Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
Codon optimized
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pGW_NcoI-Eco109I
Format:
FUSION
Source:
None
Description:
None
Comments:
Gene synthesis by Gen9. Codon optimized
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00744880
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 6051nts
Open reading frame: 1 to 6051
ATGAGCTTGGGGCTGGCGGGGGCACAAGAGGTAGAGCTGACATTAGAGACGGTTATCCAG
ACACTAGAGAGCAGCGTCCTATGCCAAGAGAAAGGCTTGGGAGCGCGGGACCTGGCCCAG
GACGCTCAGATCACCAGCCTGCCTGCCCTCATCAGGGAAATTGTTACCCGAAACCTCTCC
CAGCCTGAGAGCCCAGTACTGCTTCCGGCCACAGAGATGGCATCGCTGCTGTCACTACAG
GAAGAGAACCAACTGCTGCAACAGGAGCTGTCCCGCGTGGAGGACCTACTGGCCCAGAGC
CGGGCCGAGCGAGATGAGCTCGCCATTAAGTACAATGCGGTTAGCGAGAGGCTTGAGCAG
GCACTGCGACTGGAGCCTGGAGAGTTGGAGACGCAGGAGCCCAGGGGGCTGGTACGGCAA
AGCGTAGAGTTGCGTAGGCAGCTGCAAGAGGAGCAAGCATCCTACCGGCGCAAGCTACAG
GCCTACCAGGAGGGCCAGCAGCGTCAGGCCCAGCTTGTGCAGAGGCTACAGGGCAAGATT
CTCCAGTACAAGAAGAGGTGCTCGGAGCTGGAACAACAGCTGCTAGAGAGATCCGGAGAG
CTGGAGCAGCAGCGGCTGAGGGACACAGAGCACAGCCAAGACCTTGAAAGCGCCCTCATC
CGACTAGAGGAGGAGCAACAGAGGAGTGCAAGCCTGGCCCAGGTGAATGCCATGCTCCGA
GAACAGCTGGACCAGGCAGGCTCGGCCAACCAGGCTCTTAGTGAGGACATACGAAAGGTG
ACCAATGACTGGACACGCTGCCGCAAGGAGCTAGAGCACCGGGAGGCGGCGTGGAGGCGC
GAGGAGGAGTCATTCAACGCCTACTTCAGTAACGAGCACAGTCGCCTGTTACTCCTCTGG
CGACAGGTGGTGGGGTTCCGGCGGCTGGTCAGCGAGGTGAAGATGTTCACTGAGAGGGAC
CTGCTGCAATTGGGAGGGGAGCTGGCTCGAACATCACGAGCTGTACAGGAGGCGGGCCTT
GGACTGAGCACGGGACTACGTTTGGCAGAGAGTCGTGCCGAGGCAGCTCTGGAGAAACAG
GCTCTGCTGCAAGCCCAGCTCGAGGAGCAGCTGCGGGACAAGGTACTCCGTGAGAAAGAC
CTGGCGCAGCAGCAGATGCAAAGCGACCTGGATAAGGCTGACCTCAGTGCCAGAGTGACA
GAGCTAGGCCTGGCAGTGAAGCGTCTTGAGAAACAGAATCTAGAGAAGGATCAGGTCAAC
AAGGACCTCACTGAGAAGTTAGAAGCCCTGGAATCCCTGCGGTTACAGGAACAGGCGGCC
CTGGAGACAGAGGATGGAGAGGGGCTACAGCAGACCCTAAGGGACCTGGCACAGGCCGTC
TTGTCAGACTCTGAGAGCGGAGTTCAGCTGAGCGGCTCTGAGCGCACCGCTGATGCTTCC
AATGGCAGCTTGCGGGGACTTTCTGGCCAGCGAACCCCATCACCACCGCGAAGATCATCG
CCCGGTAGAGGCAGATCACCTCGACGAGGCCCTTCACCGGCATGCTCAGACTCTTCCACG
