAAGACGAGGTGGCCCAG
GCCGCGGAAGCTCTGAGACACAGAGGTATCGACATCTACTCCGTGGGCATTGGGGATGTG
GATGACCAGCAGCTCATTCAGATCACCGGGACCGCAGAGAAAAAACTGACAGTGCACAAC
TTCGATGAACTGAAGAAGGTCAATAAAAGGATCGTTCGCAACATCTGTACAACAGCGGGT
GAAAGCAACTGTTTCGTGGATGTTGTGGTGGGATTTGATGTCTCAACTCAGGAGAAAGGG
CAGACTTTGCTTGAAGGTCAGCCTTGGATGGAAACCTACCTTCAAGACATCTTACGTGCC
ATCAGCTCCCTCAATGGAGTAAGCTGTGAGGTGGGCACAGAGACTCAGGTCAGTGTGGCT
TTTCAAGTGACCAATGCCATGGAAAAATATTCTCCCAAGTTTGAGATCTACAGTGAAAAC
ATACTGAATAGCTTGAAGGATATAACAGTTAAAGGACCATCTCTTCTCAATGCAAACCTC
TTGGATTCTCTATGGGATACATTTCAGAATAAATCAGCTGCTCGAGGCAAGGTGGTCCTT
TTATTTTCAGATGGATTGGATGATGATGTTGAGAAACTTGAACAAAAATCTGATGAACTT
AGAAAAGAAGGCCTGAATGCCCTCATAACTGTTGCTCTGGATGGACCTGCTGATTCAAGT
GACTTGGCTGATCTTCCCTATATTGAATTTGGGAAAGGATTTGAGTACAGGACACAGCTC
TCTATTGGCATGAGAGAACTTGGAAGCCGGCTGTCAAAGCAGCTGGTCAATGTTGCTGAA
AGGACATGCTGCTGTTTGTTCTGCAAGTGCATTGGAGGAGATGGCACAATGGGAGATCCT
GGACCACCAGGGAAAAGGGGACCTCCAGGTTTTAAAGGCAGTGAAGGCTACCTGGGAGAG
GAGGGAATCGCTGGAGAAAGAGGAGCCCCTGGACCAGTGGGAGAGCAAGGTACTAAGGGA
TGCTATGGCACCAAAGGTCCTAAGGGAAACAGAGGACTAAATGGACAGGAGGGAGAAGTT
GGGGAAAATGGAATTGACGGATTGAACGGAGAACAGGGTGATAATGGTCTTCCTGGAAGA
AAAGGAGAAAAGGGAGATGAGGGATCTCAGGGAAGCCCAGGGAAGAGAGGGACTCCTGGT
GACCGTGGAGCAAAGGGCCTGCGAGGGGATCCCGGAGCTCCTGGAGTTGACAGTAGCATA
GAAGGACCCACAGGCTTGAAAGGAGAACGTGGAAGACAAGGCAGAAGAGGCTGGCCAGGC
CCCCCCGGGACACCAGGCTCCAGAAGGAAGACAGCAGCTCATGGCAGAAGGGGACATACA
GGCCCACAGGGAACAGCAGGCATCCCAGGACCAGATGGACTTGAAGGCTCCCTGGGACTT
AAGGGCCCTCAGGGCCCAAGAGGAGAGGCTGGTGTGAAAGGAGAAAAAGGAGGTGTGGGA
AGTAAAGGTCCCCAGGGGCCTCCAGGACCCGGAGGAGAGGCAGGGAATCAAGGCCGTTTG
GGAAGCCAAGGAAATAAAGGAGAACCTGGAGATCTCGGAGAAAAAGGAGCTGTTGGCTTT
CCTGGTCCTCGTGGCTTGCAGGGCAATGATGGCAGTCCAGGTTATGGTAGTGTCGGACGC
AAGGGAGCAAAGGGACAAGAAGGATTCCCTGGAGAAAGTGGACCTAAGGGTGAGATTGGG
GACCCTGGTGGTCCAGGCGAGACTGGGCTGAAGGGAGCTAGAGGCAAAATGATATCTGCT
GGGCTTCCAGGAGAGATGGGATCCCCTGGGGAACCAGGACCTCCTGGACGTAAGGGTGTG
AAAGGAGCCAAAGGCTTGGCTTCATTTTCTACATGTGAGCTCATTCAGTATGTGCGAGAT
CGCAGTCCTGGCAGGCATGGAAAACCGGAATGCCCAGTGCACCCAACCGAGTTGGTGTTT
