Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
None
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00744821
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 4665nts
Open reading frame: 1 to 4665
ATGTCAGAGCAGACGCCGGCCGAGGCCGGTGCTGCGGGGGCCCGGGAGGACGCCTGTCGG
GATTATCAGTCATCGCTCGAAGACCTGACCTTCAATAGCAAGCCGCACATCAATATGCTG
ACCATTCTAGCCGAGGAGAACCTGCCCTTCGCCAAGGAGATCGTCTCTCTCATCGAGGCC
CAAACCGCCAAGGCTCCTTCCTCAGAGAAGCTTCCTGTTATGTACCTTATGGATTCTATT
GTGAAAAACGTTGGAAGAGAGTATCTCACTGCCTTTACTAAAAATCTAGTTGCAACATTT
ATTTGTGTGTTTGAAAAGGTGGATGAAAATACTAGGAAAAGTTTATTTAAGTTACGTTCT
ACATGGGATGAAATATTCCCTTTGAAGAAACTTTATGCCCTGGATGTCAGAGTCAATTCA
TTAGATCCTGCTTGGCCTATTAAACCTCTACCCCCCAATGTGAATACGTCTAGCATCCAT
GTGAATCCTAAATTTTTAAATAAATCGCCCGAGGAGCCTTCAACACCTGGTACAGTGGTC
AGTTCCCCTAGCATCTCCACTCCTCCAATTGTTCCTGATATACAAAAGAATCTTACACAA
GAACAACTAATAAGGCAGCAGTTACTGGCAAAGCAAAAACAGTTGTTAGAACTTCAGCAG
AAAAAGCTGGAGCTTGAGCTAGAGCAAGCTAAGGCACAGTTGGCAGTTTCTCTTAGTGTT
CAGCAGGAAACATCCAATTTAGGTCCTGGATCTGCACCATCCAAATTACATGTTTCACAG
ATTCCCCCTATGGCAGTTAAAGCTCCTCATCAGGTTCCTGTGCAATCTGAGAAAAGCCGT
CCAGGACCATCCTTACAAATTCAGGATTTAAAAGGAACTAACCGGGATCCTCGTCTGAAC
AGGATAAGCCAACATTCTCATGGAAAAGATCAGAGTCACAGGAAAGAATTTCTAATGAAC
ACATTAAACCAGTCTGATACTAAGACAAGTAAAACTATACCCTCTGAAAAACTAAATTCA
TCCAAGCAAGAAAAAAGTAAATCAGGTGAAAAAATAACCAAGAAAGAACTTGACCAATTA
GATTCTAAATCGAAATCGAAATCGAAATCACCCTCACCTTTGAAAAACAAATTATCTCAC
ACAAAAGACTTGAAAAATCAAGAATCGGAAAGTATGAGGTTGTCTGATATGAACAAGAGA
GATCCAAGATTAAAAAAACATCTTCAGGATAAGACCGATGGCAAAGATGATGATGTGAAA
GAGAAGAGAAAAACTGCAGAAAAAAAGGATAAAGATGAGCACATGAAGTCATCCGAACAC
AGACTGGCTGGAAGTAGAAATAAAATCATAAATGGCATTGTACAAAAACAGGATACAATA
ACAGAAGAGTCAGAAAAACAGGGGACAAAACCAGGGAGATCGAGTACTAGAAAGCGATCA
AGATCTCGATCACCCAAGTCTAGGTCACCAATTATACATTCCCCAAAGAGAAGAGATAGG
CGGTCACCCAAACGAAGGCAAAGAAGTATGTCTCCAACATCGACACCTAAAGCTGGAAAG
ATTCGCCAATCTGGAGCTAAGCAGTCACATATGGAAGAGTTTACACCACCTTCTAGGGAA
GACAGAAATGCTAAGAGAAGTACTAAACAGGATATTCGGGATCCAAGGCGAATGAAAAAG
ACTGAAGAGGAGCGACCACAAGAAACTACAAATCAGCATTCTACAAAGTCAGGCACTGAA
CCAAAGGAGAATGTAGAAAACTGGCAAAGTTCCAAGTCTGCCAAAAGATGGAAATCTGGT
TGGGAAGAAAATAAAAGCTTACAACAGGTTGATGAACATAGTAAACCTCCTCATCTGAGG
CATAGGGAGAGCTGGTCAAGCACTAAAGGAATTTTATCACCTCGAGCCCCAAAGCAGCAA
CAGCATCGATTAAGTGTAGATGCCAATCTTCAGATTCCTAAAGAGTTAACTCTTGCAAGC