CTAGCCTTGATCCACTCGGCCCTGCACAAGCGCCAGCTACAGGTCCAGGACATGCGTGGG
CGCTATGAGGCAAGCCAGGACCTATTGGGCACCCTTCGGAAGCAGCTTAGCGACAGTGAG
AGCGAGCGGCGTGCCCTAGAGGAACAGCTGCAGCGACTACGGGACAAGACAGACGGCGCC
ATGCAAGCTCACGAGGATGCCCAACGAGAGGTTCAACGGCTGCGGAGCGCTAACGAGCTC
CTGAGCAGGGAGAAGAGCAACCTAGCTCACAGCCTGCAAGTGGCCCAGCAGCAGGCAGAG
GAACTGAGGCAGGAGCGAGAGAAGCTGCAGGCTGCACAGGAGGAACTGCGGCGACAACGT
GACAGACTGGAGGAAGAGCAGGAGGACGCAGTGCAGGATGGCGCTAGGGTGCGACGGGAA
CTGGAGCGTAGCCATAGACAACTAGAGCAACTGGAAGGGAAGCGCTCAGTCCTGGCCAAG
GAGTTGGTTGAGGTAAGGGAGGCGCTTAGCCGCGCCACACTGCAACGGGACATGCTCCAG
GCAGAGAAGGCCGAGGTAGCCGAAGCGCTGACCAAGGCTGAGGCTGGTAGAGTAGAACTC
GAGCTCTCCATGACCAAGCTGAGAGCAGAGGAGGCCTCCCTGCAGGACTCCCTGTCCAAG
CTTAGCGCACTCAACGAGAGCCTTGCTCAGGACAAGTTGGATCTGAACCGCCTTGTTGCC
CAGCTGGAAGAAGAAAAGTCAGCCCTTCAGGGCCGGCAACGGCAAGCAGAGCAAGAGGCC
ACAGTGGCTCGGGAAGAACAGGAACGGCTAGAGGAGCTCCGGTTGGAACAGGAGGTTGCG
CGGCAAGGATTGGAGGGCTCCCTACGAGTTGCGGAGCAGGCCCAGGAGGCATTAGAACAG
CAGCTACCCACGTTGCGTCATGAGCGCAGCCAGTTGCAGGAGCAGCTAGCACAGCTCTCC
CGTCAGCTAAGCGGGCGGGAGCAGGAACTTGAACAGGCAAGGCGCGAAGCCCAGAGGCAA
GTGGAGGCTCTTGAGAGAGCTGCACGTGAGAAGGAGGCGCTAGCTAAGGAGCACGCTGGC
CTGGCTGTACAGTTGGTGGCAGCGGAGCGCGAAGGCAGGACACTGTCAGAAGAGGCAACA
CGCCTGCGATTGGAGAAGGAAGCACTGGAGGGCAGCCTGTTTGAAGTACAACGACAGCTA
GCCCAGTTAGAGGCCCGCCGGGAACAGCTTGAAGCCGAGGGGCAGGCCCTGCTACTAGCC
AAGGAGACACTAACTGGGGAATTGGCGGGCCTTCGACAGCAAATAATAGCAACACAGGAG
AAAGCCAGTCTAGATAAGGAGCTGATGGCCCAGAAACTGGTGCAGGCTGAGCGAGAAGCC
CAGGCCTCTCTGAGGGAGCAGCGGGCAGCACACGAGGAGGACTTACAGCGACTTCAGCGT
GAAAAGGAAGCAGCATGGCGGGAGTTGGAAGCCGAACGAGCCCAACTGCAGAGTCAGCTG
CAGCGTGAGCAGGAGGAGCTGCTTGCCCGGCTTGAGGCTGAGAAGGAAGAGTTGAGCGAG
GAGATTGCTGCCCTGCAGCAGGAGCGTGACGAGGGCCTCCTACTAGCAGAAAGTGAGAAG
CAGCAAGCCTTGTCTCTGAAGGAGTCTGAGAAGACGGCGCTGTCAGAGAAGTTGATGGGT
ACACGGCACAGCCTGGCTACCATCTCCCTAGAGATGGAGCGGCAGAAACGAGATGCCCAG
AGTCGACAGGAGCAGGACCGAAGCACCGTAAACGCTCTGACTTCTGAGTTGCGGGATCTA
AGGGCCCAGAGGGAGGAGGCTGCTGCGGCCCACGCTCAGGAGGTGAGAAGGCTACAAGAA
CAGGCCCGTGACCTAGGAAAGCAGCGAGACTCCTGTCTTCGAGAGGCGGAAGAGCTTCGG