GCCCTGGACCACTCCCGGGATGTCACTGAGCAGGAATTTGAGCGGATGAAGGAGATGATG
GCTTTCCTGGTGAGAGACATTAAGGTGCGGGAGAACAGCTGCCCCGTGGGAGCGCACATC
GCCATCCTCTCCTATAACTCCCACGCCAGGCACCTTGTGCGCTTCTCAGACGCCTACAAG
AAGAGTCAACTTCTCAGAGAAATTGAAACTATTCCTTATGAGAGATCCTCTGCCAGCAGG
GAGATTGGCAGGGCAATGCGGTTTATTTCCAGGAATGTCTTCAAGCGGACGCTTCCGGGG
GCACACACGAGAAAAATTGCCACATTTTTCAGCAGCGGTCAGTCCGCGGATGCCCACTCC
ATCACCACGGCTGCCATGGAGTTCGGCGCGCTTGAAATCATTCCCGTGGTGATCACTTTC
AGCAACGTGCCCTCGGTCAGGCGCGCATTTGCGATTGACGACACTGGCACATTTCAAGTA
ATAGTGGTTCCCTCCGGGGCCGACTACATACCAGCATTAGAGAGACTCCAGCGGTGCACT
TTCTGCTATGATGTGTGCAAGCCAGATGCTTCTTGTGACCAAGCCAGACCACCCCCCGTG
CAGTCTTACATGGATGCTGCTTTCCTTCTGGATGCCTCCAGGAACATGGGAAGTGCTGAA
TTTGAAGACATAAGGGCCTTCCTTGGAGCACTATTAGATCACTTTGAAATCACCCCAGAG
CCGGAGACTTCTGTCACTGGCGACCGGGTGGCCCTATTGAGCCATGCTCCCCCCGACTTC
CTACCCAACACTCAGAAGAGTCCAGTTAGAGCTGAGTTCAATCTTACCACCTACAGAAGT
AAGCGCCTCATGAAGAGGCATGTGCACGAGTCAGTTAAACAACTAAATGGAGATGCTTTT
ATTGGTCATGCCTTACAGTGGACTCTCGACAATGTATTTTTGAGTACACCCAATCTGAGA
AGAAACAAAGTCATATTTGTGATATCTGCTGGGGAAACCAGCCACTTAGATGGGGAAATC
TTAAAGAAGGAATCCTTGCGAGCCAAATGTCAGGGATATGCCTTATTTGTGTTTTCCCTT
GGCCCTATTTGGGATGACAAGGAACTGGAGGATCTCGCCAGCCACCCTTTGGATCACCAC
CTGGTCCAGCTTGGCCGAATTCATAAACCTGACCACAGTTATGGTGTGAAGTTTGTGAAG
TCCTTTATAAACTCCATCAGGCGTGCAATCAACAAATATCCACCCATAAACTTAAAAATA
AAGTGCAACAGACTTAACTCTATAGATCCAAAGCAGCCCCCAAGACCATTCCGAAGCTTT
GTTCCTGGACCACTTAAAGCTACCCTCAAAGAAGATGTGTTACAGAAGGCCAAATTCTTT
CAAGATAAAAAATACCTTTCAAGAGTGGCAAGAAGTGGCAGAGATGATGCCATTCAAAAT
TTTATGAGAAGCACCTCCCATACCTTTAAGAATGGAAGGATGATAGAAAGTGCTCCCAAA
CAACATGAT
Protein sequence predicted based on nucleotide sequence
MMLLILFLVIICSHISVNQDSGPEYADVVFLVDSSDRLGSKSFPFVKMFITKMISSLPIE ADKYRVALAQYSDKLHSEFHLSTFKGRSPMLNHLRKNFGFIGGSLQIGKALQEAHRTYFS APANGRDKKQFPPILVVLASSESEDNVEEASKALRKDGVKIISVGVQKASEENLKAMATS QFHFNLRTVRDLSMFSQNMTHIIKDVIKYKEGAVDDIFVEACQGPSMADVVFLLDMSING SEENFDYLKGFLEESVSALDIKENCMRVGLVAYSNETKVINSLSMGINKSEVLQHIQNLS PRTGKAYTGAAIKKLRKEVFSARNGSRKNQGVPQIAVLVTHRDSEDNVTKAAVNLRREGV