AAAAGAGAATTACTTCAAAAGACGAGTGAACGTTTAGCATCTGGTGAAATTACACAGGAT
GACTTCCTTGTTGTTGTGCATCAAATTCGACAGCTATTTCAGTATCAAGAAGGTGTGCGA
GAAGAGCAGAGATCTCCATTCAATGATCGTTTTCCACTTAAGCGACCTCGATATGAAGAT
TCAGATAAACCATTTGTAGATAGTCCAGCATCAAGATTCGCCGGCCTGGATACAAATCAG
CGACTTACAGCTTTAGCTGAAGACAGACCGTTATTTGATGGACCTAGTAGGCCATCAGTA
GCAAGAGATGGCCCAACGAAGATGATTTTTGAAGGACCCAATAAATTAAGCCCTCGAATT
GATGGACCTCCCACACCAGCTTCTCTTCGGTTTGATGGGTCACCAGGACAAATGGGGGGA
GGAGGCCCTTTGAGATTTGAGGGGCCACAAGGTCAGCTAGGAGGTGGGTGTCCTTTGAGA
TTTGAAGGTCCTCCAGGACCAGTGGGGACACCTCTGCGGTTTGAGGGCCCAATTGGTCAA
GCAGGAGGAGGTGGTTTTCGGTTTGAAGGTTCCCCTGGTCTGAGGTTTGAGGGATCTCCA
GGTGGTTTGAGATTTGAGGGACCAGGAGGCCAGCCTGTGGGTGGTCTGAGGTTTGAGGGA
CATCGTGGTCAACCTGTGGGTGGTCTAAGGTTTGAGGGACCTCATGGTCAGCCTGTGGGT
GGACTTAGATTTGATAATCCCCGAGGTCAGCCTGTAGGTGGACTTAGATTTGAGGGGGGT
CATGGTCCATCAGGGGCTGCGATTAGGTTTGATGGACCTCATGGTCAGCCAGGAGGTGGA
ATCAGATTTGAGGGCCCTTTGCTACAGCAAGGGGTTGGAATGAGGTTTGAGGGCCCCCAT
GGTCAGTCAGTAGCTGGTCTGAGATTTGAGGGACAACATAATCAACTTGGTGGGAACCTT
AGGTTTGAGGGTCCACATGGTCAGCCAGGGGTTGGTATCAGGTTTGAAGGCCCTTTAGTC
CAACAAGGAGGTGGAATGAGGTTTGAGGGTCCTTCTGTACCAGGAGGTGGCCTGAGAATT
GAAGGGCCTCTGGGTCAAGGTGGTCCAAGATTTGAAGGTTGTCATGCTTTAAGGTTTGAT
GGGCAGCCAGGTCAGCCGTCACTCTTGCCAAGATTTGATGGATTACATGGTCAGCCAGGT
CCTAGATTTGAAAGGACTCCTGGTCAGCCAGGCCCTCAGAGGTTTGATGGACCACCTGGA
CAGCAGGTTCAACCCAGATTTGACGGTGTACCTCAAAGATTTGATGGTCCACAACATCAG
CAAGCATCAAGGTTTGATATTCCTCTTGGTCTTCAAGGCACAAGATTTGACAATCATCCT
TCACAAAGGCTTGAATCAGTATCTTTCAATCAGACTGGTCCATATAATGATCCACCTGGC
AATGCTTTTAATGCCCCATCCCAAGGACTACAGTTCCAAAGACATGAACAAATATTTGAT
TCACCTCAAGGACCAAATTTTAATGGACCACATGGCCCTGGAAACCAGAGTTTCTCTAAT
CCACTTAACAGAGCTTCTGGACACTATTTTGATGAAAAAAATCTTCAGAGTTCTCAATTT
GGAAACTTTGGCAATATACCTGCTCCAATGACAGTAGGAAATATTCAGGCATCTCAACAG
GTTCTGAGTGGTGTTGCTCAGCCAGTAGCTTTTGGTCAAGGACAACAGTTTTTACCAGTT
CATCCACAAAATCCTGGATTTGTTCAGAATCCTTCAGGAGCCCTCCCTAAGGCATATCCT
GATAATCATCTCAGTCAGGTGGATGTAAATGAATTGTTTTCAAAATTGCTAAAAACAGGA
ATTCTCAAATTGTCCCAAACTGATTCAGCTACAACACAAGTAAGTGAAGTAACTGCTCAG
CCTCCCCCTGAAGAGGAGGAAGATCAAAATGAAGATCAAGATGTTCCAGATCTTACTAAT
TTTACAGTTGAAGAATTGAAACAACGTTATGACAGTGTTATAAATCGACTGTACACTGGT
ATTCAGTGTTACTCTTGTGGAATGAGGTTTACAACATCACAGACAGATGTTTATGCAGAT
CATTTGGACTGGCATTATCGGCAAAATAGAACTGAAAAGGATGTTAGCCGAAAAGTCACT