ACACAGCTGCGTCTGCTAGAAGATGCCCGTGACGGGCTTCGGCGTGAGCTACTGGAGGCC
CAGCGAAAACTGCGTGAGAGTCAGGAAGGCCGTGAGGTACAACGCCAAGAGGCAGGCGAG
TTGCGACGAAGTCTAGGTGAGGGTGCCAAGGAGCGGGAAGCCCTACGGCGGTCTAATGAG
GAGCTTCGATCAGCTGTGAAGAAAGCAGAGAGCGAACGCATCAGCCTTAAGCTAGCCAAT
GAGGACAAGGAGCAGAAGCTGGCACTCCTAGAGGAGGCACGGACAGCCGTGGGCAAGGAG
GCAGGGGAGCTAAGAACTGGACTGCAAGAGGTGGAACGTTCACGTCTGGAAGCTCGGCGG
GAGCTGCAGGAGCTACGGCGTCAGATGAAGATGCTGGACAGTGAGAACACAAGACTGGGC
CGGGAGCTTGCTGAGCTACAGGGACGCCTGGCGCTGGGCGAGCGTGCAGAAAAGGAGAGC
AGGCGGGAAACACTGGGCCTTAGGCAAAGATTGCTAAAGGGCGAGGCCAGCCTGGAGGTG
ATGCGGCAGGAACTACAGGTAGCCCAGCGGAAGCTTCAGGAACAAGAAGGCGAGTTCCGG
ACCCGTGAGCGACGCTTGCTGGGTTCCCTGGAGGAGGCTCGTGGTACTGAAAAGCAGCAG
CTTGACCACGCCCGAGGCCTAGAGTTGAAGCTAGAGGCGGCGCGGGCTGAGGCTGCAGAA
TTGGGACTGCGGTTAAGCGCAGCTGAAGGCAGAGCACAAGGCCTGGAGGCAGAGTTGGCC
CGTGTTGAAGTGCAACGTCGCGCGGCGGAGGCTCAGCTAGGTGGCTTACGATCTGCTTTA
CGTAGGGGACTTGGCCTAGGTCGCGCGCCTAGTCCAGCTCCTAGACCAGTTCCCGGATCT
CCTGCCCGGGATGCACCAGCAGAAGGAAGTGGGGAAGGGTTGAACAGCCCCAGCACCTTA
GAATGCAGCCCTGGGTCCCAGCCACCATCTCCAGGACCTGCTACCTCTCCAGCCTCTCCA
GACTTGGATCCGGAGGCAGTGAGAGGGGCACTCCGGGAATTCCTGCAAGAGCTGAGGAGC
GCCCAGAGAGAACGGGACGAACTTCGGACCCAGACCAGTGCCCTGAATCGCCAGCTTGCC
GAGATGGAGGCTGAAAGGGACAGCGCAACATCGAGGGCCAGGCAGCTACAGAAGGCGGTG
GCTGAGAGTGAGGAAGCCAGGCGCAGTGTGGATGGGCGGTTAAGTGGTGTTCAGGCGGAG
TTGGCGCTGCAGGAAGAAAGTGTGAGGCGTAGTGAGAGGGAGCGTCGAGCCACGCTTGAC
CAGGTTGCCACACTGGAGAGAAGTCTGCAGGCAACCGAGAGCGAGCTTCGGGCCAGCCAG
GAGAAGATCAGCAAGATGAAGGCCAATGAGACAAAGCTGGAGGGCGACAAGCGACGCCTG
AAGGAGGTTTTGGACGCCTCCGAGAGTCGCACTGTAAAGCTGGAACTGCAGCGGCGCTCG
CTTGAGGGGGAGTTGCAGCGAAGCCGCCTGGGACTAAGTGACCGAGAGGCTCAAGCTCAG
GCCTTACAGGATCGGGTGGATTCCTTGCAGAGACAGGTTGCTGACAGCGAAGTGAAGGCA
GGGACCCTACAGCTGACCGTGGAGCGGTTGAATGGGGCCCTGGCTAAGGTGGAGGAAAGC
GAGGGGGCTCTGCGGGATAAGGTACGTGGACTGACAGAGGCCCTGGCTCAGAGCAGTGCT
AGCCTCAACAGTACCCGGGACAAGAACCTGCATCTGCAGAAGGCTCTGACCGCCTGTGAA
CATGACCGCCAAGTACTCCAGGAACGACTAGATGCCGCCCGGCAGGCATTGTCTGAGGCA
CGAAAACAGAGCAGCTCGCTAGGCGAGCAGGTTCAGACGTTGCGAGGTGAGGTGGCTGAC