TIFTLGIEGASDTQLEKIASHPAEQYVSKLKTFADLAAHNQTFLKKLRNQITHTVSVFSE RTETLKSGCVDTEEADIYLLIDGSGSTQATDFHEMKTFLSEVVGMFNIAPHKVRVGAVQY ADSWDLEFEINKYSNKQDLGKAIENIRQMGGNTNTGAALNFTLSLLQKAKKQRGNKVPCH LVVLTNGMSKDSILEPANRLREEHIRVYAIGIKEANQTQLREIAGEEKRVYYVHDFDALK DIRNQVVQEICTEEACKEMKADIMFLVDSSGSIGPENFSKMKTFMKNLVSKSQIGPDRVQ IGVVQFSDINKEEFQLNRFMSQSDISNAIDQMAHIGQTTLTGSALSFVSQYFSPTKGARP NIRKFLILITDGEAQDIVKEPAVVLRQEGVIIYSVGVFGSNVTQLEEISGRPEMVFYVEN FDILQRIEDDLVFGICSPREECKRIEVLDVVFVIDSSGSIDYDEYNIMKDFMIGLVKKAD VGKNQVRFGALKYADDPEVLFYLDDFGTKLEVISVLQNDQAMGGSTYTAEALGFSDHMFT EARGSRLNKGVPQVLIVITDGESHDADKLNATAKALRDKGILVLAVGIDGANPVELLAMA GSSDKYFFVETFGGLKGIFSDVTASVCNSSKVDCEIDKVDLVFLMDGSTSIQPNDFKKMK EFLASVVQDFDVSLNRVRIGAAQFSDTYHPEFPLGTFIGEKEISFQIENIKQIFGNTHIG AALREVEHYFRPDMGSRINTGTPQVLLVLTDGQSQDEVAQAAEALRHRGIDIYSVGIGDV DDQQLIQITGTAEKKLTVHNFDELKKVNKRIVRNICTTAGESNCFVDVVVGFDVSTQEKG QTLLEGQPWMETYLQDILRAISSLNGVSCEVGTETQVSVAFQVTNAMEKYSPKFEIYSEN ILNSLKDITVKGPSLLNANLLDSLWDTFQNKSAARGKVVLLFSDGLDDDVEKLEQKSDEL RKEGLNALITVALDGPADSSDLADLPYIEFGKGFEYRTQLSIGMRELGSRLSKQLVNVAE RTCCCLFCKCIGGDGTMGDPGPPGKRGPPGFKGSEGYLGEEGIAGERGAPGPVGEQGTKG CYGTKGPKGNRGLNGQEGEVGENGIDGLNGEQGDNGLPGRKGEKGDEGSQGSPGKRGTPG DRGAKGLRGDPGAPGVDSSIEGPTGLKGERGRQGRRGWPGPPGTPGSRRKTAAHGRRGHT GPQGTAGIPGPDGLEGSLGLKGPQGPRGEAGVKGEKGGVGSKGPQGPPGPGGEAGNQGRL GSQGNKGEPGDLGEKGAVGFPGPRGLQGNDGSPGYGSVGRKGAKGQEGFPGESGPKGEIG DPGGPGETGLKGARGKMISAGLPGEMGSPGEPGPPGRKGVKGAKGLASFSTCELIQYVRD RSPGRHGKPECPVHPTELVFALDHSRDVTEQEFERMKEMMAFLVRDIKVRENSCPVGAHI AILSYNSHARHLVRFSDAYKKSQLLREIETIPYERSSASREIGRAMRFISRNVFKRTLPG AHTRKIATFFSSGQSADAHSITTAAMEFGALEIIPVVITFSNVPSVRRAFAIDDTGTFQV IVVPSGADYIPALERLQRCTFCYDVCKPDASCDQARPPPVQSYMDAAFLLDASRNMGSAE FEDIRAFLGALLDHFEITPEPETSVTGDRVALLSHAPPDFLPNTQKSPVRAEFNLTTYRS KRLMKRHVHESVKQLNGDAFIGHALQWTLDNVFLSTPNLRRNKVIFVISAGETSHLDGEI LKKESLRAKCQGYALFVFSLGPIWDDKELEDLASHPLDHHLVQLGRIHKPDHSYGVKFVK SFINSIRRAINKYPPINLKIKCNRLNSIDPKQPPRPFRSFVPGPLKATLKEDVLQKAKFF QDKKYLSRVARSGRDDAIQNFMRSTSHTFKNGRMIESAPKQHD
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