CATAGACGTTGGTACTACAGTTTAACAGACTGGATAGAATTTGAGGAGATAGCTGATCTG
GAAGAACGGGCAAAGAGCCAGTTTTTTGAAAAGGTGCATGAAGAAGTTGTGCTCAAAACT
CAAGAGGCTGCTAAAGAAAAAGAGTTCCAAAGTGTACCTGCTGGACCAGCTGGAGCAGTT
GAGAGTTGTGAAATCTGTCAAGAACAATTTGAACAATACTGGGATGAAGAAGAGGAGGAA
TGGCATTTGAAAAATGCTATTAGAGTAGATGGAAAGATTTATCATCCATCATGTTATGAA
GATTATCAAAATACATCTTCATTTGATTGTACACCATCTCCCAGCAAGACACCAGTTGAA
AACCCCTTGAATATTATGTTGAACATTGTCAAAAACGAATTGCAGGAACCCTGTGACAGT
CCCAAAGTTAAGGAAGAACGAATTGATACACCACCAGCTTGTACAGAGGAAAGCATAGCA
ACACCCTCTGAAATTAAAACAGAAAATGACACAGTCGAGTCAGTT
Protein sequence predicted based on nucleotide sequence
MSEQTPAEAGAAGAREDACRDYQSSLEDLTFNSKPHINMLTILAEENLPFAKEIVSLIEA QTAKAPSSEKLPVMYLMDSIVKNVGREYLTAFTKNLVATFICVFEKVDENTRKSLFKLRS TWDEIFPLKKLYALDVRVNSLDPAWPIKPLPPNVNTSSIHVNPKFLNKSPEEPSTPGTVV SSPSISTPPIVPDIQKNLTQEQLIRQQLLAKQKQLLELQQKKLELELEQAKAQLAVSLSV QQETSNLGPGSAPSKLHVSQIPPMAVKAPHQVPVQSEKSRPGPSLQIQDLKGTNRDPRLN RISQHSHGKDQSHRKEFLMNTLNQSDTKTSKTIPSEKLNSSKQEKSKSGEKITKKELDQL DSKSKSKSKSPSPLKNKLSHTKDLKNQESESMRLSDMNKRDPRLKKHLQDKTDGKDDDVK EKRKTAEKKDKDEHMKSSEHRLAGSRNKIINGIVQKQDTITEESEKQGTKPGRSSTRKRS RSRSPKSRSPIIHSPKRRDRRSPKRRQRSMSPTSTPKAGKIRQSGAKQSHMEEFTPPSRE DRNAKRSTKQDIRDPRRMKKTEEERPQETTNQHSTKSGTEPKENVENWQSSKSAKRWKSG WEENKSLQQVDEHSKPPHLRHRESWSSTKGILSPRAPKQQQHRLSVDANLQIPKELTLAS KRELLQKTSERLASGEITQDDFLVVVHQIRQLFQYQEGVREEQRSPFNDRFPLKRPRYED SDKPFVDSPASRFAGLDTNQRLTALAEDRPLFDGPSRPSVARDGPTKMIFEGPNKLSPRI DGPPTPASLRFDGSPGQMGGGGPLRFEGPQGQLGGGCPLRFEGPPGPVGTPLRFEGPIGQ AGGGGFRFEGSPGLRFEGSPGGLRFEGPGGQPVGGLRFEGHRGQPVGGLRFEGPHGQPVG GLRFDNPRGQPVGGLRFEGGHGPSGAAIRFDGPHGQPGGGIRFEGPLLQQGVGMRFEGPH GQSVAGLRFEGQHNQLGGNLRFEGPHGQPGVGIRFEGPLVQQGGGMRFEGPSVPGGGLRI EGPLGQGGPRFEGCHALRFDGQPGQPSLLPRFDGLHGQPGPRFERTPGQPGPQRFDGPPG QQVQPRFDGVPQRFDGPQHQQASRFDIPLGLQGTRFDNHPSQRLESVSFNQTGPYNDPPG NAFNAPSQGLQFQRHEQIFDSPQGPNFNGPHGPGNQSFSNPLNRASGHYFDEKNLQSSQF GNFGNIPAPMTVGNIQASQQVLSGVAQPVAFGQGQQFLPVHPQNPGFVQNPSGALPKAYP DNHLSQVDVNELFSKLLKTGILKLSQTDSATTQVSEVTAQPPPEEEEDQNEDQDVPDLTN FTVEELKQRYDSVINRLYTGIQCYSCGMRFTTSQTDVYADHLDWHYRQNRTEKDVSRKVT HRRWYYSLTDWIEFEEIADLEERAKSQFFEKVHEEVVLKTQEAAKEKEFQSVPAGPAGAV ESCEICQEQFEQYWDEEEEEWHLKNAIRVDGKIYHPSCYEDYQNTSSFDCTPSPSKTPVE NPLNIMLNIVKNELQEPCDSPKVKEERIDTPPACTEESIATPSEIKTENDTVESV
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