CTAGAACTGCAACGTGTGGAGGCCGAGGGCCAGTTACAACAGCTACGTGAGGTGCTACGG
CAGCGGCAGGAGGGTGAGGCTGCCGCTTTGAACACCGTCCAGAAGTTGCAAGACGAGCGA
CGGCTGCTTCAGGAGCGATTGGGAAGCTTGCAGCGCGCCCTTGCTCAACTAGAAGCAGAG
AAGCGGGAGGTGGAGCGTTCAGCCCTGCGGCTGGAAAAGGACCGAGTAGCCCTTAGGAGG
ACGTTGGACAAGGTGGAGCGGGAGAAGCTTCGCAGCCATGAGGACACAGTGCGGTTGAGC
GCAGAGAAAGGCCGATTGGACCGCACCCTCACGGGGGCTGAATTAGAGCTGGCAGAGGCG
CAAAGGCAGATCCAGCAGCTGGAGGCGCAGGTAGTTGTGTTGGAGCAGAGTCACAGCCCG
GCACAACTGGAGGTGGATGCTCAGCAGCAACAGTTGGAGCTTCAGCAGGAAGTGGAGCGG
CTACGCAGCGCCCAAGCGCAGACTGAGCGTACCCTAGAGGCTAGGGAGCGGGCTCACCGA
CAGAGAGTACGTGGTCTTGAGGAGCAAGTGTCCACACTTAAGGGCCAGCTGCAGCAAGAA
CTTCGAAGGAGCTCAGCACCATTCTCCCCACCCTCCGGCCCCCCAGAGAAA
Protein sequence predicted based on nucleotide sequence
MSLGLAGAQEVELTLETVIQTLESSVLCQEKGLGARDLAQDAQITSLPALIREIVTRNLS QPESPVLLPATEMASLLSLQEENQLLQQELSRVEDLLAQSRAERDELAIKYNAVSERLEQ ALRLEPGELETQEPRGLVRQSVELRRQLQEEQASYRRKLQAYQEGQQRQAQLVQRLQGKI LQYKKRCSELEQQLLERSGELEQQRLRDTEHSQDLESALIRLEEEQQRSASLAQVNAMLR EQLDQAGSANQALSEDIRKVTNDWTRCRKELEHREAAWRREEESFNAYFSNEHSRLLLLW RQVVGFRRLVSEVKMFTERDLLQLGGELARTSRAVQEAGLGLSTGLRLAESRAEAALEKQ ALLQAQLEEQLRDKVLREKDLAQQQMQSDLDKADLSARVTELGLAVKRLEKQNLEKDQVN KDLTEKLEALESLRLQEQAALETEDGEGLQQTLRDLAQAVLSDSESGVQLSGSERTADAS NGSLRGLSGQRTPSPPRRSSPGRGRSPRRGPSPACSDSSTLALIHSALHKRQLQVQDMRG RYEASQDLLGTLRKQLSDSESERRALEEQLQRLRDKTDGAMQAHEDAQREVQRLRSANEL LSREKSNLAHSLQVAQQQAEELRQEREKLQAAQEELRRQRDRLEEEQEDAVQDGARVRRE LERSHRQLEQLEGKRSVLAKELVEVREALSRATLQRDMLQAEKAEVAEALTKAEAGRVEL ELSMTKLRAEEASLQDSLSKLSALNESLAQDKLDLNRLVAQLEEEKSALQGRQRQAEQEA TVAREEQERLEELRLEQEVARQGLEGSLRVAEQAQEALEQQLPTLRHERSQLQEQLAQLS RQLSGREQELEQARREAQRQVEALERAAREKEALAKEHAGLAVQLVAAEREGRTLSEEAT RLRLEKEALEGSLFEVQRQLAQLEARREQLEAEGQALLLAKETLTGELAGLRQQIIATQE KASLDKELMAQKLVQAEREAQASLREQRAAHEEDLQRLQREKEAAWRELEAERAQLQSQL QREQEELLARLEAEKEELSEEIAALQQERDEGLLLAESEKQQALSLKESEKTALSEKLMG TRHSLATISLEMERQKRDAQSRQEQDRSTVNALTSELRDLRAQREEAAAAHAQEVRRLQE QARDLGKQRDSCLREAEELRTQLRLLEDARDGLRRELLEAQRKLRESQEGREVQRQEAGE LRRSLGEGAKEREALRRSNEELRSAVKKAESERISLKLANEDKEQKLALLEEARTAVGKE AGELRTGLQEVERSRLEARRELQELRRQMKMLDSENTRLGRELAELQGRLALGERAEKES RRETLGLRQRLLKGEASLEVMRQELQVAQRKLQEQEGEFRTRERRLLGSLEEARGTEKQQ LDHARGLELKLEAARAEAAELGLRLSAAEGRAQGLEAELARVEVQRRAAEAQLGGLRSAL RRGLGLGRAPSPAPRPVPGSPARDAPAEGSGEGLNSPSTLECSPGSQPPSPGPATSPASP DLDPEAVRGALREFLQELRSAQRERDELRTQTSALNRQLAEMEAERDSATSRARQLQKAV AESEEARRSVDGRLSGVQAELALQEESVRRSERERRATLDQVATLERSLQATESELRASQ EKISKMKANETKLEGDKRRLKEVLDASESRTVKLELQRRSLEGELQRSRLGLSDREAQAQ ALQDRVDSLQRQVADSEVKAGTLQLTVERLNGALAKVEESEGALRDKVRGLTEALAQSSA SLNSTRDKNLHLQKALTACEHDRQVLQERLDAARQALSEARKQSSSLGEQVQTLRGEVAD LELQRVEAEGQLQQLREVLRQRQEGEAAALNTVQKLQDERRLLQERLGSLQRALAQLEAE KREVERSALRLEKDRVALRRTLDKVEREKLRSHEDTVRLSAEKGRLDRTLTGAELELAEA QRQIQQLEAQVVVLEQSHSPAQLEVDAQQQQLELQQEVERLRSAQAQTERTLEARERAHR QRVRGLEEQVSTLKGQLQQELRRSSAPFSPPSGPPEK
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
NGS Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pGW_NcoI-Eco109I
Synonyms:
pGW-NcoEco
Description:
In-fusion cloning vector with NcoI (5'att) and Eco109I (3'att) sites inserted to remove death cassette; kanamycin resistance in bacteria; recombinational cloning
Comments:
NcoI (5'att) and Eco109I (3'att) sites inserted to remove death cassette. Made for use with In-Fusion cloning
Size (bp):
4008
Parent Vector:
None
Properties:
''
Author Name:
Joshua LaBaer
Andreas Rolf
Andreas Rolf
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 bacterial origin
3332
4005
death cassette
ccdB
ccdB death cassette - removed in plasmids containing an insert
1779
2084
primer
M13 forward
M13 forward sequencing primer
536
553
primer
M13 reverse
M13 reverse sequencing primer
2269
2289
recombination site
attL1
attL1 recombinational cloning site
644
668
recombination site
attL2
attL2 recombinational cloning site
2133
2157
selectable marker
KanR
kanamycin resistance
2402
3208
selectable marker
CmR
Chloramphenicol resistance - removed in plasmids containing an insert
778
1434
